• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.203-203
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• 2022

A new winter wheat(Triticum aestivum L.) cultivar "Hwanggeumal" was developed by the NICS(National Institute of Crop Science), RDA(Rular Dvelopment Administraion) in 2019. It was derived from a cross of the "Jokyoung//Kauz/Rayon" and "Jopoom" in 2008. It had advanced generation through bulk and pedigree method for seven years and designated line name "Jeonju398" after AYT(Advance Yield Trial) test for two years. And "Hwangeumal" was designated variety name after RYT(Regional Yield Trial) test in eight locations around Korea for two years from 2018 to 2019. Its heading date was April 19 and maturity date was May 31, which were similar to Jokyoung. "Hwanggeumal" had shorter plant height(75 cm) and spike length(7.1 cm), spikes per m²(699) and lower 1,000 grain weight(44.2 g) than "Jokyoung"(78 cm, 8.2 cm, 776, 46.6 g, respectively). "Hwanggeumal" was showed weak to winter hardiness and susceptible to powdery mildew but tolerance to PHS(Pre-harvest sprouting). The average grain yield in the AYT was 6.2 ton/ha, which were 10% more than "Jokyoung" And in the RYT was 5.1 ton/ha in upland and 4.4 ton/ha in paddy field, which were lower than "Jokyoung", respectively. "Hwanggeumal"s flour yield (71.4%) and flour lightness (91.82) showed similar to "Jokyung" and higher protein content (14.0%) and gluten content (10.3%) and SDS-sedimentation volume (60.3ml). These result showed that the "Hwanggeumal" dough strength of flour is strong than "Jokyung". "Hwanggeumal"s HMW-GS(High molecular weight gluten subunits) composition are Glu-D1 (5+10), Granule-bound starch synthase(GBSS) composition are Wx-A1 (a), Wx-B1 (b), Wx-D1 (a) and composition of Puroindolines are Pina-D1(a), Pinb-D1(b).

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.292-292
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• 2022

Since 1992, the Rural Development Administration (RDA), Republic of Korea in collaboration with International Rice Research Institute (IRRI) has developed 6 japonica rice varieties(MS11, Japonica 1, 2, 6, 7 and Cordillera 4) that are adaptable to tropical regions. However, these varieties show moderate resistance or susceptibility to certain biotic and abiotic stress. The development of varieties with more stable forms of resistance is highly desirable, and this could be possibly achieved through rapid introgression of known biotic and abiotic resistant genes. In this study, we analyzed the allele types of major biotic stress resistant genes including Xa5, Xa13, Xa21 and Xa25 for bacterial leaf blight, Pi5, Pi40, Pish and Pita2 for blast, tsv1 for rice tungro spherical virus, and Bph6, Bph9, Bph17, Bph18 and Bph32 for brown planthopper by using gene-specific molecular markers. In addition, seed quality related genes Sdr4 for preharvest sprouting and qLG-9 for seed longevity were also analyzed. The results revealed that2h5 and Xa25 resistance alleles showed in all varieties while Pi5 resistance allele showed only in MS11. The Pish resistance allele were present in five varieties except for Japonica 1. Meanwhile, for the rest of the genes, no presence of resistance alleles found in six varieties. In conclusions, most of tropical japonica varieties are lack of the major biotic stress resistant genes and seed quality genes (Sdr4 and qLG-9). Moreover, the results indicated that rapid deployment of a few major genes in the current tropical japonica rice varieties is urgent to increase durability and spectrum of biotic stress resistance and also seed dormancy/longevity which are essential traits for tropical environments.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.08a
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• pp.79-79
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• 2020

