• 제목/요약/키워드: standard culture methods

검색결과 232건 처리시간 0.029초

식품원가 분류에 따른 산업체급식의 식단관리 및 영양공급량평가 (Evaluation of Menu Management and Nutrition Supply in Industrial Foodservice by Food Cost)

  • 박명희;최봉순
    • 한국식생활문화학회지
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    • 제8권2호
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    • pp.91-101
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    • 1993
  • Unit price of meal provision in industrial foodservice differs with each business. It is believed that menu management work, contents of menu, and nutrition supply performed by dietitian are different with unit price of meal provision. In this sense, purposes of this study are to examine meal management work and contents of menu, by food cost, at industrial foodservice in Taegu, Kyungbuk area and to evaluate variety of menu and nutrition supply. Results of the study are as follows: 1. Average number of side dishes provided at meal, including Kimchi, was 2 at low unit price, 3.0 at middle unit price and 3.1 at high unit price. 2. Possession ratio of account books using for menu management was low. 3. With regard to kind of menu, high unit price was most various and showed more various, compared to low unit price, especially in cooking oil-used cook methods such as broil, pan-fried food, fry, and roast. 4. High unit price was found as the highest in kind of used food and supply volume. 5. Sugar, Mineral, Vitamine, and Protein were satisfied with standard food content, but fat and calcium were not. 6. Supply volume of food was positively correlated to supply volume of nutrient, but kinds of cook methods and food type were not correlated to that of nutrient.

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Safety of Temporary Use of Recycled Autoclaved Femoral Components in Infected Total Knee Arthroplasty: Confirming Sterility Using a Sonication Method

  • Park, Hyung-Jin;Kim, Hee-June;Kim, Shukho;Kim, Seong-Min;Mun, Jong-Uk;Kim, Jungmin;Kyung, Hee-Soo
    • Clinics in Orthopedic Surgery
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    • 제10권4호
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    • pp.427-432
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    • 2018
  • Background: The purpose of this study was to evaluate the usefulness of sonication technique for microbiological diagnosis and the sterility of the recycled autoclaved femoral components from infected total knee arthroplasty (TKA) using a sonication method. Methods: Nineteen femoral implants explanted from patients with infected TKA were sterilized with a standard autoclave method. Standard culture of the fluid before and after sonication of the sterilized implants was performed to detect pathogenic microorganisms. Additional experiments were performed to evaluate the sterility of the recycled implant by inducing artificial biofilm formation. Methicillin-resistant Staphylococcus aureus (MRSA) was inoculated into 10 implants and sterilization in a standard autoclave was performed, and then the fluid was cultured before and after sonication. Results: Two of the 19 sterilized implants were positive for growth of bacteria after sonication, whereas no growth was detected in the cultured fluid from the sterilized implants before sonication. The bacteria were Staphylococcus species in all two cases. In one of 10 implants inoculated with MRSA, the culture was positive for growth of bacteria both before and after sonication. However, Staphylococcus epidermidis was cultured from both occasions and thus this implant was thought to be contaminated. Conclusions: We found sonication for identification of pathogens could be helpful, but this finding should be interpreted carefully because of the possibility of contamination. Sterilization of an infected femoral implant with an autoclave method could be a good method for using the temporary articulating antibiotic spacer in two-stage revision arthroplasty.

공동체 문화 활성화를 위한 APT단지 부대복리시설 계획에 관한 연구 (A Study on the Service Facilities Plan for APT Community Culture Activation)

  • 이광영;조진만
    • 한국주거학회논문집
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    • 제19권2호
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    • pp.55-62
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    • 2008
  • This study is researched to provide design guidelines of service facilities and programs that apartment residents want along with the increased quality of life. This study is signifiant because it sought effective methods to activate the com-munity culture through service facilities in apartment complexes and the design standard and planning direction for residents. The study results are as follows. In the result of the correlations analysis and multiple regression analysis for the importancy of service facilities in apartment complexes, the following orders were considered as important factor; man-ager office, open space, resident community facility, elderly people meeting and activity facility, walking paths, resident athletic facility, a children's playground, library, and rest areas in the complex. and result of the correlations analysis and multiple regression analysis is the following: service facilities whole importance (y) = 1.413$+0.262^*{\times}1$ (manager office importance)$+0.271^*{\times}2$ (open space importance) $+0.185^*{\times}3$ (resident community facility importance) $+0.136{\times}4$ (elderly people meeting and activity facility importance)$+0.036^*{\times}5$ (walking paths importance) $+0.031^*{\times}6$ (resident athletic facility importance) + $(-0.042)^*{\times}7$ (a children's playground importance) + $(-0.045)^*{\times}8$ (library importance) + $(-0.0097)^*{\times}9$ (rest areas importance).

