• Proceedings of the Korean Society of Organic Agriculture Conference
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• 2009.12a
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• pp.296-296
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• 2009

국제기준(Codex)에 부합하는 유기농산물의 생산을 위해서는 저항성 품종의 유기종자 사용을 원칙으로 규정하고 있으나, 현재까지 우리나라의 경우는 찰옥수수 등 주요 식량작물에 대한 유기채종기술이 거의 전무한 실정이다. 본 연구는 찰옥수수 유기채종기술 확립을 위한 기초 자료로 이용하고자 찰옥수수 유기 채종에 따른 파종기별 생육 및 수량특성에 대하여 검토하였다. 유기종자 생산을 위한 채종시험은 홍천군 남면에 위치한 농가 유기포장에서 수행되었으며, 시험품종은 옥수수시험장에서 육성 보급되고 있는 미백2호 및 미흑찰 원종종자를 이용하였다. 파종기는 4월 16일, 5월 4일, 5월 27일 등 3처리로 구분하여 모 부본 동시파종 하였다. 재식밀도는 80${\times}$25cm로 하여 점파하였고 모 부본의 재식비율은 2:1로 웅주간파 하였다. 잡초방제는 흑색 P.E. Film으로 멀칭 재배하였고, 유기질비료는 H사에서 생산된 혼합유박비료를 전량 기비로 시용하였다. 파종기별 출사일수는 미백2호의 경우 4월 파종에서 79일로 가장 길었고, 파종 시기가 늦을수록 출사일수는 짧아지는 경향을 보였다. 미흑찰의 경우에는 미백2호의 결과와 같은 경향으로 파종시기가 늦을수록 출사일수가 빨라지는 경향을 보였으며, 간장 및 착수고 또한 미백2호와 미흑찰 모두 4월 파종에서 가장 높았고, 파종기가 늦을수록 낮아지는 경향이었다. 이삭특성에 있어서 이삭장과 착립장은 미백2호 및 미흑찰 모두 4월 중순 및 5월 상순 파종간에 큰 차이가 없었으나, 5월 하순 파종에서 현저하게 짧아지는 경향을 보였다. 발아율의 경우 미백2호는 4월 중순 및 5월 상순 파종에서, 미흑찰은 5월 상순 파종에서 높게 나타났다. 종실중과 100립중은 미백2호 및 미흑찰 모두 4월 중순과 5월 상순 파종간에 큰 차이가 없었고, 5월 하순 파종에서 현저하게 감소하였다. 10a당 채종수량은 미백2호의 경우 4월 상순 파종에서 217㎏으로 가장 높았고, 미흑찰은 5월 상순 파종에서 164kg/10a으로 가장 많았으며, 두 품종 모두 5월 하순 파종에서 채종수량의 뚜렷한 감소가 나타났다. 따라서 찰옥수수 유기종자의 안정적 채종과 정상수량 확보를 위해서 미백2호는 4월 중순 파종시에 그리고 미흑찰은 5월 상순 파종시에 채종 안전성이 가장 우수한 것으로 판단되었고, 또한 채종수량에 직접적으로 영향을 미치는 1개 이삭당 종실중과 100립중도 크게 증가하는 것으로 나타났다.

• Korean Journal of Medicinal Crop Science
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• v.5 no.1
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• pp.67-71
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• 1997

Bupleurum falcatum is one of the oldest medicinal crop spontaneously generated as well as cultivated by man and successfully grown during the summer season in Korea. This study was conducted to examine the effect of planting density in the row spacing and hill spacing system using machine seeder on major agronomic traits, yield and labor saving. In 1993 and 1994, a spilt plot design was used with row spacing as the main plot and hill spacing as subplot. Labor saving efficency of the machine seeder reduced 96% than the hand seeding in sowing time required. Number of seeding stand increased, but stem height and number of nodes did not affect with different density in each row and hill spacing. There were reduced with narrow hill spacing on stem diameter, number of branches, shoot weight and root traits. There was no significant interaction between row and hill spacing for any major traits studied except shoot weight. Yield was more increase at 20cm than at 30cm row spacing. For these studies the optiomum density appears to be between drilling or 5cm hill spacing with 20cm row spacing. A highly significant positive correlation was appeared between number of seedling stand and root yield.

• Korean Journal of Weed Science
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• v.30 no.2
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• pp.84-93
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• 2010

Petri dish and pot experiments were conducted to investigate germination and seedling emergence of Ammannia coccinea as influenced by environmental factors. The best germination of A. coccinea was obtained at $35/30^{\circ}C$ of temperature and 0 bar of osmotic potential, while no germination at temperatures of ${\leq}$ $15^{\circ}C$ and ${\geq}$ $40^{\circ}C$, osmotic potentials of ${\leq}$ -2.0 bar, or dark condition. The best seedling emergence was observed at $35/30^{\circ}C$, at which the first emergence of A. coccinea was observed at 7 days after sowing (DAS) with its maximum emergence reached at 10 DAS. No seedling emergence was observed at $15/10^{\circ}C$ with significant reduction at $40/35^{\circ}C$. Seedling emergence decreased with increasing soil depth, resulting in no seedling emergence at ${\geq}$ 3 cm. The Gompertz model well described the cumulative germination and seedling emergence of A. coccinea with time. Germination influenced by osmotic potential and seedling emergence influenced by soil burial depth were well described by the logistic model. Overall results indicate that A. coccinea is photoblastic and requires temperatures greater than $15^{\circ}C$, osmotic potential greater than -2.0 bar, and soil burial depth shallower than 3 cm for its germination and seedling emergence, which were faster than M. vaginalis but slower than E. crus-galli.

