• Environmental Analysis Health and Toxicology
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• v.18 no.2
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• pp.77-87
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• 2003

성게의 정자를 이용한 생물검정은 해양환경의 독성평가에 널리 사용되고 있다. 그러나, 국내산 성게류의 경우 산란기가 3~11월로 겨울에는 생물검정 실칩이 불가능한 단점이 있다. 이를 보완하기 위하여 겨울에 산란이 가능한 아무르불가사리(Arterias amurensis)를 생물검정용으로 개발하였다. 불가사리류의 생식과 발생과정은 성게류와 유사하므로 성게류를 이용한 실험 방법을 아무르불가사리에 적용할 수 있다. 그러나, 생물검정 실험 결과의 유효성은 실험조건에 의하여 달라질 수 있으므로, 본 연구에서는 실험 결과에 큰 영향을 미칠 수 있는 조건들 즉. 수정막의 발달 시간, 정자와 알의 비율, 정자의 활력이 유지되는 시간, 정자의 노출 시간, 그리고 시료의 염분 범위 등을 최적화 하고자 하였다. 수정막의 발달이 완전하게 이루어지는 최소 시간은 60분이었고, 시료에 주입할 정자와 알의 비율은 3,000이상일 때 수정률이 높게 유지되었다(70%이상). 정자의 활력은 해수에 희석한 후 3시간 이상이 경과하면 급격히 감소하였다. 정자의 노출시간은 20~60분의 범위에서 구리에 대한 $EC_{50}$이 크게 달라지지 않았다(변동계수: 17%).실험에 적절한 시료의 염분 범위는 26~38 psu이었다. 아무르불가사리 정자의 민감도는, 일반적으로 해양환경 평가에 흔히 사용되는 생물들의 민감도 범위의 중간 정도에 해당하였다. 따라서 아무르불가사리의 정자는 해양환경 평가를 위한 생물검정용으로 적합한 것으로 여겨진다.

• Fisheries and Aquatic Sciences
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• v.12 no.1
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• pp.1-5
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• 2009

Crude saponin, extracted from the starfish Asterias amurensis, was assessed for its capacity as a biological pesticide. As part of this analysis, its insecticidal and repellent activities, in addition to its acute and chronic toxicities were tested. In comparison with the control group, insecticidal activity of saponin against tobacco cutworm, Spodoptera litura, in kale, Brassica loeracea was low at 36.4%. Repellent activities of the extracted saponin against green peach aphid, Myzus persicae, and S. litura, on soybean leaf, Glycine max and kale were 65.6% and 35.0% at $1^{st}$ day, and 54.5% and 30.0% at $3^{rd}$ day, respectively. Acute and chronic toxicity analysis was carried out using acute immobilizatioin test and reproduction impairment test, respectively. The saponin had 48 h-$EC_{50}$ of $65.21{\mu}g/mL$. Twenty-one day accumulative reproduction after treatment was lower in Daphnia magna at $7{\mu}g/mL$ saponin (78 youngs), compared with the control group (129 youngs). These results indicate that the extracted saponin exhibited some toxicity and has potential as a repellent against insects.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.33 no.3
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• pp.166-172
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• 1997

In order to exterminate effectively starfish, Asterias amurensis inhabited a village fishing grounds and shellfish farms on coast of Korea, Mop and sledge gear were made and sea trials for capture efficiency of starfish by each gear and towing distance were carried out by commercial dredger on the coast of Keojedo from April to May in 1995. As Starfish mop and sledge were slowly dragged over the bottom at the same time, starfish became entangled in bunches of twine and netting respectively. The gears were hauled up at intervals to remove the starfish and hand-picking operations on vessel were conducted. The results obtained are as follows : Two gears were smoothly slidden over the sea bottom and captured numerous starfish. The optimal towing distance by each gear was 300 to 500 m.The capture efficiency of starfish species by sledge was 57% compared with 43% of that by mop but mixed rate with fish or shellfish of the former was 21 times as high as that of the letter. It was concluded from sea trials that moping was effective in shellfish farms, because the mop outfit causes little damage to useful shellfishes and the mixture of starfish with fish or shellfish was low, whereas sledging can be used to clean uncultivated areas free of shellfish where starfish population is very heavy and common fishing grounds in which bottom material is rock or gravel.

