• Textile Coloration and Finishing
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• v.15 no.5
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• pp.285-293
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• 2003

Waterborne PU membrane was prepared from waterborne PU dispersion solution to investigate physical and staining properties. The staining properties of waterborne PU membrane with acid dyes and disperse dyes were observed. The physical properties of the PU membrane were investigated by X-ray diffraction and IR spectroscopy. The staining property of waterborne PU membrane for azo acid dyes is better than that of disperse dyes. X-ray diffraction peaks sharpened and tensile strain and stress increased with heat setting temperature.

• Journal of Biomedical Engineering Research
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• v.23 no.3
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• pp.235-241
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• 2002

The detection of tubercle bacilli (TB) from sputum smear is one of the fast and inexpensive methods for diagnosis of tuberculosis. For this method. sputum smears are usually flexed by heating and stained by acid-fast staining method, and then examined under an optical microscope. Two Procedures are commonly used fur TB staining. One is hot staining and the other is cold staining method. The Ziehl-Neelsen method which is a hot staining method is widely used in Korea because its stained color is more vivid However, the conventional automated stainer has to fix the sputum smear on a slide manually and the stain is not so vivid because it has not heating function. In an effort to save labor and minimize variations in manual staining Procedure. we developed an automated stainer with heating function. The entire staining process is fully automated. from fixation to final washing and drying. With the automated methods, five slides can be flexed and stained in 21 minutes at consistent high quality We compared the concordance rate between the two methods for 91 sputum samples to validate the stain quality of the developed automated stainer. As the results, the concordant rate between the two methods was 95% and there was no significant difference (p>0.05)

• Animal cells and systems
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• v.3 no.1
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• pp.53-58
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• 1999

The immunocytochemical method was used to identify the existence of voltage-dependent $Ca^{2＋}$-channels in mouse follicular oocytes. Three types of voltage-dependent $Ca^{2＋}$-channels were shown to exist in the follicular oocytes for the first time, the P/Q-type $Ca^{2＋}$-channel, the N-type $Ca^{2＋}$-channel, and the L-type $Ca^{2＋}$-channel. Among proven $Ca^{2＋}$-channels distributions of the P/Q-type $Ca^{2＋}$-channel and L-type $Ca^{2＋}$-channel showed localized staining (clustered pattern) on the oolemma. The distribution of the P/Q-type $Ca^{2＋}$-channel showed all localized staining, and the range of localized staining was from 1 to 8 in staining intensity. As the staining intensity increased from 1 to 8, the number of localized staining decreased. The L-type $Ca^{2＋}$-channel are homogeneously stained (29.4%-54.2%), while some of them (around 28.7%-44.1%) showed localized staining on the oolemma. However, the rest of them showed no staining at all (17.1%- 26.5%). On the contrary, the N-type $Ca^{2＋}$-channel showed mostly homogeneous staining, while nonstaining oocytes were around 33.8%. The rest showed localized staining (10%). However, staining intensity was much weaker than those of the P/Q-type and L-type $Ca^{2＋}$-channel. In fact, the N-type $Ca^{2＋}$-channel has been known to exist only in neurons (from ectoderm origin), but it is unknown how the N-type $Ca^{2＋}$-channel exists in the follicular oocytes (from mesoderm origin). Further studies are needed to examine the expression of $Ca^{2＋}$-channels during the developmental stages of the oocytes.

• Applied Microscopy
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• v.47 no.2
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• pp.63-69
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• 2017

Golgi staining has been modified and developed since Camillo Golgi introduced the black reaction in 1873. This study focuses on the commonly used Golgi staining methods and presents comprehensive data regarding three Golgi staining methods along with their strong and weak points. The Golgi-Cox method uses mercuric chloride for brain tissue impregnation and is a reliable technique for analyzing the complete dendritic tree of cortical neurons. However, specimens tend to shrink during the staining steps. Recent combination of the Golgi-Cox method and immunofluorescence provides additional options for neuroscientists. Rapid Golgi staining requires osmium tetroxide for the post-fixation process. It homogenously stains whole structures of neurons and provides their detailed anatomical morphology. This staining is influenced by the age of the specimen, temperature of the laboratory, and duration of each procedure. The Golgi-Kopsch method uses formaldehyde and glutaraldehyde instead of osmium tetroxide and can be used regardless of the age of the specimen and the duration after fixation. This method is suitable for research using human brain fixed for a long time or for specimens obtained from old-aged animals. Selecting a Golgi staining protocol that is appropriate for the specimen type and research purpose is important to achieve best results.

