• KOREAN JOURNAL OF CROP SCIENCE
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• v.45 no.4
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• pp.237-240
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• 2000

This experiment was conducted to evaluate the effect of split application of nitrogen(N) on development of vascular bundle(VB) and yield components of rice. Two cultivars were used in this study; IR58, an indica type and Shinunbongbyeim a japonica type. The number and total cross sectional area of the VB in the peduncle and leaf blade were more and bigger in N split application than 100 percent basal fertilizer. Nitrogen split application at necknode differentiation stage increased the number and size of the VB. Nitrogen split application resulted in increased panicle number with application of N before transplanting and at tillering stage; increased spikelets number with N application at necknode differentiation stage; and increased spikelet fertility and 1000 grain weight with N application at necknode differentiation and heading stages. Grain yield increased 7-10% in N split as compared to all basal application. The total cross sectional area of VB in peduncle closely correlated with the number of spikelets per panicle. Nitrogen management can have an impact on spikelet differentiation through more and bigger VB and increase grain yield potential.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.27 no.2
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• pp.116-122
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• 1982

In three diverse wheat and one barley cultivars were used to examine the relation of the spikelet and floret differentiation to fertility and an winter wheat Norin 61 was used to examine the spikelet size gradients in the mature ear in relation to their grain development. The greatest number of grains and florets per spikelet, and the heaviest grains occurred in the low-mid part of the ear. Within the spikelets, the 2nd grain from the base was heavier than the I st or the 3rd one. In this region, the fertility of the 1 st floret was 97%, the 2nd 93%, the 3rd 88%, and 4th floret was 15%. The fertility of the wheat and barley was closely related to spikelet and floret differentiation. Barley spikelets was fertile as their differentiation stage was X that is 25 days before heading. Wheat florets were fertile as their differentiation stage was X that is 15-20 days before heading. But, they were sterile or reduced in fertility if they were not reached to the stage X.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.48 no.3
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• pp.252-261
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• 2003

Although the young spike of barley (Hordeum vulgare L.) or wheat (Triticum aestivum L.) is known as the most susceptible part to spring cold injury, the risk of cold injury is apt to be ignored in most breeding program due to the importance of early maturity. Based on these aspects, the types and inducing time, temperature conditions for induction and effects of cold injury on growth and yield in this study were investigated under greenhouse and field conditions through three years (1997-1999). In natural condition, low temperature around -2.4∼$-10.2^{\circ}C$ caused the death of plant. Several cold injury types such as partial degeneration of spike, partial discoloration of leaf, spike and awn, discoloration of culm and white spike were observed at low temperature around $-3.1^{\circ}C$. Low temperature around -2.4∼$-8.6^{\circ}C$ and 1.3-$7.6^{\circ}C$ caused degeneration and sterility of spike, respectively. Most materials were prepared to the spikelet foundation stage, spikelet differentiation stage, development stage of flower organ, booting stage and heading stage, which were known having risk for cold injury in field condition. Although most of the controlled stages were sensitive to the induced low temperature, booting stage was the most sensitive stage for cold injury. All of growth stages which were treated-heading stage, booting stage, development stage of flower organ, spikelet differentiation stage, spikelet foundation stage-were responded to low temperature treatment but the symptoms revealed were very specific according to the growth stages. Ears of plant in heading stage were discolored to white. Ears of plant in booting stage were degenerated in all or part of one. Plants in spikelet differentiation stage were sterile in all or part of one. When tried to detect the specific differences between normal and cold injured plants in appearance, spike length, distance between spike and flag leaf and the first internode length could be the critical points for occurrence of spike death caused by cold injury. In barley, the elongation of spike was stopped on 3.2cm after occurrence of spike degeneration, 4.7cm after occurrence of partial degeneration of spike, 5.0cm after occurrence of white spike. In wheat, it was stopped on 1.6cm after occurrence of stem death, 3.3cm after occurrence of spike degeneration, 8.3cm after occurrence of partial degeneration of spike, 8.1cm after occurrence of white spike, 7.5cm after partial discoloration of leaf and 9.3cm after partial discoloration of spike. The obtained results from low temperature treatment induced in growth chamber were similar to the field experiment, Beacuse the death of spikes was more when low temperature was treated two times than one times, the temperature should be upgrade to -3$^{\circ}C$ in order to get the same condition with field test.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.5
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• pp.503-514
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• 1997

