• 한국양식학회지
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• 제19권2호
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• pp.140-146
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• 2006

This study was conducted to investigate the effect of dietary oxidized oil and ${\alpha}$-tocopherol level on growth and body composition of juvenile flounder. To prepare oxidized diets, squid liver oil was oxidized by aeration at $25^{\circ}C$ for 30 days. The six diets were prepared to contain 6% fresh or oxidized squid liver oil as the lipid sources in combination with three levels of ${\alpha}$-tocopheryl acetate at 0, 80 and 800 mg/kg diet. Triplicate groups of fish ($3.9{\pm}0.1$) were fed to apparent satiation twice a day for 8 weeks. Survival was not significantly different among treatments. Weight gain, feed efficiency, daily feed intake, protein efficiency ratio and condition factor of fish fed the fresh oil diets were significantly higher than those of fish fed the oxidized oil diets (P<0.05). The increase of the vitamin E level in diets did not result in any significant improvement on growth performance of fish fed both oil diets. The vitamin E content of the liver and dorsal muscle increased with increasing dietary vitamin E level at both oil diet groups. A decreasing trend in vitamin E content of the tissues was observed in fish fed the oxidized oil diets at the same dietary vitamin E level. Significantly higher moisture content and lower crude lipid content were observed in the whole body of fish fed the oxidized oil diets than fish fed the fresh oil diets (P<0.05). Dietary lipid source affected the fatty acid content of the whole body; higher contents of saturated and monoenoic fatty acids, and lower n-3 HUFA contents such as 20:5n-3 and 22:6n-3 were observed in fish fed the oxidized oil diets than those of fish fed fresh oil diets. The results of this study suggest that the dietary oxidized oil may impair the growth performance, and an increase in ${\alpha}$-tocopheryl acetate supplementation have no beneficial effect on growth and feed efficiency of juvenile flounder.

• 한국수산과학회지
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• 제47권6호
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• pp.745-750
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• 2014

This study investigated the effects of squid Todarodes pacificus liver oil on the serum lipids and adipose tissue weight of rats. The rats in the soybean oil (SO) group received a basic diet prepared according to the recommendations of the American Institute of Nutrition (AIN-93G), with 7% SO as lipid source. The rats in the Patagonian toothfish Dissostichus eleginoides muscle oil (PTFO) group received 5% PTFO and 2% SO as the lipid source, while the rats in the eco-friendly squid liver oil (EFSO) group received 5% EFSO and 2% SO. EFSO was prepared according to the method outlined in the author's patent. PTFO was extracted from muscle using a physical method in the author's laboratory. The prominent polyunsaturated fatty acids in the dietary oils were 18:2n-6 (54.3%) and 18:3n-3 (5.64%) in SO, DHA (6.77%) and EPA (3.61%) in PTFO, and DHA (26.2%) and EPA (9.94%) in EFSO. After feeding for 4 weeks, the serum lipid levels (e.g., triglyceride, total cholesterol, and low-density lipoprotein cholesterol) of the rats in the EFSO group were significantly reduced as compared with those in the SO group (P<0.05); those of the rats in the PTFO group were also reduced, albeit not significantly, compared with the SO group. On the other hand, the total white adipose tissue (visceral, epididymal, and perirenal) weight was significantly reduced in the EFSO and PTFO groups compared to the SO group. These results demonstrate that EFSO is an effective lipid modifying agent in rat blood, and that EFSO and PTFO have greater anti-obesity effects than SO.

• 한국가금학회:학술대회논문집
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• 한국가금학회 2001년도 제18차 정기총회 및 학술발표 PROCEEDINGS
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• pp.95-96
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• 2001

This study was conducted to investigate the high-value added new products from spent hens. Spent hens were randomly designed to one of the four dietary treatments: 1) Control (commercial feed),2) T1 (commercial feed supplemented with 100 lU ${\alpha}$-tocopherol, 3) T2 (commercial feed with 10% squid liver oil and 4) T3 (commercial feed with 100 IU ${\alpha}$-tocopherol plus 10 % squid liver oil). They were fed one of the experimental diets for 15 days and slaughtered. And, also, somking of spent hens as follows : digestion in sources, boiling and somking.

