• 제목/요약/키워드: starch granule protein

검색결과 25건 처리시간 0.03초

Influence of Different Transplanting Dates on Amylopectin Branch-chain-length and Pasting Properties of Rice Flour Varieties

  • Han, Chae-Min;Shin, Jong-Hee;Kim, Sang-Kuk;Kwon, Tae-Young;KIM, Jong-Sang
    • 한국작물학회지
    • /
    • 제63권3호
    • /
    • pp.210-218
    • /
    • 2018
  • This study was conducted to examine the influence of different transplanting dates on rice quality and starch properties (morphology and pasting properties) of rice varieties that may be used for the production of rice flour. Three rice flour varieties, 'Seolgaeng', 'Hangaru', and 'Milyang317', were transplanted on May 20, May 30, June 10, and June 20. The peak viscosity decreased with a delay in the transplanting date. However, the amylose content increased with a delay in the transplanting date, whereas that of protein decreased. Amylopectin short-branch chain content increased in the rice varieties that were transplanted on May 30. The morphology of the starch granule of the varieties was determined by SEM. No apparent external difference in the starch granules was observed for the different transplanting dates. These results indicate that the transplanting date influenced the amylopectin structure and pasting properties, which led to changes in the physicochemical characteristics of rice starch.

기름골 전분의 이화학적 특성 (Physicochemical Properties of Chufa (Cyperus esculentus L., var sativus Boeck) Starch)

  • 한상하;이현유;금준석;박종대
    • 한국식품저장유통학회지
    • /
    • 제13권3호
    • /
    • pp.382-388
    • /
    • 2006
  • 기름골의 이용 가능성을 규명하고자 기름골의 일반성분 및 기름골 전분의 이화학적 특성을 조사하였다. 기름골의 수분함량은 5.4%, 조지방, 조단백질 함량은 각각 24.2, 7.2%로 비교적 많은 양의 지방을 함유하고 있었다. 전분특성과 관련하여 먼저 알카리 침지법에 의해서 전분을 분리하였다. 기름골 전분의 일반성분 결과 전분의 수분함량은 10.10%, 조지방, 조단백질 함량은 각각 0.41, 0.31%였다. 기름골 전분의 아밀로오스 함량은 41.6%였고 blue value는 0.49, 전분의 최대흡수파장은 628 nm로 조사되었다. 기름골 전분의 백도는 92.23이었으며 물 결합능력은 83%로 조사되었다. 팽윤력과 용해도의 측정결과 $60^{\circ}C$ 이하에서는 완만한 증가를 보이다가 $60^{\circ}C$를 경계로 $65^{\circ}C$까지 급격히 증가하였고 $70^{\circ}C$ 이후에는 다시 완만하게 증가하는 경향을 보였다. 주사전자현미경 관찰결과 기름골 전분의 입자형태는 표면이 매끄러운 원형 또는 타원형이었고 입자크기는 약 $10{\mu}m$정도였으며 기름골 생전분의 물결합능력은 83%였다. DSC에 의한 호화양상에서 호화개시온도는 $72.2^{\circ}C$, 호화최대온도는 $78.3^{\circ}C$, 호화종결온도는 $76.4^{\circ}C$로 나타나 DSC에 의해 측정된 호화온도가 RVA의 호화온도보다 낮았다. 기름골의 호화양상은 RVA와 DSC로 측정하였는데, RVA측정결과 최대 및 최종점도는 각각 385.08, 293.92 RVA로 나타났다.

발효과정 중 증편반죽의 가용성 단백질, 유리아미노산 및 전분의 변화 (Changes in Soluble Protein, Free Amino Acid and Starch of Jeungpyun Dough during Fermentation)

  • 박영선;서정식
    • 한국식품조리과학회지
    • /
    • 제11권3호
    • /
    • pp.282-286
    • /
    • 1995
  • 발효시간을 0-10시간으로 달리한 증편반죽에서 발효시간에 따른 이화학적 성질변화를 검토하였다. 가용성단백질과 유리아미노산 총량은 발효초기에 감소하여 발효 2시간과 4시간에 각각 최저치 0.292 mg/g-dry matter와 13.31 mg/100 g-dry matter를 기록하였으나 그 이후에는 다시 증가하였다. 발효 초기단계에서는 발효전 시료와의 차이를 볼 수 없으나 발효 6시간 이상의 반죽시료에서 전분의 결정화도가 감소되고 전분입자가 붕괴되는 현상이 다소 일어남이 X-ray 회절도와 주사형 전자현미경에 의하여 각각 확인되었다.

