• 한국식물병리학회지
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• 제3권2호
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• pp.120-123
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• 1987

자연상태하에서 잎도열병 병반으로부터 포자의 량을 측정하기 위하여 4종의 포자탐집기(금의 원형, 금의 개량형, 길야의 원형, 금$\cdot$길야의 개량형)를 검토한 결과, 원골한 포자형성 및 이탈, 포자채집, 검경에 있어서 한 두가지의 결점이 발견되었다. 따라서 위의 결점을 보완할 수 있는 새로운 포자채집기를 고찰, 성능을 검토하여 상기 4종의 채집기의 결점을 보충할 수 있는 결과를 얻었으므로 금후 자연상태하에서 포자이탈량 조사에 유용할 것으로 생각된다.

• Mycobiology
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• 제33권4호
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• pp.194-199
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• 2005

Application of fertilizers such as urea, diammonium phosphate (DAP) and muriate of potash in soil adversely affected the spore germination of Arthrobotrys dactyloides. Amendment of soil with urea at the concentrations of 1.0%, 0.5% and 0.1 % completely inhibited spore germination and direct trap formation on the conidium, whereas muriate of potash delayed and reduced the spore germination even at the lowest concentration. DAP also inhibited spore germination at 1.0% concentration, while at lower concentration the percentage of spore germination was reduced. Application of neem cake at the concentration of 0.5% also inhibited spore germination after 24 h of amendment. The inhibitory effect of neem cake was reduced after 15 days of amendment, while after 30 days after amendment the inhibitory effect was completely lost and the spore germinated by direct trap as in unamended soil. Nematodes were not attracted to ungerminated spores after 24 h of amendment. After 15 days of amendment nematodes were attracted to agar blocks containing fewer germinated spores after 24 h of incubation but after 48 h of incubation large number of nematodes were attracted and trapped by the germinated spores with direct traps. After 30 days of amendment, larger number of nematodes were attracted and trapped by direct traps.

• 한국토양비료학회지
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• 제49권5호
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• pp.608-613
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• 2016

Arbuscular mycorrhizal fungi (AMF) spore propagation and long term maintenance is still a complicated technique for farmers. The use of AMF for their ability to promote plant growth and protect plants against pathogen attack and environmental stresses demands AMF propagation for large scale application. This study aimed to propagate AMF spores by trap culture technique and assess their ability to propagate with different host plants in a continuous plant cycle. Mycorrhizal inoculation by trap culture in maize resulted in longer shoots and roots than sudangrass plants. Increase in dry weight with higher percentage also was observed for maize plants. After first and second plant cycle, maize plants had the higher percentage of mycorrhizal response in terms of colonization and arbuscules than sudangrass. Maximum in spore count also achieved in the pots of maize plants. The results show that maize plant is more suitable host plant for AMF spore propagation and trap culture technique can be used effectively to maintain the AMF culture for long time.

• 한국식물병리학회지
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• 제11권3호
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• pp.230-237
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• 1995

Airborne and waterborne ascospores and conidia of Botryosphaeria dothidea were collected in apple orchards at Suwon and Chunan in 1992 through 1994. Both waterborne and airborne spores were first detected in mid April to early May. Thereafter, spores were abundant in early June to late August and present until early December. Rainwater collections contained much more conidia than ascospores during the apple growing seasons. Airborne ascospores catches, which were also detected on humid days without measurable rainfall, were much more than airborne conidia catches. High amounts of ascospores were detected in early times of apple growing season, whereas most conidia catches occurred in later times of the season. The number of waterborne conidia and airborne ascospores was positively correlated with mean daily maximum, minimum, and average air temperatures during the trapping periods (p=0.01). However, no significant correlation was found between the number of spores and the total precipitation during the trapping periods.

• 한국군사과학기술학회지
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• 제7권3호
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• pp.110-121
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• 2004

Pyrolysis-mass spectrometry, incorporating an in situ thermal hydrolysis and methylation(THM) step, has been used to study biological materials for bacteria, toxin and virus. Newly developed pyrolyzer was used to decompose biological materials, and tetramethylammonium hydroxide(TMAH) was used as a methylation reagent. Chemical ionization(CI) using ethanol and ion trap mass spectrometer(ITMS) were used to ionize and analyze of pyrolysis components, respectively. Analytical characteristics of bacteria (including spore), virus and toxin were analyzed. Also acquisition and interpretation of mass spectra as biomarkers for classification/identification of biological material s were explained.

• The Plant Pathology Journal
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• 제16권3호
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• pp.147-150
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• 2000

Sporulation patterns of rice blast fungus were studied at relatively later stages of leaf blast and neck blast seasons in Icheon, Korea. This experiment was done by detaching lesion-bearing leaves and panicle bases. The number of conidia remaining on the leaf blast lesions of different cultivars from Jul 20 to Jul 23 ranged from 3,640 to 82,740 spores. More conidia were observed on the adaxial surface because they were released from abaxial surface. After heading, sporulation was observed from the lesions on the flag leaves but the number of spores was less than in the late July. Detached panicle bases or uppermost internodes infected by Pyricularia grisea produced abundant amount of conidia. Among these panicle bases, 30.1 mm size lesion recorded the highest count of 244,560 spores. When we compared the sporulation amount using the KY-type spore trap, more conidia were recorded from intact lesions than from the lesions which removed conidia and conidiophore The ratio of conidia release against total sporulation ranged from 20.5%-25.0% for leaf blast and 8.2%-25.3% in the neck blast. Effective inoculum potential was also discussed.

• 한국대기환경학회지
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• 제29권6호
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• pp.780-788
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• 2013

This study attempted to determine important meteorological parameters related to airborne pollen concentrations in urban areas. Hourly pollen measurement data were prepared from a regular sampling with a volumetric Burkard spore trap at a site in the Ulsan city, during the spring season (March~May) of 2011. Results showed that the daily mean and maximum concentrations for total pollen counts during the spring season were statistically significantly correlated with both air temperature and wind speed; daily mean pollen concentration was the most highly related to daily maximum temperature (r=0.567, p<0.001). It was also identified that pollen concentration has a stronger relationship with wind speed at the rural site than at the urban one, which confirms that strong wind conditions over the pollen sources area can be favorable for pollen dispersal, resulting in increases in airborne pollen concentrations downwind. From the results of an oak-pollen episode analysis, it was found that there was a significant relationship between hourly variation of oak pollen concentrations and dynamic meteorological factors, such as wind and mixing height (representing the boundary layer depth); especially, a strong southwestern wind and elevated mixing height was associated with high nocturnal concentrations of oak pollen. This study suggests that temperature, wind, and mixing height can be important considerations in explaining the pollen concentration variations. Additional examination of complex interactions of multiple meteorological parameters affecting pollen behavior should be carried out in order to better understand and predict the temporal and spatial pollen distribution in urban areas.