• Fisheries and Aquatic Sciences
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• 제13권2호
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• pp.127-132
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• 2010

A study was conducted to investigate the effects of dietary lipid sources on the growth and fatty acid composition of juvenile sea cucumber. For 12 weeks, three replicate groups of sea cucumber (average weight 1.4 g) were fed one of three diets, containing squid liver oil (SLO), soybean oil (SO), or linseed oil (LO) as a dietary lipid source, or a control diet (CON) without added lipids. Sea cucumber survival was not significantly different among dietary treatments (P>0.05). The highest weight gain was observed in sea cucumber fed the SLO diet, whereas the weight gain of sea cucumber fed the SO diet was the lowest among dietary treatments (P<0.05). No significant differences were found in the moisture, crude protein, crude lipid, and ash contents of whole sea cucumber body among dietary treatments (P>0.05). Concentrations of 20:5n-3 and 22:6n-3 were significantly higher in sea cucumber fed the SLO diet than in those fed on the other diets. The highest 18:2n-6 and 18:3n-3 contents were observed in sea cucumber fed the SO and LO diets, respectively. The results of this study suggest that squid liver oil could be used as a good lipid source in formulated diets for juvenile sea cucumber.

• 한국식품과학회지
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• 제39권1호
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• pp.29-32
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• 2007

불포화지방산의 함량이 많은 오징어 간유의 미세캡슐화공정의 효율을 높이기 위하여 HLB 값이 다른 유화제를 이용하여 미세캡슐화 특성을 살펴본 결과, 유화액의 점도는 HLB가 낮을수록 증가하였고, 유화 안전성은 HLB 4가 가장 낮았으며 다른 실험구는 유사하게 나타났다. 오징어 간유 분말의 미세캡슐화 효율은 HLB 16으로 제조한 분말의 경우 35.0%로 높게 나타났다. 미세캡슐화된 분말의 흡습 안전성과 입도크기는 모든 실험구가 유사하게 나타나, 유화제의 HLB값에 따른 차이는 없었다. 미세캡슐화 분말의 주요 지방산 조성은 실험구간의 차이를 보이지 않았으며, 고도불포화지방산은 모든 분말이 50% 이상으로 나타났고, 포화지방산에 대한 고도불포화지방산의 비율은 HLB 11, HLB 16로 제조한 분말이 2.07로 가장 높았다.

• 한국식품저장유통학회지
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• 제14권1호
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• pp.30-34
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• 2007

불포화지방산의 함량이 많은 오징어 간유의 미세캡슐화 공정을 최적화하기 위하여 부형제 조성을 달리하여 미세캡슐화 특성을 살펴본 결과, 모든 조성비에서 유화액의 점도는 유사한 경향을 나타내었고, Na-caseinate의 함량이 높을수록 유화안정성이 증가하였다. 미세캡슐화 효율은 Na-caseinate와 cyclodextrin의 비율이 4:6 > 6:4 > 5:5 > 3:7 > 7:3의 순으로 4:6 비율이 가장 높게 나타났으나 미세캡슐화된 분말의 흡습성은 cyclodextrin의 함량이 높을수록 증가하였으며, 수분함량과 입도크기는 모든 실험구가 유사하게 나타나 큰 영향이 없었다. 지방산 조성은 모든 조성비에서 고도불포화지방산이 50% 이상으로 나타났으며, Na-caseinate와 cyclodextrin의 혼합비가 4:6으로 제조된 분말의 포화지방산에 대한 고도불포화지방산의 비율이 2.11로 높게 나타났다.

• 한국어병학회지
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• 제25권3호
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• pp.199-210
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• 2012

It can be hypothesized that dietary fatty acids can modulate immune responses in fish by inducing apoptosis of immune cells since dietary polyunsaturated fatty acid (PUFA) increase apoptosis by oxygen radicals generated by peroxidation. Thus we examined the effects of deferent dietary lipid sources such as squid liver oil (FO), linseed oil (LO) and soybean oil (SO) on oxidation (Cytochrome C oxidase; COS), apoptosis (TNF-${\alpha}$ Scinderin like) and immune (IL-$1{\beta}$ and NKEF) gene expression in the main immune organ (head kidney) in olive flounder (Paralichthys olivaceus) by Q-PCR analysis after feeding diets containing each oil (5%) for 15 weeks. Linseed oil and soybean oil were chosen to compare n-3 or n-6 enriched vegetable oils, respectively. Consequently, COS, TNF-${\alpha}$ and Scinderin like gene expression was increased in SO group, indicating the induction of oxidation and apoptosis. Meanwhile, no significant difference was found in immune gene expression. In conclusion vegetable oils containing n-3 PUFA like linseed oil seems to be more suitable lipid source than soybean oil for replacement of fish oil in flounder since n-6 PUFA in SO leads to activation of apoptosis pathways within the cellular damage in head kidney.

