• Journal of the Society of Cosmetic Scientists of Korea
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• v.33 no.2
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• pp.127-135
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• 2007

This study was to evaluate the efficiency of different solvent systems to extract active ingredients from different parts of medicinal plants used as oriental medicinal herb in the cosmetic industry. We tested efficacies related to the antioxidative effects and UV absorption of herbal extracts as complex of active ingredients, not each single ingredient. When extracting medicinal plant which is used effective medicine, we should consider the collecting conditions like collecting time, place, part and extracting conditions like solvent, temperature, time, and etc. Among them, extraction solvent and collecting part are very important factors for the efficacy. As increased interest in medicinal plants, more intensive studies on collecting conditions and extracting processes were needed for the developments in the herbal ingredient industry. We evaluated the efficiency of different solvent systems to extract active ingredients from different parts of plants (Terminalia chebula, Syzygium aromaticum, Paeonia lactiflora, Morus alba, Scutellaria baicalensis) widely used as medicine and food. As results, we found that proper condition can make better data and decrease the required quantity.

• Journal of Ginseng Research
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• v.18 no.2
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• pp.122-127
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• 1994

Red ginseng extracts accounted for the major precipitating material in red ginseng drink prepared with various components and pasteuralized at $80^{\circ}C$ for 30 min. Precipitates in red ginseng extracts isolated by centrifugation were investigated for its chemical components and physicochemical properties. Recovery of precipitates in red ginseng extracts was 1.59% on a dry weight basis. Precipitates were composed of starch (71.47%), crude protein (21.75%), pectin (1.70%), polyphenol (2.97%) and calcium (3.83 ppm) but ginsenosides were not present. Absorption peak at 285 nm which appeared in red ginseng extracts was absent in the precipitates. pH and Hunter L value of the precipitates decreased, while Hunter a and b values increased in contrast to red ginseng extracts. Precipitates were solubilized to some extent in polar solvent. The shape of precipitate particle was round, sciral or irregular square. Particle size of precipitates varied from 0.17 $\mu\textrm{m}$ to 41.3 $\mu\textrm{m}$ in length.

• YAKHAK HOEJI
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• v.40 no.5
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• pp.582-590
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• 1996

For the research of antifungal active constituents from natural products, 226 plants were extracted with ether and methanol, separately, and screened antifungal activity against Candida albicans and Penicillium avellaneum cells. The results demonstrated that 30 samlpes showed antifungal activity in ether or methanol extracts and 17 samples in ether extracts and 20 samples in methanol extracts against C. albicans. Against P. avellaneum, 19 samlpes showed antifungal activity in ether or methanol extracts and 17 samples in ether extracts and 11 samples in methanol extracts, respectively. The antifungal activity of natural products against C. albicans were showed more sensitive than P. avellaneum, and the polarity of the solvent was not specific in antifungal activity.

• Journal of Physiology & Pathology in Korean Medicine
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• v.27 no.2
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• pp.189-195
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• 2013

This study was designed to analyze the effects of methanol extracts and solvent fractions of the young branch of Prunus persica on scavenging activity of ROS, RNS and inhibiting activity of oxidative modification in human LDL induced by copper ion or free radical generator. The scavenging capacities of the fractions on DPPH radical, superoxide radical, nitric oxide and peroxynitrite were exhibited the highest in ethylacetate fraction, followed by butanol fraction. In the copper-induced LDL oxidative modification system, the highest antioxidant activity was revealed in ethylacetate fraction, and butanol fraction exhibited a similar activity. However, solvent fractions of the young branch of Prunus persica showed a relatively low antioxidant activity in the AAPH-mediated LDL oxidation. In addition, ethylacetate and butanol fractions also inhibited the copper-mediated LDL oxidation in the REM assay, which was comparable to that of the positive controls, including EDTA, ascorbic acid and BHT. Futhermore, a content of total phenolics in these two fractions was higher than that of the other fractions. These results indicated that ethylacetate and butanol fractions of the young branch of Prunus persica were useful for the prevention of the free radical- or metal ion-induced oxidative damages.

• Food Science and Preservation
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• v.7 no.2
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• pp.195-200
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• 2000

Yields of various solvent extracts for Zanthoxylum schinifolium and Z. piperitum leaves were higher in ethanol extract layer. Ethanol Extract in sancho and chopi leaves was fractionated with different solvents, such as hexane, chloroform, ethyl acetate, butanol and water, yields of hexane fraction was higher. In the solvent extracts using methanol, ethanol, hexane, chloroform, and water, ethanol extract showed the most effective antimicrobial activities. Ethanol extract in sancho and chopi leaves was fractionated, the most antimicrobial activities of sancho leaf were ethyl acetate fraction, and chopi leaf were chloroform fraction.

• Korean Journal of Pharmacognosy
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• v.32 no.3 s.126
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• pp.175-180
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• 2001

Solvent extracts of 17 plants collected in Cheju Island were investigated for their biological properties related to cosmeceuticals such as tyrosinase and hyaluronidase inhibition and also radical scavenging effects. The chloroform fraction of Phytolacca esculenta root exhibited strong inhibition against tyrosinase activity. No fraction showed significant hyaluronidase inhibition. Some solvent extracts of plants such as Achyranthes japonica and Artemisia princeps showed considerable radical scavenging activities.

