• Title/Summary/Keyword: specific yield

Search Result 1,086, Processing Time 0.027 seconds

Ethanol Production Using Alginate Immobilized Cells of Zymomonas rnobilis (고정화 Zymomonas mobilis 균체로부터 에탄올 생산)

  • 한면수;정동효
    • Microbiology and Biotechnology Letters
    • /
    • v.20 no.5
    • /
    • pp.588-596
    • /
    • 1992
  • The fermentation characteristics of ethanol production by the use of immobilized Zymomonas mobilis KCTC 1534 cells were investigated in terms of formation factors such as substrate and product concentration. In batch fermentation, the maximum values of specific ethanol productivity, specific substrate uptake rate, ethanol yield, and glucose conversion rate were $29.14g/{\ell}{\cdot}h$, $60.24g/{\ell}{\cdot}h$, 0.48g/g, and 98.4%, respectively, with 17% glucose medium, and its ethanol productivity was $2.91g/{\ell}{\cdot}h$ in the case of 25 hour fermentation time. Repeated batch fermentation was possible for 30 days with 2.24-$2.94g/{\ell}{\cdot}h$ ethanol productivity. In semicontinuous fermentation, the maximum ethanol productivity was shown to be $15.7g/{\ell}{\cdot}h$ at $0.36h^{-1}$ effective dilution rate with 17% glucose concentration. In this case, ethanol yield coefficient and glucose conversion rate were 0.39 g/g, 64.7%, respectively.

  • PDF

Purification and Crystallization of the Recombinant Catalytic Subunit of Pyruvate Dehydrogenase Phosphatase (Pyruvate Dehydrogenase Phosphatase의 Catalytic Subunit의 분리정제 및 결정화)

  • Kim, Young-Mi
    • Journal of Food Hygiene and Safety
    • /
    • v.18 no.3
    • /
    • pp.146-152
    • /
    • 2003
  • Pyruvate dehydrogenase phosphatase (PDP) is a mitochondrial protein serine/threonine phosphatase that catalyzes the dephosphorylation and concomitant reactivation of the pyruvate dehydrogenase component of the pyruvate dehydrogenase complex (PDC). PDP consists of a catalytic subunit (PDPc, Mr 52,600) and regulatory subunit (PDPr, Mr 95,600). In the presence of $Ca^{2+}$, PDPc binds to the dihydrolipoamide acetyltransferase (E2) component of the pyruvate dehydrogenase complex in proximity to its substrate, the phosphorylated E1 component, thereby increasing the rate of dephosphorylation. PDPc possesses and intrinsic $Ca^{2+}$ binding site and a second $Ca^{2+}$ site is generated in the presence of E2. Using the unique interaction, highly pure PDPc was produced by the GSH-Sepharose-GST-L2 matrix with a specific activity of approx. 1000 U/mg and a yield of about 80%.

Microbial Community Diversity in Anaerobic Reactors Digesting Turkey, Chicken, and Swine Wastes

  • Ziganshina, Elvira E.;Belostotskiy, Dmitry E.;Shushlyaev, Roman V.;Miluykov, Vasili A.;Vankov, Petr Y.;Ziganshin, Ayrat M.
    • Journal of Microbiology and Biotechnology
    • /
    • v.24 no.11
    • /
    • pp.1464-1472
    • /
    • 2014
  • The microbial community structures of two continuous stirred tank reactors digesting turkey manure with pine wood shavings as well as chicken and swine manure were investigated. The reactor fed with chicken/swine wastes displayed the highest organic acids concentration (up to 15.2 g/l) and ammonia concentration (up to 3.7 g/l ammonium nitrogen) and generated a higher biogas yield (up to $366ml/g_{VS}$) compared with the reactor supplied with turkey wastes (1.5-1.8 g/l of organic acids and 1.6-1.7 g/l of ammonium levels; biogas yield was up to $195ml/g_{VS}$). The microbial community diversity was assessed using both sequencing and profiling terminal restriction fragment length polymorphisms of 16S rRNA genes. Additionally, methanogens were analyzed using methyl coenzyme M reductase alpha subunit (mcrA) genes. The bacterial community was dominated by members of unclassified Clostridiales with the prevalence of specific clostridial phylotypes in each reactor, indicating the effect of the substrate type on the community structure. Of the methanogenic archaea, methanogens of the genus Methanosarcina were found in high proportions in both reactors with specific methanosarcinas in each reactor, whereas the strict hydrogenotrophic methanogens of Methanoculleus sp. were found at significant levels only in the reactor fed with chicken/swine manure (based on the analyses of 16S rRNA gene). This suggests that among methanogenic archaea, Methanosarcina species which have different metabolic capabilities, including aceticlastic and hydrogenotrophic methanogenesis, were mainly involved in anaerobic digestion of turkey wastes.

