• Title/Summary/Keyword: solution culture

Search Result 1,306, Processing Time 0.024 seconds

Influence of pretreatment medium, fresh medium addition, and culture plate size on the production of embryos in isolated microspore culture of hot pepper (Capsicum annuum L.) (고추의 소포자 배양 시 전처리 배지, 새 배지의 첨가, 및 배양 용기의 크기가 배의 생산에 미치는 영향)

  • Park, Eun-Joon;Kim, Jin-Ae;Kim, Moon-Za
    • Journal of Plant Biotechnology
    • /
    • v.36 no.2
    • /
    • pp.184-192
    • /
    • 2009
  • The influences of pretreatment medium, the addition of fresh medium, and the size of culture plate on the production of embryos were investigated in isolated microspore culture of hot pepper (Capsicum annuum L.). Among the media used for heat shock pretreatment ($32{\pm}1{^{\circ}C}$), high frequency embryo production was obtained when the sucrosestarvation medium A was used. On the other hand, neither 0.37 M mannitol solution nor NLNS medium supplemented with sucrose was not efficient for embryo production. The addition of culture medium to pretreatment media considerably decreased the embryo production even though embryo development proceeded further. The embryo production was not improved by the addition of fresh medium after 2 or 3 weeks from starting culture. Increase in the size of the culture plate from $3.5{\times}1.0$ cm to $6.0{\times}1.5$ cm improved embryo quality. These results will provide valuable information for developing an efficient microspore culture system of hot pepper for high frequency embryo production.

An Analysis of Influence of Organizational Structure and Corporate Culture (경영조직구조(經營組織構造)와 기업문화(企業文化)의 관련성(關聯性)에 관한 연구(硏究))

  • Heo Kap-Soo
    • Management & Information Systems Review
    • /
    • v.11
    • /
    • pp.1-21
    • /
    • 2002
  • Corporate culture is being recognized as essential managerial resources in the future business management and in recently the attempts, though weak, of developing the corporate culture which is corresponded with their own structure and needs are being carried on by some major companies. Then, corporate culture research, drawing attention in nowadays, indicates that it attempts to analyze and search the organization matters in the basical dimension in regard to the assumptions, values and beliefs of organization and provides the solution of organizational negative effects, which have not been settled in the previews research methods of organizational behavior, and that present turbulent management environment demands the whole conversion from the previews hard style to the soft managerial style. For this reason, this study aim at identifying organizational structure and corporate culture in the business group. Futhermore, the influence of organizational structure and organizational culture are examined. The objective of this paper attempts to analyze the ways and results of strong and special culture on the organizational structure and culture. To accomplish this purpose, this study gathers in-depth data form various secondary courses. First, uncertainties in market and technology are the main sources of mimetic isomorphism. Due to the high level of uncertainty is market and technologies. Smallness in size is responsible for the low level of complexity in organizational structure. The rapid changes in market, contents of service, and tastes of customers are responsible for the low level of formalization, which enhances swift decision making and responses.

  • PDF

Effects of Compost Leachate on Growth and Yield of Leaf Lettuce in Hydroponic Culture (퇴비단 여과액비가 수경재배에서 상추의 생육과 수량에 미치는 영향)