The morphogenesis of panicle and spikelet in paddy rice has been studied in high yielding Indica$\times$Japonica hybrid cultivar, Milyang 23 and a Japonica type cultivar, Koshihikari. Germinated seeds planted in $5000^{-1}$ a pots filled with submerged soil and cultured under natural conditions. The young panicle of main stem were continuously dissected and observered by Cryo-SEM from the panicle initiation stage until heading stage. Although the date of panicle differentiation and heading in Koshihikari earlier than those of Milyang 23. the sequence of panicle development in two cultivars begins when first bract primordium at opposite side of flag-leaf primordium differentiated, synchronously followed by growth of the primary branch primordia (PBPs) and secondary branch primordia (SBPs), spikelet primordia(SPs), glumes as lateral organs on rachilla and organs composing single floret, and successive sporogenesis in the young spikelets continue after the enclosure by lemma and palea. The PBPs are acropetally initiated from the base of the panicle primordium, and the SBPs alternately differentiate from the base of upper PBP which differentiate later than the lower PBP. Spikelet development starts at the top of upper side PBP of the young panicle and continue basipetally even though SBPs continue to develop at the lower primary branch. Each PBP, SBP and SP differentiate with differentiation bract or bract hair cell around the base of each their primordia. The observation could confirm that Milyang 23 has not only 2~3 more defferentiated PBPs, but also more SBPs and SPs especially from middle-lower primary branch, at end of their differentiation stages, as compared to those of Koshihikari.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.36 no.5
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• pp.394-406
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• 1991

It was proved that cold tolerance of rice plants at the young microspore stage was affected by water temperature and nitrogen application from the spikelet differentiation stage to the young microspore stage, and this mechanism could be explained in the point of view of pollen developmental physiology. The cold tolerance of rice plants at the young microspore stage was severely affected by water temperature (Previous water temperature) and nitrogen application(Previous nitrogen application) from the spikelet differentiation stage to the spikelet differentiation stage. Although the duration is only 10 days or so from the spikelet differentiation stage to the young microspore stage, these days are very important period to confirm the cold tolerance of rice plants at the young microspore stage. The higher previous water temperature up to $25^{\circ}C$ and the deeper previous water depth up to 10cm caused the more cold tolerance of rice plants. Water irrigation of 10cm before the cretical stage showed lower cool injury than that of water irrigation of 20cm during the critical stage. The preventive effect of cool injury by combined treatment of the deep water irrigation before and during the critical stage was not additive but synergistic. The cold tolerance of rice plants grown in previous heavy nitrogen level was rapidly decreased when nitrogen content of leaf blade at the young microspore stage was excessive over the critical nitrogen level. Nitrogen content of leaf blade at the changing point of cold tolerance was estimated as about 3.5% for Japonica cultivars and about 2.5% for Indica x Japonica cultuvars. It is considered that these critical nitrogen contents of leaf blade can be used as a index of the safe critical nitrogen level for the preventive practices to cool injury. It was summarized that increase of engorged pollens per anther by high previous water temperature resulted from the increase of number of differentiated microspores per anther, otherwise, the increase of engorged pollens by the decrease of previous nitrogen level was caused by the decrease of the number of aborted microspores per anther.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.6
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• pp.833-840
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• 1997

Differentiation and degeneration of spikelets in paddy rice has been studied in high yielding Indica$\times$Japonica hybrid cultivar, Milyang 23 and a Japonica type cultivar, Koshihikari. Germinated seeds planted in 5000$^{-1}$ a pots filled with submerged soil and cultured under natural conditions. The young panicles of main stem were continuously dissected and observered by Cryo-SEM from the panicle initiation stage, and investigated about formation position of the differentiation and degeneration spikelet within a panicle of 7 days after heading. The degeneration of spikelet appeared simultaneously throughout panicle just after closure of spikelet by the palea and lemma. Differentiated and degenerated spikelets per panicle were about 240, 80 for Milyang 23 and 87, 6 for Koshihikari, respectively. The spikelets degeneration in Milyang 23 was mainly on the secondary and tertiary branch which were developed from primary branch of middle-basal panicle node and hardly not the spikelets of primary branch, and degeneration rate of secondary and tertiary rachis branch and spikelets for Milyang 23 were 2.5 times greater than those of Koshihikari. The proper relation equation between total differentiation or normal spikelets number per panicle(Y) and each rachis branch number were different between cultivars, Le., Y=5.5X$_1$＋3.0X$_2$ for Koshihikari as previously proposed, but those of Milyang 23, Y=5.7X$_1$＋3.5X$_2$＋2.8X$_3$ for total differentiation spikelets and Y=5.6X$_1$＋3.2X$_2$＋2.4X$_3$ for normally developed spikelets, where X$_1$, X$_2$, X$_3$ are number of primary, secondary, tertiary rachis branch, respectively.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.30 no.4
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• pp.359-367
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• 1985

