• 생약학회지
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• 제27권3호
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• pp.190-195
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• 1996

Wildbean(Glycine soja Siebold et Zucc.) is known as the orgin of soybean (Glycine max Merr.). Based on the hypothesis that the secondary transformation of chemical compound in wildbean might have occurred during its breed improvement to soybean. this study was carried out to compare the antioxidant activities and chemical composition in wildbean and soybean. The present study demonstrates that 1) Antioxidant activity was much higher in EtOAc, extract of wildbean than in soybean. 2) strong antioxidant activity observed in EtOAc extract of wildbean was due to the presence of (-)-epicatechin, which was not present in the extract of soybean but isolated, for the first time, from the extract of wild bean, and 3) antioxidant activity of the isolated (-)-epicatechin was greater than that of tocopherol, the previously known antioxidant.

• 동의생리병리학회지
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• 제24권4호
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• pp.668-673
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• 2010

The purpose of this research was the comparative study of 3 kinds of black soybean on murine immune cells. The 3 kinds of black soybean are Glycine max Merr. with inner color-yellow (GY), Glycine max Merr. with inner color-greenish (GG) and Rhynchosia volubilis Lour. (RV). All of the black soybean increased the viability of murine thymocytes in vitro. The combined treatment of GY or GG and mitogen did not affect the viability of splenic T- and B-lymphocytes compared with mitogen-treated group, but the combined treatment of RV and mitogen increased their action compared with mitogen-treated group. Also, the 3 kinds of black soybean were given p.o. once a day for 7 days, respectively. RV increased the population of thymic-$CD8^+$, splenic-$CD8^+$ and $B220^+$ cells in vivo. Furthermore, GY and GG did not affect the phagocytic activity and the production of nitric oxide in peritoneal macrophages in vitro, but RV enhanced their action. These results suggest that immunopotentiative action of Rhynchosia volubilis Lour. is more potent than their of Glycine max Merr.

• Applied Biological Chemistry
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• 제30권2호
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• pp.153-162
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• 1987

한국에서 시험재배 되고 있는 대두(Glycine max.) 101품종의 근류로 부터 근류균을 분리하여 AMA 고체배지 상에서의 생육속도 및 근류형성력에 따라 fast-growing soybean rhizobia 7 균주와 slow-growing soybean rhizobia 9균주를 선발하였다. 선정균의 질소고정력, 미생물학적 특징 및 생화학적 특징을 조사한 결과 slow-growing soybean rhizobia 는 .Bradyrhizobium japonicum 과 일치하였으며, fast-growing soybean rhizobia는 Rhizobium fredii 와 유사하였다.

• 자연과학논문집
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• 제5권1호
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• pp.53-57
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• 1992

Aspergillus niger ATCC 16513과 대두(Glycine max. L)의 정제 $\alpha$-galactosidase를 사용 하여 몇가지 이들의 kinetic성질을 조사 하였다. Asp. niger $\alpha$-galactosidase의 raffinose와 stachyose에 대한 Km값은 각각 37.0mM과 55.5mM, 대두 $\alpha$-galactosidase는 50.0mM과 55.5mM로서 PNPG보다 이들에 대한 친화성이 적었다. 또한 galactose는 ASP. niger와 대두 $\alpha$-galactosidase 모두의 활성을 저해 하였으나 2-mereaptoethanol과 L-cystene은 대두 $\alpha$-galatosidase의 활성만을 약간 저해 하였다. Asp. niger와 대두 $\alpha$-galactosidase의 활성에 관여하는 아미노산은 diethyl pyrocarbonate에 의한 화학수식에 의하여 histidine임이 확인 되었고 Asp. niger $\alpha$-galactosidase의 1mole당 아미노산 잔기수는 모두 902개, 대두$\alpha$-galactosidase는 286개 이었다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.145-145
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• 2017

Foxglove aphid, Aulacorthum solani (Kaltenbach), is a Hemipteran insect that infected a wide variety of plants worldwide and caused serious yield losses in crops. The objective of this study was to identify the putative genes to foxglove aphid resistance in wild soybean, PI 366121 (Glycine soja Sieb. and Zucc.). One hundred and forty-one F4:8 recombinant inbred lines developed from a cross between susceptible variety, Williams 82 and foxglove aphid resistance wild soybean, PI 366121 were used. The two type of resistance response, antibiosis and antixenosis resistance were evaluated through choice and no-choice test, graded by the degree of total plant damage and primary infestation leaf damage; a genome-wide molecular linkage map was constructed with 29,898 single-nucleotide polymorphism markers utilizing a Axiom(R) 180K soyaSNP array. Using inclusive composite interval mapping analysis for foxglove aphid resistance, one major candidate QTL on chromosome 7 was identified. The major QTL on chromosome 7 showed both antixenosis and antibiosis resistance responses. The newly identified major QTL was consistent with previously reported QTL, Raso2, which showed around 5 times narrow down interval range with 8 candidate genes. Furthermore, total 1,115 soybean varieties including Glycine soja and Glycine max were exposed to germplasm screening, and 31 varieties, which showed significant antibiosis type foxglove aphid resistance were identified. This result could be useful in breeding for new foxglove aphid resistant soybean cultivars and developing novel insecticides.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.106-106
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• 2017

