• Title/Summary/Keyword: starter development

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Development of a Novel Medium with Chinese Cabbage Extract and Optimized Fermentation Conditions for the Cultivation of Leuconostoc citreum GR1 (폐배추 추출물을 이용한 Leuconostoc citreum GR1 종균 배양용 최적 배지 및 배양 조건 개발)

  • Moon, Shin-Hye;Chang, Hae-Choon;Kim, In-Cheol
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.42 no.7
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    • pp.1125-1132
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    • 2013
  • In the kimchi manufacturing process, the starter is cultured on a large-scale and needs to be supplied at a low price to kimchi factories. However, current high costs associated with the culture of lactic acid bacteria for the starter, have led to rising kimchi prices. To solve this problem, the development of a new medium for culturing lactic acid bacteria was studied. The base materials of a this novel medium consisted of Chinese cabbage extract, a carbon source, a nitrogen source, and inorganic salts. The optimal composition of this medium was determined to be 30% Chinese cabbage extract, 2% maltose, 0.25% yeast extract, and $2{\times}$ salt stock (2% sodium acetate trihydrate, 0.8% disodium hydrogen phosphate, 0.8% sodium citrate, 0.8% ammonium sulfate, 0.04% magnesium sulfate, 0.02% manganese sulfate). The newly developed medium was named MFL (medium for lactic acid bacteria). After culture for 24 hr at $30^{\circ}C$, the CFU/mL of Leuconostoc (Leuc.) citreum GR1 in MRS and MFL was $3.41{\times}10^9$ and $7.49{\times}10^9$, respectively. The number of cells in the MFL medium was 2.2 times higher than their number in the MRS media. In a scale-up process using this optimized medium, the fermentation conditions for Leuc. citreum GR1 were tested in a 2 L working volume using a 5 L jar fermentor at $30^{\circ}C$. At an impeller speed of 50 rpm (without pH control), the viable cell count was $8.60{\times}10^9$ CFU/mL. From studies on pH-stat control fermentation, the optimal pH and regulating agent was determined to be 6.8 and NaOH, respectively. At an impeller speed of 50 rpm with pH control, the viable cell count was $11.42{\times}10^9(1.14{\times}10^{10})$ CFU/mL after cultivation for 20 hr - a value was 3.34 times higher than that obtained using the MRS media in biomass production. This MFL media is expected to have economic advantages for the cultivation of Leuc. citreum GR1 as a starter for kimchi production.

Effects of Litter Type and Gender on Behavior Characteristics and Growth Performance of Korean Hanhyup Broiler (한국형 한협 육계의 행동 특성과 성장성에 미치는 Litter Type과 Gender의 영향)

  • Yang, Ka Young;Ha, Jae Jung;Roh, Hee-Jong;Cho, Chang-Yeon;Oh, Seung Min;Oh, Dong-Yep
    • Korean Journal of Poultry Science
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    • v.46 no.3
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    • pp.155-160
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    • 2019
  • The Korean Hanhyup broiler has commercialized native meat-type chicken. This study aimed to determine the effects of two types of litter material-sawdust and rice husk, and gender on the Korean Hanhyup broiler. Chicks (n=416) were randomly assigned in a $2{\times}2$ factorial design by type of litter material and gender. The behavior of chicken's was monitored continuously for 12 hours during the $1^{st}$ through $8^{th}$ week of age. The time spent on standing, sitting, and walking and the frequency of drinking, feeding, preening, dust bathing, flapping, and aggressive pecking, and pecking of chicken's behavior were recorded. The behavior of chickens during the starter (1 to 4 weeks) and finisher period (5 to 8 weeks) was compared to observe the changes upon maturity. Our results indicate that litter material type and gender have no effect on the behavior of chickens. However, aggressive pecking and pecking caused increment in the rice husk as compared to sawdust (P<0.05). Sawdust as litter material for bedding was found to be better than rice husk with respect to Korean Hanhyup broiler behavior.

Biogenic Amine Degradation by Bacillus Species Isolated from Traditional Fermented Soybean Food and Detection of Decarboxylase-Related Genes

  • Eom, Jeong Seon;Seo, Bo Young;Choi, Hye Sun
    • Journal of Microbiology and Biotechnology
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    • v.25 no.9
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    • pp.1519-1527
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    • 2015
  • Biogenic amines in some food products present considerable toxicological risks as potential human carcinogens when consumed in excess concentrations. In this study, we investigated the degradation of the biogenic amines histamine and tyramine and the presence of genes encoding histidine and tyrosine decarboxylases and amine oxidase in Bacillus species isolated from fermented soybean food. No expression of histidine and tyrosine decarboxylase genes (hdc and tydc) were detected in the Bacillus species isolated (B. subtilis HJ0-6, B. subtilis D'J53-4, and B. idriensis RD13-10), although substantial levels of amine oxidase gene (yobN) expression were observed. We also found that the three selected strains, as non-biogenic amineproducing bacteria, were significantly able to degrade the biogenic amines histamine and tyramine. These results indicated that the selected Bacillus species could be used as a starter culture for the control of biogenic amine accumulation and degradation in food. Our study findings also provided the basis for the development of potential biological control agents against these biogenic amines for use in the food preservation and food safety sectors.

