• Journal of Microbiology
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• 제38권2호
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• pp.62-65
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• 2000

Partial sequence comparisons of mitochondrial small subunit rDNA (mt SSU rDNA) were used to examine taxonomic and evolutionary relationships among seven Penicillium species : two monoverticillate species, two biverticillate species, and three terverticillate species. Amplified fragments of mt SSU rDNA highly varied among seven species in size, suggesting the existence of multiple insertions or deletions in the region. A phylogengtic tree was constructed by exhaustive search of parsimony analysis. The phylogenetic tree distinguished two statistically supported monophyletic groups, one for two monoverticillate species and the other for three terverticillate species and ont biverticillate species, P. vulpinum. The phylogenetic relationship of P. waksmanii, the biverticillate species, was not clear.

• The Plant Pathology Journal
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• 제40권1호
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• pp.16-29
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• 2024

The Colletotrichum gloeosporioides species complex includes many phytopathogenic species, causing anthracnose disease on a wide range of host plants and appearing to be globally distributed. Seventy-one Colletotrichum isolates in the complex from different plants and geographic regions in Korea were preserved in the Korean Agricultural Culture Collection (KACC). Most of them had been identified based on hosts and morphological features, this could lead to inaccurate species names. Therefore, the KACC isolates were re-identified using DNA sequence analyses of six loci, comprising internal transcribed spacer, gapdh, chs-1, his3, act, and tub2 in this study. Based on the combined phylogenetic analysis, KACC strains were assigned to 12 known species and three new species candidates. The detected species are C. siamense (n = 20), C. fructicola (n = 19), C. gloeosporioides (n = 9), C. aenigma (n = 5), C. camelliae (n = 3), C. temperatum (n = 3), C. musae (n = 2), C. theobromicola (n = 2), C. viniferum (n = 2), C. alatae (n = 1), C. jiangxiense (n = 1), and C. yulongense (n = 1). Of these, C. jiangxiense, C. temperatum, C. theobromicola and C. yulongense are unrecorded species in Korea. Host plant comparisons showed that 27 fungus-host associations are newly reported in the country. However, plant-fungus interactions need to be investigated by pathogenicity tests.

• The Plant Pathology Journal
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• 제39권4호
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• pp.384-396
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• 2023

Colletotrichum acutatum species complex is one of the most important groups in the genus Colletotrichum with a high species diversity and a wide range of host plants. C. acutatum and related species have been collected from different plants and locations in Korea and deposited into the Korean Agricultural Culture Collection (KACC), National Institute of Agricultural Sciences since the 1990s. These fungal isolates were previously identified based mainly on morphological characteristics, and a limitation of molecular data was provided. To confirm the identification of species, 64 C. acutatum species complex isolates in KACC were used in this study for DNA sequence analyses of six loci: nuclear ribosomal internal transcribed spacers (ITS), betatubulin 2 (TUB2), histone-3 (HIS3), glyceraldehyde3-phosphate dehydrogenase (GAPDH), chitin synthase 1 (CHS-1), and actin (ACT). The molecular analysis revealed that they were identified in six different species of C. fioriniae (24 isolates), C. nymphaeae (21 isolates), C. scovillei (12 isolates), C. chrysanthemi (three isolates), C. lupini (two isolates), and C. godetiae (one isolate), and a novel species candidate. We compared the hosts of KACC isolates with "The List of Plant Diseases in Korea", previous reports in Korea and global reports and found that 23 combinations between hosts and pathogens could be newly reported in Korea after pathogenicity tests, and 12 of these have not been recorded in the world.

• Mycobiology
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• 제51권5호
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• pp.288-299
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• 2023

Aspergillus is one of the largest and diverse genera of fungi with huge economical, biotechnological, and social significance. Taxonomically, Aspergillus is divided into six subgenera comprising 27 sections. In this study, 235 strains of Aspergillus subgenus Circumdati (section: Candidi, Circumdati, Flavi, Flavipedes, Nigri, and Terrei) preserved at the Korean Agricultural Culture Collection (KACC) were analyzed and re-identified using a combined dataset of partial b-tubulin (BenA), Calmodulin (CaM) gene sequences and morphological data. We confirmed nineteen species to be priorly reported in Korea (A. neotritici, A. terreus, A. floccosus, A. allahabadii, A. steynii, A. westerdijkiae, A. ochraceus, A. ostianus, A. sclerotiorum, A. luchuensis, A. tubingensis, A. niger, A. welwitschiae, A. japonicus, A. nomius, A. tamarii, A. parasiticus, A. flavi, and A. oryzae). Among the studied strains, three species (A. subalbidus, A. iizukae, and A. uvarum), previously unreported or not officially documented, were discovered in Korea, to the best of our knowledge. We have given a detailed description of the characteristic features of the three species, which remain uncharted in Korea.