  • PDF

The Physicochemical Properties of Starch from Tongil-type Rice Varieties

  • Jong-Hee Shin;Chae-Min Han;Young-Un Song;Sang-Kuk Kim;Jung-Gi Ryu
    • 한국작물학회지
    • /
    • 제68권3호
    • /
    • pp.99-105
    • /
    • 2023
  • Starch characteristics and pasting properties of Tongil-type rice varieties with different amylose content were analyzed. Three different Tongil-type rice varieties and one Japonica-type rice variety were examined for their properties. Tongil-type rice varieties have longer panicles and higher rice yield (721-765 kg per 10a) than Boramchan, a Japonica-type high-yield rice variety. The protein content of the Tongil-type rice variety was higher than that of Boramchan. Japonica-type rice varieties had lower amylose content than Japonica-type rice varieties, except for Amimyeon (Milyang355). Amimyeon had higher protein content than the other varieties, and its amylose content was particularly high at 39.2%. The distribution of starch granule sizes of the four varieties was similar, and the particle size corresponding to D50 was approximately 87.8-81.9 ㎛. The pasting properties of rice flour varied among varieties. The Dasanbyeo and Hanarum2 amylogram patterns were similar. These two varieties had a higher peak viscosity (PV) and lower setback (SB) than Boramchan. In the case of Amimyeon, the hot paste viscosity (HPV), cool paste viscosity (CPV), and SB were significantly higher than those of the other Toingil-type varieties, and the breakdown (BD) was very low, showing pasting properties significantly different from that of the other varieties. The onset (To), peak (Tp), and conclusion temperature (Tc) of gelatinization of rice flour from Amimyeon were lower than those of the other tested varieties. In addition, the gelatinization temperature, measured using differnetial scanning calorimetry (DSC), and BD, measured using al rapid visco analyzer (RVA) were low in Amimyeon. Amylose content showed a high positive correlation with pasting time (PTi), HPV, and SB and a negative correlation with PV and BD.

흰찰쌀보리 가루를 이용한 제빵특성 연구 1. 흰찰쌀보리-밀가루 혼합분 박죽의 물성 (Study on Bread-making Quality with Mixture of Waxy Barley-Wheat Flour 1. Rheological Properties of Dough Made with Waxy Barley-Wheat Flour Mixture)

  • 유정희
    • 한국식품영양과학회지
    • /
    • 제28권5호
    • /
    • pp.1034-1043
    • /
    • 1999
  • Rheological properties of dough made from waxy barley(Iri28) flour wheat flour mixtures with additives were investigated for the preparation of waxy barley bread using farinograph, extensograph and amy lograph. The water absorption, development time and dough weakness increased as the waxy barley flour level increased in all blends; however, dough stability decreased. Farinogram properties of 10% waxy barley flour added mixture were similar to those of 100% wheat flour. The addition of A.A(ascorbic acid), gluten, HPMC(hydroxy propyl methyl cellulose) improved rheological properties of dough with 30% waxy barley flour added mixture. In particular, stability and weakness of the dough showed greater dough improving effect by addition of A.A. For the extensograph data, strength, resistance and extensibility of dough decreased with increasing level of waxy barley flour. With the addition of additives, extensogram properties were variable for 30% waxy barley flour mixture. Of these additives, gluten had highest value in strength of dough. Addition of A.A and HPMC to 30% waxy barley flour added mixture resulted in an increase in the resistance and a decrease in the extensibility. Waxy barley flour added mixtures showed little higher gelatinization temperature on amylograph data than control. Maximum viscosity reduced as the waxy barley flour level increased. Also 30% waxy barley flour added mixture containing A.A and HPMC showed a decrease in maximum viscosity. But addition of gluten to 30% waxy barley flour mixture resulted an increase in the maximum viscosity. All of 30% waxy barley flour added mixture with additives had lower gelatinization temperature than those without additives. In the SEM images, starch granules were dispersed in a protein matrix. A non continuous, loose protein starch matrix was observed in all waxy barley flour mixture by SEM. Addition of additives gave the dough a more continuous structure with interactions between the starch granule and protein component.

  • PDF

도토리에 대한 국내의 연구 동향 (A Study on the Literature Review of Acorn in Korea)

