• Title/Summary/Keyword: southern gybridzation techniques

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Restriction endonuclease mapping of the plasmid pTi12 from agrobacterium tumefaciens (Agrobacterium tumefaciens KU-12 균주에서 분리한 플라스미드 pTi 12의 제한효소 지도)

  • 이용욱;손정훈;심웅섭
    • Korean Journal of Microbiology
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    • v.25 no.3
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    • pp.173-179
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    • 1987

  • Ti plasmids were isolated from three strains of Agrobacterium tumefaciens in Korea and their types and molecular weights were determined. All of these are octopine-type and their molecular weights are 44Kb (pTi 12), 180Kb (pTi 14) and 172Kb (pti 49), respectively. In order to construct physical map of pTi 12, pTi 12 was digested with restriction endonucleases Sma I and Hind III. Sma I degestion of pTi 12 produce 8 fragments and Hind III produced 10 fragments. Physical arrangements of these fragments was determined by Southern hybridization techniques.

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