Characterization of LaCrO$_3$ Powders Synthesized by Combustion Process with Different Heating Methods (가열방법에 따른 LaCrO$_3$ 연소합성분말의 특성)

  • 정층환;박홍규;오석진;박지연
    • Journal of the Korean Ceramic Society
    • /
    • v.35 no.10
    • /
    • pp.1078-1084
    • /
    • 1998
  • lanthanum chromite(LaCrO3) powder was synthesized by the combustion of a solution of metal nitrates La(NO3).6H2O and Cr(NO3)3.9H2O and urea. The pH of solution affected a yield of the combustion products but did not influence the morphology of the products. When the pH of the solution was in the range of 0.7-4, the yield of the combustion products was more than 90% The morphology of the combustion products was af-fected by heating methods for the solution. The hot-plate induced heating of the solution yielded powders hav-ing two-dimensionally interconnected agglomerates whereas microwave-induced heating produced a fine and non-agglomerated powders. The specific surface area(BET) of the combustion products using microwave-in-non-agglomerated powders. The specific surface area(BET) of the combustion products using microwave-in-duced heating method were larger(25~32m2/g) than that of hot plate heating method(10-14m2/g)

  • PDF

Nondestructive Nutrient Diagnosis for Nitrogen with Specific Color Difference Sensor(SCDS) in Hydroponics of Tomato (Lycopersicon esculentum MILL.) (토마토 양액 재배시 비파괴 간이 질소 영양 진단)

  • 이용범;노미영;조영렬;배종향
    • Journal of Bio-Environment Control
    • /
    • v.4 no.2
    • /
    • pp.175-180
    • /
    • 1995
  • This study was conducted to establish the nondestructive nutrient diagnosis method for nitrogen in tomato leaf using SCDS(specific color difference sensor). NFT(nutrient film technique) system was used in this experiment and nitrogen concentrations treated in nutrient solution were 0, 10, 50, 100, 150, 200, 300 and 600ppm. As nitrogen concentration in nutrient solution was increased from 0ppm to 150ppm, the stomatal resistance of tomato leaf was decreased abruptly, the $CO_2$ assimilation rate was increased but there was no big difference in the range of 100-500ppm. As the SCDS value of tomato leaf was increased, the $CO_2$ assimilation rate was increased linearly but the total average fruit weight and marketable yield were increased quadratically. The $CO_2$ assimilation rate was largely increased in the 0-3% range of leaf nitrogen content, but photosynthetic saturation was shown in 3.3-3.5%. The leaf nitrogen content was closely related to SCDS value of tomato leaf. Considering physiological activity, growth and yield of tomato, the optimum ranges of leaf nitrogen content were found to be 3.0-3.8% and the SCDS values equivalent for those ranges were 40.0-52.2.

  • PDF

Effects of the Site-Specific Nitrogen Management on Economic Feasibility and Environmental Sustainability (토양특성(土壤特性)에 따른 질소시용(窒素施用)의 환경(環境) 경제적(經濟的) 효과(效果))

  • Kang, Choong-Kwan;Park, Joo-Sub;Lee, Sang-Yong;Kim, Han-Myeong
    • Korean Journal of Soil Science and Fertilizer
    • /
    • v.34 no.1
    • /
    • pp.42-54
    • /
    • 2001
  • The use of nitrogen fertilizer in intensive agricultural production is of major concern due to its role on agricultural productivity and water quality. Crop production inputs on farm are usually applied at a uniform rate across an entire field. However, actual input requirements often vary within fields. The field variations in yield potential, soil moisture, soil N status, and the efficiency of fertilizer use, uniform application of crop production inputs does not allow optimum efficiency or profitability. This occurs because uniform application often results in areas of over- and under-application which may affect water quality and crop yield. This study used biophysical and economic models to assess the economic feasibility and water quality benefits of site specific nitrogen management for 10 soil types and 35 sample fields in Goodwater Creek watershed located near Centralia, Missouri. Results showed that the economic feasibility and water quality benefits of variable rate application were sensitive to the distribution of soil types within a field. Variable rate(VR) application was not uniformly more profitable than uniform rate(UR) application for the four agricultural systems evaluated and the water quality benefits were insubstantial relative to uniform application of N.