  • 김복남
    • 한국식품조리과학회지
    • /
    • 제11권2호
    • /
    • pp.158-163
    • /
    • 1995
  • This study was surveyed and compiled the contribution about acorn in korea .the physico-chemical properties of acorn and acorn starch. preparation of acorn starch and elimination of the acorn tannin, the rheological properties of acorn starch gel. Nutrition of acorn and antioxidative activity of tannin in acorn extract. The result obtained were as follow. 1. The chemical compositions of acorn were water 6.5∼13.7%, crude ash 1.9∼3.4%, crude fat 1.1∼5.0%, crude protein 5.8∼7.8%, crude fiber 2.1∼3.6%, N-free extract 71∼77.5%, total tannin 4.6∼9.3%, Ca 92.7∼460.9mg%, p 80.0∼740.9mg%, Na 66.2∼93.9 mg%, and K 867.9∼983.1mg%. 2. The acorn tannin was extracted with water, acetone, and ethanol. The generation method was wash SE settling method with water. 3. The shape of acorn starch granule was rounded triangular and some elliptical, rasing power 12.4∼12.5, Blue value 0.43∼0.47, Alkali number 10.8∼11.3, Amount of Arnylose 28.8∼30.50% Tannin contents on the initial go tim-zation temperature of acorn starch were not influenced but maximum and cooling viscosity o the acorn starch were decreased. 4. Nutritional effect and diet absorption rate were not influence, by rice and 20% T-A(elimination of tannin from the acorn) mixed diet, and the content of lipid in s rum were not influenced by rice 40% and T-A (elimination of tannin from the acorn) mixed diet. 5. jallic acid, digallic acid, gallotannin were contained in acorn powder extract. The main antioxidative : stlvity was speculated due to the gallic acid.

  • PDF

Analysis of Kernel Hardness of Korean Wheat Cultivars

  • Hong, Byung-Hee;Park, Chul-Soo
    • 한국작물학회지
    • /
    • 제44권1호
    • /
    • pp.78-85
    • /
    • 1999
  • To investigate kernel hardness, a compression test which is widely used to measure the hardness of individual kernels as a physical testing method was made simultaneously with the measurement of friabilin (15KDa) which is strongly associated with kernel hardness and was recently developed as a biochemical marker for evaluating kernel hardness in 79 Korean wheat varieties and experimental lines. With the scattered diagram based on the principal component analysis from the parameters of the compression test, 79 Korean wheat varieties were classified into three groups based on the principal component analysis. Since conventional methods required large amount of flour samples for analysis of friabilin due to the relatively small amount of friabilin in wheat kernels, those methods had limitations for quality prediction in wheat breeding programs. An extraction of friabilin from the starch of a single kernel through cesium chloride gradient centrifugation was successful in this experiment. Among 79 Korean wheat varieties and experimental lines 50 lines (63.3%) exhibited a friabilin band and 29 lines (36.7%) did not show a friabilin band. In this study, lines that contained high maximum force and the lower ratio of minimum force to maximum force showed the absence of the friabilin band. Identification of friabilin, which is the product of a major gene, could be applied in the screening procedures of kernel hardness. The single kernel analysis system for friabilin was found to be an easy, simple and effective screening method for early generation materials in a wheat breeding program for quality improvement.

  • PDF

Characteristics of Biochemical Markers and Whole-Wheat Flours Using Small-Scaled Sampling Methods in Korean Wheats

  • Park Chul Soo;Kim Yang-Kil;Han Ouk-Kyu;Lee Mi Ja;Park Jong-Chul;Seo Jae-Hwan;Hwang Jong-Jin;Kim Jung-Gon;Kim Tae Wan
    • 한국작물학회지
    • /
    • 제50권5호
    • /
    • pp.346-355
    • /
    • 2005
  • To investigate the application of biochemical markers' and small-sample methods using whole-wheat flours for screening in early generation in Korean wheat breeding system, 74 Korean wheats, including cultivars, local breeding lines and experimental lines, were analyzed. Seed storage protein and amylose contents of grains were evaluated. Biochemical makers, including granule bound starch synthase (GBSS), high molecular weigh glutenin subunits (HMW-GS) and friabilin were also evaluated by using one-dimensional sodium dodecyl sulfate-polyacryla-mide gel electrophoresis with a single kernel. The small­sample methods, including modified SDS-sedimentation test (MST), micro-alkaline water retention capacity (AWRC) and whole-wheat flour swelling volume (WSV) were also tested in this study. Protein content, MST and AWRC was $11.0 - 15.8\%$, 2.7 - 26.2 ml and $71.9 - 109.7\%$, respectively. Apparent and total amylose content and WSV was $20.6 - 25.0\%$, $26.1 - 32.4\%$ and 9.0 - 16.9 ml, respectively. There were highly significant correlations between MST and AWRC (r=0.592, P<0.001), but Korean wheats showed no significant difference in protein content, amylose content and small-sample methods. In the biochemical markers, Korean wheats contained all three GBSS encoded by Wx loci, except for Suwon 252. Korean wheats showed the high frequency ($58.1\%$) of 1Dx2.2 + 1Dy12 subunits of HMW-GS. Friabilin band was present in 46 lines ($62.2\%$) and absent in 28 lines ($37.8\%$). Friabilin-absence lines showed the higher MST (14.9 ml) and AWRC ($92.1\%$) value than friabilin-presence lines (8.5 ml and $82.4\%$, respectively).