• 환경위생공학
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• 제17권1호
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• pp.1-11
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• 2002

The results of adult mosquitoes collection at Uijeongbu-si, Dongducheon-si in Gyeonggi-do where malaria outbreak occur in Korea are presented. Light traps collection were operated for adult collection from April to October, 2001. Among the total 12 species comprising 4 genera, 4 species in Anopheles, 3 species in Aedes, 1 species in Armigeres and 4 species in Culex were identified in the collection using the black light traps. The adult population densities of Anopheles sinensis peaked at an average of 200 in Gwangam-dong, Dongduchen-si in July and an average an average of 977 in Songsan-dong, Uijeongbu-si in August. Nocturnal Activities of Anopheles sinensisn peaked at between 22:00 ∼23:00(21.0%) and 16.4% between 21:00 and 22:00, 13.1% between 23:00 and 24:00, 13.0% between 20:00 and 21:00, 11.7% between 24:00 and 01:00 in averages.

• Journal of Microbiology and Biotechnology
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• 제10권3호
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• pp.363-366
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• 2000

The sequences of the D1/D2 region of 26S rDNA from seven Sporobolomyces species, Bensingtonia subrosea, and Rhodosporicium toruloides were determined and compared with those from representatives of the genera Leucosporidium, Rhodosporidium, Rhodotorula, and Sporidiobolus. The five species of Sporobolomyces analyzed were distantly related to a monophyletic clade consisting of species of Sporidiobolaceae and Sporobolomycetaceae. Sporobolomyces falcatus was found to be closely related to Tremella exigua. The members of Sporidiobolaceae and Sporobolomycetaceae were divided into four groups. Group 1 was composed of Leucosporidium scottii and two Rhodotorula species, and group 2 contained three Rhodotorula species. Group 3 was designeate as the Sporobolomyces/Sporidiobolus core group, as it contained Sporidiobolus johnsonii, the type species of Sporidiobolus and the teleomorphic state of Sporobolomyces salmonicolor (the type species of Sporobolomyces). Group 4, named the Rhodotorula/Rhodosporidium core group, included Rhodosporidium toruloides and Rhodotorula glutinis, the type species of the genera Fhodosporidium and Rhodotorula, respectively. The four groups were differentiated on the basis of their physiological characteristics including the assimilation of D-glucosamine, glucuronate, 2-keto-gluconate, L-arabinitol, raffinose, methyl-$\alpha$-glucoside, and satrch. The taxonomy of the genera Leucosporidium, Rhodosporidium, Rhodotorula, Sporidiobolus, and Sporobolomyces will require a major revision when more data becomes available.

• Journal of Microbiology and Biotechnology
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• 제24권3호
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• pp.334-336
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• 2014

A new species named Aspergillus cumulatus sp. nov. is described in Aspergillus section Aspergillus (Eurotium state). The type strain (KACC $47316^T$) of this species was isolated from rice straw used in meju fermentations in Korea, and other strains were isolated from the air in a meju fermentation room. The species is characterized by growth at a wide range of water activities and the formation of aerial hyphae on malt extract 60% sucrose agar (ME60S) that resemble a cumulus cloud. Furthermore, A. cumulatus produces yellow ascomata containing small lenticular ascospores (5.1-5.7 ${\mu}m$) with a wide furrow, low equatorial crests, and tuberculate convex surface. The species is phylogenetically distinct from the other reported Aspergillus section Aspergillus species based on multilocus sequence typing using rDNA-ITS, ${\beta}$-tubulin, calmodulin, and RNA polymerase II genes.

