• 약학회지
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• 제43권6호
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• pp.709-715
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• 1999

Polyphenol oxidase (PPO) activity in 200×g (cell wall), 4,000×g (plastid), 100,000×g (mitochondrial) and soluble fractions of the perilla leaves was monitored in the upper, middle and lower sections of the plant. In the course of plant growth, PPO activities in plastid and mitochondrial fractions were decreased, while those in cell wall fraction were maintained. During growing process, specific activities and PPO activities of each fraction were decreased, while total phenol content were decreased in middle (middle) and then increased in later stage (lower). Cell wall, plastid, mitochondrial (pellet) and soluble fraction had slightly different pH optima and substrate specificities. Isoenzyme patterns were identical in two bands for PPO activity in different subcellular fractions. Their molecular weights were 37KD and 48KD respectively.

• 미생물학회지
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• 제26권1호
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• pp.52-59
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• 1988

A partially purified preparation of L-galactonolactone oxidase which catalyzes the last step of L-ascorbic acid biosynthesis was obtained from Saccharomyces cerevisiae ATCc 26787. The purification procedures included Triton X-100 treatment, protamine sulfate precipitation, ammonium sulfate precipitation, DEAE-Sepharose CL-6B ion exchange chromatography, Sephadex G-150 gel filtration chromatography, and Phenyl-Sepharose CL-4B hydrophobic interaction chromatography. The optimum temperature for the enzyme activity was about $34^{\circ}C$ and the optimum pH was 6.8-7.0. The substrate specificity was confined to L-aldonolactones, L-galactono-1,4-lactone and L-gulono-1,4-lactone. An apparent Km value of 0.294mM with L-galactono-1,4-lactone as a substrate was found. By comparing the substrate specificities of this enzyme with those of isofunctional enzymes of higher plants and animals, it becomes evident that the enzyme of S. cerevisiae ATCC 26787 is rather similar to the L-gulonolactone oxidase of animals than the galactonolactone dehydrogenase of higher plants.

• Journal of information and communication convergence engineering
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• 제2권1호
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• pp.15-21
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• 2004

One of the most important issues in designing a network and realizing a service is dealing with traffic characteristics. Recent experimental research on LAN, WAN, and VBR traffic properties has highlighted that real traffic specificities can not be displayed because the current models based on the Poisson assumption under estimate the long range dependency of network traffic and self-similar peculiarities. Therefore, a new approach using self-similarity characteristics as a real traffic model was recently developed. In This paper we discusses the definition of self-similarity traffic. Moreover, real traffic was collected and we generated self-similar data traffic like real traffic to background load. On the existing ABR congestion control algorithm transmission throughput with the representative ERICA, EPRCA and NIST switch algorithm show the efficient reaction about the burst traffic.

• Computers and Concrete
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• 제13권5호
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• pp.649-657
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• 2014

In this paper, effects of magnetic water on different properties of cement paste including fluidity, compressive strength, time of setting and etc, has been studied in concrete laboratory of Sahand University of Technology. For production of magnetic water, three devices including an AFM called device(made in UAE), a device marked AC(made in Germany) and finally a device was designed and made in Concrete Laboratory of Sahand University of Technology) have been used. The results show that, intensity and direction of magnetic field, velocity and time of water passing through magnetic device, and amount and type of Colloidal particles have direct effects on properties of magnetic water and using such a water in making cement paste, increases its fluidity and compressive strength up to 10%.

• 비교문화연구
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• 제23권
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• pp.127-165
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• 2011

This paper focuses on the specificities and semantic meaning of Mikhail Bulgakov's metadrama White Guard and The Flight. The standard conception of metadrama is to purposefully break the dramatic illusion and make bare a playwright's self-consciousness of the theatrical art itself. With the use of the metadrama Bulgakov expressed the essentials of ugly reality, which he couldn't accept as a valuable truth. In this respect, Bulgakov's metadrama becomes at once a window, from which he views the external world in the theatrical vision, and a mirror, in which his political and existential stance as a playwright is reflected. In White Guard Bulgakov described the already theatricalized reality through several instances of 'play-within-play'. In The Flight, composed of eight pieces of dream, a life turned out to be a less solid and less firm reality than dream. Continuously demolishing the cognitive wall between reality and illusion, Bulgakov leads spectators to have a reflective view on the reality. Allowing more powerful demonstrativeness for a play-within-play than for a play-within-play, Bulgakov elevates a metadramatic technique to the level of thematic structure.

