• Journal of the Korean Home Economics Association
• /
• v.20 no.2
• /
• pp.45-51
• /
• 1982

Degree of separation and adhesion of dye and stain particles has been measured usually by the rate of reflection of light. However, it could be proved that the relation between the quantity of stain and the rate of reflection greatly varied with kinds of stain and states of adhesion. For this study, several pieces of cotton and polyester having different states of stain adhesion were prepared by staining them with two kinds of artificial stain different in color: Ferric Oxide and Ferric Oxynate. Every piece went through soilremoval test which employed two surfactants: Anionic LAS and Cationic M2-100. After the operation, relations between quantity of pre-soilremoval stain and rate of reflection were measured, as well as those between quantity of post-soilremoval stain and rate of reflection. Rate of reflection and quantity of stain were not proportional in measurement to the pieces stained with Ferric Oxide and Ferric Oxynate. The consequence was also the same with cotton and polyester. That held true of the fat-stained textile. With the same quantity of stain, rate of reflection varied according to the magnitude of stain particles, and the state of adhesion influenced the magnitude of stain particles a great deal.

• Korean Journal of Clinical Laboratory Science
• /
• v.39 no.3
• /
• pp.223-230
• /
• 2007

Several methods have been used in the detection of Helicobacter pylori (H. pylori) which was believed to be a pathogenic organism causing chronic gastritis, benign peptic ulcer, gastric carcinoma or malignant lymphoma. Even though several methods were introduced for detection of H. pylori in stomach, there were controversies in their sensitivities and specificities. This experiment were designed to study the comparative analysis of staining methods (hematoxylin and eosin (H&E), Giemsa, Warthin-Starry and immunohistochemical stain) to dectect H. pylori in the gastric mucosa. The results were as follows. Average density score of H. pylori classified by Genta were 2.29 in Warthin-Starry stain, 2.19 in Giemsa stain, 1.34 in immunohistochemical stain and 0.98 in H&E stain. By comparison between inflammatory degree by Sydney system and result of Warthin-Starry stain, the detection rate and densities of H. pylori were increased from mild (61.5% and 0.8), moderate (90.4 and 2.1), and severe (100% and 3.2). From the above findings, Warthin-Starry stain is useful method for detection of H. pylori in gastric mucosa.

• Archives of Craniofacial Surgery
• /
• v.9 no.1
• /
• pp.27-30
• /
• 2008

Purpose: It's difficult to treat port wine stain with nodular change in Laser therapy, and many patients with port wine stain suffer from restriction in social activity in case that port wine stain located on face. We present our experience in excision of port wine stain and skin graft in two patients who had port wine stain with nodular change on their faces. Methods: After excision of discolorized skin and nodular changed lesion of port wine stain, thick split thickness skin graft was done. Results: There was no recurred nodular hypertrophy for 3 months and 3 years of follow up periods. Conclusion: Surgical treatments of port wine stain with nodular change can lead significant improvement of lesion and social intercourse.

• Journal of Dental Rehabilitation and Applied Science
• /
• v.28 no.2
• /
• pp.213-221
• /
• 2012

The purpose of this study was to evaluate the stability of external stain that long term attrition caused by tooth brushing affected the color variation of external stain of IPS e.max Press Porcelain. The specimen was made by IPS e.max Press LT ingots, and treated the shade of external staning as Orange, A, B, C, D After conducting horizontal toothbrush strokes about 11,000 / 22,000 / 44,000 / 66,000 / 88,000, Color changers of the external stained layer was measured with SpectroShade$^{tm}$ MICRO(MHT, Italy). The result of this study was obtained as follows: 1. After 11,000 strokes, the color changes of shade were greater in Orange shade than the other 4 shade groups. And even though toothbrush strokes were increased until 88,000, there were no statistical significant color changes about the external stain shade variation. 2. The amount of color variation about increasing of toothbrushing strokes was irregular(no consistent increasing, or decreasing), and it was no statistical significant changes According to these results, the long term tooth-brushing doesn't affect the changes of external stain about IPS e.max press porcelain. And it doesn't reduce the stability of external stain clinically. Later, it will be needed to study other factors affecting the external stain.