Development of a Rupture Disk for Pyrostarters (파이로스타터용 럽쳐디스크 개발)

  • Park, Ho-Jun;Hong, Moon-Geun;Lee, Soo-Yong
    • Proceedings of the Korean Society of Propulsion Engineers Conference
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    • 2009.05a
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    • pp.219-222
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    • 2009
  • Pyrostarters play a role as a turbopump starter in liquid propellant propulsion systems by supplying pressurized gas to power turbines for engine start. A rupture disk in pyrostarters, which is usually installed behind a nozzle throat, not only isolates the charged solid propellants from the external environment but also improves the ignitability of the solid propellants by increasing a chamber pressure at the beginning of combustion. Experimental tests have been performed to study the effects of rupture disk thickness, depth and shape of scores, and pressure build-up rates on burst pressures and burst diameters. The experimental results show that the developed rupture disk fulfills the performance requirements expected in a real operational condition.

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The Effect of Angelica gigas Water Extract on the Growth of Lactic Acid Bacteria (당귀가 유산균의 생육에 미치는 영향)

  • Oh Youn-Jeong;Lee Su-Han;Jung Seung-Won;Noh Wan-Seob
    • Journal of the East Asian Society of Dietary Life
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    • v.16 no.3
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    • pp.344-348
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    • 2006
  • This study was carried out to survey the effect of medicinal herbs on the growth of 3 strains of lactic starter cultures in MRS broth by the addition of 0, 1, 3, 5, and 10% water extract, after 30 hrs incubation. The pH, titratable acidity and O.D. of lactic acid bacteria were investigated to obtain fundamental knowledge for the development a new product. The effects of medicinal herbs extracts on the growth of lactic acid bacteria were variable depending upon the species of lactic acid bacteria and medicinal herbs extract. Growth of Str. thermohilus was activated by addition of 1 and 3% Angelica gigas water extract, but the basal medium plus 5 and 10% extract and growth of L. acidophilus and Bif. longum were depressed by the addition of extract. The pH and acid production ability dropped slightly in the basal medium plus 1 and 3% of extract. The other samples following the same trend as the control.

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A Study on Effect of Acanthopanax sessiliflorum Seeman Water Extract on the Growth of Lactic Acid Bacteria (오갈피(Acanthopanax sessiliflorum Seeman) 추출액이 유산균의 생육에 미치는 영향)

  • Kim Sun-Young;Park Keun-Sil;Lee Su-Han;Jung Seung-Won;Noh Wan-Seob
    • Journal of the East Asian Society of Dietary Life
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    • v.16 no.3
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    • pp.357-363
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    • 2006
  • This study examined the effect of medicinal herbs on the growth of 3 strains of lactic starter cultures in a MRS broth by adding a 0, 1, 3, 5, and 10% water extract. The pH, titratable acidity and OD of lactic acid bacteria were investigated in order to obtain fundamental knowledge for the development a new product, box thorn yogurt. In the case of adding the Acanthopanax sessiliflorum Seeman water extract, Streptococcus thermophilus was largely activated by the addition of the 5% extract. The growth of Lactobacillus acidophilus was activated by the extracts but it was depressed by the addition of the 3% Acanthopanax sessiliflorum Seeman water extract. Compared with the control, the growth of Bifidobacterium longum was largely activated by the addition of the 10% extract but it was depressed by the addition of the 5% Acanthopanax sessiliflorum Seeman water extract.

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Development of Functional Yogurts Prepared with Mulberries and Mulberry Tree Leaves

  • Lee, An-Cheol;Hong, Youn-Ho
    • Food Science of Animal Resources
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    • v.30 no.4
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    • pp.649-654
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    • 2010
  • In order to develop new functional yogurts using mulberries and mulberry leaves, which were cultivated in Hwasun-gun, Jeonnam Province, Korea, the nutritional compositions, fermentation conditions, sensory properties, and storage stabilities of the yogurts were analyzed. The mulberry powder yogurt contained 87.96% moisture, 3.21% carbohydrate, 4.52% protein, 3.63% lipid, and 0.68% ash, and the mulberry leaf yogurt contained 86.36% moisture, 4.13% carbohydrate, 4.87% protein, 3.79% lipid, and 0.85% ash. A yogurt base was fermented for 13 h with 0.01% ABT-5 starter inoculum at $40^{\circ}C$. To prepare the mulberry jam and mulberry leaf yogurts, a variety of mulberry jam and mulberry leaf samples were added to the yogurt base. The sensory evaluation results of the yogurts containing the mulberry jam and mulberry leaves indicated that a product made with 15% mulberry jam was more strongly preferred than other samples. When the mulberry jam and mulberry leaf yogurts were stored at $4^{\circ}C$ for 15 d, there were no significant changes in pH, titratable acidity, or viable cell numbers of lactic acid bacteria and Bifidobacterium bifidum.

