• The Korean Journal of Physiology and Pharmacology
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• v.20 no.5
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• pp.533-538
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• 2016

Angiogenesis plays an essential role in embryo development, tissue repair, inflammatory diseases, and tumor growth. In the present study, we showed that endothelial nitric oxide synthase (eNOS) regulates retinal angiogenesis. Mice that lack eNOS showed growth retardation, and retinal vessel development was significantly delayed. In addition, the number of tip cells and filopodia length were significantly reduced in mice lacking eNOS. Retinal endothelial cell proliferation was significantly blocked in mice lacking eNOS, and EMG-2-induced endothelial cell sprouting was significantly reduced in aortic vessels isolated from eNOS-deficient mice. Finally, pericyte recruitment to endothelial cells and vascular smooth muscle cell coverage to blood vessels were attenuated in mice lacking eNOS. Taken together, we suggest that the endothelial cell function and blood vessel maturation are regulated by eNOS during retinal angiogenesis.

• Korean Journal of Medicinal Crop Science
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• v.14 no.1
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• pp.19-22
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• 2006

Planting of peony, a perennial herb is usually planted in fall but the planting time can be postponed to next spring for other benefit such as land use, labour diversification and etc. This study was carried out to know the effect of planting time in spring on growth and yield of peony. Sprouting date in first year growth was earlier as planting time was later. Planting on March 10 showed best both top part and root growth and planting after that time resulted in worse growth. In planting on April 10, missing plant rate was 24% and accordingly root yield decreased to 43% comparing with that of March 10. Planting on March 10 resulted in thick and large number of root and so yield per l0a was 635 kg, the highest value among the other planting time in spring. The paeoniflorin content was not different significantly by planting time. These results suggested that March 10 was most appropriate for planting time in spring.

• Journal of Sericultural and Entomological Science
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• v.44 no.2
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• pp.45-50
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• 2002

New mulberry cultivar, Hansungppong, was bred among selected lines from F1 seedlings obtained by crossing the female, Yongcheonppong, and the male, Kugsang 21. Local adaptability test has been carried out at two places (Suwon and Chunchun) for four years since 1995. Leaf quality was tested by silkworm rearing for two silkworm rearing seasons (spring and autumn) at Suwon in 1998. Hansungppong was recognized to be superior in productivity and cold resistance to control cultivar, Yongcheonppong. The new cultivar, Hansungppong, has following agronomic characteristics as compared with Yongcheonppong, which was strongest leading cultivar against cold injury in Korea. It is belonging to Morus alba L. with the ovate leaf shape, sprouting in spring nearly the same to the Yongcheonppong (Morus alba L.), high leaf yielding of 10% compared to Yongcheonppong (Morus alba L.) and the high degree of cold resistance. It is adaptable to everywhere in Korea. Hansungppong is recommended to rear silkworms not only in spring but also in autumn besides for young silkworms.

• Journal of agriculture & life science
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• v.45 no.4
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• pp.47-58
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• 2011

The forest of Korea had been severely degraded since early 1900s until 1950s. Korean Government has successfully accomplished the reforestation works since 1960s. However, some plantations showed poor survival and growth caused by ignoring site characteristics in selecting plantation species and lack of tending works such as thinning. The natural regeneration of indigenous species, such as Quercus species and Pinus densiflora Siebold & Zucc., were examined in the plantations of Pinus koraiensis Siebold & Zucc. and Larix kaempferi Fortune ex Gordon. Quercus species regenerated mainly by sprouting while P. densiflora regenerated naturally from a few mother trees that remained in the plantations. P. koraiensis showed poor survival ($IVI{\leq}25%$) and suppressed growth (height ${\leq}3m$ and $DBH{\leq}3cm$ at 20 year-old) by Quercus species or P. densiflora in the plantation areas, however had high survival ($IVI{\geq}70%$) and growth (8 m height and 14.1 cm DBH at 20 year-old) in areas where silvicultural practices were conducted. L. kaempferi showed good survival ($IVI{\geq}40%$) and growth (17.2 m height and 16.3 cm DBH at 30 year-old) mostly in valley areas, while it was nearly dead ($IVI{\leq}10%$) in ridge or ridge-slope areas and was replaced by indigenous species such as Quercus species ($IVI{\geq}25{\sim}55%$) or P. densiflora ($IVI{\geq}18{\sim}50%$).

• Food Science and Preservation
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• v.17 no.5
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• pp.681-687
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• 2010

We examined changes in the amino acid composition of soybean sprouts of four different Korean soybean cultivars (Yutae, Jinunee, Subaktae, and Baktae). Total amino acids levels in all samples 1 day after cultivation were lower than those in soybeans but increased at cultivation times greater then 3 days. Glutamic and aspartic acids, the most abundant amino acids in all soybean cultivars studied at day 0 of sprouting, constituted more than 30% of all amino acids. Aspartic acid content of hypocotyls markedly increased, but glutamic acid levels decreased 5-day-old sprouts of Jinunee and 3-day-old sprouts of the other cultivars. Moreover, compositions of aspartic acid decreased in the order hypocotyl > root > cotyledon and Yutae > Baktae > Jinunee > Subaktae.

