• Journal of Korean Tunnelling and Underground Space Association
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• v.25 no.3
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• pp.201-220
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• 2023

Power cable tunnels is one of the underground structures meant for electricity transmission and are constructed using shield TBM method when transitting across urban and subsea regions. With the increasing shaft depth for tunnels excavation when the shield TBM excavated the rock mass, the review of selecting closed-type shield TBM in rocks becomes necessary. A simplified shield TBM design method is also necessary based on conventional geotechnical survey results. In this respect, design method and related design program are developed based on combined results of full-scale tests, considerable amount of accumulated TBM data, and numerical simulation results. In order to validate the program results, excavation data of a completed power cable tunnel project are utilized. Thrust force, torque, and power of shield TBM specification are validated using Kernel density concept which estimates the population data. The robustness of design expertise is established through this research which will help in stable provision of electricity supply.

• Journal of Service Research and Studies
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• v.13 no.3
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• pp.105-126
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• 2023

The domestic used car market continues to grow along with the used car online platform service. The used car online platform service discloses vehicle specifications, accident history, inspection history, and detailed options to service consumers. Most of the preceding studies were predictions of used car prices using vehicle specifications and some options for vehicles. As a result of the study, it was confirmed that there was a nonlinear relationship between used car prices and some specification variables. Accordingly, the researchers tried to solve the nonlinear problem by executing a Machine Learning model. In common, the Regression based Machine Learning model had the advantage of knowing the actual influence and direction of variables, but there was a disadvantage of low Cost Function figures compared to the Decision Tree based Machine Learning model. This study attempted to predict used car prices of six domestic brands by utilizing both vehicle specifications and vehicle options. Through this, we tried to collect the advantages of the two types of Machine Learning models. To this end, we sequentially conducted a regression based Machine Learning model and a decision tree based Machine Learning model. As a result of the analysis, the practical influence and direction of each brand variable, and the best tree based Machine Learning model were selected. The implications of this study are as follows. It will help buyers and sellers who use used car online platform services to predict approximate used car prices. And it is hoped that it will help solve the problem caused by information inequality among users of the used car online platform service.

• Economic Analysis
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• v.27 no.1
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• pp.67-90
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• 2021

This study found an interesting fact that the nonlinear relationship structure between volatility and trading volume changed before and after the COVID-19 pandemic according to empirical analysis using Bitcoin (BTC) market data that sensitively reflects investors' trading behavior. That is, their relationship appeared positive (+) in a stable market state before COVID-19 pandemic, as in theory based on the information flow paradigm. In a state under severe market stress due to COVID-19 pandemic, however, their dependence structure changed and even negative (-). This can be seen as a consequence of increased market stress caused by COVID-19 pandemics from a behavioral economics perspective, resulting in structural changes in the asset market and a significant impact on the nonlinear dependence of volatility and trading volume (in particular, their dependence at extreme quantiles). Hence, it should be recognized that in addition to information flows, psychological phenomena such as behavioral biases or herd behavior, which are closely related to market stress, can be a key in changing their dependence structure. For empirical analysis, this study performs a test of Ross (2015) for detecting a structural change, and proposes a Copula Regression Quantiles (CRQ) approach that can identify their nonlinear relationship structure and the asymmetric dependence in their distribution tails without the assumption of i.i.d. random variable. In addition, it was confirmed that when the relationship between their extreme values was analyzed by linear models, incorrect results could be derived due to model specification errors.

• Proceedings of the Korean Society of Developmental Biology Conference
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• 2003.10a
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• pp.84-84
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• 2003

DNA methylation is involved in epigenetic processes such as X-chromosome inactivation, imprinting and silencing of transposons. DNA methylation is a highly plastic and critical component of mammalian development The DNA methyltransferases (Dnmts) are responsible for the generation of genomic methylation patterns, which lead to transcriptional silencing. The maintenance DNA methyltransferase enzyme, Dnmt 1, and the de novo methyltransferase, Dnmt3a and Dnmt3b, are indispensable for development because mice homozygous for the targeted disruption of any of these genes are not viable. The occurrence of DNA methylation is not random, and it can result in gene silencing The mechanisms underlying these processes are poorly understood. It is well established that DNA methylation and histone deacetylation operate along a common mechanistic pathway to repress transcription through the action of methyl-binding domain proteins (MBDs), which are components of, or recruit, histone deacetylase (HDAC) complexes to methylated DNA. As a basis for future studies on the role of the DNA-methyl-transferase in porcine development, we have isolated and characterized a partial cDNA coding for the porcine Dnmt1. Total RNA of testis, lung and ovary was isolated with TRlzol according to the manufacture's specifications. 5 ug of total RNA was reverse transcribed with Super Script II in the presence of porcine Dnmt 1 specific primers. Standard PCRs were performed in a total volume of 50 ul with cDNA as template. Two DNA fragmenets in different position were produced about 700bp, 1500bp and were cloned into pCR II-TOPO according to the manufacture's specification. Assembly of all sequences resulted in a cDNA from 158bp of 5'to 4861bp of 3'compare with the known human maintenance methyltransferase. Now, we are cloning the unknown Dnmt 1 region by 5'-RACE method and expression of Dnmt 1 in tissues from adult porcine animals.