The Role of Vitamins and Minerals in the Production of High Quality Pork - Review -

  • Mahan, D.C.;Kim, Y.Y.
    • Asian-Australasian Journal of Animal Sciences
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    • v.12 no.2
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    • pp.287-294
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    • 1999
  • Vitamin and mineral deletion from swine diets can result in reduced growth if done during the period wher muscle and bone development is occurring. Several of the vitamins and minerals decline in the serum during the starter period, suggesting a higher dietary inclusion may be necessary postweaning. Vitamin research with grower-finisher pigs is limited, but results suggest that rapidly growing lean pigs may have a higher dietary requirement for the B vitamins. Several studies have suggested that early weaning and pigs of a lean genotype may have a dietary requirement for vitamin C, CI and Cr. High dietary vitamin E levels are fortified in the diet and seems to be effective in preventing mulberry heart problems in weanling and grower pigs. Organic Se is more effectively retained in muscle tissue than inorganic Se, approximately 20% less is excreted, but the bioavailability of organic Se for glutathione peroxidase activity is only 80 to 90% to that of sodium selenite. The active form of thyroxine (T4) is dependent upon a Se containing enzyme. Withdrawal of vitamins and minerals during the latter part of the finisher period has not affected pig performance responses, but studies with poultry suggest that the vitamin content of the meat may be reduced if the vitamins are withdrawn prior to marketing. High levels of vitamin E have been shown to improve pork quality, by reducing drip loss. Studies with vitamin C and Se have suggested that they may also be involved in pork quality.

Development of Multilayer Perceptron Model for the Prediction of Alcohol Concentration of Makgeolli

  • Kim, JoonYong;Rho, Shin-Joung;Cho, Yun Sung;Cho, EunSun
    • Journal of Biosystems Engineering
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    • v.43 no.3
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    • pp.229-236
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    • 2018
  • Purpose: Makgeolli is a traditional alcoholic beverage made from rice with a fermentation starter called "nuruk." The concentration of alcohol in makgeolli depends on the temperature of the fermentation tank. It is important to monitor the alcohol concentration to manage the makgeolli production process. Methods: Data were collected from 84 makgeolli fermentation tanks over a year period. Independent variables included the temperatures of the tanks and the room where the tanks were located, as well as the quantity, acidity, and water concentration of the source. Software for the multilayer perceptron model (MLP) was written in Python using the Scikit-learn library. Results: Many models were created for which the optimization converged within 100 iterations, and their coefficients of determination $R^2$ were considerably high. The coefficient of determination $R^2$ of the best model with the training set and the test set were 0.94 and 0.93, respectively. The fact that the difference between them was very small indicated that the model was not overfitted. The maximum and minimum error was approximately 2% and the total MSE was 0.078%. Conclusions: The MLP model could help predict the alcohol concentration and to control the production process of makgeolli. In future research, the optimization of the production process will be studied based on the model.

Development of Wound Rotor Synchronous Motor for Belt-Driven e-Assist System

  • Lee, Geun-Ho;Lee, Heon-Hyeong;Wang, Qi
    • Journal of Magnetics
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    • v.18 no.4
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    • pp.487-493
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    • 2013
  • The automotive industry is showing widespread interest in belt-driven electric motor-assisted (e-Assist) systems. A belt-driven assist system (BAS) starts and assists the combustion engine in place of the conventional generator. In this study, a water-cooled wound rotor synchronous motor (WRSM) for the e-Assist system was designed and analyzed. The performance of the WRSM was compared with that of an interior permanent magnet synchronous motor (IPMSM). The WRSM efficiency can be improved for the BAS by adjusting the field flux at high speeds. The field current map to obtain the maximum efficiency based on the speed and torque was developed. To control the field flux via field current control in the WRSM, a general H-bridge circuit was added to the WRSM inverter to get the rapid current response in the high-speed region; the characteristics were compared with the chopper circuit. A WRSM developed for the belt-driven e-Assist system and a prototype 115 V power electronic converter to drive the WRSM were tested with a 900 cc combustion engine. The test results showed that the WRSM-type e-Assist system had good characteristics and could successfully start and assist the 900 cc combustion engine.