  • PDF

Comparison of Biological Nutrient Removal Efficiencies on the Different Types of Membrane (분리막 종류에 따른 하수의 생물학적 고도처리 효율 비교 연구)

  • Park, Jong-Bu;Shin, Kyung-Sook;Hur, Hyung-Woo;Kang, Ho
    • Journal of Korean Society on Water Environment
    • /
    • v.27 no.3
    • /
    • pp.322-328
    • /
    • 2011
  • This study was performed to investigate the characteristics of nutrient removal of municipal wastewater in the membrane bioreactor system with the different types of membrane. Membrane bioreactor consists of three reactors such as two intermittent anaerobic and the submerged membrane aerobic reactor with flat sheet and hollow fiber membrane, respectively. The removal efficiencies of $COD_{cr}$, BOD, SS, TN and TP on the flat sheet membrane bioreactor were 94.3%, 99.0%, 99.9%, 70.3% and 63.1%, respectively. In addition, The removal efficiencies of $COD_{cr}$, BOD, SS, TN and TP on the hollow fiber membrane bioreactor were 94.0%, 99.3%, 99.9%, 69.9% and 66.9%, respectively. The estimated true biomass yield, specific denitrification rate (SDNR), specific nitrification rate (SNR) and phosphorus removal content on the flat sheet membrane bioreactor were $0.33kgVSS/kgBOD{\cdot}d$, $0.043mgNO_3-N/mgVSS{\cdot}d$, $0.031mgNH_4-N/mgVSS{\cdot}d$, and 0.144 kgP/d, respectively. In addition, the estimated true biomass yield, specific denitrification rate (SDNR), specific nitrification rate (SNR) and phosphorus removal content on the hollow fiber membrane bioreactor were $0.30kgVSS/kgBOD{\cdot}d$, $0.067mgNO_3-N/mgVSS{\cdot}d$, $0.028mgNH_4-N/mgVSS{\cdot}d$, and 0.121 kgP/d, respectively. There was little difference between the flat sheet and hollow fiber on the nutrient removal efficiencies except SNR and SDNR. These differences between them were caused by the air demand to prevent the membrane fouling. The flux and oxygen demand for air scouring were $19.0L/m^2/hr$ and $2.28m^3/min$ for the flat sheet membrane, and $20.7L/m^2/hr$ and $1.77m^3/min$ for the hollow fiber membrane on an average.

Effect of Ridging System and Mulch Types on Growth, Yield, and Profitability of Potato (Solanum tuberosum L.) in Spring Cropping (감자(Solanum tuberosum L.) 봄재배 시 작휴와 멀칭이 생육, 수량 및 경제성에 미치는 영향)

  • Im, Ju Sung;Cho, Ji Hong;Cho, Kwang Soo;Chang, Dong Chil;Jin, Yong Ik;Yu, Hong Seob;Lee, Jong Nam
    • Korean Journal of Environmental Agriculture
    • /
    • v.35 no.3
    • /
    • pp.202-210
    • /
    • 2016
  • BACKGROUND: This study was conducted to determine effects of ridging systems and plastic film mulch types on growth, yield, and profitability of potato (Solanum tuberosum L.) in spring cropping using paddy field and to suggest profitable ridging system and plastic mulch type.METHODS AND RESULTS: Two potato cultivars ('Goun', and 'Atlantic') were grown at paddy field located in Gangneung in spring. For treatments, ridging systems were one-row ridge (OR) and two-row ridge (TR). Mulch types were no-mulch (NM), black plastic film mulch (BPM), and transparent plastic film mulch (TPM). Emergence of sprout was affected by the mulch types and the fastest level was shown at TPM treatment. Accumulative soil temperature during sprouting was 16.2℃ higher at TR than at OR and also higher at TPM than at BPM or NM. Stem length was not affected by ridging systems or mulch types. Leaf area index (LAI) was influenced by mulch types, while not by ridging systems. The highest LAI was shown at TR with TPM and OR of BPM. There was no significant difference in specific gravity or dry matter rate by ridging systems and mulch types. Tuber yield was significantly influenced by ridging systems and mulch types. Mean tuber weight was heavier at OR treatment than at TR and also the heaviest at TPM among mulch types. The highest marketable yield was found at OR with BPM. Based on marketable tuber yield and market price, the highest income ratio in two cultivars was found at OR with BPM and it was 20~82% higher than the ratio at TR with TPM.CONCLUSION: In spring potato cropping using paddy field, OR with BPM is better for high yield and is more profitable for farmer's income than the conventional cultivation method, TR with TPM.