수침기간에 따른 찹쌀가루와 찹쌀전분의 이화학적 및 구조적 특성 (Physicochemical and Structural Characteristics of Waxy Rice Flours and Starches during Soaking Time)

  • 박사라;노준희;신말식
    • 동아시아식생활학회지
    • /
    • 제26권5호
    • /
    • pp.457-465
    • /
    • 2016
  • Effects of soaking time on the physicochemical and structural characteristics of waxy rice flours and starches purified from flours using the alkaline steeping method were investigated. Korean cultivar Sinseonchal waxy rice was washed and soaked in tap water (1:2 w/w) and stored at room temperature for 15 days. On each day of soaking for 0, 1,2 3, 5, 10, and 15 days, pH of soaking water was measured and rice grains were dried, ground, and passed through 100 mesh sieve. The pH was reduced to 3.90 by day 5 and increased to 4.60 by day 15. The protein and ash contents, swelling powers and solubilities of flours and starches decreased with increasing soaking time. The water-binding capacities increased while trends were not similar to soaking time. The flour particle size distribution ranged from two to four peaks with increasing soaking times. Starch granule size decreased with increasing soaking time. The peak, trough, and final viscosities of flours and starches showed similar trends until 10 days and 15 days, respectively. The starches presented higher viscosities than the flours. The branch chain length distributions of amylopectin of starches showed an increaseed DP6~12 portion and decreased DP13~24 portion with increasing soaking time of waxy rice grains.

발효 전후 쌀뜨물의 일반성분 변화 (Proximate Compositions Changed Before and After Fermentation of Rice Spent Water)

  • 김민주;박성수;김동호;김근성
    • 한국식품위생안전성학회지
    • /
    • 제26권3호
    • /
    • pp.192-197
    • /
    • 2011
  • 생활하수의 주요 오염원으로 알려져 있는 쌀뜨물은 여러 가지 유익한 생리활성 성분을 함유하고 있어 여러 방면으로 재활용 될 수 있는 자원임에도 불구하고 실제로 활용도가 낮아 자원으로서 낭비가 되고 있다. 이러한 쌀뜨물의 활용도를 높이기 위하여 선행연구를 통하여 쌀뜨물 발효액이 이미 개발되었다. 그러므로 본 연구에서는 쌀뜨물의 발효 전, 후의 성분변화를 파악하기 위하여 쌀뜨물과 쌀뜨물 발효액의 일반성분 함량을 분석하고, 일반성분 내 탄수화물 및 단백질 유래 생리활성 성분 변화 정도를 파악하였다. 이와 같은 연구 결과는 향후에 쌀뜨물 발효액을 이용하여 쌀음료, 쌀비누, 쌀화장품 등과 같은 생활용품을 개발하는데 필요한 기초자료로 활용될 수 있을 것으로 사료된다. 일반성분 함량 분석 결과에 의하면 쌀뜨물과 쌀뜨물 발효액은 99.3%이상의 수분을 함유하고 있었으며, 탄수화물, 지방, 단백질 등의 고형 성분을 0.7% 정도 함유하고 있었다. 이는 쌀을 씻는 과정에서 쌀의 영양성분중 일부가 쌀뜨물로 이동한다는 것을 의미한다. 또한 쌀뜨물 발효액의 경우 발효전의 쌀뜨물과 비교하여 탄수화물 함량은 44.8% 감소하고, 조회분, 조단백질, 조지방 함량은 각각 36.6%, 16.4%, 그리고 18.8% 증가하였다. 실제로 주사 전자 현미경을 이용하여 starch 미세구조를 관찰한 결과에 의하면 쌀가루와 쌀뜨물 검체 내에서는 손상되지 않은 starch granule의 형태를 확인 할 수 있었지만 발효과정을 거친 쌀뜨물 발효액 검체 내에서는 starch가 가수분해 되어 starch granule의 형태를 거의 관찰할 수 없었다. D-형 및 L-형 lactic acid는 쌀가루와 발효전의 쌀뜨물 검체 내에는 존재하지 않았지만 발효과정을 거친 쌀뜨물 발효액 검체 내에서는 D-lactic acid가 212.13 g/kg, 그리고 L-lactic acid가 181.25 g/kg이 각각 측정되었다. 이와 같은 결과는 쌀뜨물 내의 탄수화물 성분이 발효되면서 D-형 및 L-형 lactic acid로 전환된 것으로 사료된다. 암모니아태 및 아미노태 질소함량은 발효과정을 거친 쌀뜨물 발효액 검체가 발효전 쌀뜨물 겸체 보다 각각 7배, 12배 정도 높게 측정되었다. 이와 같은 현상은 쌀뜨물 내의 단백질 유래 성분이 발효과정 중 protease 등에 의하여 가수분해가 되었음을 의미한다.