• 한국근적외분광분석학회:학술대회논문집
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• 한국근적외분광분석학회 2001년도 NIR-2001
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• pp.1021-1021
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• 2001

Sample presentation, which means how to set samples to an NIR instrument, is very important in Near Infrared (NIR) Spectroscopy. When sample presentation is not suitable for the samples that you use, very good spectra can not be obtained even if you use a sophisticated NIR instrument. In my presentation, various NIR sample presentations for agricultural products such as intact fruits, single grains, vegetable juice and the others will be explained. In case of peaches with thin peel, the fiber optics of Interactance can be used. However, the fiber optics are not suitable for oranges with relatively thick peel. In this case, transmittance method is useful. As for a small sample such as single grains, a specially designed cell is needed. The cell in transmittance mode has been developed and then applied to single kernels of rice and soybean. In this case we also used the fiber optics. As regards liquid type of sample, a cuvette cell made of quartz in transmittance mode is popular. However, it is time-consuming to wash and dry it. In order to compensate this disadvantage the sample presentation using normal test tubes as sample cells have been developed and applied to milk, rumen juice and urine of a milking cow. An individual test tube can be used for each sample if you use the calibration equation with sample cell compensation. The test tube cell has also been applied to spinach juice for determination of undesirable constituents. It is concluded that sample presentation is most important for NIR Spectroscopy.

• 한국식품조리과학회지
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• 제9권2호
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• pp.99-104
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• 1993

This study was carried out in order to develop supplementary foods for infants using Korean foods. Thirty-four different kinds of supplementary foods were developed and fourteen representative ones were selected to be analyzed chemically. The results are as follows: 1. The developed supplementary foods were 34 kinds and divided into 3 stages. First stage is designed for the babies just beginning to eat pureed vegetables and fruits. Second stage combined the nutritional attributes of both vegetables and meat. Third stage featured tender, bite-size pieces of meats and vegetables that appealed to the most mature tastes of basies. 2. In the production of prepared foods; water, milk, vegetable juice, fruit juice, and soy milk were used as the liquid source; rice, rice starch, chestnut, noodle, potatoes, sweet potatoes, rice cakes as the carbohydrate source; fish, meats soybean curd, beans, eggs, chicken, cow liver as the protein source; and vegetables and fruits were used as vitamin & mineral source. 3. The approximate composition range of the products were 10.91∼24.46% carbohydrate, 0.15∼6.06% protein, 0.092∼7% fat, 0.13∼ 1.37% ash, 0.63∼36.34% calcium, 0.092∼0.48% iron and 0.42∼16.36% vitamin C.

• Asian-Australasian Journal of Animal Sciences
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• 제23권10호
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• pp.1325-1332
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• 2010

This study was conducted to evaluate the effects of the particle size (PS) of alfalfa hay (AH) and soybean hull (SH) on milk production of dairy cows and the population of major cellulolytic bacteria in the rumen. Eight lactating Holstein cows, averaging $590{\pm}33\;kg$ BW and $47{\pm}13$ days in milk (DIM), were assigned in a $4{\times}4$ Latin square design to a $2{\times}2$ factorial arrangement of treatments: alfalfa hay particle size (fine vs. coarse) combined with soy hull (zero or substituted as 50% of AH). The cows were fed diets formulated according to NRC (2001). Physically effective factor (pef) and physically effective fiber (peNDF) contents of diets increased by increasing AH particle size and inclusion of SH in the diets (p<0.01). Dry matter intake was not significantly affected by treatments but intake of peNDF was increased marginally by increasing the PS of AH (p = 0.08) and by SH inclusion (p<0.01) in the diets. Milk production was increased by feeding diets containing SH (p = 0.04), but it was not affected by the dietary PS. Milk fat content was increased by increasing AH particle size (p = 0.03) and decreased by SH substitution for a portion of AH (p<0.01). The numbers of total bacteria and cellulolytic species were not affected by PS of AH or by SH. F. succinogenes was the most abundant species in the rumen followed by R. albus and R. flavefaciens (p<0.01). This study showed that SH cannot replace the physically effective fiber in AH having either coarse or fine particle size. In diets containing SH, increasing of diet PS using coarse AH can maintain milk fat content similar to diets without SH. Particle size and peNDF content of diets did not affect the number of total or fibrolytic bacteria in the rumen.

