• Development and Reproduction
• /
• v.15 no.1
• /
• pp.41-52
• /
• 2011

In the present study, embryoid bodies (EBs) obtained from induced pluripotent stem cells (iPSCs) were induced to differentiate into germ lineage cells by treatment with bone morphogenetic protein 4 (BMP4) and retinoic acid (RA). The results were compared to the results for embryonic stem cells (ESCs) and multipotent spermatogonial stem cells (mSSCs) and quantified using immunocytochemical analysis of germ cell-specific markers (integrin-${\alpha}6$, GFR-${\alpha}1$, CD90/Thy1), fluorescence activating cell sorting (FACS), and real time-RT-PCR. We show that the highest levels of germ cell marker-expressing cells were obtained from groups treated with 10 ng/$m{\ell}$ BMP4 or 0.01 ${\mu}M$ RA. In the BMP4-treated group, GFR-${\alpha}1$ and CD90/Thy-1 were highly expressed in the EBs of iPSCs and ESCs compared to EBs of mSSCs. The expression of Nanog was much lower in iPSCs compared to ESCs and mSSCs. In the RA treated group, the level of GFR-${\alpha}1$ and CD90/Thy-1 expression in the EBs of mSSCs Induced pluripotent stem cells, Mouse embryonic stem cells, Multipotent spermatogonial stem cells, Germ cell lineage, Differentiation potential. was much higher than the levels found in the EBs of iPSCs and similar to the levels found in the EBs of ESCs. FACS analysis using integrin-${\alpha}6$, GFR-${\alpha}1$, CD90/Thy1 and immunocytochemistry using GFR-${\alpha}1$ antibody showed similar gene expression results. Therefore our results show that iPSC has the potential to differentiate into germ cells and suggest that a protocol optimizing germ cell induction from iPSC should be developed because of their potential usefulness in clinical applications requiring patient-specific cells.

• Proceedings of the Korean Society for Agricultural Machinery Conference
• /
• 2000.11b
• /
• pp.210-218
• /
• 2000

Since the SSC(soluble solid contents) and titratable acidity of fruit are highly concerned to the taste, the need for measuring them by non-destructive technology such as NIR(Visual and Near-infrared) spectroscopy is increasing. Specially, in order to grade the quality of each fruit with a sorter at sorting and packing facilities, technologies for online measurement satisfying the tolerance in terms of accuracy and speed should be developed. Many researches have been done to develop devices to measure the internal qualities of fruit such as SSC, titratable acidity, firmness, etc. with the VIS(Visual)/NIR(Near Infrared) reflectance spectra. The distributions of the SSC, titratable acidity, firmness, etc. are different with respect to the position and depth of fruit, and generally the VIS/NIR light can interact with fruit in a few millimeters of pathlength, and it is very difficult to measure the qualities of inner flesh of fruit. Therefore, to measure the average concentrations of each quality factor such as SSC and titratable acidity with the reflectance-type NIR devices, the spectra of fruit at several positions should be measured. Recently, the interest about the transmittance-type VIS/NIR devices is increasing. NIR light can penetrate through the fruit about 1/10-1/1,000,000 %. Therefore, very intensive light source and very sensitive sensor should be adopted to measure the transmitted light spectra of intact fruit. The ultimate purpose of this study was to develop a device to measure the transmitted light spectra of intact fruit such as apple, pear, peach, etc. With the transmittance-type VIS/NIR device, the feasibility of measurement of the SSC and titratable acidity in intact fruit cultivated in Korea was tested. The results are summarized as follows; A simple measurement device which can measure the transmitted light spectra of intact fruit was constructed with sample holder, two 500W-tungsten halogen lamps, a real-time spectrometer having a very sensitive CCD array sensor and optical fiber probe. With the device, it was possible to measure the transmitted light spectra of intact fruit such as apple, pear and peach. Main factors affecting the intensity of transmitted light spectra were the size of sample, the radiation intensity of light source and the integration time of the detector. Sample holder should be designed so that direct light leakage to the probe could be protected. Preprocessing method to the raw spectrum data significantly influenced the performance of the nondestructive measurement of SSC and titratable acidity of intact fruit. Representative results of PLS models in predicting the SSC of peach were SEP of 0.558 Brix% and R2 of 0.819, and those in predicting titratable acidity were SEP of 0.056% and R2 of 0.655.