  • Ryoo, Jong-Won
    • Journal of Animal Environmental Science
    • /
    • v.15 no.1
    • /
    • pp.51-58
    • /
    • 2009
  • The compost leachate was dark-colored solution that leaches out of the bottom of the compost pile. The compost leachate was rich in nutrients and can potentially used in plant culture. In the organic production, commercial liquid fertilizer was used to insure the availability of nutrients during the formation of the yield. The cost of supplemental liquid fertilizer could be reduced by developing a fertilizer based on animal fertilizer. This experiment was conducted to investigate the effect of different combination of compost leachate and conventional inorganic solution in hydroponic culture for lettuce growth. Six different treatments were applied. The compost leachate(CL) and nutrient solution(NS) were mixed by six different mixing ratios of 0:100, 20:80, 40:60, 60:40%, 80:20 and 100%:0% based on nitrogen content. The chemical nutrient solution was the solution of National Horticulture Research Station for the growth of leaf lettuce. The concentration of nutrient solution was adjusted 1.5 mS/cm in EC. The compost leachate was low in phosphorus(P), calcium(Ca), magnesium(Mg), but rich in potassium(K). The plant height of lettuce treated with CL 20+NS 80% was similar with 100% NS of control plot. Plant height was highest in the plot of CL 20+NS 80%. The treatment of 100% compost leachate was lowest in the growth characteristics of leaf lettuce. Number of leaf was very low in 100% compost leachate compared with plot of chemical nutrient solution. In the beginning of growth stage, SPAD value was reduced in plot treated with CL100%, but CL20+NS80% plot was higher compared to 100% compost leachate. SPAD value of leaf lettuce leaves was decreased as the amount of CL was increased. The dry weight of lettuce were 107.4, 104.2g in plot of NS 100% and CL 20%+NS80%, respectively. The leaf number and plant weight were decreased at high application plots of compost leachate. The leaf lettuce showed lowest in the plot treated with 100% compost leachate, and the growth of lettuce severely decreased after application of 100% CL treatment. The results showed that compost leachate can be use as liquid fertilizer for the organic hydroponic production. The mixture solution of 20% of compost leachate and 80% of nutrient solution could be used as a nutrition solution in hydroponic culture of leaf lettuce. Our studies have shown that is possible to produce using compost leachate, although growth is slower than when using a conventional inorganic hydroponic solution.

  • PDF

A Study on Dyeability of Cotton Fabrics using Ginseng Extracts (인삼 추출물 처리에 의한 천연 염색 면직물의 기능성 연구)

  • Kim, Wol-Soon
    • The Research Journal of the Costume Culture
    • /
    • v.19 no.2
    • /
    • pp.324-333
    • /
    • 2011
  • This study was accomplished for the purpose of developing a textile processing ingredient that is harmless to the human body and environment. The research method consists of dyeing cotton textiles by extracting the dye solution from ginseng. Then, chrominance, after treatment, antibacterial ratio and deodorization ratio of cotton fabrics dyed with ginseng extracts were tested and results were examined. The research procedure involved first extracting the dye solution from the ginseng's by-product (fine roots) and then dyeing was effectuated differently according to the test samples temperature and dyeing time requirements. Brightness in all dye substances was lower in pre-mordanting. Beige color could be extracted from pre-mordanted samples. And dark orange from postmordanted samples. Color-festness was high in all samples. Most of samples show a big antibacterial ratio and deodorization ratio. Through this research it has been discovered that, when applied to textiles, Korea's ginseng extract possessed reproducibility features as a natural dye and a possibility to be used in cutting which plays a crucial role in hygienic processing. In addition, by using ginseng's by-product for dyeing processing as the dye solution, efficient application of resources and occurrences of no water waste damages were demonstrated and thus, proved to be environmentally-friendly. Specifically, through this experiment, it was found that saponin, ginseng's special characteristics, possessed excellent antibacterial odor repelling functions to clothing as well as the capability to prevent skin disease.

Effects of Water Stress on Germination in Winter Barley (수분부족이 보리의 발아에 미치는 영향)

  • 백형진;최원열
    • KOREAN JOURNAL OF CROP SCIENCE
    • /
    • v.39 no.2
    • /
    • pp.139-145
    • /
    • 1994
  • This study was intended to obtain the basic information on the germination status, changes of $\beta$-amylase activity and reducing sugar contents during seed germination of barley after an imposition of water stress by adding Polyethylene glycol solution. The effects of water deficit on germination were to be decreased in germination rate and velocity. Respiratory Quotient was increased as the osmotic potential in culture solution was decreased. But $\beta$-amylase activity and reducing sugar contents were decreased and the ratio of dry to fresh weight of germinated seeds was increased as the osmotic potential of the culture solution was decreased.