• The Korean Journal of Malacology
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• v.16 no.1_2
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• pp.17-20
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• 2000

Effect of predation of starfish Asterias amurensis and Asterina pectinifera under different water temperature in the recirculating seawater vessel was conducted at the invertebrate culture laboratory of Yosu National University from February 1 to February 25, 2000. The highest activity of Asterias amurensis and Asterina pectinifera according to water temperature was obtained at 14$\^{C}$ and 22$\^{C}$. The average individual numbers of Anadara broughtonii predated by Asterias amurensis and Asterina pectinifera were 2.20 and 1.60 at 6$\^{C}$ , 3.60 and 2.00 at 10$\^{C}$, 7.20 and 2.50 at 14$\^{C}$, 3.40 and 6.80 at 22$\^{C}$, 0.80 and 3.90 at 26$\^{C}$, respectively.

• Journal of Microbiology and Biotechnology
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• v.28 no.3
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• pp.349-356
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• 2018

Asterias amurensis is a marine organism that causes damage to the fishing industry worldwide; however, it has been considered a promising source of functional components. The present study aimed to investigate the immune-enhancing effects of fatty acids from three organs of A. amurensis on murine macrophages (RAW 264.7 cells). A. amurensis fatty acids boosted production of immune-associated factors such as nitric oxide (NO) and prostaglandin E2 in RAW 264.7 cells. A. amurensis fatty acids also enhanced the expression of critical immune-associated genes, including iNOS, $TNF-{\alpha}$, $IL-1{\beta}$, and IL-6, as well as COX-2. Western blotting showed that A. amurensis fatty acids stimulated the $NF-{\kappa}B$ and MAPK pathways by phosphorylation of $NF-{\kappa}B$ p-65, p38, ERK1/2, and JNK. A. amurensis fatty acids from different tissues resulted in different levels of $NF-{\kappa}B$ and MAPK phosphorylation in RAW 264.7 cells. The results increase our understanding of how A. amurensis fatty acids boost immunity in a physiological system, as a potential functional material.

• Proceedings of the Korean Aquaculture Society Conference
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• 2004.05a
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• pp.379-380
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• 2004

• Food Science and Preservation
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• v.19 no.5
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• pp.727-734
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• 2012

For developing calcium supplement from the harmful marine organism starfish, the physiochemical property of the powdered starfish skeletal plate and the hydrolysis condition of the starfish digestive enzyme were studied. The optimal hydrolysis condition of the starfish digestive enzymes was at $55^{\circ}C$ for 12 h. The bulk densities of the powdered starfish skeletal plates of Asterias amurensis and Asterina pectinifera were $1.1{\pm}0.0$ and $1.2{\pm}0.0g/cm^3$, respectively. As for the median size, the values were 10.738 and $11.799{\mu}m$, respectively. According to the added concentration of sodium polyacrylate, the dispersibility rate of the powdered starfish skeletal plate was shown to be 6h at 0.0%, 3 days at 0.1%, 20 days at 0.2%, and until 30 days at 0.4%. The elementary composition of the powdered starfish skeletal plates from A. amurensis and A. pectinifera mainly consisted of calcium, and it formed 98.95 and 98.52% of the powdered starfish skeletal plates, respectively. The results of the X-ray diffraction (XRD) analysis showed that they were present in the form of $CaCO_3$. Based on these results, it is suggested that starfish skeletal plate can be utilized as a functional material for a calcium supplement.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.12
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• pp.410-417
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• 2019

The enhancement effects of sea mustard extract (SME), starfish collagen peptide (SCP) and a mixture of the two (MIX) on skin activity were evaluated to investigate the possibility of using marine-derived extracts as cosmetic additives. The anti-inflammatory activity, whitening activity and skin elasticity activity of the extracts were analyzed to evaluate their skin-activating effects. Inhibiting the generation of nitric oxide (NO) and the tyrosinase and elastase inhibitory activities were assessed as the bio-markers for evaluating skin activity. SME, SCP and MIX did not show cytotoxicity within the concentration range of 1.0-50 ㎍/mL. In addition, SME, SCP and MIX all increased NO production and the tyrosinase and elastase inhibitory activities in a concentration-dependent manner. The activity of MIX was significantly increased compared to that with using SME or SCP alone. Taken together, when natural extracts are applied as cosmetic additives, the results demonstrate that using a mixture of SME and SCP may have a greater synergistic effect than that when using only a single extract. Therefore, this study contributes to the knowledge about the kinds and composition of several natural extracts when they are used as cosmetic additives.