• 한국작물학회지
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• 제47권3호
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• pp.236-239
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• 2002

한국산 맥문동과 중국산 소엽맥문동의 성분패턴비교에서 HPLC 분석 결과, 중국산 소엽맥문동(Ophiopogon japonicus)은 RT. 21.2분의 peak는 표준품과 대조결과 spicatoside B인 것으로 확인되었다 반면에 국산 맥문동(Liriope platyphylla)은 spicatoside A와 spicatoside B가 표준품 대조 결과 RT. 6.8분과 21.2분대에서 확인되었다. 따라서 건조된 상태에서 혼용되어 사용하고 있는 한국산 맥문동과 중국산 소엽맥문동은 HPLC 성분패턴상으로 감별이 가능하게 되었다.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.1
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• pp.234.1-234.1
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• 2003

Spicatoside A and spicatoside B were isolated from Liriopis tuber. Spicatoside A has anticancer activity and it is composed of 1 ${\beta}$-hydroxy diosgenin and trisaccharides. Spicatoside B has the preventive effect of diabetes mellitus and also has 1 ${\beta}$-hydroxy derivatives, which is clevaged the ether linkage of F ring of diosgenin. Therefore, selective synthesis of 1 ${\beta}$-hydroxydiosgenin is required. (omitted)

• Biomolecules & Therapeutics
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• 제24권5호
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• pp.469-474
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• 2016

Liriopogons (Liriope and Opiopogon) species are used as a main medicinal ingredient in several Asian countries. The Liriopes Radix (tuber, root of Liriope platyphylla) has to be a promising candidate due to their source of phytochemicals. Steroidal saponins and their glycosides, phenolic compounds, secondary metabolites are considered of active constituents in Liriopes Radix. Spicatoside A, a steroidal saponin, could be more efficacious drug candidate in future. In this review, we summarized the available knowledge on phytochemical and pharmacological activities for spicatoside A. It significantly suppressed the level of NF-${\kappa}B$, NO, iNOS, Cox-2, IL-$1{\beta}$, IL-6 and MAPKs in LPS-stimulated inflammation. The production of MUC5AC mucin was increased. MMP-13 expression was down-regulated in IL-$1{\beta}$-treated cells and reduced glycosaminoglycan release from IL-$1{\alpha}$-treated cells. The neurite outgrowth activity, PI3K, Akt, ERK1/2, TrkA and CREB phosphorylation and neurotropic factors such as NGF and BDNF were upregulated with increased latency time. It also showed cell growth inhibitory activity on various carcinoma cells. From this, spicatoside A exerts anti-inflammation, anti-asthma, anti-osteoclastogenesis, neurite outgrowth, memory consolidation and anticancer activities. Further studies are needed on spicatoside A in order to understand mechanisms of action to treat various human diseases.

• 대한본초학회지
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• 제25권4호
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• pp.95-102
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• 2010

Objectives : Specifications by Processing Methods of Liriope platyphylla (LP) was not established in korea. We carried out method study for manufacture of LP. Above all, LP were purchased and were processed at four kinds. First group had hearts of swelling roots, not cut bodies(LP-A). Second group had not hearts of swelling roots, not cut bodies(LP-B). Third group had hearts of swelling roots, cut bodies(LP-C). Forth group had an aggregate of hearts of swelling roots(LP-D). Methods : We has gained losses on drying, contents of ethanol-soluble extracts, contents of water-soluble extracts and contents of spicatoside A. Results : 1. Losses on drying of all group were less than 18% that is a standard of chinese pharmacopoeia. 2. Contents of ethanol-soluble extracts at LP-C, LP-D were included more than contents of those in the other groups in significance levels. 3. Contents of water-soluble extracts at LP-A, LP-C were included more than contents of those in the other groups in significance levels. 4. Contents of spicatoside A in LP-B were included more than content of spicatoside A in the other groups in significance levels. Resultingly, standards of LP in korea were proposed compatible proposals suggested as below. 1. Losses on drying of LP were less than 15% that is less than that of chinese pharmacopoeia. 2. Form to boil in water must be cutting body with heart of swelling root. 3. Because there is no content of spicatoside A in heart of swelling root, it is not suited to purpose a index component. Conclusions : The subjects of loss on drying and form of LP to boil in water must be contained in korean pharmacopoeia. Moreover, because of much valuable LP in food and medicine, it is urgently required aspect of index component such as spicatoside B, spicatoside C, flavonoid, polysaccharide having medical actions and so on.

• 생약학회지
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• 제22권2호
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• pp.73-77
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• 1991

Further studies have been done on the constituents of the tubers of Liriope spicata $L_{OUR}$ (Liliaceae). Four steroidal glycosides, tentatively designated as compounds I. II, III and IV, were isolated from the n-BuOH soluble fraction of this plant. The structures of these glycosides were established as ${\beta}-sitosterol$ glucoside, prosapogenin II of spicatoside A, ophiopogonin B, and prosapogenin III of spicatoside A.

• Archives of Pharmacal Research
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• 제12권4호
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• pp.295-299
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• 1989

Two novel steroidal saponins designated as spicatosides A(1) and B(2) were isolated from the tubers of Liriope spicata and their structures were elucidated as 25(S)-rus-cogenin -1-0- fJ - D-glucopyranosyl (1$\rightarrow$2)- [$\beta$ - D-xylopyranosyl (1$\rightarrow$3)] -$\beta$- D- fucopyranoside (1) and 26-0-$\beta$-D-glucopyranosyI25(S)-22-0-methyl-furost-5-en-l$\beta$, 3$\beta$, 26-trioll-0-fJ -D¬glucopyranosyl (1$\rightarrow$2)- [$\beta$- D-xylopyranosyl (1$\rightarrow$3)] - $\beta$- D- fucopyranoside (2), respectively.

• Applied Biological Chemistry
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• 제41권5호
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• pp.390-394
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• 1998

맥문동 괴근을 80% MeOH 수용액으로 추출하고, 얻어진 추출물을 EtOAc, n-BuOH 및 $H_2O$로 용매 분획하였다. 암세포 A549, SK-OV-3, SK-Mel-2, XF-498 및 HCT-15에 대한 각 분획물의 생장억제활성을 SRB 법으로 측정한 결과 n-BuOH 분획에서 암세포 생장억제활성이 높게 나타났다. n-BuOH 분획으로부터 Amberlite XAD-II 및 silica gel column chromatography를 반복하여 2종의 사포닌을 분리하였고, NMR, IR 데이터의 해석과 가수분해반응을 이용하여 spicatoside A와 B로 동정하였다. Spicatoside A가 암세포에 대한 생장억제활성을 보여 A549, SK-OV-3, SK-Mel-2, XF-498 및 HCT-15에 대한 $IC_{50}$ 값이 각각 17.3, 21.7, 14.9, 18.8 및 $15.6\;{\mu}g/ml$를 나타내었다.