• 한국양식학회지
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• 제3권1호
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• pp.25-30
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• 1990

차넬메기(Ictalurus punctatus)는 전 세계적으로 중요한 양식어종이나 우리나라의 경우 대량산란이 이루어지지 않아 아직 양식 대상종으로 각광받지 못하고 있다. 본 연구는 차넬메기를 염색체공학 기법을 이용, 배수체를 생산하기 위한 일환으로 온도자극과 호르몬처리를 통해 산란을 유도하였다. 그 결과 처리하지 않은 군은 산란하지 않은 반면 온도자극시, $24^{\circ}C$로 부터 $30^{\circ}C$로 $6^{\circ}C$를 증가시킨 군이 $24^{\circ}C$ 부터 $27^{\circ}C$까지 온도를 증가시킨 군보다 약 4배 정도의 높은 산란유도율을 보였다. 본 실험에 사용된 호르몬 중 잉어뇌하수체를 어체중 1 kg당 4.4mg의 농토로 1$\~$3회 주사한 군은 $78.6\%$의 산란유도율을 보였고, human chorionic gonadotropin을 어체중 1kg당 1,100 IU의 농도로 1$\~$3회 주사한 군은 $66.6\%$의 산란유도율을 나타내었다.

• 한국수산과학회지
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• 제40권6호
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• pp.394-410
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• 2007

Anchovy spawn from the end of May to mid-October, when the water temperature is $14.8-27.2\;^{\circ}C$ and the salinity is 26.0-33.6 psu. The main spawning season is between July to August, when the water temperature is $21.7-27.2\;^{\circ}C$ and the salinity is between 26.0-32.2 psu. The main spawning grounds of anchovy are coastal areas shallower than 50 m around the islands located in the Jeju Strait. Anchovy larvae are distributed near the fronts between Chuja-do, Jangsu-do, Yeoseo-do, and the open sea rather than in the spawning grounds. Anchovy eggs and larvae density increased in accordance with the high level of $Chlorophyll-{\alpha}$ during the summer season (July-August). In terms of the suspended sediment (SS) levels along the northern coast of the Jeju Strait, high densities of anchovy eggs (12.0-18.0 mg/L) were observed, mainly in the area affected by the coastal waters of the southern sea with high SS levels, while larvae (10.0-19.0 mg/L) tended to be distributed over a wide area with high SS levels, including the open sea. In terms of the dissolved oxygen (DO) content, eggs (5.4-6.8 mg/L) were observed in coastal areas with a high DO content, while larvae (4.2-6.4 mg/L) were distributed widely in areas with a relatively low DO content, from the southern coast to the open sea.

• 한국수산과학회지
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• 제50권1호
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• pp.41-47
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• 2017

Perhaps the most common type of reproductive dysfunction in captive fish is failure of females to undergo final oocyte maturation and thus to ovulate and spawn. The success of aquaculture could therefore be improved by developing techniques to enhance natural spawning, artificial maturation, and/or to induce ovulation in farmed fish. This study aimed to investigate the effects of prostaglandin $E_2$ ($PGE_2$) and prostaglandin $F_{2{\alpha}}$ ($PGF_{2{\alpha}}$) on in vitro oocyte maturation (germinal vesicle breakdown, GVBD) and ovulation in the marine fish Chasmichthys dolichognathus. Post-vitellogenic follicles (0.80-0.94 mm diameter oocytes) were incubated with $PGE_2$ or $PGF_{2{\alpha}}$ at concentrations of 5, 50, or 500 ng/mL for 24 hours. A significant increase in GVBD was seen in 0.84 mm and 0.94 mm oocytes incubated with 50 ng/mL $PGE_2$ compared with the control. There was no significant increase in GVBD in any of the other experimental conditions (5 or 500 ng/mL $PGE_2$ or 5, 50, or 500 ng/mL $PGF_{2{\alpha}}$). Neither of the prostaglandins induced ovulation at the concentrations tested.These results suggest that GVBD was induced by incubation with 50 ng/mL $PGE_2$.