• The Mathematical Education
• /
• v.47 no.2
• /
• pp.197-219
• /
• 2008

Unlike ordinary situations, deifinitions play a very important role in mathematics education in schools. Mathematical concepts have been mainly acquired by given definitions. However, according to didactical intentions, mathematics education in schools has employed mathematical concepts and definitions with less strict forms than those in pure mathematics. This research mainly discusses definitions used in geometry (promising) course in primary schools to cope with possibilities of creating misconception due to this didactical transformation. After analyzing problems with potential misconceptions, a survey was conducted $\underline{with}$ 80 primary school teachers in Jeju to investigate their recognitions in meaning of mathematical concepts in geometry and attitudes toward teaching. Most of the respondents answered they taught their students while they knew well about mathematical definitions in geometry but the respondents sometimes confused mathematical concepts of polygons and circles. Also, they were aware of problems in current mathematics textbooks which have explained figures in small topics (classes). Here, several suggestions are proposed as follows from analyzing teachers' recognitions and researches in mathematical viewpoints of definitions (promising) in geometric figures which have been adopted by current mathematics textbooks in primary schools from the seventh educational curriculum. First, when primary school students in their detailed operational stage studying figures, they tend to experience $\underline{a}$ collision between concept images acquired from activities to find out promising and concept images formed through promising. Therefore, a teaching method is required to lessen possibility of misconceptions. That is, there should be a communication method between defining conceptual definitions and Images. Second, we need to consider how geometric figures and their elements in primary school textbooks are connected with fundamental terminologies laying the foundation for geometrical definitions and more logical approaches should be adopted. Third, the consistency with studying geometric figures should be considered. Fourth, sorting activities about problems in coined words related to figures and way and time of their introductions should be emphasized. In primary schools mathematics curriculum, geometry has played a crucial role in increasing mathematical ways of thoughts. Hence, being introduced by parts from viewpoints of relational understanding should be emphasized more in textbooks and teachers should teach students after restructuring this. Mathematics teachers should help their students not only learn conceptual definitions of geometric figures in their courses well but also advance to rigid mathematical definitions. Therefore, that's why mathematics teachers should know meanings of concepts clearly and accurately.

• Korean Journal of Veterinary Research
• /
• v.52 no.3
• /
• pp.183-191
• /
• 2012

The maintenance of peripheral immune tolerance and prevention of chronic inflammation and autoimmune disease require $CD4^{+}CD25^{+}$ T cells (regulatory T cells). The transcription factor Foxp3 is essential for the development of functional, regulatory T cells, which plays a prominent role in self-tolerance. Retroviral vectors can confer high level of gene transfer and transgene expression in a variety of cell types. Here we observed that following retroviral vector-mediated gene transfer of Foxp3, transductional Foxp3 expression was increased in the liver, lung, brain, heart, muscle, spinal cord, kidney and spleen. One day after vector administration, high levels of transgene and gene expression were observed in liver and lung. At 2 days after injection, transductional Foxp3 expression level was increased in brain, heart, muscle and spinal cord, but kidney and spleen exhibited a consistent low level. This finding was inconsistent with the increase in both $CD4^{+}CD25^{+}$ T cell and $CD4^{+}Foxp3^{+}$ T cell frequencies observed in peripheral immune cells by fluorescence-activated cell-sorting (FACS) analysis. Retroviral vector-mediated gene transfer of Foxp3 did not lead to increased numbers of $CD4^{+}CD25^{+}$ T cell and $CD4^{+}Foxp3^{+}$ T cell. These results demonstrate the level and duration of transductional Foxp3 gene expression in various tissues. A better understanding of Foxp3 regulation can be useful in dissecting the cause of regulatory T cells dysfunction in several autoimmune diseases and raise the possibility of enhancing suppressive functions of regulatory T cells for therapeutic purposes.

• 한국해양학회지
• /
• v.22 no.3
• /
• pp.143-152
• /
• 1987

The digital data were obtained using Kennedy 9000 magnetic tape deck which was connected to the SMS960 side scan sonar during the field operations. The data of three consecutive survey tracks near Seongsan-po, Cheju were used for the development of this study. The softwares were mainly written in Fortran-77 using VAX 11/780 MINI-COMPUTER (CPU Memory; 4MB). The established mapping system consists of the pretreatment and the digital processing of seafloor image data. The pretreatment was necessary because the raw digital data format of the field magnetic tapes was not compatible to the VAX system. Therefore the raw data were read by the personal computer using the Assembler language and the data format was converted to IBM compatible, and next data were communicated to the VAX system. The digital processing includes geometrical correction for slant range, statistical analysis and cartography of the seafloor image. The sound speed in the water column was assumed 1,500 m/sec for the slant range correction and the moving average method was used for the signal trace smoothing. Histograms and cumulative curves were established for the statistical analysis, that was purposed to classify the backscattering strength from the sea-bottom. The seafloor image was displayed on the color screen of the TEKTRONIX 4113B terminal. According to the brief interpretation of the result image map, rocky and sedimentary bottoms were very well discriminated. Also it was shown that the backscattered acoustic pressurecorrelateswith the grain size and sorting of surface sediments.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
• /
• v.4 no.2
• /
• pp.107-116
• /
• 1999

To study the characters of surface sediment and to describe the seasonal depositional environment as a result of sedimentation process off Byun-San Peninsula, a total 61 samples of surface sediment (32 samples in summer; 29 samples in winter) were collected and analysed. A digitized depth data from sea chart and echosounding profiles along five trans-sections were helpful for understanding the morphological factors. The types classified by the characters of surface sediment are type I (sand, S), type II (silty sand, zS), and type ill (sandy silt, sZ). Mean grain size varies from 2.11 to 7.81 ${\Phi}$. The positive-skewness shows the typical tide-dominated environment. The sediment type of the northwestern stations is medium sand and the sorting value is 0.5~1.4 ${\Phi}$ of well/moderately sorted. Meanwhile, other stations are composed of muddy sands and sandy muds transported from rivers and offshore. These sediment types toward inshore change gradually from silty sand to sandy silt. According to the C/M diagram, there are three major transport modes of sediment: bed load (Mode A), graded suspension (Mode B), and suspension (Mode C), correlating with north-eastern sandy area, middle part of silty-sand area, and southern sandy-silt area, respectively. The result of Principal Component Analysis shows also similar pattern of sediment types. In result, sediment texture of type III tends to be finer and more poorly-sorted than that of type II and sediment facies are correlateed with sedimentation process.