본 연구는 새싹인삼(Panax ginseng sprout) 재배용으로 이용하기 위한 묘삼(종삼)의 장기 저온저장 시 저장온도가 묘삼의 새싹인삼으로의 생장과 품질에 미치는 영향을 조사하여 묘삼의 저장에 적합한 온도를 구명하고자 수행하였다. 2년생 묘삼을 50 ㎛ LDPE 필름에 100g씩 넣어 포장하였고 온도 0, -2, -4℃에서 10개월 동안 저장하면서 2개월 마다 꺼내어 5℃ 저장고로 이동하여 5~7일 온도순화처리를 거친 후 실험을 실시하였다. 1차 육안조사로 묘삼의 부패율과 2차 생육조사로 묘삼을 재식 후 새싹인삼으로 재배 후 생장 상태와 품질을 조사한 결과 저장 4개월 이후 묘삼의 생육조사 시 새싹인삼의 생장과 품질이 묘삼의 저장온도에 따라 유의적인 차이를 보였다. 즉, 저장 4개월 된 묘삼의 건전한 새싹인삼으로의 생장은 0℃ 저장 묘삼에서 58.3%, -2℃ 저장 묘삼에서 72.1%, -4℃ 저장 묘삼에서 37.2%로 조사되었고, 저장 8개월 된 묘삼은 0℃ 저장 묘삼에서 9.7%, -2℃ 저장 묘삼에서 54.3%, -4℃ 저장 묘삼에서 6.9%로 조사되었다. 특히 0℃ 저장 묘삼은 저장 2개월에 출아가 진행되었고 저장기간이 지날수록 출아된 싹이 동해의 피해를 받는 것으로 조사되었다. 묘삼의 생육특성인 엽장, 엽폭, 경장, 근장을 측정한 결과 -2℃에 저장된 묘삼의 엽폭과 경장의 길이가 유의적으로 긴 것으로 조사되어 새싹인삼 재배를 위한 묘삼의 적정 저장온도는 -2℃로 설정하였다.

• BMB Reports
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• v.57 no.5
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• pp.244-249
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• 2024

Many types of cancer are associated with excessive angiogenesis. Anti-angiogenic treatment is an effective strategy for treating solid cancers. This study aimed to demonstrate the inhibitory effects of (E)-2-methoxy-4-(3-(4-methoxyphenyl) prop-1-en-1-yl) phenol (MMPP) in VEGFA-induced angiogenesis. The results indicated that MMPP effectively suppressed various angiogenic processes, such as cell migration, invasion, tube formation, and sprouting of new vessels in human umbilical vein endothelial cells (HUVECs) and mouse aortic ring. The inhibitory mechanism of MMPP on angiogenesis involves targeting VEGFR2. MMPP showed high binding affinity for the VEGFR2 ATP-binding domain. Additionally, MMPP improved VEGFR2 thermal stability and inhibited VEGFR2 kinase activity, suppressing the downstream VEGFR2/AKT/ERK pathway. MMPP attenuated the activation and nuclear translocation of NF-κB, and it downregulated NF-κB target genes such as VEGFA, VEGFR2, MMP2, and MMP9. Furthermore, conditioned medium from MMPP-treated breast cancer cells effectively inhibited angiogenesis in endothelial cells. These results suggested that MMPP had great promise as a novel VEGFR2 inhibitor with potent anti-angiogenic properties for cancer treatment via VEGFR2/AKT/ERK/NF-κB signaling pathway.

• Current Research on Agriculture and Life Sciences
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• v.1
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• pp.151-158
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• 1983

This study was performed to obtain a basic materials for improvement of garlic storage ability. The "Namhae jaerae" of southern strain and the "Dalsung jaerae" of northern strain were harvested by period, their stems were cut at 7cm and 25cm from disk and then placed them in blast drying system 12 hours per day at $40^{\circ}C$ for 4 days, or in natural state. The decrease rate of bulb weight was compared and rooting, sprouting, the rate of decay was investigated between natural dry(conventional method) and hot-air dry (blast drying system). The results obtained were as follows. In the case of the decrease rate of bulb weight after hot-air dry; 7cm plot" "Namhae jaerae" was similiar to one of 13th day of natural dry and 25cm plot 14th day. In "Dalsung jaerae" 7cm plot of early, common and late harvest was respectively similiar to one of 22nd, 18th and 16th day of natural dry, 25cm plot of early, common and late harvest showed the same decrease rate of bulb weight as that of 18th, 16th and 14th day of natural dry respectively. In the case of rooting and sprouting in sand culture at the early period of storage, hot-air dry showed more prolonged tendency than conventional drying method. In the case of clove state in the latter period of storage, number of eatable cloves was more numerous and number of decayed cloves were less in blast drying system than in conventional method.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.spc1
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• pp.160-165
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• 2006