• 한국균학회지
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• 제28권1호
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• pp.6-10
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• 2000

마른진흙버섯(Phellinus gilvs)은 진흙버섯속(Phellinus)에 속하는 버섯으로 본초도감에서는 비장강화, 위장활성 등의 약효가 있다고 한다. 온도별 배양결과 $30^{\circ}C$에서 69 mm/14일(PDA배지) $25^{\circ}C$에서는 42 mm/14일(PDA배지)로 나타났으며 배지별 시험에서는 PDA 배지와 MCM 배지에서 38 mm/14일, 34 mm/14일로 각각 나타났으며 $pH\;6{\sim}7$에서 균사생장이 가장 양호하였다. 참나무추출물배지에서는 24 mm/14일 정도였으나 소나무추출물배지에서는 2.7 mm/14일 정도로 참나무추출물배지에 비해서 매우 느렸다. 시험관을 이용한 톱밥배지 배양에서는 참나무톱밥+버드나무톱밥(5:5)과 참나무톱밥+버드나무톱밥+미강(4.5:4.5:1) 등에서 균사생장이 좋았다. 참나무원목에 접종한 결과 접종 60일 경과시 원목내 균사생장은 200 mm로 나타났으며 균사활착율은 100%였고 최초 발이 소요기간은 접종 후 90일 정도였다. 자실체 수량은 참나무원목에서 180 g, 소나무원목에서 29 g, 사과나무에서는 150 g 정도로 나타났으며 생체수율은 각각 24%, 9%, 15% 정도였다.

• 한국균학회지
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• 제28권1호
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• pp.11-15
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• 2000

Phellinus pini는 진흙버섯속(Phellinus)에 속하는 버섯으로 진흙버섯속 버섯 중에서 항암저지율이 가장 높다고 알려져 있다(중국본초도감). 온도별 배양결과 $25^{\circ}C$에서 34 mm/14일(PDA배지)로 생육이 가장 좋았고 배지별 시험에서는 PDA 배지에서 30mm/10일, MCM 배지에서 28mm/10일로 나타났으며 pH $5{\sim}6$에서 균사생장이 양호하였다. 시험관을 이용한 톱밥배지 배양에서는 참나무톱밥+버드나무톱밥 +미강(4.5:4.5:1)과 참나무톱밥+소나무톱밥+미강(4.5:4.5:1)에서 균사생장이 가장 빨랐고 균사밀도는 참나무톱밥+미강(9:1)과 소나무톱밥+미강(9:1) 배지가 가장 높았다. 참나무원목에 접종한 결과 접종 60일 경과시 원목내 균사생장은 40 mm로 나타났으며 균사활착율은 26%정도였고 최초 발이 소요기간은 접종 후 110일 정도로 나타났다.

• Mycobiology
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• 제40권3호
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• pp.164-167
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• 2012

The effects of aeration through lid filters on the hyphal growth of Lentinula edodes (oak mushroom) in sawdust cultivation bags were investigated. The aeration treatment levels were traditional 27 mm hole cotton plugs, cotton balls and combinations of seven hole sizes ${\times}$ two hole positions (up and under) in the lids covering plastic bags containing 1.4 kg sawdust medium at 63% moisture that had been autoclaved for one hour and inoculated with sawdust spawn of L. edodes strain 921. Aeration treatment effects were measured based on the $CO_2$ concentration at the 15th wk, as well as the hyphal growth rate and degree of weight loss of bags every 14 days for 15 wk. In bags with traditional cotton plugs, the $CO_2$ concentration was $3.8{\pm}1.3%$, daily mean hyphal growth was $2.3{\pm}0.6mm$ and daily mean weight loss was $0.84{\pm}0.26g$. In the bags with 15 mm diameter holes, the $CO_2$ concentration was $6.0{\pm}1.6%$, daily hyphal growth was $2.8{\pm}0.2mm$ and daily weight loss was $0.86{\pm}0.4g$. The bags with 15 mm holes had a higher $CO_2$ concentration and lower water loss than bags with other hole sizes, but the hyphal growth was not significantly different from that of other bags. The weight loss of bags increased proportionally relative to the lid hole sizes. Taken together, these results indicate that traditional cotton plugs are economically efficient, but 15 mm hole lids are the most efficient at maintaining hyphal growth and controlling water loss while allowing $CO_2$ emissions.

• 한국양식학회지
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• 제13권1호
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• pp.47-53
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• 2000

사육수조내 자연산란이 이루어지지 않는 범가자미 암컷을 대상으로 호르몬 처리에 의안 인위적 성숙.배란 유도 실험이 이루어졌다. 산란시기에 범가자미 체중 kg당 HCG 265~678 IU, 17${\alpha}$ 20${\beta}$ OHP 0.5~1.0${\mu}$g 농도의 복강주사와 LHRHa 63~81 ${\mu}$g의 펠렛을 투여한 결과, HCG농도 약 300 IU가 범가자미의 최정성숙과 배란유도에 효과적인 것으로 나타났다. 17${\alpga}$ 20${\beta}$ OHP와 대조군에서는 난의 성숙이 거의 진행되지 않았으며, LHRHa 펠렛 실험군에서도 난의 최종성숙과 배란이 일어나지 않았다. 따라서 범가자미 암컷의 경우 현재 많이 사용되고 있는 LHRHa 펠렛 투여보다도 저농도의 HCG (dir 300 IU/kg 체중)o 주사가 난의 최종성숙과 배란에 더 효과적인 것으로 나타났다.