• Journal of the Korean Geosynthetics Society
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• v.22 no.1
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• pp.87-96
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• 2023

There are a lot of difference between the surface settlement and the differential settlement measured at the Busan New Port, where the dredged and reclaimed clay layer exists and below the clay is originally thickly distributed. To find the cause and solution of this, the actual conditions of each differential settlement used for the soft ground improvement, characteristics, installation method, measurement frequency, measurement data management, and data analysis of each type were considered. In the deep soft ground improvement work where large deformation occurs, the bending deformation of the screw-type differential settlement gauge is less than that of other types of measuring instruments, so there is less risk of loss, and the reliability of data is relatively high as the instruments are installed by drilling for each stratum. Since the greater the amount of high-precision settlement measurement data, the higher the settlement analysis precision. It is necessary to manage with higher criteria than the measurement frequency suggested in the standard specification. For the data management of the differential settlement gauge, it is desirable to create graphs of the settlement and embankment height of the relevant section over time, such as surface, differential, and settlement of pore water pressure gauge for each point. In the case of multi-layered ground with different compression characteristics, it is more appropriate to perform settlement analysis by calculating the consolidation characteristics of each stratum using a differential settlement data.

• Animal Bioscience
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• v.36 no.8
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• pp.1180-1189
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• 2023

Objective: Discovering the mechanism of cell specification is important to manipulate cellular lineages. To obtain lineage-specific cell lines, the target lineage needs to be promoted, and counterpart lineages should be suppressed. Embryos in the early blastocyst stage possess two different cell populations, the inner cell mass (ICM) and trophectoderm. Then, cells in the ICM segregate into epiblasts (Epi) and primitive endoderm (PrE). PrE cells in embryos show specific expression of platelet-derived growth factor (PDGF) and its receptor, PDGF receptor A (PDGFRA). In this study, we suppressed PDGF signaling using two methods (CRISPR/Cas9 injection and inhibitor treatment) to provide insight into the segregation of embryonic lineages. Methods: CRISPR/Cas9 RNAs were injected into parthenogenetically activated and in vitro fertilized embryos. The PDGF receptor inhibitor AG1296 was treated at 0, 5, 10, and 20 µM concentration. The developmental competence of the embryos and the number of cells expressing marker proteins (SOX2 for ICM and SOX17 for PrE) were measured after the treatments. The expression levels of the marker genes with the inhibitor were examined during embryo development. Results: Microinjection targeting the PDGF receptor (PDGFR) A reduced the number of SOX17-positive cell populations in a subset of day 7 blastocysts (n = 9/12). However, microinjection accompanied diminution of Epi cells in the blastocyst. The PDGF receptor inhibitor AG1296 (5 µM) suppressed SOX17-positive cells without reducing SOX2-positive cells in both parthenogenetic activated and in vitro fertilized embryos. Within the transcriptional target of PDGF signaling, the inhibitor significantly upregulated the Txnip gene in embryos. Conclusion: We identified that PDGF signaling is important to sustain the PrE population in porcine blastocysts. Additionally, treatment with inhibitors was a better method to suppress PrE cells than CRISPR/Cas9 microinjection of anti-PDGF receptor α gene, because microinjection suppressed number of Epi cells. The PDGF receptor might control the number of PrE cells by repressing the proapoptotic gene Txnip. Our results can help to isolate Epi-specific cell lines from blastocysts.

• Journal of Korea Trade
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• v.26 no.6
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• pp.1-19
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• 2022