Biofuctionality of soybean seeds and soy-bean products have been fortified by the uncovering of the multifuctional beneficial effects of isoflavones. As one way to fully utilize beneficial effects of isoflavones in soybean sprout is through the enhancement of isoflavone contents in soybean seeds, genetic selection for higher isoflavone and cultivational measures to increase isoflavone content in soybean seeds were attempted. Isoflavones (daidzein, gemstein) contents in soybean seeds and soybean sprouts were determined by high performance liquid chromatography. Total isoflavone contents in soybean seeds ranged from 756 to $1,682{\mu}g/g$ and Iksan #13 $(1,682{\mu}g/g)$ showed highest content among the 21 germplasms analyzed. Onetime treatment of soybean plants with Antipol or Piaster at the $V_4$ stage yielded seeds with higher isoflavones as $2,472{\mu}g/g\;or\;2,052{\mu}g/g$, respectively, which were higher by 37% and 14% than that of seeds in the control plants, respectively. In Eunhakong, Isoflavone contents of soybean sprout changed during sprouting. Daidzein content in hypocotyl increased to maximum on the third day of cultivation and decreased there-after, whereas the content changed little in cotyledon. In sprouts of Pungsannamulkong, daidzein content in hypocotyl showed a maximum level on the first day and decreased gradually thereafter but, the content changed little in cotyledon. Total isoflavone contents in lateral roots which developed on the 6th day after sprouting ranged from 4,416 to $5,232{\mu}g/g$ DW.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.65 no.4
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• pp.284-293
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• 2020

Rice consumption in Korea has been decreasing as the eating habits of the Korean people have diversified with rapid economic growth. Recently, floury endosperm rice cultivars were developed to boost rice consumption and replace wheat flour consumption with rice flour, which is vulnerable to viviparity under wet weather during the grain-filling stage because of its loosely packed starch granule structures. To overcome this limitation, it is necessary to find a suitable rice transplanting date to produce high-quality rice flour by altering the heading ecology type and changing the cultivation time by region. We examined four floury endosperm rice cultivars (FERC) in the Cheongju (central plain area) and Boeun (mid-mountainous area) regions of Korea from 2017 to 2019. Of the FERCs, the mid-late maturing types (MMT) Seolgaeng (SG), Hangaru (HGR), and Shingil (SGL) exhibited high yield and yield components after transplanting May 30 in both regions; the early maturing type (EMT) Garumi 2 (GRM2) also exhibited high yield after transplanting June 20 in Cheongju. In addition, MMTs showed the same tendency as the characteristics shown in Cheongju when grown in the Boeun region, and EMT displayed high yield and yield components after transplanting June 10. The FERCs could easily present pre-harvest sprouting in the rainy season during the grain-filling stage after 20 days post-heading because the mean temperature and frequency of more three-day rainfalls have increased over the last 5 years from the previous annual averages. Viviparity of HGR and GRM2 decreased as the transplanting date was delayed, with decreases of 2.3%-4.6% in HGR and 11.9%-23.1% in GRM2 according to the region. SGL was generally resistant to viviparity because of the Tongil type. To minimize pre-harvest sprouting and produce high yield of rice flour in the Chungbuk province, the most suitable transplanting time was the end of May in MMT and the middle and end of June in EMT.

• Restorative Dentistry and Endodontics
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• v.24 no.1
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• pp.100-115
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• 1999

The purpose of this study was to investigate the distribution of calcitonin gene-related peptide containing nerve fibers in rat pulp after dentinl injury by means of immunohistochemistry and confocal laser scanning microscope. The Spague-Dawley rats weighing about 250-300gm were used. The animals were devided into normal control and experimental groups. Experimental animals were sacrified 1, 2, 4, 7, 10, 21days after dentinal injury (dentin cutting, and then acid etching with 35% phosphoric acid) on the maxillary molar teeth. The maxillary teeth and alveolar bone were removed and immersed in the 4% paraformaldehyde in 0.1M phosphate buffer (pH 7.4), then were decalcified with 15% formic acid for 10 days. Serial frozen $50{\mu}m$ thick sections were cut on a cryostat. The rabbit CGRP antibody was used as a primary antibody with a dilution of 1:2000 in 0.01M PB. The sections were incubated for 48 hours at $4^{\circ}C$, and placed into biotinylated antirabbit Ig G as a secondary anti body with dilution of 1:200 in 0.01M PB and incubated in ABC(avidin-biotin complex). The peroxidase reaction was visualized by incubating the sections in 0.05% 3,3 diaminobenzidine tetrahydrochloride containing 0.02% $H_2O_2$. For the confocal laser scanning microscopic examination, Primary antibody reaction was same as immunoperoxidase stainning, but fluorescein isothiocyanate(FITC)-conjugate antirabbit IgG as a secondary antibody was used. The confocal laser scanning microscope was used for the examination. A series of images of optical sections was collected with a 20x objective at $3{\mu}m$ intervals throughout the depth of specimen. FITC fluerescence was registrated through a 488nm and 568nm excitation filter, and images were saved on optical disk. The stereoscopic images and three dimentionnal images were reconstructed by computer software, and then were analyzed. The results were as follows : 1. In normal control group, CGRP containing nerve fibers were coursed through the root with very little branching, and then formed a dense network of terminals in coronal pulp. 2. A slight increase in CGRP containing nerve fibers at 1 and 2day postinjury was noted subjacent to the injury site. In the 4day group, there were an extensive increase in the number of reactive fibers, followed by a partial return toward normal levels at 7~10 day postinjury, and return by 21days. 3. The sprouting of the CGRP containing nerve fibers was evident within 2day after dentinal injury, and by 4days there was a maximal increased, but was decreased at 7days and returned to normal 10~21 day postinjury. 4. In confocal laser scanning microscopic exammination, the distinct distribution pattern and sprouting reaction of CGRP containing nerve fibers were observed in stereoscopic images and three dimentional images. These results suggest that CGRP containing nerve fiber can be important role in the response to dental injury and pain regulation.