• Journal of Plant Biotechnology
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• v.48 no.4
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• pp.255-263
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• 2021

Paederia foetida L. is an important medicinal plant that has been used for the treatment of various gastrointestinal related ailments by different tribal communities in India. This plant is also known for its use as a food. Due to overexploitation, P. foetida has been classified as a vulnerable plant in some states of India. The propagation of P. foetida by conventional methods is easy but very slow. Synthetic seed technology offers incredible potential for in vitro propagation of threatened and commercially valuable plants, and can also facilitate the storage and exchange of axenic plant material between laboratories. However, synthetic seed production for P. foetida has not yet been reported. Thus, to the best of our knowledge, the present study is the first attempt to produce synthetic seeds of P. foetida by calcium alginate encapsulation of in vitro regenerated axenic nodal segments. Sodium alginate (3%) and CaCl₂ (100 mM) were found to be the optimal materials for the preparation of ideal synthetic seeds, both in terms of morphology and germination ability. The synthetic seeds showed the best germination (formation of both shoot as well as root; 83.3%) on ½ MS medium augmented with 0.5 mg/L indole-3-acetic acid. The plantlets obtained from these synthetic seeds could be successfully acclimatized under field conditions. We also studied the storage of these synthetic seeds at low temperature and their subsequent sprouting/germination. The seeds showed a germination rate of 63.3% even after 21 days of storage at 4 ℃; thus, they could be useful for transfer and exchange of P. foetida germplasm.

• Korean Journal of Breeding Science
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• v.43 no.2
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• pp.126-132
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• 2011

The bulb storability of eight red and ten yellow onion (Allium cepa L.) cultivars grown primarily in Korea was evaluated. During storage, sprouting occurred earlier in red cultivars than in yellow cultivars. In addition, the ratio of sprouted to unsprouted bulbs increased more rapidly in red cultivars than in yellow cultivars. However, not all yellow cultivars had strong storabilities. Bulb storability of three yellow cultivars was as poor as that of red cultivars, suggesting only a slight possibility of a pleiotropic effect of color and bulb storability. Meanwhile, $F_3$ lines of a red cultivar selected based on stronger storabilities showed intermediate storabilities between those of the red and yellow cultivars, implying that strong storability could be obtained by successive selection. In contrast, $F_1$ hybrids from crosses between yellow and red breeding lines showed poor storability compared with the yellow cultivars, indicating that poor storability may be dominant over strong storability. The relationships between color and other traits that potentially affect storability, such as fresh and dry weight, water content, and firmness of bulbs, were evaluated. No significant differences in these traits were observed between the two colors, although the water content of yellow cultivars and the dry weight of red cultivars were slightly higher than their counterparts. Correlation analyses between bulb storability and other traits, including weight, water content, and firmness, also showed no significant correlations. In this study, no correlations were identified between bulb color, storability, and other traits. However, based on these results, red cultivars with stronger storabilities could be developed through successive selection of bulbs with stronger storabilities.

• The Plant Pathology Journal
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• v.35 no.4
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• pp.372-380
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• 2019

Pseudomonas syringae pv. actinidiae (Psa) is by far the most important pathogen of kiwifruit. Sustainable expansion of the kiwifruit industry requires the use of Psa-tolerant or resistant genotypes for the breeding of tolerant cultivars. However, the resistance of most existing kiwifruit cultivars and wild genotypes is poorly understood, and suitable evaluation methods of Psa resistance in Actinidia have not been established. A unique in vitro method to evaluate Psa resistance has been developed with 18 selected Actinidia genotypes. The assay involved debarking and measuring the lesions of cane pieces inoculated with the bacterium in combination with the observation of symptoms such as callus formation, sprouting of buds, and the extent to which Psa invaded xylem. Relative Psa resistance or tolerance was divided into four categories. The division results were consistent with field observations. This is the first report of an in vitro assay capable of large-scale screening of Psa-resistance in Actinidia germplasm with high accuracy and reproducibility. The assay would considerably facilitate the breeding of Psa-resistant cultivars and provide a valuable reference and inspiration for the resistance evaluation of other plants to different pathogens.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.2
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• pp.202-213
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• 1997

In order to find out a high potential black soybean lines, of which demand has been increasing in Korea, twenty eight local cultivars were evaluated on agronomic characteristics and the classification of the cultivars was made by the cluster analysis. Days to maturity and days to growing of black soybeans were widely ranged from 58 to 82 days, and 117 to 148 days, respectively. Late maturing group showed over 140 maturity days, and heavy 100 seed weight. There was shown low yield potential in late maturing group, and growing days was positively correlated with flowering days, days to maturity, 100-seed weight and stem length, respectively. From the principal component analysis upper two components composed 76.5％ cumulative eigen value to total. Nine varietal groups were identified in relations to their affinity of the black soybeans. Selected black soybeans, Namhae-2 and Hamyang-l were field-tested and those characteristics of many pod, small seed and high yield were found out to be suitable for sprouting.

• Research in Plant Disease
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• v.28 no.4
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• pp.204-208
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• 2022

Hydrogen peroxide is an eco-friendly oxidizing agent, which has exhibited a broad spectrum of antimicrobial activity without adverse environmental impact. This study was conducted to investigate the antifungal effect of hydrogen peroxide treatment against Cylindrocarpon destructans, and consequently to evaluate its control efficacy against root rot disease of 2-year-old ginseng plants. Hydrogen peroxide treatment strongly inhibited the viability of C. destructans conidia in vitro. The hydrogen peroxide at a concentration of 300 mg/l significantly reduced disease infection of the ginseng root when treated to spore suspension (10⁷ conidia/ml). Spraying with 300 mg/l of hydrogen peroxide reduced the root rot disease of the ginseng sprouts by 15% compared to the untreated control at 14 days after the inoculation. However, 300 mg/l of hydrogen peroxide delayed the emergence of ginseng plants during sprouting under aeroponic conditions. Further works need to be done to provide an acceptable control efficacy of hydrogen peroxide against the disease and its good safety to ginseng plants.