• Title/Summary/Keyword: species collection

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Molecular Discrimination of Mitis Group Streptococci Isolated from Koreans using RpoB Nucleotide Sequences

  • Park, Soon-Nang;Kook, Joong-Ki
    • International Journal of Oral Biology
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    • v.38 no.1
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    • pp.29-36
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    • 2013
  • Mitis group streptococci (MGS) were classified based on the nucleotide sequences 16S rRNA gene (16S rDNA) and comprised 13 Streptococcus species. However, 16S rDNA homogeneity among MGS was too high to discriminate between clinical strains at the species level, notably between Streptococcus mitis, Streptococcus oralis, Streptococcus pneumoniae, and Streptococcus pseudopneumoniae. The purpose of this study was to discriminate between 37 strains of MGS isolated from Korean oral cavities using phylogenetic analysis of the DNA-dependant RNA polymerase beta-subunit gene (rpoB). 16S rDNA and rpoB from clinical strains of MGS were sequenced using the dideoxy chain termination method and analyzed using MEGA version 5 software. The resulting phylogenetic data showed that the rpoB sequences could delineate clinical strains of MGS at the species level. Phylogenetic analysis of rpoB is therefore a useful approach for identifying MGS at the species level.

Master Plan for the Incheon Metropolitan City Arboretum (인천광역시 수목원 조성 기본계획)

  • Cho, Woo;Chang, Chong-Soo;Min, Seong-Hwan;Oh, Kang-Im
    • Journal of the Korean Institute of Landscape Architecture
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    • v.31 no.5
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    • pp.96-107
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    • 2003
  • This study is to establish the Incheon Metropolitan City Arboretum Development Plan (IMADP) as a capacity building both for plant diversity conservation and nature friendly leisure activity areas of Incheon Metropolitan City, Korea. The arboretum planning area is located within territory of the Incheon Grand Park Based on the IMADP, the arboretum was divided into three display spaces; the Urban Greening Garden, the Rare and Endangered Plant Species Garden and the Theme Garden. Detailed planting guidelines were suggested according to the three garden areas. The Urban Greening Garden was designed to display the following three; the relevance techniques, the model, and the practical uses of plants that are regarded to an urban greening. The Rare and Endangered Plant Species Garden (REPSG) was planned for the collection and display of the rare and endangered native plants in the territories of Incheon and the island seashore. Also, the REPSG includes the education purposes of comparing and displaying taxonomically related plant species. The Theme Garden was projected for the collection and display of plant species that have long been familiar to Korean culture and which are also used in our daily life.

DNA Fingerprinting of Jute Germplasm by RAPD

  • Hossain, Mohammad Belayat;Haque, Samiul;Khan, Haseena
    • BMB Reports
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    • v.35 no.4
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    • pp.414-419
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    • 2002
  • The genotype characteristic of cultivars was investigated, along with varieties of both of the jute species, Corchorus olitorius and Corchorus capsularis, in the germplasm collection at the Bangladesh Jute Research Institute (BJRI). DNA fingerprinting was generated for 9 different varieties and 12 accessions of jute cultivars by using random amplified polymorphic DNA(RAPD). A total of 29 arbitrary oligonucleotide primers were screened. Seven primers gave polymorphism within the varieties, and 6 primers detected polymorphism within the accessions that were tested. A dendrogram was engendered from these data, and this gave a distinct clustering of the cultivated species of jute. Therefore, we generated RAPD markers, which are species-specific. These primers can distinguish between C. olitorius and C. capsularis. From the dendrogram that we generated between the various members of these two species, we found the existing genetic classification that agrees with our molecular marking data. A different dendrogram showed that jute accessions could be clustered into three groups. These data will be invaluable in the conservation and utilization of the genetic pool in the germplasm collection.