검정곰팡이의 분화에 따르는 모노뉴클레오티드 성분의 분포 변동에 관한 연구 (Studies on the Distribution of the Mononucleotides in Aspergillus niger during Differentiation)

  • 김분래;김종협
    • 한국균학회지
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    • 제13권2호
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    • pp.65-74
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    • 1985
  • 1. 분화과정에 따른 균체로부터 추출한 총 RNA량은 포자형성 직전에 증가함을 알았다. 2. 추출한 RNA를 가수분해하여 P.E.I. Cellulose TLC를 전개한 결과 AMP유도체와 IMP가 존재함을 확인하였다. 3. 포자형성을 전후하여 IMP와 AMP의 RNA에 대한 함량비율은 일정함을 알았다. 4. 포자형성 전기의 균체에 아데노신, 구아노신, 이노신, 크산토신을 처리한바 포자형성이 10배 이상 촉진되었다. 5. 포자형성 전기의 균체에 A. bisporus, F. velutipes, L. edodes의 열수추출액을 처리한바 포자형성이 $1.5{\sim}2$배 촉진되었다.

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해산식품과 채소에서 Vibrio parahaemolyticus 검출을 위한 배지배양법과 real-time PCR의 비교검증 (Comparison of the Standard Culture Method and Real-time PCR for the Detection of Vibrio parahaemolyticus in Seafoods and Vegetables)

  • 천정환;현지연;황인균;곽효선;한정아;정윤희;송광영;서건호
    • 한국식품과학회지
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    • 제42권3호
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    • pp.355-360
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    • 2010
  • V. parahaemolyticus는 국내에서 여름과 가을철에 걸쳐 발생되며 생선이나 패류 등의 해산식품을 날것으로 섭취하거나 불완전하게 익혀 먹을 경우 식중독을 일으키는 병원균이다. 본 연구의 목적은 표준 검출기법인 배지배양법을 이용한 V. parahaemolyticus의 검출과 real-time PCR을 이용한 V. parahaemolyticus의 검출에 있어서 그 유효성과 효율성을 검증하는 것이다. V. parahaemolyticus의 발생기록과 발생가능성이 있는 여러 해산식품과 무순에 적절한 균량을 접종하고 APW로 증균배양하였다. 증균배양이 끝난 후 TCBS선택배지에 배양액을 획선도말하고, 동시에 증균배양액에서 1 mL을 채취하여 real-time PCR을 실시하였다. TCBS에서 초록색이 나온 집락을 1-3개 선별하여 TSI 배지에 접종하여 screening test를 거친 후 API 20NE strip을 사용하여 확인동정하였다. 또한 정상세균총이 목적균의 성장과 검출에 어느 정도의 영향을 미치는지 평가하기 위하여 25 g의 식품 내 정상세균총의 수준을 함께 측정하였다. 실험결과, 자체 제작한 real-time PCR 서열은 V. parahaemolyticus를 특이적으로 검출할 수 있었고 검출 한계는 PBS에서 $10^3\;CFU/mL$ 였다. 또한, 식품 내 정상세균총이 높을 경우 증균배양 시 목적균의 성장에 영향을 미칠 수 있다는 것을 확인하였다. Real-time PCR은 180개의 전체 샘플 중 76개의 양성 결과를 보여 66개의 양성 결과를 낸 배지배양법에 비해 더 많은 양성 검출율을 보였으나 통계학적인 유의차는 발견되지 않았다. Real-time PCR은 표준검출법인 배지배양법과 비교해 볼 때 동등하거나 우수한 검출력을 지닌 것으로 보이며 이러한 realtime PCR법은 24시간 이내에 확정동정까지 가능하여 시간과 노동력의 소모가 많은 배지배양법에 앞서 선별검사로 사용할 경우 시간, 비용, 노동력 절감에 매우 유효할 것으로 판단된다.