• Preventive Nutrition and Food Science
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• v.15 no.1
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• pp.36-43
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• 2010

The purpose of this study was to investigate the phenolic acid, proanthocyanidin (PAs), and flavonol glycoside contents, as well as the antioxidant activities of pine needle extracts from six species of young pine trees. The extracts were prepared from Section Pinus (Diploxylon): P. densiflora, P. sylvestris, P. pinaster and P. pinea, and Section Strobus (Haploxylon): P. koraiensis and P. strobus. Phenolics were extracted from pine needles with 80% acetone to obtain the soluble free fraction, and insoluble residues were digested with 4 M NaOH to obtain bound ethyl acetate and bound water fractions. Phenolics were analyzed by HPLC, and the hydrophilic antioxidant activity was measured using oxygen radical absorbance capacity (ORAC). Total phenolic and flavonoid contents of the soluble free fraction were higher than those of the bound ethyl acetate and bound water fractions. The main phenolics were monomers and polymers of PAs in the soluble free fraction, and phenolic acids and flavonol glycosides in bound ethyl acetate fraction. Flavonol glycosides found in different species of pine needles were qualitatively similar within fractions, but composition varied among Pinus sections. High levels of kaempferol arabinoside and an unknown compound were present in all Strobus species. The soluble free fraction had the highest antioxidant activity, followed by bound ethyl acetate and bound water fractions.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.20 no.2 s.33
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• pp.36-46
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• 2007

Objective : In this study, the effect of Atractylodes japonica against LPS induced inflammation in mouse microglia BV2 cells was investigated. Method : Microglia BV2 Cells viability was determined using the MTT assay. We used water, ethanol extract from Atractylodes japonica and studied on the anti-inflammatory effect of lipopolysaccharide-induced inflammation using reverse transcription polymerase chain reaction (RT-PCR), western blot, and nitric oxide detection on mouse microglia BV2 cells. Result : The MTT assay revealed that it's extract has no significant cytotoxicity in the microglia BV2 cell. Various solvent extract from Atractylodes japonica inhibited nitrite production, iNOS protein and mRNA expression levels. And also it's extracts significantly reduced lipopolysaccharide-induced COX-2 activation in RT-PCR and western blot in lipopolysaccharide-induced microglia BV2 cells Conclusion : In this study, it's extracts was shown to suppress NO production by inhibiting iNOS expression and COX-2 activity. With this effects of anti-inflammation, we suggests that, it's extracts may be a useful candidate for the development of a drug on the related inflammatory diseases in brain.

• The Korean Journal of Food And Nutrition
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• v.26 no.4
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• pp.814-823
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• 2013

This study is carried out to evaluate the genotoxicity of herbs (Angelica decursiva, Polypori umbellate, Astragalus membranaceua, Paeonia iactiflora, Glycyrrhiza uralensis, Cnidium officinale, Rehmannia glutinosa, Cyperus rotundus, Dioscorea batatas and Platycodi Radix) by using the Ames test. The Salmonella typhimurium reversion assay is being performed by using the Sal. typhimurium TA98, TA100, TA102, TA1535 and TA1537 as tester strains. Among 70% of ethanol extracts from 10 herbs, the number of revertant colonies is being increased in Astragalus membranaceua, Cnidium officinale and Dioscorea batatas in a dose-dependent manner, as compared with negative controls of the metabolic activation. In case of dichloromethane and ethyl acetate fraction from 70% of ethanol extracts, the number of revertant colonies is increased in Angelica decursiva, Astragalus membranaceua, Cnidium officinale, Rehmannia glutinosa and Dioscorea batatas in a dose-dependent manner, as compared with negative controls of metabolic activation. Such results indicate that Angelica decursiva, Astragalus membranaceua, Cnidium officinale, Rehmannia glutinosa and Dioscorea batatas all show genotoxic effects when being extracted with the solvent extractions such as 70% of ethanol, dichloromethane and ethyl acetate, and thus, they might be genotoxically- non-safe.

• The Korean Journal of Food And Nutrition
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• v.30 no.1
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• pp.51-58
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• 2017

Antioxidant activities of 80% methanol, water, and 70% ethanol extracts of Lentinula edodes GNA01 were compared and estimated. The yield of Lentinula edodes GNA01 was identified to be in the following order: water>70% ethanol>80% methanol, but there was no significant difference between 80% methanol and 70% ethanol extracts. The highest total phenolic content (TPC) and total flavonoid content (TFC) were found in water extract, and TPC of 80% methanol extract was higher than that of 70% ethanol extract and TFC of 70% ethanol extract was higher than that of 80% methanol extract. Water extract exhibited the strongest DPPH, ABTS radicals, and nitrite scavenging activities, $Fe^{2+}$ chelating ability, and FRAP among the three extracts. In addition, antioxidant activity of 80% methanol extract was higher than that of 70% ethanol extract in most of the experiments. As a result, antioxidant activity of Lentinula edodes GNA01 showed a difference according to extraction solvent and concentration; nevertheless, water extract exhibiting high polarity had the strongest antioxidant effect. Consequently, water extract from Lentinula edodes GNA01 is anticipated to be useful for the development of a high value-added functional product.