  • PDF

Removal Efficiency of Residual Pesticides During Processing of Perilla Jangachi preparation (깻잎장아찌 제조과정 중의 잔류농약 제거 효과 연구)

  • Nam, Sang-Min;Lee, Hye-Ran;Lee, Jong-Mee
    • Journal of the Korean Society of Food Culture
    • /
    • v.18 no.6
    • /
    • pp.562-568
    • /
    • 2003
  • This study was performed to study the removal efficiency of residual organophosphorus pesticides with process for making Perilla Jangachi. Two organophosphorus pesticides(chlorpyrifos-methyl and fenitrothion) were artificially attached to Perilla leaves. Then Perilla leaves were washed with detergent solution for 1minute and rinsed 2 times each for 1 minutes. After washing with neutral detergent solution, Perilla Jangachi was made with 2 steps of optimal condition. As a pretreatment, when soaked with 2% salt concentration solution for 42hours, the removal rate of residual pesticides was 81.75% of chlorpyrifos-methyl and 76.82% of fenitrothion. When Perilla leaves were steamed for 72 seconds after soaking, it became 88.94% and 82.19%, respectively. Finally, after making optimal Perilla Jangachi with 27% onion contents, removal rate was 89.12% of chlorpyrifos-methyl and 82.76% of fenitrothion. Consequently, it appeared that the process for making Perilla Jangachi effectively removed the residual pesticides of Perilla leaves.

Mechanical and Electrical Properties of Aluminium Alloy by Cryorolling Process

  • Wangkasem, P.;Rojananan, S.
    • International Journal of Advanced Culture Technology
    • /
    • v.3 no.1
    • /
    • pp.46-51
    • /
    • 2015
  • The mechanical and electrical properties of aluminium alloy 6061 are reported in this present work. Aluminium alloys were homogenized at $550^{\circ}C$, for 5 hours and cooled in the furnace. The different thickness reductions of 60-90% on homogenized aluminium alloy plates were achieved by cryo-rolling. Later, the as rolled samples were aged by solution treatment at the temperatures of $520^{\circ}C$ for 1 hour, water quenched; subsequently aged at $160^{\circ}C$ for 8-24 hours and partial aged (not solution treatment) at $160^{\circ}C$ for 8- 24 hours. Mechanical and electrical properties of samples were investigated. The experimental result showed that the microhardness of cryo-rolled samples were increase with increasing the percentage of the thickness reduction. Moreover, the microhardness of cryo-rolled, aged by solution treatment samples were higher than those of the cryo-rolled and cryo-rolled, partial aged samples. The cryo-rolled alloys subjected to full aged at $160^{\circ}C$ for 24 hours exhibited the hardness of 125 HV and electrical conductivity values was 45.76 %IACS and the cryo-rolled alloys subjected to partial aged at $160^{\circ}C$ for 20 hours exhibited the hardness of 67 HV and electrical conductivity values was 49.67 %IACS.

Effect of Sodium Chloride on Weight Loss of AA1100 Aluminum Alloy and SGACD Zinc coated Steel Lap Joint

  • Maulidin, Achmad;Kimapong, Kittipong
    • International Journal of Advanced Culture Technology
    • /
    • v.3 no.1
    • /
    • pp.39-45
    • /
    • 2015
  • This research aims to study an effect of sodium chloride solution concentration on the corrosion rate of AA1100 aluminium alloy and SGACD zinc coated steel lap joint with a test duration of 30 days and a test temperature of $45^{\circ}$. The summarized results are as follows. Increase of the NaCl solution concentration increased the weight loss of Al, corrosion rate of Al, weight loss of Fe and also decreased the corrosion rate of Fe. Increase of the test duration affected to increase the weight loss and corrosion rate of Al and also decrease the weight loss and corrosion rate of Fe. The corrosion that was formed in a lap joint consisted of the uniform corrosion on the surface of the metals and the galvanic corrosion in the lap area of the joint. The maximum weight loss of AA 1100 aluminium and SGACD zinc coated steel that was occurred in the sodium chloride with 3.25% was 2.203% and 3.208%, respectively.. The maximum corrosion rate of AA 1100 aluminium and SGACD zinc coated steel that was occurred in 4.00% and 3.5% sodium chloride solution was 0.156 mm/year and 0.479 mm/year, respectively.