• 한국균학회소식:학술대회논문집
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• 한국균학회 2018년도 춘계학술대회 및 임시총회
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• pp.29-29
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• 2018

Sparassis latifolia is called "Cauliflower Mushroom" and is known as an edible mushroom that has high content of ${\beta}$-glucan. Recently, artificial cultivation of S. latifolia has been done by bottle, plastic bag and wood cultivation in Korea. However it is not widely used because there are low incubation ratio and yield. For the high efficiency of production, we aim to find the superior strains and media for better mycelial and fruit body growth. First, we analyzed the genetic relationship among 31 strains and divided five groups with three kinds of URP primers. And then ten strains were selected from five groups based on the experiment of mycelial growth. The suitability of media for mycelial growth was different according to media type. The suitable solid and liquid media for mycelial growth of S. latifolia isolates were PDA and M2, respectively. In addition, with regard to C/N ratio, the mycelial growth increased even until C/N 160. Second, we investigated the production of fruitbody of the strains by plastic bag cultivation. The substrate was mixed with larch sawdust, corn flour, and wheat flour (8:1:1, v/v). Moisture content of substrate was controlled by about 60% with 10% molasses solution. Out of 31 strains, 19 strains formed primordia. The eight strains produced more than 140g/1kg in fresh weight. Third, molasses culture media was selected for the mycelial growth. And molasses suitable sugar content and input aeration were around 8Brix% and 0.3~0.6vvm, respectively. The longer the incubation period is, the more dried weight of mycelia increased, but medium volume decreased. Therefore, the best incubation period was 9 to 11 days depending on strains. In the future, research project entitled development of culture system and new variety for stable production of S. latifolia will be considered as a new item.

• 해양환경안전학회:학술대회논문집
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• 해양환경안전학회 2017년도 공동학술발표회
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• pp.82-82
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• 2017

우리나라로 회귀하는 연어는 북태평양에서 서식하는 연어 중 가장 넓은 영역에 분포하고 있으며, 경제적으로 매우 중요한 어류로서 이들의 자원관리를 위한 유전학적 연구가 활발히 진행되고 있다. 특히, 베링해와 북태평양 지역은 연어의 주 성장지로서 인접국가에서 산란된 연어들이 혼재되어 있어 국가별 연어의 계군을 구분할 수 있는 유전적 마커 개발 및 적용이 필요하고. 본 연구에서는 각 국가별 연어 집단에 대한 유전적 다양성, 차이점, 구조 및 인구통계학적 연구를 통한 유전학적 특징을 밝히고, 베링해와 북태평양 지역에서 성장하고 있는 연어의 국가별 계군의 분포와 이동 양상을 제시하려 한다.

• 한국버섯학회지
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• 제3권4호
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• pp.159-171
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• 2005

The supply and demand of mushrooms has increased dramatically as the standard of living has increased and the nutritional and medical value of the mushrooms has been discovered. However, in meeting the demand, the profitability of the mushroom is being reduced. Even though there are many different types of and uses for mushrooms, the overall output production for the last ten years has consistently increased 5.9% per year, and for the last five years('00~'04) 5.2 % per year, and for the last 3 years('02~'04) 2.9% per year- the rate of increase has slowed. Looking at the standard gross income from mushroom production, it has increased 5.2% over the last nine years('95~'03), 1.6% over the last 4 years('00~'03), and 3.2% over the last 3 years('02~'04)-the annual gross income is decreasing. Even though the farmers are producing more mushrooms, the annual gross income is not increasing enough to keep up with the production, and therefore the profitability of mushrooms is decreasing. Since 2002, the constant increase of the output of edible mushrooms such as the Common Mushroom, Oyster Mushroom, King Oyster Mushroom and Winter Mushroom has resulted in a price declines. Before 2004, Korea mainly exported mushrooms, but after 2004 Korea mainly imported mushrooms. The mushroom industry of Korea is nearing the end of the stage of 'supplying domestic demand.' The stages are as follows: demand raised ${\rightarrow}$ supply domestic demand ${\rightarrow}$ export ${\rightarrow}$ country with developed mushroom industry. Therefore, we should have an efficient system to promote the exportation of mushrooms. Not only the exportation of mushroom itself but also the exportation of mushroom spawn, cultivation techniques and processing facilities should be our goal when we formulate the policy.