• Journal of Microbiology
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• v.44 no.4
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• pp.423-431
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• 2006

Among 53 Acinetobacter baumannii isolates collected in 2004, nine imipenem-resistant isolates were obtained from clinical specimens taken from patients hospitalized in Busan, Korea. Nine carbapenemase-producing isolates were further investigated in order to determine the mechanisms underlying resistance. These isolates were then analyzed via antibiotic susceptibility testing, microbiological tests of carbapenemase activity, pI determination, transconjugation test, enterobacterial repetitive consensus (ERIC)-PCR, and DNA sequencing. One outbreak involved seven cases of infection by A. baumannii producing OXA-23 ${\beta}-lactamase$, and was found to have been caused by a single ERIC-PCR clone. During the study period, the other outbreak involved two cases of infection by A. baumannii producing IMP-1 ${\beta}-lactamase$. The two clones, one from each of the outbreaks, were characterized via a modified cloverleaf synergy test and an EDTA-disk synergy test. The isoelectric focusing of the crude bacterial extracts detected nitrocefin-positive bands with pI values of 6.65 (OXA-23) and 9.0 (IMP-1). The PCR amplification and characterization of the amplicons via direct sequencing showed that the clonal isolates harbored $bla_{IMP-1}$ or $bla_{oxA-23}$ determinants. The two clones were characterized by a multidrug resistance phenotype that remained unaltered throughout the outbreak. This resistance encompassed penicillins, extended-spectrum cephalosporins, carbapenems, monobactams, and aminoglycosides. These results appear to show that the imipenem resistance observed among nine Korean A. baumannii isolates could be attributed to the spread of an IMP-lor OXA-23-producing clone. Our microbiological test of carbapenemase activity is a simple method for the screening of clinical isolates producing class D carbapenemase and/or class B $metallo-{\beta}-lactamase$, in order both to determine their clinical impact and to prevent further spread.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.33 no.2
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• pp.585-594
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• 2013

In this study, laboratory experiments and numerical simulations were conducted to test the performance of resonance power buoy system proposed by Kweon et al.(2010). The system is composed of a linear generator and a mooring buoy. The mover of the linear generator mainly has heave motion driven by vertical oscillation of the buoy. In this system, the velocity discrepancy between the mover and the buoy makes electricity. However, ocean wave energy as a natural resource around Korean peninsula is comparatively small and the driving force for producing electricity is not enough for commercialization. Therefore, it is necessary that the buoy motion be amplified by using resonance characteristics. In order to verify the resonance effects on the test power buoy, the experimental investigations were conducted in the large wave flume (length of 110 m, width of 8 m, maximum depth of 6 m) equipped with regular and random plunger wave generator. The resonance draft of test power buoy is designed for the corresponding period of incident wave, 1.96 sec. Regular wave test results show that the heave response amplitude operator(RAO) by a test buoy has the amplification of 5.66 times higher compared to the wave amplitude at the resonance period. Test results of random waves show that the buoy has the largest spectrum area of 20.73 times higher at the point of not the resonance period but the shorter one of 1.85 sec. Therefore this study suggests the resonance power buoy for wave power generation for commercial application in the case of the coastal and oceanic area with smaller wave energy.

• Journal of the Korea institute for structural maintenance and inspection
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• v.24 no.6
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• pp.17-24
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• 2020

In this study, series of nonlinear seismic analysis were performed on a reinforced concrete intake tower surrounded by water. To consider the fluid effect around the structure, analysis models were composed using an added mass and CEL approach. At this time, the implicit method was used for the added mass model, and the explicit method was used for the fluid structure interaction model. The input motions were scaled to correspond to 500, 1000, and 2400 years return period of the same artificial earthquake. To estimate the counteractivity of the fluid coupled model, models without fluid effect were constructed and used as a reference. The material models of concrete and reinforcement were selected to consider the nonlinear behavior after yielding, and analysis were performed by ABAQUS. As results, in the acceleration response spectrum of the structure, it was found that the influence of the surrounding fluid reducing the peak frequency and magnitude corresponding to the fundamental frequency of the structure. However, the added mass model did not affect the peak value corresponding to the higher mode. The sectional moments were increased significantly in the case of the added mass model than those of the reference model. Especially, this amplification occurred largely for a small-sized earthquake response in which linear behavior is dominant. In the fluid structure interaction model, the sectional moment with a low frequency component amplifies compared to that of the reference model, but the sectional moment with a high requency component was not amplified. Based in these results, it was evaluated that the counteractivity of the additive mass model was greater than that of the fluid structure interaction model.