• Korean Journal of Pharmacognosy
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• v.42 no.2
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• pp.175-181
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• 2011

Oxidative stress or accumulation of reactive oxygen species (ROS) leads neuronal cellular death and dysfunction, and it contributes to neuronal degenerative disease such as Alzheimer's disease, Parkinson's disease and stroke. Glutamate is one of the major excitatory neurotransmitter in the central nervous system (CNS). Glutamate contributes to fast synaptic transmission, neuronal plasticity, outgrowth and survival, behavior, learning and memory. In spite of these physiological functions, high concentration of glutamate causes neuronal cell damage, acute insults and chronic neuronal neurodegenerative diseases. Heme oxygenase-1 (HO-1) enzyme plays an important role of cellular antioxidant system against oxidant injury. NNMBS020, the water-insoluble fraction of the 70% EtOH extract of root barks of Dictamnus dasycarpus, showed dominant neuroprotective effects on glutamate-induced neurotoxicity in mouse hippocampal HT22 cells by induced the expression of HO-1 and increased HO activity. In mouse hippocampal HT22 cells, NNMBS020 makes the nuclear accumulation of Nrf2 and stimulates extracellular signal-regulated kinase (ERK) pathway. The ERK MAPK pathway inhibitor significantly reduced NNMBS020-induced HO-1 expression, whereas the JNK and p38 inhibitors did not. In conclusion, the water-insoluble fraction of the 70% EtOH extract of root barks of D. dasycarpus (NNMBS020) significantly protect glutamate-induced oxidative damage by induction of HO-1 via Nrf2 and ERK pathway in mouse hippocampal HT22 cells.

• Development and Reproduction
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• v.18 no.4
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• pp.267-274
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• 2014

The immune system in teleost fish is not completely developed during embryonic and larval stages, therefore effective innate mechanisms is very important for survival in such an environment. However, the knowledge of the development of immune system assumed to be restricted. In many species, lysozymes have been considered as important genes of the first line immune defense. The early detection of lysozyme mRNA in previous reports, led to the investigation of its presence in oocytes. As a result, c-type lysozyme mRNA transcripts were detected in unfertilized oocytes indicating maternal transfer. Therefore, we investigated the expression patterns of lysozymes in flounder, including the matured oocyte. In our results, c-type lysozyme mRNA was first detected in unfertilized oocyte stage, observed the significantly decreased until hatching stage, and was significantly increased after hatching stage. On the other hand, g-type lysozyme mRNA transcripts were first detected at late neurula stage, and the mRNA level was significantly increased after 20 dph. It may be suggest that maternally supplied mRNAs are selectively degraded prior to the activation of embryonic transcription. This study will be help in understanding the maturation and onset of humoral immunity during development of olive flounder immune system.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.41 no.4
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• pp.241-247
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• 2005

The aim of this study was to develop a trawl escapement device which can improve the survival rate of young fishes passed through trawl codend. The sea experiments according to the bar space of the device and bar materials were carried out to decide the bar space and device materials in Korean southern sea and off Cheju island. Stainless pipes and MC bars were used to test the efficiency of escapement device materials. Although escapement rates of MC bar for main species were a little higher compare with stainless pipe, the efficiency of device for stainless pipe was better than MC bar. Escapement devices of sort-K type and grid panel type were also compared. Escapement rates of redlip croaker(Pseudosciaena polyactis), blackthroat seaperch(Doederleinia berycoides) and finespotted flounder (Pleuronichthys cornutus) showed 61.7%, 2.0% and 2.7% on the sort-x type and 69.9%, 1.1% and 2.0% for the grid panel type but the difference was little. Fishes under 20mm head width and fishes which swimming speed is so slow compared with body length could not go through well the device. Escapement rates according to the bar space were estimated using grid panel type. The rates of redlip croaker(Pseudosciaena polyactis) for the bar space of 20mm, 25mm, 35mm were increased as 60.3%, 61.0% and 77.8%, and 9.8%, 16.4% and 45.6% for horse mackere1(Trachurus japonicus), respectively.

