• Journal of Microbiology
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• v.43 no.2
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• pp.172-178
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• 2005

To determine the prevalence and genotypes of $\beta$-lactamases among clones of a metagenomic library from the cold-seep sediments of Edison seamount (10,000 years old), we performed pulse-field gel electrophoresis, antibiotic susceptibility testing, pI determination, and DNA sequencing analysis. Among the 8,823 clones of the library, thirty clones produced $\beta$-lactamases and had high levels of genetic diversity. Consistent with minimum inhibitory concentration patterns, we found that five ($167\%$) of thirty clones produced an extended-spectrum $\beta$-lactamase. 837- and 259-bp fragments specific to bla$_{TEM}$ genes were amplified, as determined by banding patterns of PCR amplification with designed primers. TEM­1 was the most prevalent $\beta$-lactamase and conferred resistance to ampicillin, piperacillin, and cephalothin. TEM-116 had a spectrum that was extended to ceftazidime, cefotaxime, and aztreonam. The resistance levels conferred by the pre-antibiotic era alleles of TEM-type $\beta$-lactamases were essentially the same as the resistance levels conferred by the TEM-type alleles which had been isolated from clinically resistant strains of bacteria of the antibiotic era. Our first report on TEM-type $\beta$-lactamases of the pre-antibiotic era indicates that TEM-type $\beta$-lactamases paint a picture in which most of the diversity of the enzymes may not be the result of recent evolution, but that of ancient evolution.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• 2006.10a
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• pp.134-143
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• 2006

Out of all types of motions the critical motions leading to capsize is roll. The dynamic amplification in case of roll motion may be large for ships as roll natural frequency generally falls within the frequency range of wave energy spectrum typical used for estimation of motion spectrum. Roll motion is highly non-linear in nature. Den are various representations of non-linear damping and restoring available in literature. In this paper an uncoupled non-linear roll equations with three representation of damping and cubic restoring term is solved using a perturbation technique. Damping moment representations are linear plus quadratic velocity damping, angle dependant damping and linear plus cubic velocity dependant damping. Numerical value of linear damping coefficient is almost same for all types but non-linear damping is different. Linear and non-linear damping coefficients are obtained form free roll decay tests. External rolling moment is assumed as deterministic with sinusoidal form. Maximum roll amplitude of non-linear roll equation with various representations of damping is calculated using analytical procedure and compared with experimental results, which are obtained form forced tests in regular waves by varying frequency with three wave heights. Experiments indicate influence of non-linearity at resonance frequency. Both experiment and analytical results indicates increase in maximum roll amplitude with wave slope at resonance. Analytical results are compared with experiment results which indicate maximum roll amplitude analytically obtained with angle dependent and cubic velocity damping are equal and difference from experiments with these damping are less compared to non-linear equation with quadratic velocity damping.

• Proceedings of the Earthquake Engineering Society of Korea Conference
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• 2002.03a
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• pp.3-11
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• 2002

Source parameters for forty nine recent earthquakes occurred in and around Korean Peninsula are determined and the relations among them are studied. The corner frequency and seismic moment are estimated from three different methods. The spectral fitting of the source displacement spectrum with the $\omega$-square source model of Brune(1970) and Snoke(1987)'s method are applied to all events and empirical Green's function method for two events are adopted. The source parameters determined in this study show different values depending on the adopted method and on the stations of which seismograms are recorded. It is interpreted that the disagreements principally originate from insufficient consideration of source radiation pattern and attenuation and amplification according to path direction. The corner frequencies and seismic moments are averaged to exclude the directional effects and other source parameters are estimated from the mean corner frequency and seismic moment. The static stress drops estimated in this study tend to be independent of seismic moment or magnitude for events above a certain size. For earthquakes with the size less than about 3.0$\times$10$^{21}$dyne-cm(nearly same as M$_{L}$=3.7), the stress drop tends to decrease with the decreasing moment. This fact suggests a breakdown of scaling law of source parameters below the threshold magnitude. The moment magnitudes calculated from source parameters appear to be slightly larger than the Richter's local magnitudes in the range above M$_{L}$=3.5.3.5.

• Journal of the Earthquake Engineering Society of Korea
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• v.22 no.5
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• pp.299-309
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• 2018

Now that problems with force-based seismic design have been clearly identified, design is inclined toward displacement-based methods. One such widely used method is Direct-Displacement-Based Design (DDBD). Yet, one of the shortcomings of DDBD is considering higher-mode amplification of story shear, moments, and displacements using equations obtained from limited parametric studies of regular planar frames. In this paper, a different approach to account for higher-mode effects is proposed. This approach determines the lateral secant stiffness of the building frames that fulfill the allowable inter-story drift without exceeding the desired story displacements. Using the stiffness, an elastic response spectrum analysis is carried out to determine elastic higher-mode force effects. These force effects are then combined with DDBD-obtained first-mode force effects using the appropriate modal superposition method so that design can be performed. The proposed design procedure is verified using Nonlinear Time History Analysis (NTHA) of twelve planar frames in four categories accounting for mass and stiffness irregularity along the height. In general, the NTHA response outputs compared well with the allowable limits of the performance objective. Thus, it fulfills the aim of minimizing the use of NTHA for planar frame buildings, thereby saving computational resources and effort.