변조전달함수 방법에서 엣지 장치 설정에 대한 각도 최적화 평가 (Evaluation of Angle Optimization on Edge Test Device Setting in Modulation Transfer Function)

  • 민정환;정회원
    • 대한방사선기술학회지:방사선기술과학
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    • 제43권1호
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    • pp.15-21
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    • 2020
  • This study was purpose to evaluation of Modulation Transfer Function in Measurements by using the International electrotechnical commission standard(IEC 62220-1) which were edge device each angle by using edge method. In this study was Aero(Konica, Japan) image receptor which is a indirect Flat panel detector(FPD) was used. The size of matrix 1994 × 2430 (14"× 17" inch) which performed 12 bit processing and pixel pitch is 175 ㎛. The results of shown as MTF measurements at IEC standard. The amount of data seemed reasonable and at an MTF value of 0.1 the spatial frequencies were 2.56 cycles/mm at an angle of 2.4°. MTF value of 0.5 the spatial frequencies were 1.32 cycles/mm at an angle of 2.4°. This study were to evaluate MTF by setting each angle 2.0°~2.8° degrees the most effective optimal edge angle and to suggest the quantitative methods of measuring by using IEC.

만성 당뇨발에서 표재조직 및 심부조직 세균배양검사의 비교 (Difference of Microbiology according to Tissue Sampling in Diabetic Ulcers)

  • 이성미;한승규;김우경
    • Archives of Plastic Surgery
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    • 제37권1호
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    • pp.1-6
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    • 2010
  • Purpose: Diabetic foot infection is one of the most common and severe complications of diabetes mellitus that delays healing of the wound. Deep tissue biopsy is considered to be the gold standard method for antibiotic selection in treating infected chronic diabetic ulcers. However, swab culture or superficial tissue biopsy is often performed for a microbiologic test since deep tissue biopsy has limitations in application. The purpose of this study is to find out whether microbiologic results of swab culture or superficial tissue biopsy could be used for selection of antibiotics in treating diabetic ulcers. Methods: This study involved 42 patients with diabetic foot ulcers with negative results in bone probing test. Tissue samples for microbiologic tests were collected from all the patients by using superficial cotton swab, superficial tissue, and deep tissue. The microbiologic results of deep tissue biopsy were compared with swab culture and superficial tissue biopsy statistically. Results: Microbiology of the deep tissue showed the same results with those of the swab culture and superficial tissue in 67% and 71%, respectively. Statistical analysis demonstrated that the microbiology of the swab culture and superficial tissue does not coincide with that of the deep tissue. Conclusion: These results suggest that the microbiology of the swab culture and superficial tissue is not concordant with that of the deep tissue in infected chronic diabetic ulcers. To select appropriate antibiotic regimen, the speci specimen for the microbiologic test should be obtained from deep tissue.

'총각김치' 명칭의 시작과 확산, 그리고 보편화 과정 고찰: 음식문화 콘텐츠 관점을 연계하여 (A Study on the Origin, Spread, and Universalization of the Name 'Chonggak kimchi': In Connection with the Food Culture Content Point of View)

  • 김홍렬
    • 한국식생활문화학회지
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    • 제37권5호
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    • pp.418-428
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    • 2022
  • Research on the birth (起源) and names (語源) of foods such as kimchi is important to understand traditional food culture. kimchi, an 'add flavored, fermented, pickled, vegetable food' was initially prepared with the simple purpose of increasing storage capabilities, but later, through a complex process of change, morphological diversification occurred. In addition to the basic name of 'kimchi', each variety has its unique name and history. This study was conducted through qualitative research using various research methods, such as oral records and interviews, as well as investigation of data from literature, including ancient literature, modern cookbooks, newspapers, magazines, papers, and videos. The study sought to investigate the context and the meaning of the name Chonggak kimchi. In addition, it is a compilation of how the name spread through the ages and evolved to its current name. The name Chonggak kimchi did not exist during the Joseon Dynasty and Japanese occupation and first appeared in the records in the late 1950s. Nevertheless, the original name of 'Altarimu kimchi' evolved and finally became a part of the standard Korean language (標準語) in 1988. In the process of the name spreading and becoming popular, the movie "Chonggak kimchi (1964)," starring Shin, S.I., and Eom, A.R. played a significant role. It was also confirmed that this was a meaningful and valuable case of contentization of traditional food culture, regardless of the intention behind the same.