Purpose - This study empirically investigates the effects of Official Development Assistance (ODA) on the economic activities of private actors in recipient countries. As a proxy for the economic activities of private actors, we utilize the job creation activities of foreign subsidiaries in recipient countries. The foreign subsidiaries provide a foundation for economic development by creating paying jobs. That is, if ODA has been successfully transferred to foreign subsidiaries, then these foreign subsidiaries should help economic growth and help create a boom in the local market by providing jobs. These jobs eventually lead to the achievement of the primary aims of foreign aid, including poverty reduction. Thus, this study empirically examines the relationship between ODA and the number of jobs created by foreign subsidiaries in recipient countries. Design/methodology - This is the first study to examine the effects of the ODA on the job creation of foreign subsidiaries because it has been hard to obtain internal information related to the employment status of foreign subsidiaries. Fortunately, we have a unique panel dataset provided by the Export-Import Bank of Korea (KEXIM) for 2006 to 2013. In terms of the empirical specification, we use the generalized least squares (GLS) method. The panel GLS estimator allows us to have an efficient estimation that overcomes the limitations of the panel data. It employs assumptions about the heteroscedasticity between the panels and makes an autocorrelation of the error term within each panel. Findings - We find that ODA influences job creation in foreign subsidiaries. In particular, we found that ODA creates more jobs in sales than in managerial or production positions. This study also shows that the effect of the ODA on the foreign subsidiaries' job creation activities depend on the purpose of the ODA. By examining ODA effects on the foreign subsidiaries' economic activities (e.g., job creation), this study fills a gap in the current literature. Originality/value - Existing studies that focus on the ODA effect have either a macroeconomic point or a microeconomic point of view. However, both approaches do not explain how well foreign aid has influenced private economic actors of recipient countries. In essence, previous researchers found it difficult to obtain the necessary data for internal employment status from foreign subsidiaries. However, thanks to the Korea Export-Import Bank, this study shows that ODA indeed influences the job creation activities of foreign subsidiaries even after controlling for other factors such as FDI, GDP growth rate, employment rate, household expenditure, mother firms' share, etc. By doing so, we can examine how ODA influences the job creation of foreign subsidiaries, which might help economic development and reduce the amount of poverty in recipient countries.

• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.41 no.1
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• pp.21-34
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• 2023

This study identified the materials and construction methods of 'Old Wall' in 13 villages which were designated as National Registered Cultural Heritage at the time of designation and examined the their structural changes based on field survey. The results are as follows: First, the 'Old Wall' consisted of 10 Soil-Stone Wall and 5 Stone Wall. At the time of designation, Stone Wall, which was built irregularly by dry-construction of natural stones, is similar in shape, but Soil-Stone Wall showed difference by the construction method of making used stones, joints, and faces. Second, the study extracted the changes of 'Old Wall' by repair and examined the changes of construction methods as well as the substitution and addition of materials of structure. The wall-roof was built with cement roof-tile and asbestos slate which have the advantage improve durability and cost-effectiveness. In addition, tile-mouth soil was added to korean traditional roof-tile to prevent rainwater from flowing in. Besides, to improve constructional convenience, the natural stone of the wall-body was replaced with blast stone, float stone and cut stone. Cement block, cement brick and cement mortar were frequently used to repair as well. As Soil-Stone Wall was transformed from irregular pattern-construction to comb pattern-construction and wet-construction was changed to dry-construction, it caused landscape and structural problems. Also, the layer of cement mortar applied to wall-foundation blocked the flow of rainwater that was induced by dry-construction of natural stones. Third, the study regarded that the problem with the repair of 'Old Wall' may occur as it is located in living space, because the owner of the wall could repair for the minor damages without technical knowledge. In addition, it is difficult for repair companies in charge of maintenance of Cultural Heritage to supply local materials, and it is differential construction specifications are not applied.

• Journal of Aerospace System Engineering
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• v.16 no.1
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• pp.17-27
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• 2022

A certain level of stability margin airworthiness criteria should be met to secure robustness against uncertainties between the real plant and the model in a flight control system design. The U.S. Department of Defense (DoD) specification of MIL-F-9490D and airworthiness certification standard of MIL-HDBK-516B uses gain and phase margin criteria of flight control system. However, the same stability margin criteria is applied at all development phases without considering the design maturity of each development phase of the aircraft. Ultimately, a problem arises when the aircraft operation envelope is excessively restricted. This paper proposes the relation of handling qualities and stability margin, and presents re-established stability margin criteria as a development phases and verification methods. The results of the research study are considered to contribute to the verification of the stability margin criteria more flexibly and effectively by applying the method to not only the currently manned developing aircrafts but also the unmanned vehicle to be developed in the future.

• Asia-pacific Journal of Multimedia Services Convergent with Art, Humanities, and Sociology
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• v.7 no.2
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• pp.427-435
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• 2017

Most of the domestic city buses are equipped with the pressure vessels subjected to internal pressure applied by compressed natural gas. Pressure vessels subjected to internal pressure are used in various forms and purposes. Fuel is explosive and has flammable high pressure. The damage of the pressure vessel causes many property damage and loss of life. Safe design for pressure vessel is always necessary. Due to these reasons, many studies using finite element analysis have been conducted. In this paper, the stresses of cylindrical vessel and spherical dome were analyzed using ANSYS, a finite element analysis software. In order to verify the validity of the analysis, a model with a perfectly spherical shape of the dome was designed and observed. Based on the ASME standard in used, stress distribution was also analyzed for models designed with compressed natural gas(CNG). The FEM analysis software agreed with the theory when the dome shape was perfectly spherical. The model designed based on the ASME specification theory, stress concentration occurred in the knuckle part.