• Journal of Biosystems Engineering
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• v.38 no.4
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• pp.312-317
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• 2013

Purpose: Conventional methods used to evaluate seeds viability are destructive, time consuming, and require the use of chemicals, which are not feasible to implement to process plant in seed industry. In this study, the effectiveness of Fourier transform near infrared (FT-NIR) spectroscopy to differentiate between viable and nonviable watermelon seeds was investigated. Methods: FT-NIR reflectance spectra of both viable and non-viable (aging) seeds were collected in the range of 4,000 - 10,000 $cm^{-1}$ (1,000 - 2,500 nm). To differentiate between viable and non-viable seeds, a multivariate classification model was developed with partial least square discrimination analysis (PLS-DA). Results: The calibration and validation set derived from the PLS-DA model classified viable and non-viable seeds with 100% accuracy. The beta coefficient of PLS-DA, which represented spectral difference between viable and non-viable seeds, showed that change in the chemical component of the seed membrane (such as lipids and proteins) might be responsible for the germination ability of the seeds. Conclusions: The results demonstrate the possibility of using FT-NIR spectroscopy to separate seeds based on viability, which could be used in the development of an online sorting technique.

• Applied Biological Chemistry
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• v.41 no.2
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• pp.153-159
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• 1998

A possibility of evaluation of the major internal quality factors-Brix, moisture contents, firmness and acid content in the Korean domestic 'Fuji'apple fruits by near-infrared reflectance spectroscopic (NIRS) methods were investigated. A multiple linear regression(MLR) analysis between the data obtained by physico- chemical analysis method using refractometer, freeze drier, texture analyzer and titrater and NIR spectral data was carried out to make a calibration. The standard error of prediction(SEP) of Brix, moisture, firmness and acid content were $0.50^{\circ}Brix,\;0.64%,\;0.14kg/cm^2$ and 0.07%. It is concluded that NIRS methods can be used to evaluate Brix and moisture contents of in a apple non-destructive and rapid way but the accuracy for determination of firmness and acid content was slightly low.

• Journal of Sasang Constitutional Medicine
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• v.19 no.2
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• pp.113-126
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• 2007

1. Objectives The objective of this study was to measure the differences of EEG changes accoring to emotional stimulus in Sasangin. 2. Methods 44 healthy volunteers(Soyangin 10, Soeumin 14, Taeumin 20) were participated. Sasang Constitution was diagnosed by Sasang specialist. Volunteers watched horror movie as the emotional stimulus. We recorded EEG data during pre-stimulus rest(120sec), during-stimulus(197sec), rest-stimulus(120sec). Power spectral analysis was done and relitive power and ${\alpha}/{\beta}$ ratio were compared among each groups. 3. Results (1) The mean of relative ${\alpha}$ of Taeumin was significantly decreased according to stimulus compared with Soyagnin. (2) Relative ${\alpha}$ of Soeumin was significantly decreased in T3 area according to stimulus compared with Taeumin. (3) Relative ${\beta}$ of Taeumin group was significantly increased in Fp2 area according to stimulus compared with Soeumin. 4) Relative $high-{\beta}$ of Soyangin group was significantly decreased in F3 by the post-stimulus rest compared with Soeumin. (5) ${\alpha}/{\beta}$ ratio of Soeumin was significantly increased according to stimulus compared with other groups. 4. Conclusions Soyangin showed sensitive changes according to the emotional simulus compared with Soeumin and Taeumin. Above results suggest that the emotional characteristics of Sasangin can be measured by objective methods as by EEG.

• Journal of Ginseng Research
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• v.38 no.3
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• pp.194-202
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• 2014

