• International Journal of Oral Biology
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• v.36 no.2
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• pp.59-64
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• 2011

Anthocyanins are naturally occuring phytochemicals and the main components of the coloring of plants, flowers and fruits. They are known to elicit antioxidative, anti-inflammatory and cancer preventive activity. In this study, we investigated anthocyanins in black / yellow soybean seedcoats using different methods of detection - thin layer chromatography (TLC), capillary zone electrophoresis (CZE) and HPLC analysis. The anthocyanins in soybean seedcoats were extracted by five independent methods of extraction and the aglycons (anthocyanidins) of the corresponding anthocyanins were prepared by acid mediated hydrolysis. The anthocyanin / anthocyanidin in black soybean seedcoat showed characteristic TLC mobility, CZE electrophoretic retention and HPLC migration time while little of anthocyanins were detected from yellow soybean seedcoat. The extracted anthocyanins showed pH dependent retention time in CZE and spectral change in UV-Vis spectrum. HPLC analysis of the hydrolyzed extract of black soybean seedcoat identified the presence of four anthocyanidins. The major anthocyanin in black soybean seedcoat was cyanin (cyanidin-3-O-glucoside), with the relative order of anthocyanidin in cyanidin > delphinidin > petunidin > pelargonidin.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.29 no.3
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• pp.280-284
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• 1984

Association of SMV infection in soybean plants with seedcoat mottling and with seed transmission of SMV was examined. Seedcoat mottling was increased positively in proportion to the SMV severity in variety Clark. No correlation was found between the incidence of mottled seed and the seed's position on the plant. Susceptible variety with virus symptoms gave rise to mottled seeds but only a small percentage of these mottled seeds could transmit the virus. SMV could be transmitted even by nonmottled seeds harvested from SMV infected plants in similar rate with mottled seed. It seemed that the amount of mottled seed could be used as an indicator of the amounts of SMV infection in a seed production field.

• Korean journal of applied entomology
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• v.21 no.1 s.50
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• pp.23-26
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• 1982

The present study was carried out to investigate the change of peroxidase activity of soybean seed infected with Cerrospora kikurhii. The protein content, polyphenol oxidase activity and peroxidase isozyme pattern in health and infected soybean seed were also compared. 1. The peroxidase activity was substantially higher in the infected soybean seeds than that in the healthy seeds either cracked or not. No significant differences in protein content were recognized among the seeds tested. 2. No significant differences in peroxidase activities and protein contents were notified between healthy and infected seeds from the measurements on each parts of dissected seeds, cotyledon and seedcoat, however the peroxidase activity in the seed coat of the stained seed was 2.5 times to healthy seed. 3. The activities of polyphenel oxidase were undectable in both healthy and diseased seeds. 4. The electrophoretic Patterns of the Peroridase isozyme were the same between healthy and in footed seed. 5. Therefore, the increase of peroxidase activity in infected soybean seedcoat was mainly due to the biochemical reaction against the pathogen.

• Korean Journal of Environmental Agriculture
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• v.18 no.1
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• pp.66-69
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• 1999

To investigate the hormetic effect of the low dose ${\gamma}-ray$, soybean(Glycine max L.) seeds of three cultivars (Hwangkeumkong, Bansakong, S900 cv.) were irradiated with the dose of 0.5∼20.0 Gy and cultivated in the experimental field. The hormetic effects of the low dose ${\gamma}-ray$ on the growth of field experiment were different from each cultivar, that is, more effective in the S900 cultivar of black seedcoat color than in the Hwangkeumkong and Bangsakong of yellow ones. The germination rate of S900 cultivar increased about 30% in the 0.5Gy irradiated group compared with that of the control. The plant height of the 4.0Gy irradiated group in both Bangsakong and S900 cultivar increased 15% more than that of the control. Grain yield and the number of pod in 1.0 Gy and 4.0 Gy irradiated group of S900 cultivar and that of 2.0 Gy irradiated group of Hwangkeumkong increased 25 and 35% on the average, respectively.