• 한국응용과학기술학회지
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• 제9권1호
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• pp.15-23
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• 1992

This study was aimed to observe dose-response effects of squid liver oil enriched in n3 polyunsaturated fatty acids(n-3 PUFA) on the metabolism of streptozotocint(STZ)-induced diabetic rats. In this experiment, 24 STZ-induced diabetic male rats of Sprague Dawley strain were divided into 4 groups and fed for 4 weeks with basal diet(0%). 33%,67% and 100% squid liver oil(SLO) of total fat content, and 6 normal rats were fed with 0% SLO diet at the same time. The results obtained were as follows: 1. Rat group fed with 33% SLO diet showed the least body weight loss and changes in blood glucose, while ones of 100% SLO diet showed the highest level. 2. Serum total protein and ratio of albumin to globulin of all the groups were below the standard level, but did not show significantly different among diet groups. 3. Serum creatinine concentration of all the groups were stayed whthin normal range, but BUN were 3 to 4 times higher than normal rats. BUN concentration of rats fed with 0% and 33% SLO diet was significantly lower than those of others. 4. Total-cholesterol level of serum increased in all the groups except 33% SLO diet, but since HDL-chol, levels and TG concentration went up with an incerase of SLO in the diets, the ratio of HDL-chol. to total-chol, of rats fed with 67% and 100% SLO diet showed higher than those with 0%, and 33% SLO, and TG concentration of rats fed with 67% and 100% SLO diet decreased significantly.

• 한국수산과학회지
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• 제43권5호
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• pp.445-450
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• 2010

This study investigated the effects of dietary lipid sources on growth performance and body composition of juvenile far eastern catfish, Silurus asotus. Three replicate groups of fish (average weight 3.6 g) were fed with one of the following experimental diets containing 10% beef tallow (BT), 5% BT plus 5% corn oil (CO), 5% BT plus 5% linseed oil (LO), or 5% BT plus 5% squid liver oil (SO) as the lipid source for 5 weeks. No significant difference was observed in the survival among groups. The weight gain of fish fed the LO (high in 18:3n-3) and SO (high in n-3 highly unsaturated fatty acid) diets was significantly higher than that of the fish fed the CO (high in 18:2n-6) and BT diets (P<0.05). The feed efficiency of fish fed LO and SO diets was significantly higher than that of the fish fed the BT diet (P<0.05), but not significantly different from that of the fish fed the CO diet. The protein efficiency ratio of fish fed the SO diet was significantly higher than that of fish fed the CO and BT diets (P<0.05), but not significantly different from that of fish fed the LO diet. The 18:1n-9 of whole-body polar lipid fraction in fish fed the BT diet increased compared to that of fish fed the other diets. Fish fed the CO and LO diets had significantly higher contents of 18:2n-6 and 20:4n-6, and 18:3n-3, than the fish fed the other diets in polar and non-polar lipid fractions, respectively (P<0.05). Significantly higher contents of 20:5n-3 and 22:6n-3 were observed in the whole-body polar lipid fraction of fish fed the SO diet compared with fish fed the other diets (P<0.05). The study results indicate that linseed oil and squid liver oil containing n-3 fatty acids are good dietary lipid sources for the growth of far eastern catfish.

• 한국양식학회지
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• 제6권2호
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• pp.89-105
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• 1993

n-3HUFA가 조피볼락의 성장, 영양소축적효율 및 체성분에 미치는 영향을 구명하기 위해 오징어 간유 및 대두유를 지질원으로 사료에 첨가하여 사료의 n-3HUFA 함량이 $0\~1.5\%$가 되도록 조정하여 사육한 결과는 다음과 같다. 성장, 사료효율 및 영양소 축적효율 모두 n-3HUFA 무첨가 사료에서 가장 낮은 값을 나타내었으며, 사료의 n-3HRFA 함량의 증가와 더불어 그 효과가 현저히 개선되다가 $1.2\%$ 이후에는 더이상 개선효과가 없었다. n-3HUFA함량이 높은 실험구의 간지질(비극성 지질) 함량은 감소한 경향을 보였으나 간의 glycogen 함량은 증가하였으며, HSI 값도 다소 증가하였다. 전어체의 지질함량 및 내장의 지질함량은 모두 사료의 n-3HUFA 함량이 높은 실험구에서 높게 나타났으며, 이러한 증가는 비극성 지질의 변화 경향과 일치하였다. 간 극성 지질 중의 지방산조성은 사료의 지방산조성에 따라 변화하여, 사료의 n-3HUFA가 증가함에 따라 n-3HUFA와 18:1은 증가하였고, 18:2n-6과 18:3n-3은 감소하였다.