This experiment was conducted to study effect of cold water damage on some growth characters related to source and sink at reproductive growth stage in Jinan (sea level 303m). The cold water irrigation duration had irrigated 4, 8 and 12 days at panicle formation stage and reproduction division stage compared to perennial water irrigation. Cold water irrigation shortened culm length and panicle length and degree of panicle exsertion. The shortening effect appeared great at lower internodes when treated at panicle formation stage but at higher internodes when treated at reduction division stage. Cold water irrigation decreased the number of secondary branches and spikelets per panicle, and increased the number of degenerated spikelets being high degeneration when treated at panicle formation stage. Spikelet sterility and impediment of grain filling were affected by duration of cold water irrigation being great when treated at spikelet primodium differentiation stage and reduction division stage in particular. Grain weight was also reduced. Significant relationship existed between spikelets sterility, grain filling and yield. The degeneration of secondary branches and spikelets correlated with leaf area but spikelet sterility and yield with culm length, panicle length and panicle exsertion.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.30 no.1
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• pp.84-92
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• 1985

This experiment was carried out to study the morphological traits, yield and yield components of barley cultivars - Kangbori, Olbori and Suwon 18 - on different seeding dates. It appeared generally rapid development of young spike from late stage of floret differentiation (X stage) to complete it at heading stage. Young spike appeared more influencials by the different seeding dates rather than years and cultivars. Stem started the development from late stage of spikelet differentiation (VII stage) to early stage of floret differentiation (IX stage), indicating the continuing development from basal to upper internodes. Number of spikes per unit area showed more effect in seeding date rather than cultivar and year. However, number of kernels per spike and 1,000 kernel weight affected more due to years rather than cultivar and seeding date. It was significantly difference in grain yield according to different seeding dates.

• Journal of Plant Biology
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• v.20 no.3
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• pp.127-134
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• 1977

In order to investigate the changes of free amino acids during the differentiation and development of young spike in naked barley, a typical spring grain, Wanju, and two winter grains, Sedohadaka and Nonsankwa No. 1-6 differing in their spring habits, were analyzed at different growth stages by thin layer chromatography. In all the varieties 22 ninhydrin positive components were detected at the sowing time of March 5 and 20 components in the sowing plots of March 30. In case of the latter plot, β-alanine was identified only in both Wanju and Sedohadaka, whereas pipecolic acid was detected only in Nonsankwa No. 1-6. Particularly, it is interesting that β-alanine was observed only in the case showing the normal heading independent of the varieties and sowing times. Whether these components are directly related to the physiology of spring habits in barley or not is also a question to be answered. Of the major amino acids, alanine and γ-aminobutyric acid were always detected in appreciably large spots, and serine, leucine, aspartic acid, valine and asparagine were somewhat larger. In the plot of march 30, glutamic acid was also detected in very large spot in both Wanju and Sedohadaka at the stage of spikelet differentiation and in Nonsankwa No. 1-6 at the stage of bract differentiation. Histidine, which showed the only qualitative difference among the varieties during seed germination, cannot be observed at all. Proline observed considerably large spot during seed germination was always detected but very small except that it was observed in large spot at the stage of floret differentiation in Nonsankwa No. 1-6 in the plot of March 5.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.58 no.4
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• pp.353-361
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• 2013

The time of panicle initiation change by transplanting date, and this change is affected by heading ecotype and seedling age. So we assessed the variations of panicle initiation, spikelet differentiation and heading date affected by transplanting dates, rice cultivars and seedling ages. And we compared the growth durations and meterological factors between chief growth stages. The differences of growth duration from transplanting date to spikelet differentiation by seedling age were 1~3 days in all transplanting of Unkwang, but it increased to 4 days in Hwayeong transplanting on May 1 and June 30, and Nampyeong transplanting on June 30. The growth durations from panicle initiation to heading of Unkwang and Hwayeong increased until transplanting time by May 31, and decreased thereafter. The growth durations of Nampyeong increased in transplanting on May 16 and May 31. In each transplanting, mean temperature of 30 days after heading was highest in early transplanting, but sunshine hours in the period were highest in transplanting on June 30 in Unkwang, in transplanting on June 15 in Hwayeong, and higher in transplanting on May 31 and June 15 in Nampyeong. The growth duration between spikelet differentiation and heading showed variation according to rice cultivars and transplanting date, Those were 22~26 days in Unkwang, 21~27 days in Hwayeong and 21~28 days in Nampyeong.