• Korean journal of applied entomology
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• v.36 no.2
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• pp.145-149
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• 1997

Occurrence pattern of major insect pests on 7 recommended potato cultivars in Korea was investigated from sowing to harvesting time at Kangneung and Daegwallyung experiment fields in 1996. Green peach aphid (Mym persicae Sulzer), potato aphid (Macrosiphum euphorbiae Thomas) and beet armyworm (Spodoptera enigua Hubner) were the major insect pests on potato leaves, and wireworm (Selatosomus puncticollis Mot.) was the major pest on tubers. There was a slight difference of average number of aphids per 50 leaves among cultivars; ranged from 22.7 on cv. Dejima to 46.3 on cv. Superior. Numbers of leaves damaged by beet armyworm larvae on cv. Shepody and cv. Jopung were 11.0 and 14.3, and these cultivars are thought to be resistant against the larvae infestation. However, degree of damage on cv. Dejima and cv. Namsuh was higher 10 times than cv. Shepody and cv. Jopung. In wireworm, the percentage of damaged tubers on cv. Irish Cobbler was lowest of 8.1%, and followed by cv. Superior, cv. Dejima, cv. Jopung, and cv. Atlantic. Whereas, those on cv. Namsuh and cv. Shepody were significantly high of 50.0% and 46.8%.

• Weed & Turfgrass Science
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• v.3 no.3
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• pp.232-239
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• 2014

Zoysiagrass seeds had low germination or lack of germination uniformity under natural condition. To improve the final germination percentage of zoysiagrass seeds, we investigated the methods of proper pre-treatment to enhance germination rate of zoysiagrass. Seeds were subjected to the testa scarification and four types of seed priming methods, namely, hydro priming (distilled water), osmotic priming (polyethylene glycol -0.5 MPa, -1.0MPa, -2.0 MPa), halo priming (Potassium nitrate 100 mM, 200 mM, 400 mM), and solid matrix priming (seed : microcel-E : distilled water = 4:1:8) with and without 25% KOH treatment in the testa. The scarification improved the germination rate and reduced the germination time. Osmotic priming (PEG -0.5 MPa) with scarification were significantly increased the seed germination rate and decreased MGT, and $T_{50}$. Seed germination rate obtained by osmotic priming (PEG -0.5MPa) with scarification increased above 60% in comparison with natural condition. Conclusively, scarified and primed zoysiagrass seed germinated more rapidly and uniformly in the study. The present results suggested that PEG priming treatment with KOH treatment prior to sowing could improve germination percentage, and this method makes us establish the early zoysiagrasses in the field.

• Journal of Bio-Environment Control
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• v.12 no.1
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• pp.7-11
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• 2003

Cellular glass foam (CGF), the reprocessed glass, has a possibility to be used as a medium component in plug culture of horticultural crops due to the its excellent air and water permeability as comparable to perlite. An experiment was conducted to investigate growth of plug seedlings of Lycopersicum esculentum 'Segye' as influenced by irrigation frequency in various medium combinations of CGF (2.0-4.0 mm particle size) and peatmoss. Seeds were sown in 200-cell plug trays, filled with mixtures of CGF and peatmoss either at 33：67 or 25：75 (％. v/v) and were germinated on a fogged propagation bed. The irrigation frequencies used were one, two or three times per every two days. A commercial plug medium (Tosilee medium) was used as the control, and the irrigation frequency in the control was one time per day. Growth of seedlings, and medium pH and EC were measured at 33 days after sowing. The medium composition had little influence on overall growth of seedlings. Irrigation frequency very significant affected number of leaves, leaf area, chlorophyll concentration, fresh and dry weights of shoots and roots, and dry matter. Growth of seedlings was the greatest with the highest irrigation frequency in the 25% CGF＋75% peatmoss mixture.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.04a
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• pp.53-53
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• 2019