Optimization of Trichomonas vaginalis Diagnosis during Pregnancy at a University Hospital, Argentina

  • Testardini, Pamela;Vaulet, Maria Lucia Gallo;Entrocassi, Andrea Carolina;Menghi, Claudia;Eliseht, Martha Cora;Gatta, Claudia;Losada, Mirta;Touzon, Maria Sol;Corominas, Ana;Vay, Carlos;Tatti, Silvio;Famiglietti, Angela;Fermepin, Marcelo Rodriguez;Perazzi, Beatriz
    • Parasites, Hosts and Diseases
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    • 제54권2호
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    • pp.191-195
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    • 2016
  • The aim of this study was to evaluate different methods for Trichomonas vaginalis diagnosis during pregnancy in order to prevent maternal and perinatal complications. A total of 386 vaginal exudates from pregnant women were analyzed. T. vaginalis was investigated by 3 types of microscopic examinations direct wet mount with physiologic saline solution, prolonged May-Grunwald Giemsa (MGG) staining, and wet mount with sodium-acetate-formalin (SAF)/methylene blue method. PCR for 18S rRNA gene as well as culture in liquid medium were performed. The sensitivity and specificity of the microscopic examinations were evaluated considering the culture media positivity or the PCR techniques as gold standard. The frequency of T. vaginalis infection was 6.2% by culture and/or PCR, 5.2% by PCR, 4.7% by culture, 3.1% by SAF/methylene blue method and 2.8% by direct wet smear and prolonged MGG staining. The sensitivities were 83.3%, 75.0%, 50.0%, and 45.8% for PCR, culture, SAF/methylene blue method, and direct wet smear-prolonged MGG staining, respectively. The specificity was 100% for all the assessed methods. Microscopic examinations showed low sensitivity, mainly in asymptomatic pregnant patients. It is necessary to improve the detection of T. vaginalis using combined methods providing higher sensitivity, such as culture and PCR, mainly in asymptomatic pregnant patients, in order to prevent maternal and perinatal complications.

黃連解毒湯, 黃連, 黃芩, 黃柏, 梔子의 Salmonella Typhimurium에 대한 항균효과 (Antimicrobial Activity of Hwangryunheadok-tang and Extracts of Scutellariae Radix, Phellodendri Cortex, Coptis Rhizome, and Gardenia Jasminoides against Salmonella Typhimurium)

  • 김인수;이영수
    • 대한한방내과학회지
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    • 제36권2호
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    • pp.165-179
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    • 2015
  • Objectives: This experiment investigated the antimicrobial activity of Hwangryunheadok-tang and extracts of Scutellariae radix , Phellodendri cortex , Coptis rhizome , and Gardenia jasminoides against Salmonella typhimurium . Methods: After spreading S. typhimurium on a bacterial culture medium plate, antimicrobial activity was tested by dripping diluted Hwangryunheadok-tang or extracts of Scutellariae radix , Phellodendri cortex , Coptis rhizome , or Gardenia jasminoides (80 μl, diluted 100, 50, 10, and 1%) onto the plate, followed by culture for 16 to 72 hours. The minimal inhibitory concentration (MIC) was tested by dripping the minimum dilution that showed antimicrobial activity (80, 60, 40, and 20 μl) and measuring the density. The antimicrobial activity of Hwangryunheadok-tang and four extracts showed continuous antibacterial activity against S. typhimurium throughout the experiment. Result: 1. S. typhimurium . (Standard Microorganism, ATCC) 1) The Hwangryunheadok-tang and extracts of Scutellariae radix , Phellodendri cortex , and Coptis rhizome showed antibacterial activity in the undiluted solutions and in 50% dilutions. Gardenia jasminoides extract showed potency only in the undiluted solution. The antimicrobial potency of the undiluted solution was increased when the volume of inoculation was increased, but no difference was noted when the culture time was extended. All the extracts showed antimicrobial potency against S. typhimurium . 2. S. typhimurium isolated from diarrhea patients 1) When compared to the standard microorganism experiment on S. typhimurium , the MICs of the five extracts were increased. However, whereas the antimicrobial potency of doxycycline was lost entirely against bacteria isolated from patients with diarrhea, the antimicrobial potency of all the extracts was diminished but did not disappear. 2) The antimicrobial activity of Hwangryunheadok-tang and four extracts was continuous even when the culture time was extended to 16, 24, and 72 hours. Conclusions: The Hwangryunheadok-tang and four kinds of extracts have antimicrobial activity against S. typhimurium . Comparison of a standard microorganism with S. typhimurium isolated from diarrhea patients showed that the antimicrobial activity of all the extracts was better than that of antibiotics. Further studies should focus on the value and benefits of Hwangryunheadok-tang , and the four kinds of extracts as clinical treatments.