• Journal of Korean Medical classics
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• v.23 no.2
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• pp.63-87
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• 2010

Natural Medicine is a medical system that tries to find the cause and cure of the disease in the nature emphasizing on natural healing power of human body. The most important value of traditional medicine in east Asia lies on the fact that this medicine is a medical culture and natural medicine that was selected by instinct and human nature reflecting the best possible remedy by the wisdom of evolution, and views the living phenomenon based on relation and circulation. This medicine is a natural medicine that tries to recover and promote the natural healing power by vitality and is a system that cumulates various empirical information about unique and complicated life phenomenon as a legacy of evolution in the body and the disease. The understanding of variation and adaptation of human species should be based on thorough knowledge of origin, instinct and nature of human as well as knowledge of survival and adaptation of human and environment based on evolution. And through this insight, the prevention and treatment of modern diseases should be developed. In this regards, traditional medicine in east Asia should be highlighted again as a study on adaptation and harmony of penetrating the cultural history of mind which has led the history of biological body and change of society and culture, and the repository of practical wisdom and knowledge.

• The Korean Journal of Mycology
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• v.39 no.2
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• pp.141-144
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• 2011

Short-preservation of basidiomycetes is generally being conducted in slant tubes containing solid medium based on agar. In this study, we investigated the vitality of 28 species and 76 strains preserved on potato dextrose agar (PDA) at $4^{\circ}C$ for 2~7 years. The survival rates of the fungi were 82%, 86%, 94%, 96%, 94%, and 94% for seven, six, five, four, three, and two years old preservation, respectively. The volume of medium in Lentinula edodes showed decrease after 2 years preserved. The pH of preserved medium was 5.42 in 2007 (two years old), but it became nearly neutral as increasing preservation term.

• Molecules and Cells
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• v.43 no.7
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• pp.662-670
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• 2020

We have investigated the involvement of the pre-mRNA processing factor 4B (PRP4) kinase domain in mediating drug resistance. HCT116 cells were treated with curcumin, and apoptosis was assessed based on flow cytometry and the generation of reactive oxygen species (ROS). Cells were then transfected with PRP4 or pre-mRNA-processing-splicing factor 8 (PRP8), and drug resistance was analyzed both in vitro and in vivo. Furthermore, we deleted the kinase domain in PRP4 using Gateway™ technology. Curcumin induced cell death through the production of ROS and decreased the activation of survival signals, but PRP4 overexpression reversed the curcumin-induced oxidative stress and apoptosis. PRP8 failed to reverse the curcumin-induced apoptosis in the HCT116 colon cancer cell line. In xenograft mouse model experiments, curcumin effectively reduced tumour size whereas PRP4 conferred resistance to curcumin, which was evident from increasing tumour size, while PRP8 failed to regulate the curcumin action. PRP4 overexpression altered the morphology, rearranged the actin cytoskeleton, triggered epithelial-mesenchymal transition (EMT), and decreased the invasiveness of HCT116 cells. The loss of E-cadherin, a hallmark of EMT, was observed in HCT116 cells overexpressing PRP4. Moreover, we observed that the EMT-inducing potential of PRP4 was aborted after the deletion of its kinase domain. Collectively, our investigations suggest that the PRP4 kinase domain is responsible for promoting drug resistance to curcumin by inducing EMT. Further evaluation of PRP4-induced inhibition of cell death and PRP4 kinase domain interactions with various other proteins might lead to the development of novel approaches for overcoming drug resistance in patients with colon cancer.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.44 no.6
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• pp.709-716
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• 2011