• Applied Biological Chemistry
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• 제28권2호
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• pp.56-61
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• 1985

전기전도도법과 화학적 분석방법을 병행하여 두부의 제조공정중 Ca염의 수지를 분석하였다. 전기전도도법에 의한 Ca염의 수지분석(收支分析)이 $CaSO_4$를 응고제로 사용한 경우에는 불가능하였으나, $CaCl_2$를 응고제로 사용한 경우에는 화학적 방법에 의해 구한 것과 잘 일치하였다. 두유 및 두부의 제조공정중 대두에서 두유로 이동되는 고형분량과 Ca량은 각각 66%, 63%였고, 대두에서 두부로 이행되는 고형분은 47.8%였다. $CaCl_2$를 응고제로 사용한 경우 두부중 Ca 농도$(Y_{Tofu},\;mg{\cdot}Ca/g{\cdot}Tofu,\;wet\;basis)$ 및 순물중 Ca농도$(Y_{drained\;soln},\;mg{\cdot}Ca/ml{\cdot}drained\;soln)$는 두유중 첨가되는 $CaCl_2$의 량$(C,\;mg{\cdot}CaCl_2/ml{\cdot}soy\;milk)$에 비례해서 증가하였고, $81{\pm}0.5%$의 수분을 포함한 두부에 대하여 r=0.9898에서의 관계를 $Y_{Tofu}=0.3369C+1.2689$ 얻었고, 순물에 대해서는 r=0.9991에서 $Y_{drained\;soln}= 0.2899C+0.0399$의 관계를 보였다. $CaCl_2$를 응고제로 사용할 경우 원료대두를 제외한 모든 공정에서 전기전도도법에 의해 Ca 농도를 측정할 수 있음이 밝혀졌다.

• 한국토양비료학회지
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• 제19권2호
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• pp.157-165
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• 1986

전보(前報)에 이어 우리나라 전국(全國) 60개(個) 밭 토양(土壤)에서 분리(分離)한 토착근류균(土着根瘤菌)을 대두작부형별(大豆作付型別)로 구분(區分)하여 질소고정력(窒素固定力) 및 몇가지 생리적(生理的) 특성(特性)을 비교(比較) 검토(檢討)한 결과(結果)는 다음과 같다. 1. 토착근류균(土着根瘤菌)을 대두재배지(大豆栽培地)로 부터 133개균주(個菌株), 무재배지(無栽培地)에서 125개균주(個菌株)를 분리(分離)하여 free-living 상태(狀態)에서 아세칠렌 환원력(還元力)을 측정(測定)한 결과(結果), 전자(前者)에서 12.8%, 후자(後者)에서는 6.4%로 평균(平均) 9.7%가 4n mole $C_2H_4$/tube/hr 이상(以上)의 아세칠렌 환원력(還元力)을 보였다. 2. 토착근류균(土着根瘤菌)의 분리(分離) 326개(個) 균주(菌株)를 litmus milk 검정(檢定)으로 산(酸) 및 알카리 생성반응(生成反應)을 조사(調査)한 결과(結果), 산(酸) 생성균(生成菌)은 대두재배지(大豆栽培地)에서 분리(分離)한 균(菌)은 34%, 무재배지(無栽培地)의 분리균(分離菌)은 20%였으나, 알카리 생성균(生成菌)이 산(酸) 생성균(生成菌) 보다 두 토양(土壤) 모두 많았다. 또한 토착근류균(土着根瘤菌)중 serum zone 양성균주(陽性菌株)는 대두무재배지(大豆無栽培地)의 분리균(分離菌)에서 68%, 대두재배지(大豆栽培地)의 분리균(分離菌)이 47%로서 대두무재배지(大豆無栽培地)의 분리균(分離菌)에서 많은 serum zone 양성반응(陽性反應)을 보였다. 3. 토양심층별(土壤深層別) 토착근류균수(土着根瘤菌數)는 하층토 보다 표토층에서 현저(顯著)히 많았으나, 작부형간(作付型間)에는 큰 차이(差異)가 없었다. 그러나 이들 균수(菌數)중 대두(大豆)에 감염(感染)할 수 있는 토착대두근류균(土着大豆根瘤菌)은 대두재배지(大豆栽培地)에서 2.3~23.0 cells/g soil, 대두무재배지(大豆無栽培地)에서 0.9~9.2 cells/g soil로서 표토층에서 하층토로 갈수록 현저(顯著)히 감소(減小)하였다.