• Journal of Oral Medicine and Pain
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• 제47권1호
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• pp.10-26
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• 2022

Purpose: The purpose of this study was to investigate whether various saliva collection methods affect the observed salivary microbiome and whether microbiomes of stimulated and unstimulated saliva and plaque differ in richness and diversity. Methods: Seven sampling methods for unstimulated saliva, stimulated saliva, and plaque samples were applied to six orally and systemically healthy participants. Bacterial 16S ribosomal RNA genes of 10 major oral bacterial species, namely, Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, Fusobacterium nucleatum, Prevotella intermedia, Prevotella nigrescens, Streptococcus mitis, Streptococcus sobrinus, and Lactobacillus casei, were analyzed by real-time polymerase chain reaction. We comprehensively examined the dependence of the amount of bacterial ribosomal DNA (rDNA), bacterial-community composition, and relative abundance of each species on sample collection methods. Results: There were significant differences in the bacterial rDNA copy number depending on the collection method in three species: F. nucleatum, P. nigrescens, and S. mitis. The species with the highest richness was S. mitis, with the range from 89.31% to 100.00%, followed by F. nucleatum, P. nigrescens, T. denticola, T. forsythia, and P. intermedia, and the sum of the proportions of the remaining five species was less than 1%. The species with the lowest observed richness was P. gingivalis (<0.1%). The Shannon diversity index was the highest in unstimulated saliva collected with a funnel (4.449). The Shannon diversity index was higher in plaque samples (3.623) than in unstimulated (3.171) and stimulated (3.129) saliva and in mouthwash saliva samples (2.061). Conclusions: The oral microbial profile of saliva samples can be affected by sample collection methods, and saliva differs from plaque in the microbiome. An easy and rapid technique for saliva collection is desirable; however, observed microbial-community composition may more accurately reflect the actual microbiome when unstimulated saliva is assayed.

• 한국균학회지
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• 제44권4호
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• pp.207-219
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• 2016

인간의 삶에 큰 영향을 끼치는 Aspergillus, Penicillium 및 그들의 완전세대 속에 속하는 곰팡이의 국내 보고 종 목록을 작성하였다. 기존의 국가 생물종 목록집에는 Aspergillus 14종, Eurotium 4종, Neosartorya 8종, Penicillium 47종, Talaromyces 5종이 보고되었다. 본 논문에서는 국제조류 균류 식물명명규약(ICN)에 따른 일균일명 체계에 따라 Eurotium과 Neosartorya은 Aspergillus 속으로 합치고 Penicillium과 Talaromyces도 새로운 속 개념에 따라 정리하였다. 또한 기존의 국가 생물종 목록에 빠져 있던 77종을 추가하여 Aspergillus, Penicillium 및 그들의 완전세대에 속하는 곰팡이의 국내 보고 종 목록을 Aspergillus 55종, Penicillium 82종, Talaromyces 18종으로 정리하였다.

• 한국약용작물학회지
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• 제18권3호
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• pp.201-207
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• 2010

This study was carried out to obtain the information on the antioxidant activity of Phyllanthus. We compared and analyzed three indigenous collections of Phyllanthus: species from Korea, Brazil and Paraguay. Total phenolic acid contents were $1.93{\pm}0.015$ mg/mL in KCS (Korean Collection Species), $1.65{\pm}0.003$ mg/mL in PCS (Paraguay Collection Species) and $1.49{\pm}0.003$ mg/mL in BCS(Brazil Collection Species). In both DPPH radical scavenging activities and SOD-liked activities, KCS was the highest ($67.25{\pm}0.03%,\;78.38{\pm}1.17%$). KCS were also the highest in hydroxyl radical scavenging activities ($95.80{\pm}0.17%$). It turned out that there was no meaningful difference between BCS ($81.64{\pm}0.23%$) and PCS ($81.54{\pm}0.04%$). As for hydrogen radical scavenging activities, there was no statistically meaningful difference between KCS ($36.44{\pm}0.24%$) and BCS ($36.31{\pm}0.33%$) and it was the lowest in PCS ($33.72{\pm}0.15%$). Our results showed that KCS excelled the others not only in antioxidant activity but also in other beneficial effects. This encouraging results may be potentially useful in developing Phyllanthus urinaria L. variety which contains lots of functional elements such as total phenolic acid.