Changes of Morphological and Growth Characteristics Collected Miscanthus Germplasm in Korea (국내 억새 유전자원 수집 후 형태 및 생육 특성 변화)

  • Song, Yeon-Sang;Lee, Ji-Eun;Moon, Youn-Ho;Yu, Gyeong-Dan;Choi, In-Seong;Cha, Young-Lok;Kim, Kwang-Soo
    • Weed & Turfgrass Science
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    • v.7 no.1
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    • pp.22-34
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    • 2018
  • Miscanthus has been considered as the most promising bioenergy crop for lignocellulosic biomass production. In Korea, M. sacchariflorus and M. sinensis can be found easily in all regions. It is a great advantage to utilize as important species with respect to genetic and cross-breeding programs materials for creation of novel hybrids. For successful breeding programs, it is important to precisely understand the variability of morphological and growth characteristics among Miscanthus species as breeding parent materials. In this study, morphological and growth characteristics were observed in 960 germplasms of two Miscanthus species (M. sacchariflorus and M. sinensis) for growing seasons over three years. Due to the inherent characteristics of these species, the germplasm of M. sacchariflorus among the collected germplasm were reduced in plant height than in the collection area. In M. sinensis, the plant height of germplasm collected mainly from Jeju-do increased more than those collected from collection area. Sixty-one of the collected 960 germplasms were selected and investigated to the morphological characteristics. Based on the investigated morphological data, the phylogenic tree was developed. As the results, it was confirmed that there exist germplasm in which the characteristics of M. sacchariflorus and M. sinensis are mixed. This study of Miscanthus may provide an important information in order to expedite the introduction as breeding materials for creation of new hybrid.

Species Diversity of Hypogeous Ascomycetes in Israel

  • Barseghyan, Gayane S.;Wasser, Solomon P.
    • Mycobiology
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    • v.38 no.3
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    • pp.159-165
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    • 2010
  • We conducted a species diversity study of the hypogeous Ascomycetes of Israel. The hypogeous Ascomycetes in Israel include members of the families Pyronemataceae, Pezizaceae, and Tuberaceae, which are represented by seven species: Hydnocystis piligera, Terfezia arenaria, T. claveryi, T. oligosperma, Tirmania africana, Tuber asa, and T. nitidum; only T. asa is new to Israeli mycobiota. Synonymy, locations, collection data, general distribution, distribution in Israel, descriptions, a key to identification, illustrations, and taxonomic remarks are provided.

Taxonomical Characteristics of Tropical Species of Xylaria Collected from Taiwan (대만산 Xylaria속 열대종의 분류학적 특성)

  • Lee, Yang-Soo
    • The Korean Journal of Mycology
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    • v.27 no.1 s.88
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    • pp.73-75
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    • 1999
  • Three species of the genus Xylaria are recognized on Taiwan materials: X. allantoidea (Berk.) Fr. and X. obovata (Berk.) Fr.; including one new record X. curta Fr., having $9.0-10.8{\times}3.9-5.4\;{\mu}m$ ascospores, in Taiwan. The collection of Xylaria allantoidea from Taiwan is compared to other collections in morphological characters. The species are described from the host and in culture by a bright microscope and a scanning electron microscope.

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Eight Taxa of Newly Recorded Species of Chlorophytes (Chlorophyceae and Trebouxiophyceae, Chlorophyta) in Korea

  • Kim, Mi Ran;Kim, Jee Hwan;Kim, Do Hyun;Lee, Ok Min
    • Korean Journal of Environmental Biology
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    • v.36 no.3
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    • pp.277-284
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    • 2018
  • In 2017, the freshwater algae were collected from reservoirs, small ponds, soil, and rocks in Korea. Eight taxa of Chlorophyta (Chlorophyceae and Trebouxiophyceae) have been newly reported in Korea. The unrecorded indigenous species were Chlorolobion braunii, Coelastrum pseudomicroporum, Coelastrum reticulatum var. cubanum, Monoraphidium nanum, Tetrachlorella incerta, Ecdysichlamys obliqua, Gloeotila scopulina, and Stichococcus jenerensis.

