• Journal of Applied Biological Chemistry
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• v.55 no.4
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• pp.273-275
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• 2012

The attraction effects of light emitting diode (LED) trap to Spodoptera exigua adults were evaluated in greenhouse and compared with those of no light trap and black-light trap, which is typical used in commercial trap. 7 days later, white LED trap ($83.0{\pm}0.7$) was 2.4 times more attractive than black-light trap ($34.0{\pm}2.6$), whereas the no light trap was little attractive to S. exigua. These results suggest that white LED traps could be used for environmental insect control.

• Korean journal of applied entomology
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• v.39 no.3
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• pp.199-205
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• 2000

The field control efficacy of entomopathogenic nematode, Steinernema carpocapsae Weiser, was evaluated on the beet armyworm, Spodoptera exigua (Hiibner). The insect pest has been known to be a defoliator at the aerial part of the crop and difficult to be controlled effectively with most commercial chemical insecticides due to its insecticide resistance. To overcome the susceptibility of the nematodes to desiccation when they were applied by leaf spray in field condition, we screened several commercial antidesiccants (alkyl glucoside, CMC, glycerol, Keltrol-F, Kunipia-G, and Laponite LXG) optimal for survival of the nematodes. Keltrol-F (0.1 %) was selected as a candidate supplement for field application of the nematodes. Leaf spray of the nematodes at 5,000 infective juveniles/ml of distilled water containing 0.1 % Keltrol-F resulted in 87.7% control efficacy on the 3rd instar larvae of Sp. exigua.

• Proceedings of the Korean Society of Applied Entomolohy Conference
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• 2005.05a
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• pp.107-107
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• 2005

• Korean journal of applied entomology
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• v.34 no.3
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• pp.169-173
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• 1995

This study was conducted to investigate control effects against Spodoptera exigua Hubner in field using mating disruption dispensers of synthetic sex pheromone during 1993 and 1994. Denisities in egg and larva of Spodoptera exigua in field treated with the dispensers was 1.9% level of that without dispensers in welsh onion, 12.5% in red pepper and 4.4% in potato in 1993. Also 5.9% and 5.2% were counted from 10 ha and 20 ha of welsh onion field compared. Control value in Fields with dispensers were maintained at 80% level in 10 ha and 90% level in 20 ha until October.

• Korean journal of applied entomology
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• v.30 no.3
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• pp.212-218
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• 1991

Pathogenicity of Spodoptera exigua nuclear polyhedrosis virus (SeMNPV) against the host insect and 8 species of lepidopterous insects and cross infection of baculoviruses to third instar of S. exigua larvae were studied to determine as a biocontrol agent for S. exigua. The median lethal concentrations($LC_{50}$)of the SeMNPV to egg mass was $2.855\times10^5$ PIBs/ml and higher than that to the larvae of S. exigua. Mortality of the SeMNPV in third ins tar larvae was more increased than that in first and fifth instar of S. exigua larvae by 1.16 and 4.11 times, respectively. The median lethal times($LT_{50}$) to $1.56\times10^6$ PIBs/ml was in the range of 4.25 to 5.04 days. Infectivity of the SeMNPV against eight species of lepidopterous insects was showed only in the host insect, S. exigua. Autographa cali/ornica MNPV, Mamestra barassicae MNPV, and Trichoplusia ni MNPV were cross-infected to third instar of S. exigua larvae among ten of baculoviruses tested.

• Korean journal of applied entomology
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• v.37 no.1
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• pp.23-30
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• 1998

Restriction fragment length polymorphism (RFLP) of a DNA has been a useful tool for analyzing genetic variation. This research was performed to establish an RFLP analytic method on the mitochondrial DNA (mtDNA) of the beet armyworm, Spodoptera exigua (Hiibner). To do this, total size of the mtDNA was measured and polymerase chain reaction (PCR) primers were selected. Its mitochondrial genome size was ca. 16kb. From a serial PCR test of 29 primers refered to the compilation of Simon et al. (1994), 22 primers were selected to amplify its mtDNA fragments. These primers resulted in short (300-700 bp) or long (1000-2000 bp) DNA products which represented a total or partial sequence of each of CO-I, CO-11, Cyt-B, ND-1, 12s rRNA, 16s rRNA, and some tRNAs. PCR-RFLP was performed in some variable mtDNA regions with 8 kinds of 4bp recognizing restriction enzymes. Different populations from Andong, Kyungsan, and Sunchun did not show any restriction site polymorphisms but had some length variation in certain regions of mtDNA.

• Korean journal of applied entomology
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• v.37 no.2
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• pp.163-170
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• 1998

Larval morphological characters such as body color and stripe pattern were analyzed to get morphological genetic markers of beet armyworm, Spodoptera exigua (Hiibner). Body color was varied from light green to dark brown with diets. Stripe characters were classified by the presence of dorsal and lateral lines: three stripes with both lines, one stripe with dorsal line only, and zero stripe. Proportions of each stripe character increased with successive selections for its own character. Three stripe was dominant to one stripe when they were crossed. The estimated heritability in narrow sense (h2) of the stripe pattern was 0.50k0.42. About two fold more females than males were produced in zero stripe line. Stripe pattern was not significantly changed by different diets except in welsh onion which had lower proportion of three stripe individuals than that of the expected. Larval stripe pattern was also correlated with larval and pupal developmental rates and cold hardiness but not with insecticide susceptibility.

• Korean journal of applied entomology
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• v.30 no.2
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• pp.144-149
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• 1991

Biochemical characteristics of Spodoptera uigua nuclear polyhedrosis virus (SeNPV) isolated in Jinju were studied. SeNPV contained a number of nucleocapsids within a viral envelope embeded in polyhedra. The polyhedral protein of SeNPV was composed of a single polypeptide with a M. W. of 30kd. Double-immunodiffusion test showed that the polyhedral protein of SeNPV had common antigenic determinants with SINPV and BmNPV. Virion proteins of SeNPV were resolved into 49 polypeptides by silver staining after SDS-PAGE. The approximate genome size of SeNPV by restriction endonuclease analysis was 1l0kb.

• Korean journal of applied entomology
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• v.36 no.3
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• pp.277-282
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• 1997

Chemosterilant effect of chlorpyrifos-methyl (CPM) on the beet armyworm, Spodoptera e.rigua (Hubner) was investigated, using topical application. Egg fecundity and fertility were analyzed in the adults reared from the fifth instar larvae which were treated with sublethal doses of CPM. Though CPM reduced fecundity a little, it gave significant negative effect on egg fertility in a dose-dependent manner. Genetic analysis was performed by reciprocal crosses between treated (100 Wgllarva) and untreated individuals and showed a dominant lethal effect of CPM on egg fecundity and fertility. This results indicate that chlorpyrifos-methyl may act as a chemosterilant as well as a well-known neurotoxicant, and suggest that it can be used in genetic control program.

• Korean journal of applied entomology
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• v.29 no.3
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• pp.180-183
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• 1990

Simple mass-rearing methods for Spodoptera exigua Hubner with an artificial diet were done in th laboratory. Hatchability of egg and its survival rates upto 3rd instar lava were 97.9 and 83.3 ercent, respectively. The pupation rates in individual rearing, mass-rearing, mass-rearing with sawdust were 48.5%, 37.5%, and 82.5%, respectively. The emergence rates in those methods were 85.2%, 86.7%, 90.9%, respectively.