Growth and Yield Characteristics of Rice Varieties Developed in Different Years (육성년도가 다른 벼 품종의 생육 및 수량특성)

  • 임준택;신동영;김학진
    • KOREAN JOURNAL OF CROP SCIENCE
    • /
    • v.38 no.4
    • /
    • pp.343-349
    • /
    • 1993
  • This study was conducted 1)to determine the factors responsible for the gradually increased rice yield by examining any improvements in yield-related characters and physiological characters among the historically recommended varieties of rice in Korea, and 2)to reveal the way of achieving further yield improvement by breeding in the future. Eight recommended varieties from 1911 to 1988 were selected and grown at the same conditions. The yield-related characters were observed at the harvest time, and the physiological characters such as RGR, NAR, LAR, SLA and L WR were estimated by the classical method of growth analysis. The newer varieties are shorter and those released after 1970's are greater in percent of filled spikelets and harvest index than the older varieties. There are no definite changing trends in the variations of number of panicles per hill, number of spikelets per panicle and 1000 grain weight according to the course of evolution in the recommended varieties. There is little evidence of improvement in the physiological characters such as NAR, LAR, SLA and LWR among the recently developed varieties compared with those of the older varieties. The increase in grain yield due to variety improvement, if any, is largely associated with the greater harvest index and percent of filled spikelets by breeding blight resistant and / or lodging resistant genotypes with short culm. It is suggested that increase in NAR should be carried out simultaneously with increase in harvest index to breed high yielding genotypes. I t appears to be important to breed genotypes with long culm in order to enhance light penetration into the canopy as long as they are lodging resistant. Since NAR is negatively correlated with LAR, it is unlikely to succeed to improve both characters simultaneously. The direct effect of NAR on RGR by path analysis is much larger than that of LAR, and hence it is suggested that breeding genotype with large NAR is more effective on enhancing RGR. It is also suggested that improving LAR through LWR is likely effective on increasing RGR.

  • PDF

Yield and Morphology of White Clover in Response to Infrequent, Frequent Defoliation and Their Alternations (예취빈도의 전환이 White Clover의 수량 및 형태적 특성에 미치는 영향)

  • 강진호;박진서
    • KOREAN JOURNAL OF CROP SCIENCE
    • /
    • v.40 no.2
    • /
    • pp.221-227
    • /
    • 1995
  • White clover (Trifolium repens L) gives rise to either weak persistence or overdominance in the pastures. To get information on grazing method to surmount the problem, the experiment was done to measure the effect of infrequent, frequent and their alternations on harvest yield and morphological characteristics of the clover. Individual plants of Regal, Louisiana S-l, Grasslands Huia, and Aberystwyth S184 were grown in 22cm plastic pot containing a 2: 1: 1 soil: sand: Peat moss mixture for 27 days after transplanting 50-day seedlings raised on 3cm pots, and then their all fully expanded leaves are trimmed. Defoliation treatments were forced every 1 (CC, frequent), 4 week(RR,infrequent) or their alternations(CR, RC) after 8 weeks from the trimming. To analyze the treatment effects, plants were sampled on 4, 8, 12, and 16 weeks after the trimming. Harvest yield of infrequent defoliation (RR) was higher than that of frequent defoliation (CC). Leaf area and no. of leaves per plant, petiole length and stolon length per plant, moreover, showed the similar result to the yield but stolon length and leaf area per g were reverse. The alternation of infre-quent and then frequent defoliation (RC) had greater yield than that of their reverse (CR) although both alternations showed intermediate ones compared to CC and RR. Morphological characteristics, furthermore, related to the clover leaf were immediately changed by alternations of defoliation inter-val(RC and CR) while those done to the stolon were lasted longer thereafter(RC and CR). Harvest yields in RC and CR were positively correlated to leaf area and no. of leaves or stolon length per plant but negatively done to leaf area and stolon length per g. It is concluded that weak persistence or overdominance of white clover in pasture can be controlled by alternation of infrequent and then frequent defoliation or reverse.

  • PDF