• 한국초지조사료학회지
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• 제19권1호
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• pp.63-74
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• 1999

본 시험은 수단그라스와 청예 대두와의 간작과, 단작재배시 생육단계별 채식율 및 자유채식량을 검토하기 위하여 실시 하였다. 그 결과를 요약하면 다음과 같다. 수단그라스와 대두 간작재배는 단작에 비하여 단백질 함량을 증가시키는 경향이 뚜렷하게 나타났으나 ADF 함량에 있어서는 차이를 나타내지 않았다. 채식율(엽, 경, 수)은 통일 생육단계에서 간작재배가 단작에 비하여 높게 나타났으나, 유기숙 이후부터는 간작 단작 모두 떨어지는 경향을 보였다. 또한 생초의 자유채식량에 있어서도 같은 경향을 보였으며, 특히 간작의 180cm구는 $516.7g/kg^{0.75}$으로 가장 높은 채식량을 보였던 반면, 황숙기구는 $197.6g/kg^{0.75}$로 가장 적은 수치를 보였다. 건물섭취량은 간작구가 단작에 비하여 통일 생육 단계시 채식량이 높은 경향을 보였으며, 특히 간작의 유숙기구는 $98.4g/kg^{0.75}$으로서 가장 높은 채식량을 나타냈다. 간작구의 단백질 섭취량은 180cm, 250cm, 수잉기, 출수기, 유숙기에 각각 9.6, 8.5, 7.2, 7.2, $7.2g/kg^{0.75}$으로 높은 수치를 보였지만, 단작은 5.5, 4.2, 4.0, 4.8, $3.7g/kg^{0.75}$으로 간작에 비하여 매우 떨어지는 경향을 보였다. 이상의 결과를 종합해 볼 때 수단그라스 교잡종과 대두와의 간작은 단작재배에 비하여 채식율, 채식량, 단백질 섭취량을 증가시켜주는 재배 방식으로 사료된다.

• Current Research on Agriculture and Life Sciences
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• 제7권
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• pp.23-32
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• 1989

대두(大豆)를 이용(利用)한 요구르트 및 유산균(乳酸菌) 음료(飮料)의 제조(製造)를 위(爲)하여 두유(豆乳) 요구르트에 대한 유산균(乳酸菌) 생육(生育)과 산생성(酸生成) 및 여러가지 첨가물(添加物)에 대한 영향(影響)을 조사(調査)하였다. 시비조건(施肥條件)에 차이(差異)를 두어 재배(栽培)한 대두(大豆)를 이용(利用)한 두유(豆乳)에서의 유산균(乳酸菌) 생육(生育) 및 산생성(酸生成)은 큰 차이(差異)가 없었으나 개발복비(開發複肥)를 사용(使用)한 Soybean D가 다소(多少) 양호(良好)한 것으로 나타났으며, 두유(豆乳), 탈지두유 및 분리대두단백(分離大豆蛋白)에서의 산생성(酸生成)은 두유(豆乳)가 탈지두유보다 양호(良好)했으며 분리(分離) 대두단백(大豆蛋白)으로 조제(調製)한 두유(豆乳)에서는 현저(顯著)히 낮았다. 두유(豆乳) 요구르트의 제조(製造)에 사용(使用)한 균주별(菌株別) 산생성능력(酸生成能力)은 L. bulagricus와 L. acidophilus가 우수(優秀)하였다. 당(糖)과 각종(各種) 발육촉진물질(發育促進物質)의 첨가(添加)에 의해 균증식(菌增殖) 및 산생성(酸生成)이 촉진(促進)되었으며 특(特)히 Glucose와 Yeast Extract의 첨가(添加)는 현저(顯著)한 효과(效果)를 나타내었다.

• Current Research on Agriculture and Life Sciences
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• 제5권
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• pp.40-51
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• 1987