• Journal of Conservation Science
• /
• v.36 no.5
• /
• pp.315-325
• /
• 2020

The Daetongsa temple is the earliest temple to be constructed during the era of the Three Kingdoms in ancient Korea. The main architect, purpose, and name of the temple have been confirmed through ancient literature and archeological materials carved in the Chinese letter, Daetong, excavated around Gongju. However, the location and range of the temple have remained elusive and were discussed in various studies. In this study, we examine the roof tiles obtained from the presumed site of the Daetongsa temple. The tiles were found to contain traces of red paint (red pigments) on their surface and analyzed using nondestructive techniques. The results imply that roof tiles were made using clay tablets and wooden cylinders, with latticed cloth in between. Additionally, some wooden cylinders appeared to comprise numerous wooden plates tied together by strings. The clay tablets used to make the roof tiles were produced from the source clay via the sorting process. The traces of red paint on the surface of the roof tiles were verified to be traditional pigments used for painting wooden buildings. These pigments were extracted from red ocher or red clay (Seokganju), mainly consisting of iron oxide. In the literature, the location of provenance sites for Seokganju is estimated to be far from Gongju. However, the materials for extracting the red pigments were relatively easy to source because most rocks comprised iron oxides. Therefore, it is necessary to discuss the provenance of the red pigments around the presumed site of Daetongsa.

• Economic and Environmental Geology
• /
• v.53 no.4
• /
• pp.337-346
• /
• 2020

This study was carried out to evaluate the applicability of the soil washing process to remediation mercury-contaminated mine tailing or solid material (soil and sediments etc.) around abandoned mines. First, the physicochemical characteristics of mine tailing were analyzed through particle size analysis and sequential extraction. Secondly, laboratory scale washing experiments were performed using hydrochloric acid, nitric acid, potassium iodide and sodium thiosulfate. As a results of particle size analysis, mine tailing particle were concentrated below 40 mesh and the particle size below 200 mesh was the most analyzed. As a result of sequential extraction, elemental mercury fraction was analyzed as the highest with 69.12%, with strongly bound fraction 15.25% and residual and HgS fractions 11.97%, respectively. Laboratory scale washing experiments showed low applicability for nitric acid and sodium thiosulfate solutions. In case of hydrochloric acid solution, it was analyzed that mercury removal was possible at particle size of 200 mesh or more. Therefore, it is considered to be performed together with the physical sorting process. Potassium iodide solution was analyzed to have high washing efficiency at all concentrations and particle sizes. In particular, the mercury removal efficiency is high in the micro particles, and thus the applicability of the washing technology is the highest.

• Journal of Sensor Science and Technology
• /
• v.8 no.4
• /
• pp.341-346
• /
• 1999

In general cases, compared with glass electrode, FET type electrolyte sensors have many advantages. But the drift, memory effect and the poor reproducibility of the FET type electrolyte sensor cause the decrease of the reliability in the measurement system. To improve the reliability, an ion-measuring system using FET type electrolyte sensor array with 8 sensors has been developed. Developed system employed the electronic switchs to connect a signal detecting circuit with 8 sensor array and the system can measure ion concentration of 4 different type electrolyte($H^+$, $Na^+$, $K^+$, $Ca^{2+}$). The signal processing algorithm with insertion sorting method was adopted to enhance the reliability. We measured 3 different ion($H^+$, $Na^+$, $K^+$) to evaluate the performance of developed system. The results show that the designed signal processing algorithm can reduce the error range in comparison with a simple arithmetic mean and the developed system has a good reliability over the previous single channel sensor system.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.16 no.8
• /
• pp.5636-5645
• /
• 2015

Physical characteristics of aggregates affect the workability and strength of mortar and concrete, which include their fineness ratio, particle size distribution and water absorption. The workability of construction materials decreases if the incorporated fine aggregates show improper size distribution of their particles. This study shows the particle size effect on the rheological behavior of mortar and provides basic information for evaluating its workability. A mini-slump flow test was adopted to evaluate the workability of mortar. In addition, its plastic viscosity and yield stress were measured using a rheometer for building materials. The sand samples were prepared by sieving river sand and sorting out with their particle sizes. As a result, it was observed that the fines less than 0.7 mm increases the yield stress and plastic viscosity of the mortar samples. If the fines are less than 0.34 mm, the water absorption of the fines dominates change on the workability.