• Journal of Embryo Transfer
• /
• v.7 no.2
• /
• pp.73-79
• /
• 1992

Effects of caffeine, heparin and caffeine-heparin treatments for in vitro capacitation of Korean Native Cattle sperm on acrosorne reaction and viability were studied using the methods of Wells-Awa and Dual stain. The results were summerized as follows: 1. The acrosome reaction of sperm when treated with caffeine after 0 to 4 hrs of preincubation were 11.0~75.7% for Wells-Awa stain, and 14.3~75.55% for Dual stain. True acrosome reaction of sperm for Dual stain was 3.0~29.2%. The viability of sperm was 62. 2~27.2%. 2. The acrosome reaction of sperm when treated with heparin after 0 to 4 hrs of preincubation were 17.0~81.2% for Wells-Awa, and 14.3~75.5% for Dual Stain. True acrosome reaction of sperm for Dual stain was 1.5~26.6%. The viability of sperm was 58.6~35. 8%. 3. The acrosome reaction of sperm when treated with caffeine-heparin after 0 to 4 hrs of preincubation were 13.0~83.2% for Wells Awa, and 11.0~78.5% for Dual stain. True acrosome reaction of for Dual stain was 5.1~26.3%. The viability of sperm was 60.5~30.1%.

• Journal of Korea Technical Association of The Pulp and Paper Industry
• /
• v.47 no.5
• /
• pp.33-42
• /
• 2015

Pitches contained in thermomechanical pulp negatively influence paper qaulity and pulp and papermaking process. Without controlling TMP pitches suitably, it is hard to make a certain quality of paper. In order to control pitches in TMP, they must be quantitatively recognized by proper tools. One of the most widely used way to detecting TMP pitches is a staining method using a hydrophobic dye such as Sudan IV. Sudan IV could be used with three different protocols including different application of its dissolution, washing, dyeing time, etc. The dyeing protocols were classified into three categories including Stain I, Stain II, and Stain III. In dyeing time, Stain I required more than 24 hours to dye pitches. On the other hand, Stain III could stain TMP pitches with the most brief way. The images of red-stained pitches could be captured by a stereomicroscope with ${\times}35$ and ${\times}45$ magnifications, and then quantitatively analysed measuring their numbers and areas by Carl Zeiss AxioVision (ver. 4.8.2) program. Among three protocols, both Stain I and Stain III were the most ideal methods to detect TMP pitches because they detected more pitches and bigger pitch areas compared to Stain II against the same specimen. In particular, it was recognized that Stain III could be used as the most useful tool to detect TMP pitches accurately within several minutes.

• Korean Journal of Clinical Laboratory Science
• /
• v.39 no.2
• /
• pp.122-127
• /
• 2007

This study is a report about a specific patient whose primary stomach adenocarcinoma metastasized to uterine cervix adenocarcinoma. A thirty-nine year old female patient was initially diagnosed as having metastatic adenocarcinoma in the supraclavicular lymph node. Upon further examination, she was diagnosed with stomach adenocarcinoma. 8 months later, a cervix punch biopsy was performed. The stains used for examination were H&E stain, PAS stain, Alcian blue stain, Mucicarmine stain, Papanicolaou's (Pap.) stain, and as immunohistochemical stains, cytokeratin 7 and 20 were done. In the H&E stain, the tumor cells showed prominent and eccentric nuclei, thin nuclear membrane in abundant mucous cytoplasm, and cylinder shape. In the PAS stain, intracytoplasmic mucin vacuoles were stained with pink, and in Alcian blue and Mucicarmine stains, intracytoplasmic mucin vacuoles were stained with blue and red. As in the above results, she was diagnosed with undifferentiated adenocarcinoma. As found on the cytologic smear preparation of the uterine cervix stained by Papanicolaou's stains, the background was relatively clear, the number of malignant cells was relatively low, and large and eccentric nuclei in abundant cytoplasm were observed. Upon observing the tissue preparation of the uterine cervix biopsy by H&E stain, a clear background, large and eccentric nuclei, and a signet ring cell types were observed, and the number of malignant cells were fewer than in the primary uterine cervix adenocarcinoma. The vacuoles in cytoplasm were observed. The nuclear membrane and chromatin were thick and very rough, and upon observation by cytokeratin 7 and 20 of immunohistochemical stain, the tumor cells indicated a positive rate of 70% and 20%, respectively. According to these results, also she was diagnosed with metastasized uterine cervix adenocarcinoma. In summary of the results of pathologic findings on stomach biopsy and cytologic, histopathologic, and immunohistochemical finding on uterine cervix biopsy, the adenocarcinoma of her uterine cervix could assert the adenocarcinoma of signet ring cell type that was metastasized from the primary undifferentiated adenocarcinoma in stomach.