• Journal of Gastric Cancer
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• v.19 no.2
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• pp.173-182
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• 2019

Purpose: Intraoperative peritoneal washing cytology (PWC) is used to determine treatment strategies for gastric cancer with suspected serosal invasion. However, a standard staining method for intraoperative PWC remains to be established. We evaluated the feasibility of a rapid and simple staining method using Shorr's stain for intraoperative PWC in advanced gastric cancer. Materials and Methods: Between November 2012 and December 2014, 77 patients with clinical T3 or higher gastric cancer were enrolled. The sensitivity, specificity, and concordance between the Shorr staining method and conventional Papanicolaou (Pap) staining with carcinoembryonic antigen (CEA) immunohistochemistry (IHC) were analyzed. Results: Intraoperative PWC was performed laparoscopically in 69 patients (89.6%). The average time of the procedure was 8.3 minutes, and the average amount of aspirated fluids was 83.3 mL. The average time for Shorr staining and pathologic review was 21.0 minutes. Of the 77 patients, 16 (20.7%) had positive cytology and 7 (9.1%) showed atypical findings; sensitivity and specificity were 73.6% and 98.2% for the Shorr method, and 78.9% and 98.2% for the Pap method with CEA IHC, respectively. Concordance of diagnosis between the 2 methods was observed in 90.9% of cases (weighted ${\kappa}$ statistic=0.875) and most disagreements in diagnoses occurred in atypical findings (6/7). In overall survival, there was no significant difference in C-index between the 2 methods (0.459 in Shorr method vs. 0.458 in Pap with CEA IHC method, P=0.987). Conclusions: Shorr staining could be a rapid and reliable method for intraoperative PWC in advanced gastric cancer.

• Journal of dental hygiene science
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• v.22 no.3
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• pp.139-147
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• 2022

Background: Temporary crown resins are used prior to prosthesis placement, indicating the importance of aesthetics. The aim of this study was evaluate the color stability of various staining solutions according to the color of temporary crown resins using VITA Easyshade^Ⓡ V. Methods: The temporary crowns used were the powder-liquid type and included four shades. A total of 36 specimens were fabricated in the form of disks with a diameter of 1.8 mm and a depth of 2 mm. They were divided into four groups of nine each, and staining was performed for seven days by precipitation in 3 mL of three staining solutions composed of distilled water, black coffee, and red wine. Color and color stability evaluations were performed by a trained examiner using a digital spectrophotometer (VITA Easyshade^Ⓡ V). Color stability was analyzed using the ΔE value. Results: Because of the color stability evaluation using the ΔE value, the difference between three and seven days was significant in the specimen I and III groups (p<0.05). Further, post hoc analysis showed that the ΔE value of red wine was significant, indicating that the color stability in red wine was low. The ΔE values in group II between days three and seven were statistically significant (p<0.05). Post hoc analysis showed that distilled water, coffee, and wine had the highest ΔE values on day three. On day seven, the ΔE value for wine was significant, and the color stability was low. There was no significant difference in group IV according to the staining period and staining solution; therefore, color stability was high (p>0.05). Conclusion: This study showed that most temporary resin restorations exhibited color stability in the staining solution. The darker the color of the temporary resin restoration, the higher the color stability against extrinsic staining.

• Journal of the korean veterinary medical association
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• v.16 no.1
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• pp.25-28
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• 1980

The studies were undertaken with the objective to compare six staining techniques, nigrosineosin, eosin-nigrosin, trypan blue, Congo red-nigrosin, fast green-erythrocin and fast green-eosin and select one of these staining techniques to measure live-dead

• Journal of Environmental Science International
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• v.26 no.1
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• pp.11-21
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• 2017

In this study, bacterial growth was assessed by flow cytometry analysis of fluorescent probes-stained bacteria. Flow cytometry has many advantages of rapid analytical time, a low standard deviation, and highly sensitive detection of live and Dead E.coli over colony forming assay. When untreated bacteria were stained by using Thiazole Orange (TO) and Propidium Iodide (PI), double staining had a short analytical time as compared with that of single staining while its error rate was similar to that of single staining. Through double staining experiments, it was determined that optimal concentrations for TO and PI staining were 420 nM and $9.6{\mu}M$, respectively.

• Textile Coloration and Finishing
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• v.13 no.2
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• pp.1-1
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• 2001

In the Previous studies, we reported about the synthesis of poly[3-methylence-3-methylenesodiumbisulfite-bis(4-hydroxypheny)sulfone] (PMSBPS) and their effect on the staining of direct dye on nylon in the dyeing of nylon/cotton union. The results showed that PMSBPS have good staining resist effect on nylon in the dyeing of nylon/cotton union. In this study, the staining resist effects of PMSBPS in varied dyeing process and method were investigated, and the fastness to washing, light, and rubbing of the deed N/C unions was tested. The results are as follows ; 1. In the two bath two step method, the staining of direct dye on nylon was decreased with the concentration of PMSBPS, and the fastness to washing, light, and rubbing of the dyed N/C unions were increased by addition of PMSBPS. 2. In the one bath two step method, optimun concentration of PMSBPS was 4% 0.w.f..

• Textile Coloration and Finishing
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• v.13 no.2
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• pp.87-92
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• 2001

In the Previous studies, we reported about the synthesis of poly(3-methylence-3-methylenesodiumbisulfite-bis(4-hydroxypheny )sulfone] (PMSBPS ) and their effect on the staining of direct dye on nylon in the dyeing of nylon/cotton union. The results shoved that PMSBPS have good staining resist effect on nylon in the dyeing of nylon/cotton union. In this study, the staining resist effects of PMSBPS in varied dyeing process and method were investigated, and the fastness to washing, light, and rubbing of the deed N/C unions was tested. The results are as follows , 1. In the two bath two step method, the staining of direct dye on nylon was decreased with the concentration of PMSBPS, and the fastness to washing, light, and rubbing of the dyed N/C unions were increased by addition of PMSBPS. 2. In the one bath two step method, optimun concentration of PMSBPS was 4% 0.w.f..