Effect of nitrogen types and the electrical conductivity of a nutrient solution on gray mold caused Botrytis cinerea on strawberry plants

  • Nam, Myeong hyeon;Lee, Hee chul;Kim, Tae il
    • Korean Journal of Agricultural Science
    • /
    • v.46 no.1
    • /
    • pp.103-111
    • /
    • 2019
  • Gray mold caused by Botrytis cinerea on strawberry plants is an economically significant disease in Korea. The rates for diseased fruits are high during the strawberry harvesting period from December to February, especially in hydroponic cultivation. This study assessed the effect of the nitrogen type in the soil culture and the electrical conductivity (EC) of the nutrient solution in a hydroponic culture on the gray mold incidence in 'Seolhyang' strawberry plants. The nitrogen sources assayed included calcium nitrate tetrahydrate (CN4), calcium nitrate decahydrate (CN10), ammonium sulfate (AS), and commercial fertilizer 213 (213). The effect of the EC was tested at 0.5, 0.8, 1.0, and $1.5dS{\cdot}m^{-1}$. The occurrence of gray mold varied according to the nitrogen type. The disease incidence and nitrogen content for the main nitrogen type were higher compared to the non-treated control. The AS treatment showed the highest occurrence of tipburn and gray mold. The incidence of gray mold as well as the nitrogen and phosphorus content of the leaves increased as the EC level was increased. These results indicate that the incidence of gray mold in strawberry plants is related to the nitrogen content of the leaf and the EC of the nutrient solution.

Inhibitory effects of Arisaematis rhizoma(天南星) on cell proliferation in HeLa cell (천남성(天南星)이 HeLa Cell의 증식억제(增殖抑制)와 apoptosis에 미치는 영향(影響))

  • Cho, Jung-Hoon;Jang, Jun-Bock;Lee, Kyung-Sub;Bae, Woo-Jin
    • The Journal of Korean Obstetrics and Gynecology
    • /
    • v.19 no.3
    • /
    • pp.25-40
    • /
    • 2006
  • Purpose : This study was undertaken to evaluate the inhibitory effects of Arisaematis rhizoma on the cell proliferation in HeLa cells. Methods : The cultured cell after treatment in the different duration in 24, 48, 72 hours with solution of 1%. 5%, 10% Arisaematis rhizoma was quantified by trypan blue exclusin method. The control group was treated with 2% FBS in the different duration in 24, 48, 72 hours. We examined DNA of activated caspase by FACS analysis, caspase-3 activity, DNA fragmentation by DNA laddering, activity of HeLa Cells by the XTT assay, activity of MAP kinase by RT-PCR analysis. Results : After 72 hours culture, the growth activities of 1%, 5%, 10% Arisaematis rhizoma-treated Hela cell were significantly reduced with control group, respectively. After 24 hours culture, the ratio of cells showing caspase activity by FACS analysis were increased in 1%, 5%, 10% Arisaematis rhizoma-treated Hela cell. It were also increased in 48 hours culture of 10% and 72 hours culture of 5%, 10% Arisaematis rhizoma-treated Hela cell. In 24, 48 and 72 hours culture, DNA fragmentations of 5%, 10% Arisaematis rhizoma-treated Hela cell were obviously observed. These results meaned that Arisaematis rhizoma induces apoptosis of HeLa cells. It was supported by increased caspase-3 activity and decreased MAP kinase activity according to time periods and concentrations of Arisaematis rhizoma solution. Conclusion : The study shows that Arisaematis rhizoma has inhibitory effect on cell proliferation and induction capacity of apoptosis of human cevical carcinoma cell line, HeLa cells, in vitro. These results suggest that Arisaematis rhizoma should be useful for treatment of human cevical carcinoma.

  • PDF