The soybean [Glycine max (L.) Merr.] is one of the most important crop resources worldwide as food and forage. It is also important and valuable that to hold crop resources to have high genetic diversities. Recently, a core collection has been constructed in many plants to preserve the genetic resources of various plants. A core collection is small population to represent the genetic diversity of the total collection, and is of strategic importance as they allow the use of a small part of a germplasm collection that is representative of the total collection. Here, we developed the core collection consisting of 816 accessions by using approximately 180,000 (180K) single nucleotide polymorphisms (SNPs) developed in previous study. In addition, we performed genetic diversity and population structure analysis to construct the core collection from entire 4,392 collections. there were excluded sample call rates less than 93% and duplicated samples more than 99.9% according to genotype analysis using 180K SNPs from entire collections. Furthermore, we were also excluded natural hybrid resources which Glycine max and Glycine soja are mixed in half through population structure analysis. As a result, we are constructed the core collection of genetic diversity that reflects 99% of the entire collections, including 430 cultivated soybeans (Glycine max) and 386 wild soybeans (Glycine soja). The core collection developed in this study should be to provide useful materials for both soybean breeding programs and genome-wide association studies.

• Advances in Traditional Medicine
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• 제4권4호
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• pp.227-234
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• 2004

An ${\alpha}-Amylase$ was purified to apparent homogeneity from germinating soybean seeds (Glycine max). Enzyme showed high specificity for starch. ${\alpha}-Amylase$ from soybean has optimum pH at 7.6 in the pH range 4.0-10.6. At this pH, the $K_m$ of starch was 2.63 mg/ml and the $V_{max}$ was equal to 52.6 mg/ml/min protein. Optimum temperature of the enzyme was found to be $55^{\circ}C,\;Q_{10}$ equal to 1.85 and energy of activation equal to 12 kcal/mol. Additives like, EDTA reduced the activity of ${\alpha}-amylase$ whereas PMSF enhanced the activity. ${\alpha}-Amylase$ was inhibited by several heavy metal ions.

• 생약학회지
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• 제25권4호통권99호
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• pp.342-347
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• 1994

Soybean and beanpaste have been used as ore of the important food in Korea. Beanpaste is made from soybean(seed of Glycine max $M_{ERRIL}$, Leguminosae) by traditional fermentation. It was expected that the secondary metabolites might be formed by the action of some microorganisms or enzymes on the constituents primarily contained in soybean during manufacturing or storage of beanpaste. A systematic investigation on the chemical constituents was carried out. As a result, sterol glycoside named daucosterin, lipo-daucosterin have been isolated from the lipid fraction soybean and their structures elucidated basis of chemical and physicochemical evidences. A ratio of composition of the lipid constituents in soybean and beanpaste were estimated.

• 한국미생물·생명공학회지
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• 제51권3호
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• pp.306-308
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• 2023

This research presents the whole-genome sequence of Enterobacter asburiae strain IK3, which was isolated from the rhizosphere soil of soybean (Glycine max). The genome of the strain is composed of a single chromosome with 4 plasmids, total size of 5,084,040 bp, and the GC content is 55.5%.

• 생태와환경
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• 제54권2호
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• pp.120-124
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• 2021

In order to understand the vegetative role of Glycine soja, we studied the basic physiological characteristics between Glycine soja and Glycine max. For this study, the light intensity (μmol m^-2 s^-1) on leaf surface, leaf temperature (℃), transpiration rate (mmol m^-2 s^-1), photosynthetic rate (μmol m^-2 s^-1), substomatal CO₂ partial pressure (vpm) of Glycine soja and Glycine max were measured, and the quantum yield, photosynthesis rate per substomatal CO₂ partial pressure were calculated. In the results of simple regression analysis, the increasing quantum yield decreases leaf temperature both of Glycine soja and Glycine max and the increasing leaf temperature decreases transpiration rate in case of Glycine soja. However, in case of Glycine max, the increasing leaf temperature decreases substomatal CO₂ partial pressure, photosynthetic rate, and photosynthetic rate per substomatal CO₂ partial pressure as well as transpiration rate. Also, increasing transpiration rate increases substomatal CO₂ partial pressure while decreases photosynthetic rate per substomatal CO₂ partial pressure. Thus, Glycine soja is relatively more easily adaptable to severe environments with low soil nutrients and high light levels. Compared to Glycine max susceptible to water loss due to a water-poor terrestrial habitat, the physiological traits of Glycine soja has a high average transpiration rate and are less susceptible to water loss will act as a factor that limits the habitat according to soil moisture.