Cypripediums, popularly called lady's slippers or moccasin flowers, are the showiest and most sought after hardy terrestrial orchids, collected and grown by orchid and alpine plant enthusiasts alike. In Korea, 4 species of cypripedium are reported as Cypripedium japonicum, C. macranthos, C. guttatum, and C. calceolus. We had already reported the feasibilities of C. macranthos and C. guttatum with in vitro germination methods from immature seeds. The seeds of white lady's slipper orchid (Cypripedium macranthos Sw. alba) were collected 65 days after pollination in 2018. The green pods were sterilized with flame and sowed immediately on the POM(Phytomax orchid maintenance media(R), Sigma) supplemented with BAP 0, 0.5, 1.0 mg/L and NAA 0, 1, 2mg/L. The germination of seed was observed 90 days after sowing, and the plantlets were subcultured to the same media according to the size of the protocorm with 1~2, 2~3, 3~4, 5~6, 7~8mm. The time of the subculture to the new media seems to be critical factors of forming rhizoids which is the hairy root of the cypripediums. As a results, the protorms of the white lady's slipper orchid was successfully germinated in the POM media supplemented BAP 0.5 and NAA 1.0 mg/L. The roots and rhizoids were formed in 5~6mm protocorms subculture over 95% survival ratio. We also tried to subculture to liquid medium without activated charcoal, however the browning or malformation of the roots was observed in the root. The formation of shoots from the protocorm was effectively enhanced in the POM media with non-additives of plant growth regulators. These results indicate the possibility of high and stable production and practical industrialization of endangered white lady's slipper orchids.

• Korean Journal of Medicinal Crop Science
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• v.27 no.2
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• pp.126-135
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• 2019

Background: The ginseng ginsenosides, which have various physiological activities, are known to be more abundant in the leaves than in the roots, and the consumers' interest in ginseng sprout as a functional vegetable has been increasing. Methods and Results: The aim of this study was to investigate the effects of growth period on growth properties, active ingredients and rheology of ginseng sprouts cultivated in a non-heated greenhouse equipped with a shade net for 60 days, starting from the end of May to the middle of July. The chlorophyll content of the leaves decreased, but their length and width increased with increasing cultivation days. In particular, growth increased significantly until 40 days, but only slightly after 50 days. The stem length did not increase greatly from the 20 th to the 30 th day of cultivation, but increased significantly from the 30 th to the 40 th day, and then further increased gradually. The weight of the leaves, stems, and roots increased slightly, but not change significantly. After 40 days of cultivation, the total ginsenoside content increased by 1.07 times in the leaves and decreased by 0.80 times in the roots with increasing cultivation days. The leaf contents of ginsenosides $Rg_1$, Re, $Rb_1$, Rc, $F_3$ and $F_4$ increased with increasing cultivation days. The rheological properties of ginseng sprout showed the greatest influence on stem hardening with increasing cultivation days. Conclusions: Therefore, based on the growth characteristics, active ingredients and physical properties, 40 days after sowing was considered to be an appropriate harvesting time for ginseng sprouts.

• Journal of Bio-Environment Control
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• v.30 no.3
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• pp.218-229
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• 2021

Recently, as consumers' interest and importance in health care have significantly increased, they prefer natural and organic foods that do not use chemical pesticides. Since sprout vegetables effectively promote health and prevent diseases such as cancer and cardiovascular disease, the consumption of sprout vegetables, a highly functional and safe food, has been increased significantly. This study aimed to investigate the effect of light on the growth and bioactive compounds of seven different sprout vegetables. After sowing the seeds of various sprout vegetables (kale, Chinese kale, broccoli, red cabbage, alfalfa, red radish, and radish), the sprouts were cultivated under light conditions (20℃, RGB 6:1:3, 130 μmol·m^-2·s^-1, 12 hours photoperiod) and dark condition for 7 days. Sprouts samples were taken at 1-day intervals from 4 to 7 days after treatment. The fresh weight, dry weight, plant height, total phenol content, and antioxidant capacity were measured. Brassica species (kale, Chinese kale, broccoli, red cabbage) and Medicago species (alfalfa) had significantly higher fresh weight values under dark conditions, while the content of bioactive compounds was increased considerably under light conditions. In contrast, the fresh weight of Raphanus genus (red radish, radish) significantly increased under the light condition, but the antioxidant phenolic compounds were significantly higher under the dark state. A negative correlation was observed between the growth and secondary metabolites in various sprout vegetables. This study confirmed the effect of light and dark conditions on different sprout vegetables' growth and nutritional value and emphasizes the importance of harvest time in producing high-quality sprout vegetables.

• Journal of Bio-Environment Control
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• v.32 no.3
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• pp.217-225
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• 2023

This experiment was conducted to investigate the effects of microwave irradiation on the growth and secondary metabolite contents of lettuce seedlings. Seedlings at three weeks after sowing were treated by a microwave oven for 0, 4, 8, and 12 seconds with 200 W. After cultivation in a close plant production system for 4 weeks, plant growth measurements and secondary metabolite analysis were performed. The results showed that the fresh and dry weights of the shoot and root, leaf area, leaf length, and the number of leaves were decreased as increasing the microwave treatment times. Chlorophyll a and b, total carotenoids were increased and total phenolics were decreased at the 12-second treatment compared to the 4-second treatment. Total flavonoid contents were decreased at the 8-second treatment compared to the control. These results suggest that the changes in the levels of secondary metabolites were caused by oxidative stress. Although there was no significant difference in secondary metabolite contents excluding total flavonoid contents on the microwave treatments compared to the control, the significant difference suggests that the microwave treatment of 200 W and 2.45 GHz may alter secondary metabolite contents of lettuce after 4 weeks.