• 미국학
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• 제44권2호
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• pp.67-96
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• 2021

The Canadian public broadcaster CBC had successfully aired a sitcom centered on a Korean immigrant family from 2016 to 2021. The show is based on the play Kim's Convenience written by a Korean-Canadian playwright Ins Choi. This study explores literary features of Kim's Convenience that accounts for its popularity; three elements of the show play crucial roles in maintaining the balance between specificity and universality. First, Choi deploys a Korean immigrant story in the form of comedy. Second, the main plot revolves around an ordinary family with generational strains that ends in reconciliation. Third. the Kims are depicted more as an archetypical family than a stereotypically Asian one. By closely interweaving these elements, Choi induces the audience to find commonalities from the show, and racial specificities of the Kim's family become "spicy" attractions of the play.

• 국제학술발표논문집
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• The 6th International Conference on Construction Engineering and Project Management
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• pp.656-657
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• 2015

Recently, Multi-Objective Optimization of design elements is an important issue in building design. Design variables that considering the specificities of the different environments should use the appropriate algorithm on optimization process. The purpose of this study is to compare and analyze the optimal solution using three evolutionary algorithms and energy modeling simulation. This paper consists of three steps: i)Developing three evolutionary algorithm model for optimization of design elements ; ii) Conducting Multi-Objective Optimization based on the developed model ; iii) Conducting comparative analysis of the optimal solution from each of the algorithms. Including Non-dominated Sorted Genetic Algorithm (NSGA-II), Multi-Objective Particle Swarm Optimization (MOPSO) and Random Search were used for optimization. Each algorithm showed similar range of result data. However, the execution speed of the optimization using the algorithm was shown a difference. NSGA-II showed the fastest execution speed. Moreover, the most optimal solution distribution is derived from NSGA-II.

• Journal of Gastric Cancer
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• 제9권3호
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• pp.110-116
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• 2009

목적: 위암환자의 수술 전 병기 결정에 있어서 PET의 유용성을 알아보기 위해 본 연구를 시행하였다. 대상 및 방법: 2006년 2월부터 2008년 8월까지 본원에서 위암으로 진단받고 수술 전 병기 결정을 위해 복부 컴퓨터 단층촬영(CT) 및 PET-CT를 모두 시험한 환자 70명을 대상으로 복부 CT 및 PET-CT 영상 자료에 의한 림프절 전이 판정과 수술 후 병리 조직검사에 의한 림프절 전이판정으로 민감도, 특이도, 양성예측도, 음성예측도 및 정확도를 조사하였고 위암의 형태, 조직학적 분류, 크기 등과 FDG섭취 정도와의 차이 등을 알아보았다. 결과: 조기 위암 23예의 경우 복부 CT에서 병변이 진단된 경우는 4명(17.4%), PET-CT에서는 12명(52.1%)이었으며, 진행 위암 47예의 경우 CT에서 26명(56.5%), PET-CT에서 45명(95.7%)으로 PET-CT에서 진단율이 높게 나타났다. 병변의 크기에 따른 분류에서도 복부 CT보다 PET-CT에서 진단율이 높은 것으로 나타났으며, 조기 위암의 경우 진행 위암에 비해 FDG 섭취 정도가 낮았지만 3 cm 이상의 조기 위암의 경우 3 cm 미만의 조기 위암에 비해 FDG가 섭취되는 경우가 더 많았다. CT에서 림프절 병기 결정의 민감도는 40.0%, 특이도는 85.7%, 양성예측도는 85.7%, 그리고 음성예측도는 40.0%였으며, PET-CT에서는 각각 55.6%, 81.0%, 86.2%, 45.9%였다. PET-CT를 통해서만 발견된 전이 병소는 1예(간전이)였으며, 중복암은 직장암 1예와 췌장암 1예가 있었다. 또한 세포조직형에 따른 분류에서도 분화영, 미분화형 모두 PET-CT에서 진단율이 높은 것으로 나타났다. 결론: CT에 비해 PET-CT에서 원발 병소의 진단, 림프절 전이 모두 진단율이 높은 것으로 나타났으며 PET-CT는 전이 병소와 중복암의 발견 등 다른 영상 진단법에 비하여 유리한 점이 있다.