Background: Ginsenosides, the major ingredients of Panax ginseng, have been studied for many decades in Asian countries as a result of their wide range of pharmacological properties. The less polar ginsenosides, with one or two sugar residues, are not present in nature and are produced during manufacturing processes by methods such as heating, steaming, acid hydrolysis, and enzyme reactions. $^1H$-NMR and $^{13}C$-NMR spectroscopic data for the identification of the less polar ginsenosides are often unavailable or incomplete. Methods: We isolated 21 compounds, including 10 pairs of 20(S) and 20(R) less polar ginsenosides (1-20), and an oleanane-type triterpene (21) from a processed ginseng preparation and obtained complete $^1H$-NMR and $^{13}C$-NMR spectroscopic data for the following compounds, referred to as compounds 1-21 for rapid identification: 20(S)-ginsenosides Rh2 (1), 20(R)-Rh2 (2), 20(S)-Rg3 (3), 20(R)-Rg3 (4), 6'-O-acetyl-20(S)-Rh2 [20(S)-AcetylRh2] (5), 20(R)-AcetylRh2 (6), 25-hydroxy-20(S)-Rh2 (7), 25-hydroxy-20(S)-Rh2 (8), 20(S)-Rh1 (9), 20(R)-Rh1 (10), 20(S)-Rg2 (11), 20(R)-Rg2 (12), 25-hydroxy-20(S)-Rh1 (13), 25-hydroxy-20(R)-Rh1 (14), 20(S)-AcetylRg2 (15), 20(R)-AcetylRg2 (16), Rh4 (17), Rg5 (18), Rk1 (19), 25-hydroxy-Rh4 (20), and oleanolic acid 28-O-b-D-glucopyranoside (21).

• International Journal of Oral Biology
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• v.36 no.2
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• pp.59-64
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• 2011

Anthocyanins are naturally occuring phytochemicals and the main components of the coloring of plants, flowers and fruits. They are known to elicit antioxidative, anti-inflammatory and cancer preventive activity. In this study, we investigated anthocyanins in black / yellow soybean seedcoats using different methods of detection - thin layer chromatography (TLC), capillary zone electrophoresis (CZE) and HPLC analysis. The anthocyanins in soybean seedcoats were extracted by five independent methods of extraction and the aglycons (anthocyanidins) of the corresponding anthocyanins were prepared by acid mediated hydrolysis. The anthocyanin / anthocyanidin in black soybean seedcoat showed characteristic TLC mobility, CZE electrophoretic retention and HPLC migration time while little of anthocyanins were detected from yellow soybean seedcoat. The extracted anthocyanins showed pH dependent retention time in CZE and spectral change in UV-Vis spectrum. HPLC analysis of the hydrolyzed extract of black soybean seedcoat identified the presence of four anthocyanidins. The major anthocyanin in black soybean seedcoat was cyanin (cyanidin-3-O-glucoside), with the relative order of anthocyanidin in cyanidin > delphinidin > petunidin > pelargonidin.

• Korean Journal of Optics and Photonics
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• v.20 no.6
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• pp.334-338
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• 2009

Recently, with increasing demand for flat-panel display product, methods for large area patterning are required. TIR (total internal reflection) holographic photo-lithography isstudied as one of the methods of large area lithography. In conventional TIR holography, light sources for hologram recording and image reconstruction are coherent beams such as laser beams. If the image is reconstructed with an incoherent light source such a UV lamp, the image noise from the coherence of light will be reduced and the UV lamp will be a better light source for large area exposure. We analyzed the effect of spectral bandwidth and angular bandwidth of the light source in image reconstruction and verified image blurring with experiments. For large area patterning which has micro-scale line width, it is expected that TIR holographic photo lithography by UV lamp will become a low-noise and low-priced technique.

• Proceedings of the Korea Institute of Convergence Signal Processing
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• 2003.06a
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• pp.6-9
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• 2003

In this paper, we studied on the difference of speaker adaptation according to the phoneme classification for Korean Speech recognition. In order to study of speech adaptation according to the weight of difference of phoneme as recognition unit, we used SCHMM as recognition system. And Speaker adaptation method used in this paper was MAPE(Maximum A Posteriori Probability Estimation), Linear Spectral Estimation. In order to evaluate the performance of these methods, we used 10 Korean isolated numbers as the experimental data. It is possible for the first and the second methods to be carried out unsupervised learning and used in on-line system. And the first method was shown performance improvement over the second method, and hybrid adaptation showed the better recognition results than those which performed each method. And the result of Speaker adaptation using the variable weight according to the phoneme had better than the result using fixed weight.