광교(光敎)를 비롯한 12개품종(個品種)의 대두근류(大豆根瘤)로부터 탐색분리(探索分離)한 질소고정균(窒素固定菌)의 생리적(生理的) 특성(特性) 및 숙주작물(宿主作物)과의 접종친화성(接種親和性)을 조사(調査)한 결과(結果)는 다음과 같다. 1. YMA배지(培地)에서 자란 질소고정균(窒素固定菌)의 색상(色相)은 백색상(白色相), 반투명색상(半透明色相), 투명색상(透明色相)이 각각(各各) 60%, 30%, 10%였으며 Litmus milk 반응(反應)은 alkali, acid serum, alkali serum, acid가 각각(各各) 51%, 29%, 9%, 11%로 나타났다. 2. 우량질소고정균(優良窒素固定菌)의 YM배지(培地)에서의 생육속도(生育速度) 및 pH의 변화(變化)는 S 022, S 096이 생육속도(生育速度)가 느리고, alkali를 생성(生成)하였으며 S 080, S 090, S 118은생육속도(生育速度)가 빠르고 산(酸)을 생성(生成)하였다. 3. YM배지(培地)의 초기(初期) pH가 6.0~7.0의 범위(範圍)에서 Rhizobia의 생육(生育)이 가장 좋았다. 4. 질소원(窒素源)으로서 glutamine, asparagine, allantion 등(等)은 Rhizobia의 생육(生育)을 촉진(促進)시켰다. 5. 분리(分離)한 균주(菌株) 전부(全部)가 대두(大豆)에 근류(根瘤)를 형성(形成)시켰으며, 우량질소고정균주(優良窒素固定菌株)는 그중 색상(色相)이 백색(白色)이고 colony가 소형(小型)이며 질산환원력(窒酸還元力)이 있고 아질산환원력(亞窒酸還元力)이 없으나 비교적(比較的) 높은 질소고정력(窒素固定力)을 나타냈다. 6. 동일균주(同一菌株)라도 숙주작물(宿主作物)의 친화성(親和性)에 따라서 질소고정능력(窒素固定能力)에 차이(差異)가 있었으며, 질소고정능력(窒素固定能力)이 없는 무효근류(無效根瘤)를 형성(形成)하는 균주(菌株)도 있었다.

• Asian-Australasian Journal of Animal Sciences
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• 제18권10호
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• pp.1421-1424
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• 2005

The aim of this study was to evaluate the effect of two diets different in crude fibre content and ingredients on performance and on caecal characteristics of rabbits around weaning. Thirty litters from thirty New Zealand White does were divided at Day 18 in two groups fed, respectively, a low fibre concentrate (LFC, consisting mainly of soybean meal, delactated whey, barley) from Day 18-28 followed by a creep feed (CF, consisting mainly in alfalfa meal, barley and wheat bran) from Day 29-32, and a CF from Day 18-32. After weaning (32 days) both groups were fed the CF ad libitum for two weeks. During the pre-weaning period, mortality, milk intake and solid feed intake (from Day 20) were recorded daily, while the live weight of kits was recorded twice, at 18 and 32 days. At day 28, one rabbit/litter was slaughtered in order to obtain data on caecal content characteristics. After weaning, the rabbits were located in collective cages, feeding ad libitum CF; feed intake, live weight and mortality were recorded weekly for two weeks. During the preweaning period, there were no differences between the groups in milk and solid feed intake and, by consequence, in live weight at weaning; instead, the mortality was higher (12.5 vs 4.5%) for the group (A) that changed diet at 28 days. Group A showed also a higher caecal pH (6.12 vs. 5.72), propionate to butyrate ratio (0.73 vs. 0.46), ammonia content (9.3 vs. 7.1 mmol/l), but a lower total volatile fatty acid content (66.8 vs. 82.1 mmol/l) than B Group, probably due to the dried milk whey in the concentrate. After weaning, there were no significant differences between the two groups. The authors concluded that the use of a low fibre concentrate for suckling rabbits is not recommended.

• Nutrition Research and Practice
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• 제13권4호
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• pp.323-332
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• 2019

BACKGROUND/OBJECTIVES: The aim of this study was to analyze specific foods influencing absolute nutrient intake and between-person variations of nutrient intake among Korean preschoolers. SUBJECTS/METHODS: This study included 2,766 participants aged 1-5 years in the 2009-2013 Korea National Health and Nutrition Examination Surveys. Dietary data were obtained from a 24-h dietary recall method. Major food sources of absolute nutrient intake were evaluated based on percent contribution of each food. To assess the contribution of specific foods to between-person variations in nutrient intake, stepwise multiple regressions were performed and cumulative $R^2$ was used. RESULTS: White rice and milk were main food sources of energy, protein, carbohydrate, phosphorus, iron, potassium, thiamin, riboflavin, and niacin. The percentage of fat contributed by milk was 21.3% which was the highest, followed by pork, soybean oil, and egg. White rice accounted for 25% and 40% of total variability in total energy and carbohydrate intakes, respectively. About 39% of variation in calcium intake was explained by milk while 40% of variation in phosphorous intake was explained by cheese. The top 10 foods contributing to between-person variations in nutrient intakes were similar with food items that mainly contributed to absolute nutrient intakes. The number of foods explaining 90% of absolute amounts of nutrient intakes varied from 28 for vitamin A to 80 for iron. CONCLUSIONS: This study identified specific foods that contributed to absolute nutrient intakes and between-person variations in nutrient intakes among Korean preschoolers. Our findings can be used to develop dietary assessment tools and establish food-based dietary guidelines for young children.