Checklist of Mosquitoes (Culicidae) in Korea (한국산 모기과(Culicidae)의 목록)

  • 이관우
    • Parasites, Hosts and Diseases
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    • v.25 no.2
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    • pp.207-209
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    • 1987
  • A systematic mosquito taxonomic study in Korea was established by Lee in 1971. Since then, many species have been recorded as new to Korea. However, much of this taxonomic information has been distributed in a number of scientific publications. This fork attempts to combine this information into a single document, as well as to synthesize revised taxonomic synonyms of species cccurring in Korea. Four species, Culex vora.x, Culex kangi, Aedes kobayashii and Culiseta kanayamensis listed in our previous paper, are synonyms of Culex kalifaxii, Cutex jackseni, Aedes alehtorowi and Culiseta bergrothi, respectively. Culex annulus is a misidentified species of Culex pseudovishnui. The subspecies Cutes pipiens quinquefassiatus and subgenus Manscnia (Ccquillettidia) were treated as full species and genus respectively in a catalog of the mosquitoes of the world. However, not all current authors agree to those specific and generic treatment. Based on available publications the total number of mosquito species recorded in Korea is now 52 representing 9 genera. Forty-five of these 52 are confirmed species with preserved specimen representation. As collection of specimen continues, there is a possibility that representative of 7 unconfirmed species will be found. 1 hope this paper will provide useful information for entomology workers who are interested in mosquito taxonomy in Korea.

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Unrecorded species of Korean protozoans discovered through the project of 'Discovery of Korean Indigenous Species' III

  • Kyu-Seok Chae;Kang-San Kim;Jongwoo Jung;Gi-Sik Min
    • Journal of Species Research
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    • v.12 no.4
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    • pp.355-361
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    • 2023
  • In this paper, we present unrecorded protozoans of Korea that were discovered, through the 'Discovery of Korean Indigenous Species' project hosted by the 'National Institute of Biological Resources (NIBR)'. A taxonomic account is provided for each identified species, offering comprehensive information such as species name, Korean name, collection site, synonyms, specimen vouchers, diagnoses, and figures. This study introduces 13 previously unrecorded Korean protozoan species that are classified into three phyla: Amoebozoa, Cercozoa, and Ciliophora. Notably, the cercozoan family Chlamydophryidae was recorded in Korea for the first time, together with the discovery of three previously unreported genera: Diaphoropodon within Cercozoa, and Metauroleptus and Hemicycliostyla within Ciliophora.

A Study on Protection Plan of Eutrophication in Fresh Water Environment by Development of Methods for Algal Growth Potential test (I) -Morphology and Growth Characteristics of Isolated algae- (조류생산잠재력조사 방법개발에 의한 육수환경의 부영양화 방지대책에 관한 연구(I) -순수분리종의 형태 및 증식특성-)

  • 위인선;나철호;이종빈;주현수
    • Journal of Environmental Health Sciences
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    • v.23 no.1
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    • pp.18-27
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    • 1997
  • The isolation, morphological study and growth characteristics of the algae were investigated from Lake Chuam. The isolated algae were applied the Agal Growth Potential test. The method of isolation and purification of the algae were used to Agar plating(AP), nutrient enrichment(NE), dilution(DI) and micro capillary technique(MC). Total isolated algae were 21 species. They were composed of Cyanophyceae, Dinophyceae, Bacillariophyceae, Euglenophyceae and Chlorophyceae. The numbers of algal strain by isolation technique were highest in dilution(21 species), and those of the rests were showed in order of NE > MC > AP. The sizes of isolated Selenastrum and Scenedesmus were $1.8\pm 1.4 \mu m$, $3.3\pm 0.9 \mu m$ in diameter and $6.4\pm 2.3 \mu m$, $13.6\pm 1.9 \mu m$ in length respectively. The morphology of isolated algae and NIES-collection strain was very similar each other, but the size was smaller isolated algae than that of NIES-collection. The optimum culture condition of isolated Selenastrum and Scenedesmus was about 30$\circ$C(25$\circ$C-35$\circ$C) in temperature and the maximum growth was appeared between 7,000 lux and 8,000 lux in the light intensity. The comparison of $\mu$(specific growth rate) on the concentration of nutrients such as nitrate and phosphate, isolated Selenastrum was appeared maximum it at 1.0 mg $NO_3-N/l$ but NIES-collection strain was showed 95% of maximum it at same nitrate concentration. Maximum g of isolated algae and NIES-collection strain in Scenedesmus onto nitrate concentration were very similar with the result of selenastrum. The specific growth rates of isolated algae and NIES-collection strain on the gradient concentration of phosphate were showed 0.72/day and 0.70/day at 0.02 mg $PO_4-P/l$ in Selenastrum but those of Scenedesmus were appeared 0.61/day and 0.57/day at same concentration $PO_4-P$.

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