• Journal of Korean Society of Forest Science
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• v.55 no.1
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• pp.30-36
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• 1982

Wood biomass production at 1-year, 2-year, and 3-year rotations on both low and upper hills at 2m 2m spacing (25,000 trees/ha) was studied for a six-year period with following 12 species; Lespedeza cyrtobotrya Amorpha fruticosa. Robinia pseudoacacia, Acer saccharinum, Platanus orientalis Populus alba ${\times}$ P. glandulosa $F_1$, Salix alba, Pinus rigida, Alnus hirsuta var. sibirica, A. inokumai A. gultinosa, and A. incana. In One-year rotation, Lespedeza cyrtobotrya produced largest amoung of biomass (2.6 t/ha/year, fresh weight) and Populus alba ${\times}$ P. glandulosa $F_1$ the second largest (2.2 t/ha/year) on low hill. In two-year rotation, the latter produced the largest amount (4.8 t/ha/year) and Alnus hirsuta var. sibirica second largest (2.8 t/ha/year) on low hill. In three-year rotation, the largest weight (11.2 t/ha/year) was produced by Robinia pseudoacacia and the second largest (6.2 t/ha/year) by Alnus hirsuta var. sibirica on low hill Amorpha fruticosa, Acer saccharinum, Platanus orientalis and Salix alba were not suitable for biomass or fuelwood productio due to poor growth. Biomass yield on upper hill was reduced considerably for all tewlve species, with less than 4 t/year at maximum Only nitrogen fixing species (Robinia and Alnus species) are recommended on upper hill for biomass production wood sprouting ability of species was generally associated with good biomass production. Calori values of ovendry wood ranged from 4,485 cal/g for Salix alba to 5,125 cal/g for Alnus glutinosa. For maximum biomass production a three-year ratation with coppice is preferred to one-year and two-year roataions The best species appeared to be Robinia pseudoacacia and Alnus hirsuta var sibirica.

• Applied Microscopy
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• v.32 no.1
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• pp.67-80
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• 2002

This study was carried out to investigate the effects of deer antler extract on the regeneration of peripheral nerves. Sprague-Dawley male rats weighing about 300 gm were fed deer antler extract for 1, 2, and 3 weeks per oral (1.5 ml/100 gm B.W.), respectively, once a day and transected both sides of sciatic nerve of each leg. After keeping for 6 hours, sciatic nerves taken from proximal part of transected region were treated with conventional transmission electron microscopical method and then observed with electron microscope. The results obtained were summarized as follows; 1. Sciatic nerves of normal control group were not showing any sprouts and electron dense axolemmal projections were frequently observed. 2. Sciatic nerves of saline treated groups were showing axonal sprouts at the nodes of Ranvier. The length of them was usually short, and numerous vesicles, vacuoles and organelles including neurofilament were contained. The number of nodes of Ranvier containing sprouts from 100 longitudinal sectioned nerve fibers was 29 (29%) in 1 week treated group, 32 (32%) in 2 weeks treated group, and 30 (30%) in 3 weeks treated group, respectively. 3. Sciatic nerves of deer antler treated groups were showing axonal sprouts at the node of Ranvier as well. Although most of the sprouts were short, some sprouts of 2 weeks and 3 weeks treated groups were quite long. Sprouts usually contained numerous vesicles, vacuoles and cell organelles such as neurofilaments and mitochondria. The number of nodes of Ranvier containing sprouts from 100 longitudinal sectioned nerve fibers was 38 (38%) in 1 week treated group, 46 (46%) in 2 weeks treated group, and 48 (48%) in 3 weeks treated group respectively. The results described above explain pretreatment of deer antler extract improves the sprout formation of transected sciatic nerves, and then it suggests deer antler may be effective for the regeneration of peripheral nerves.