• 대한영상의학회지
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• 제79권6호
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• pp.340-347
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• 2018

목적: 가도세틱산-조영증강 MRI에서 간세포암 피막 발견에 대한 영상차감기법의 진단적 가치를 알아보고자 하였다. 대상과 방법: 2015년 5월부터 2017년 2월까지 가도세틱산-조영증강 MRI를 시행 받고 수술을 시행한 hepatocellular carcinoma (이하 HCC) 고위험군 108명을 대상으로 하였다. 차감영상의 질 및 간문맥기와 이행기의 일반영상과 차감영상에서 피막 여부에 대해 평가하였고, 차감영상에서의 피막 여부가 Liver Imaging Reporting and Data System에 따른 간세포암 진단에 미치는 영향을 평가하였다. 결과: 수술 전 경동맥화학색전술을 시행 받았거나 차감영상의 질이 불만족스러운 34명의 환자를 제외한 74명의 환자에서 82개의 간 병변(간세포암 73개, 그 외 악성종양 5개, 양성종양 4개)에 대해 분석하였다. 피막의 발견에 대한 차감영상의 민감도, 정확도, 그리고 곡선하면적은 일반영상과 비교하여 통계적으로 유의하게 높았고(각 95.4%, 89.0%, 0.80; p < 0.001), 특이도는 동일하였다(64.7%). HCC의 진단에 대해서도 차감영상이 일반영상과 비교하여 민감도, 정확도, 그리고 곡선하면적이 통계적으로 유의하게 높았으며(각 82.2%, 79.3%, 0.69; p = 0.011), 특이도는 동일하였다(55.6%). 결론: 가도세틱산-조영증강 MRI에서 간문맥기 또는 이행기로부터의 차감영상은 간세포암 피막의 발견에 도움이 된다.

• 대한본초학회지
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• 제34권5호
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• pp.13-20
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• 2019

Objectives : To ensure the safety, quality and pharmacological efficacy of Batryticatus Bombyx, it is important to discriminate with adulterants. In Korean Herbal Pharmacopoeias (KHP), the authentic species of Batryticatus Bombyx is defined only Bombyx mori. Therefore, the aim of this study is establishment of PCR assay method using the sequence characterized amplified region (SCAR) marker based on COI DNA barcode for discriminating six species related to Batryticatus Bombyx. Methods : Seventeen samples of six species (Bombyx mori, Bombyx mandarina, Rhodinia fugax, Oberthueria caeca, Actias artemis, and Caligula japponica) were collected from different habitate and nucleotide sequences of cytochrome c oxidase subunit I(COI) barcode regions were analyzed by Sanger sequencing methods. To develop SCAR-based PCR assay method, we designed species-specific primers based on COI sequence variabilities and verified those specificities using 17 samples of six species as well as commercial herbal medicines. Results : In comparative multiple analysis of COI sequences, six species were distinguished by species-specific nucleotides at the species level. To develop rapid and reliable PCR assay method for genetic authentication of Batryticatus Bombyx, therefore, we designed species-specific SCAR primers based on these nucleotide sequences and confirmed those specificities. Using these SCAR primers, We also established simple conventional PCR assay method using these SCAR primers at the species level. Conclusions : The comparative analysis of COI sequences and SCAR-based PCR assay methods represented equal results for distinguishing authentic Batryticatus Bombyx and adulterations at the species level. Therefore, our results are expected protecting adulteration of herbal medicine Batryticatus Bombyx.