To investigate the distribution and migrations of sea turtles occurring in Korean waters, an adult female green sea turtle Chelonia mydas was tagged with a satellite transmitter and released at Busan in October 2009. The confirmation was made for the first time that the released green sea turtle migrated back to Korea after visiting Jeju Island and Japan for approximately 9 months. The green sea turtle spent the winter in Jeju and Japan because of low water temperatures in the East Sea and South Sea during winter months, which were not suitable for turtle survival. Compared with an immature green sea turtle that was released at Jeju Island, the adult green showed a markedly different migration pattern, suggesting that juvenile and adult greens move differently because of their peculiar life cycle and their stage of maturity, in addition to responses to temperature changes. Additional satellite tracking with increased representation of species, sexes and sizes of turtles, and regions is required to gather information on the distribution and movements of sea turtles in Korean waters.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.44 no.6
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• pp.747-752
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• 2011

The parrotfish Oplegnathus fasciatus is a sub-tropical species that is difficult to culture during the winter in South Korea. As a result, a recirculating aquaculture system (RAS) was developed to rear parrotfish. This study investigated the effects of stocking density and dissolved oxygen (DO) concentration on the growth and hematology of the parrotfish in the RAS. The experimental stocking densities were 5 (SD05), 10 (SD10), 15 (SD15), and 20 kg/m3 (SD20) total body weight to tank water volume. As the stocking density increased, the mean weight gain, feed efficiency, and specific growth rate tended to decrease. However, SD10 and SD15 did not differ statistically from SD05 in feed efficiency (P>0.05). Although better growth was achieved in SD05, SD10 and SD15 appeared to be acceptable, practically and economically, in terms of feed efficiency. The experimental DO concentration ranges were 3-4 (DO3), 5-6 (DO5), and 7-8 mg/L (DO7). The mean weight gain and survival tended to increase with the DO concentration, but there were no differences among treatments (P>0.05). Although the feed efficiency did not differ among the treatments, the specific growth rates and daily feed intake increased with the DO concentration and were the highest in DO7 (P<0.05). The cortisol concentration was the highest in DO3 (P<0.05), while there was no difference between DO5 and DO7 (P>0.05).

• Journal of Aquaculture
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• v.10 no.1
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• pp.39-47
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• 1997

Induction of hybrid and allotriploid between female rainbow trout, oncorhynchus mykiss and male cherry salmon, O. masou were performed. Triploid rate in allotriploid was 92% and the survival rates of allotriploid exceeded that of their hybrid counterparts. Karyological studies revealed that the hybrid had exactly the same intermediate diploid karyotypers as that of two parental species, while allotriploid had two sets of the maternal haploid complement and one set of the paternal haploid complement. Hybrid revealed an intermediate size in celluar and nuclear size of erythrocyte, while allotriploid revealed larger cellular and nuclear size of erythrocytes than hybrid. The number of nucleolar organizer regions of hybrid were 1 or 2, while those of allotriploids were 1, 2 or 3. These results proved that allotriploidization can lead to increase cytogenetical characters compared to counterpart hybridization.

• Journal of Aquaculture
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• v.12 no.1
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• pp.25-30
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• 1999

This study was carried out to produce the seedling of the larvae and juveniles of pale chup, Zacco platypus (Cyprinidae) in terms of artificial hatching, feeding behavior and growth rates. The total lengths of the newly hatched larvae were 7.6~8.2mm (mean: 7.97mm). The hatched larvae rose to the surface 3 days after hatching. The larvae were fed Daphnia, rotifer, Artemia and powdered feed. Hatching experiments were conducted in jar incubators under the different water temperature conditions, $20~32^{\circ}C$. The highest hatching rate was at $26^{\circ}C$ after 47 hours. Jar incubators was the highest hatching rate (89%), and cage and Californian incubators were useful and relatively high hatching rates (>89%). The size of the larvae 50 days after hatching were 30.0mm (25.6~32.0mm). As the water temperatures was increased, the growth rate of the larvae was also increased. Especially, the growth rates were favorable over $26^{\circ}C$, but survival was the worst at $32^{\circ}C$. This species was quick to accept assorted feed after hatching. The assorted feeds for flounder and rockfish better than that of carp in reference to growth rate.