• Journal of Genetic Medicine
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• v.10 no.2
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• pp.94-98
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• 2013

Dystrophinopathy, caused by mutations in the DMD gene, presents with variable clinical phenotypes ranging from the severe Duchenne muscular dystrophy (DMD) to the milder Becker muscular dystrophy(BMD) forms. DMD is a recessive X-linked form of muscular dystrophy. Two-thirds of mothers of affected males are thought to be DMD carriers. Approximately 2.5-7.8% of female DMD carriers have muscle weakness and are categorized as manifesting DMD carriers. The symptoms of female carriers of DMD range from mild muscle weakness to severe gait problems. The most commonly presented symptom is mild proximal muscle weakness, which is often asymmetric and progressive, but shows variable clinical spectrum with BMD of more severe DMD-like phenotype. Atypical presentations in manifesting carriers are myalgia or cramps without limb weakness, isolated cardiomyopathy and camptocormia. Multiplex PCR and MLPA analysis are common techniques to identify mutations in the DMD gene. Relationship between X-chromosome inactivation and clinical severity is not clear. Female carriers of DMD are not less common, and they have an important role of birth of a male DMD.

• Journal of the Earthquake Engineering Society of Korea
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• v.20 no.7_spc
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• pp.537-543
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• 2016

In low to moderate seismic regions, there are limited earthquake ground motion data recorded from past earthquakes. In this regard, the Gyeongju earthquake (M=5.8)occurred on September 12, 2016 produces valuable information on ground motions. Ground motions were recorded at various recording stations located widely in Korean peninsula. Without actual recoded ground motions, it is impossible to make a ground motion prediction model. In this study, a point source model is constructed to accurately simulate ground motions recorded at different stations located on different soil conditions during the Gyeongju earthquake. Using the model, ground motions are generated at all grid locations of Korean peninsula. Each grid size has $0.1^{\circ}(latitude){\times}0.1^{\circ}(longitude)$. Then a contour hazard map is constructed using the peak ground acceleration of the simulated ground motions.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2002.11a
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• pp.656-661
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• 2002

Multipass Ti:sapphire amplifier for the light source of lidar was developed in an angular-multiplexing, and the characteristics of output energy and spectrum was investigated. In the two-stage multipass amplifier, we obtained the maximum output energy of 42 mJ, the amplification gain of 21 dB and the output efficiency of 26% on the wavelength of 790 nm. In the tuning range of 715~930nm the spectral linewidth is 0.05 $cm^{-1}$ /. The conversion efficiencies of 35% for SHG at 780 m and 13% for THG at 390 nm are obtained respectively. The continuous tunabilities of 240~306 m UV region and 360~460 nm in deep-blue region could be achieved.

• Proceedings of the Earthquake Engineering Society of Korea Conference
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• 2006.03a
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• pp.327-336
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• 2006

Site coefficients in IBC and KBC codes have some limits to predict the rational seismic responses of a structure, because they consider only the effect of the soil amplification without the effects of the structure-soil interaction. In this study, upper and lower limits of site coefficients are estimated through the pseudo 3-D elastic seismic response analyses of structures built on linear or nonlinear soil layers considering the structure-soil interaction effects. Soil characteristics of site classes of A, B, and C were assumed to be linear, and those of site classes of D and E were done to be nonlinear and the Ramberg-Osgood model was used to evaluate shear modulus and damping ratio of a soil layer depending on the shear wave velocity of a soil layer. Seismic analyses were performed with 12 weak or moderate earthquake records, scaled the peak acceleration to 0.1g or 0.2g and deconvoluted as earthquake records at the bedrock 30m beneath the outcrop. With the study results of the elastic seismic response analyses of structures, new standard response spectrum and upper and lower limits of the site coefficients of Fa and Fv at the short period range and the period of 1 second are suggested Including the structure-soil interaction effects.

• Nuclear Engineering and Technology
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• v.53 no.4
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• pp.1345-1356
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• 2021

Damage to nuclear power plants causes human casualties and environmental disasters. There are electrical facilities that control safety-related devices in nuclear power plants, and seismic performance is required for them. The 2016 Gyeongju earthquake had many high-frequency components. Therefore, there is a high possibility that an earthquake involving many high frequency components will occur in South Korea. As such, it is necessary to examine the safety of nuclear power plants against an earthquake with many high-frequency components. In this study, the shaking table test of electrical facilities was conducted against the design earthquake for nuclear power plants with a large low-frequency components and an earthquake with a large high-frequency components. The response characteristics of the earthquake with a large high-frequency components were identified by deriving the amplification factors of the response through the shaking table test. In addition, safety of electrical facility against the two aforementioned types of earthquakes with different seismic characteristics was confirmed through limit-state seismic tests. The electrical facility that was performed to the shaking table test in this study was a motor control center (MCC).

• Journal of the Architectural Institute of Korea Structure & Construction
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• v.35 no.11
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• pp.129-136
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• 2019

Non-structural elements are vulnerable to earthquake ground motion. In this study, an experimental study for the electrical non-structural element was performed using tri-axial shaking table tests. A 100kVA UPS(Uninterruptible Power Supply system) was used as the test specimen. The test specimen was anchored to the concrete slab using the conventional installation detail. The input acceleration were generated in accordance with ICC-ES AC156 code. Scale factors of the input acceleration with respect to the required response spectrum defined in ICC-ES AC156 were from 25% to 600%. Based on the test results, damage and dynamic characteristics of UPS were evaluated and analyzed including natural frequency, damping ratio, acceleration time history response, dynamic amplification factor and relative displacement.