• Proceedings of the KSRS Conference
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• 2002.10a
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• pp.708-708
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• 2002

Recent developments in sensor technologies have provided remotely sensed data with very high spatial resolution. In order to fully utilize the potential of high resolution images, new image classification strategies are necessary. Unfortunately, the high resolution images increase the spectral within-field variability, and the classification accuracy of traditional methods based on pixel-based classification algorithms such as Maximum-Likelihood method may be decreased (Schiewe 2001). Recent development in Object Oriented Classification based on image segmentation algorithms can be used for the classification of forest patches on rugged terrain of Korea. The objectives of this paper are as follows. First, to compare the pros and cons of image classification methods based on pixel-based and object oriented classification algorithm for the forest patch classification. Landsat ETM＋ data and IKONOS data will be used for the classification. Second, to investigate ways to increase classification accuracy of forest patches. Supplemental data such as DTM and Forest Type Map of 1:25,000 scale are used for topographic correction and image segmentation. Third, to propose the best classification strategy for forest patch classification in terms of accuracy and data requirement. The research site for this paper is Namhansansung Provincial Park located at the eastern suburb of Seoul Metropolitan City for its diverse forest patch types and data availability. Both Landsat ETM＋ and IKONOS data are used for the classification. Preliminary results can be summarized as follows. First, topographic correction of reflectance is essential for the classification of forest patches on rugged terrain. Second, object oriented classification of IKONOS data enables higher classification accuracy compared to Landsat ETM＋ and pixel-based classification. Third, multi-stage segmentation is very useful to investigate landscape ecological aspect of forest communities of Korea.

• Proceedings of the KSRS Conference
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• 2003.11a
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• pp.654-655
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• 2003

Korea has experienced 10 a Cochlodinium polykrikoides red tide outbreaks during the last 10 years (1993-2002). The monitoring activities at National Fisheries Research and Development Institute (NFRDI) in Korea have been extended to all the coastal waters after the worst of fish killing by C. polykrikoides blooms in 1995. NFRDI is looking forward to finding out the feasibility of red tide detection around Korean waters using satellite remote sensing of NOAA/AVHRR, Orbview-2/SeaWiFS, IRS-P4/OCM and Terra/MODIS on real time base. In this study, we used several alternative methods including climatological analysis, spectral and optical methods which may offer a potential detection of the major species of red tide in Korean waters. The relationship between the distribution of SST and C. polykrikoides bloom areas was studied. In climatological analysis, NOAA, SeaWiFS, OCM satellite data in 20th and 26th August 2001 were chosen using the known C. polykrikoides red tide bloom area mapped by helicopter reconnaissance and ground observation. The 26th August, 2001 SeaWiFS chlorophyll a anomaly imageries against the imageries of non-occurring red tide for August 20, 2001 showed the areas C. polykrikoides occurred. The anomalies of chlorophyll a concentration from satellite data between before and after red tide outbreaks showed the similar distribution of C. polykrikoides red tide in 26th August, 2001. The distribution of the difference in SST between daytime and nighttime also showed the possibility of red tide detection. We used corrected vegetation index (CVI) to detect floating vegetation and submerged vegetation containing algal blooms. The simple result of optical absorption from C. polykrikoides showed that if we use the optical characteristics of each red tide we will be able to get the feasibility of the red tide detection.

• Journal of Preventive Medicine and Public Health
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• v.39 no.2
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• pp.165-170
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• 2006

Objectives : The determination of oxalate in urine is required for the diagnosis and treatment of primary hyperoxaluria, idiopathic stone disease and various intestinal diseases. We examined the possibility of using Fourier transform near-infrared (FT-NIR) spectroscopy analysis to quantitate urinary oxalate. The practical advantages of this method include ease of the sample preparation and operation technique, the absence of sample pre-treatments, rapid determination and noninvasiveness. Methods : The range of oxalate concentration in standard urine solutions was $0-221mg/{\ell}$. These 80 different samples were scanned in the region of 780-1,300 nm with a 0.5 nm data interval by a Spectrum One NTS FT-NIR spectrometer. PCR, PLSR and MLR regression models were used to calculate and evaluate the calibration equation. Results : The PCR and PLSR calibration models were obtained from the spectral data and they are exactly same. The standard error of estimation (SEE) and the % variance were $10.34mg/{\ell}$ and 97.86%, respectively. After full cross validation of this model, the standard error of estimation was $5,287mg/{\ell}$, which was much smaller than that of the pre-validation. Furthermore, the MCC (multiple correlation coefficient) was 0.998, which was compatible with the 0.923 or 0.999 obtained from the previous enzymatic methods. Conclusions : These results showed that FT-NIR spectroscopy can be used for rapid determination of the concentration of oxalate in human urine samples.