• Journal of the Korean Ceramic Society
• /
• v.24 no.2
• /
• pp.155-160
• /
• 1987

The investigation includes the application of $ZrSiO_4-Cd(SxSe_{1-x})$stain to ceramic body. The currently developed stain was compared to the commercially available stain in applying to ceremic glaze. Two stains (currently developed and commecially available) were mixed with frits and applied to ceramic tiles. The tiles were fired at 850 to 120$0^{\circ}C$ and tested by colorimeter in X.Y.Z. axis of the wavelength ranging from 580nm to 650nm. Applicability 18 stains to ceramic body was investigated by ISCC-NBS of Munsell color nomenclature. $ZrSiO_4-Cd(SxSe_{1-x})$stain is as good as V and Pr series stain in mixing composition of frits 9 to 1. It maintains red color at 100$0^{\circ}C$ in the mixture of frits, and shows as the strong acid resistance stain.

• The Journal of the Korean dental association
• /
• v.15 no.8
• /
• pp.611-615
• /
• 1977

The author observed histochemically the nature of the calcified tissue in the rabbit dental pulp, induced by pulpal injection of potassium permanganate. The pulp of rabbit mandibular incisors were exposed and enlarged by a dental hand reamer. The exposed pulps were injected with 0.05ml of 20mM solution of potassium permanganate dissolved in Ringer's solution in experimental tooth. Also the control tooth received a pulpal injection of 0.05ml of Ringer's solution. After pulpal injection, the tooth was plugged with a gutta-percha root canal point. The staining techniques were hematoxylin-eosin stain, van Gieson stain, PAS reaction, toluidine blue stain, alcian blue-hematoxylin stain and colloidal iron-picric acid stain. The results were as follows: 1. The pulp on experimental tooth showed osteodentin-like calcified tissue. Also, in some areas, false denticle-like substance were observed. 2. The central portion of the calcified matrix showed metachromasia in toluidine blue stain had strong staining capacity in alcian blue stain. 3. The peripheral portion of the calcified tissue revealed marked van Gieson positive reaction for collagen. But their staining ability in alcian blue was slight and metachromasia was not appeared.

• Korean Journal of Veterinary Research
• /
• v.31 no.4
• /
• pp.501-507
• /
• 1991

Diagnosis of cryptosporidiosis is currently confirmed by the detection of the oocysts or endogenous stages in fecal or tissue samples. Various conventional staining methods and serodiagnostic techniques have been reported, but the latter has far been limited to a few laboratories. Cryptosporidium has recently been reported in mice and chiekens in Korea, but there has been no report on staining methods to the oocysts. The present study was performed by light and scanning electron microscopic observations, and discussed with staining properties of four conventional methods such as dichromate solution floatation method, Carbol fuchsin stain, Auramine-O stain and Giemsa stain method. Cryptosporidial oocysts were isolated from the laboratory mouse. In tissue sections of duodenum, jejunum, ileum, cecum and upper colon, numerous very small, basophilic bodies were observed on the border of mucosal epithelial cells. In scanning electron microscopic observations, a few of developmental stages of Cryptosporidium were seen. Two types of thick and thin-walled oocysts were recognized in the intestinal contents. Mean size of its were $5.19{\pm}0.23{\times}4.31{\pm}0.32{\mu}m$ and $5.14{\pm}0.25{\times}4.27{\pm}0.4{\mu}m$, respectively. Carbol fuchsin and Auramine-O stain methods are recommended as the satisfactory ones for the identification of Cryptosporidium oocysts. Giemsa stain was also recommended as available in the laboratory, because a few of developmental stage fo Cryptosporidium could be seen by it.