• Korean Journal of Food Science and Technology
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• v.40 no.6
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• pp.680-685
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• 2008

In this study, we assessed the functional characteristics of Aralia continentalis Kitagawa (AcK) and Aralia continentalis Kitagawa leaf (AcK-leaf), including crude saponin contents, antioxidant activity, nutritive elements, dietary fiber, and chlorophyll contents. The results of our analysis of nutritive elements in AcK, the contents of the general components, inorganic components, and vitamins were all relatively high. The contents of inorganic components were also high in the following order: K>Ca>P>Na>Mg. Among vitamins, $\beta$-carotene and vitamin C contents were all high. The crude saponin content was 63.7 mg/g in the AcK and 63.5 mg/g in the AcK leaf, and the antioxidant activity was determined to be relatively high. With regard to the dietary fiber content, the total dietary fiber content was 2.13% (soluble 0.40, insoluble 1.72) in the AcK and 5.98% (soluble 1.06, insoluble 4.89) in the AcK leaf, and the total chlorophyll content was 92.58 mg in the AcK and 147.25 mg/100 g in the AcK-leaf.

• Journal of Microbiology and Biotechnology
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• v.8 no.5
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• pp.509-516
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• 1998

A plasmid vector, pXGPRMATG-lac-Tgx, was developed for overproduction of $\beta$-galactosidase in Escherichia coli using the genetic components of groEx, a heat-shock gene cloned from symbiotic X-bacteria in Amoeba proteus. The vector is composed of intragenic promoters P3 and P4 of groEx, the structural gene of lac operon, transcription tenninator signals of lac and groEx, and ColEl and amp'of pBluescript SKII. The optimized host, E. coli DH5$\alpha$, transfonned with the vector constitutively produced 117,310-171,961 Miller units of $\beta$-galactosidase per mg protein in crude extract. The amount of enzyme in crude extract was 53% of total water-soluble proteins. About 43% of the enzyme could be purified to a specific activity of 322,249 Miller units/mg protein after two-fold purification, using two cycles of precipitation with ammonium sulfate and one step of gel filtration. Thus, the expression system developed in this study presents a low-cost and simple method for purifying overproduced $\beta$-galactosidase in E. coli.

• Asian-Australasian Journal of Animal Sciences
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• v.16 no.4
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• pp.511-514
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• 2003

Previously, we reported that a diet including sesame meal (SM) increased plasma total and high-density lipoprotein (HDL)-cholesterol concentrations in goats. In the present study, the components in the sesame meal that can increase plasma total and HDL-cholesterol concentrations have been examined. In experiment 1, we gave goats defatted sesame meal diet (DSM) to investigate the influence of ether extract fraction remained in sesame meal. Corn gluten meal diet (CGM) was also fed to goats as a high-protein diet to examine the influence of high dietary protein level caused by usage of sesame meal. Plasma total and HDL-cholesterol concentrations of goats fed DSM and CGM did not change during experimental periods though they were elevated by feeding SM. In experiment 2, the influence of sesame oil and corn oil added in diets on plasma total and HDL-cholesterol concentrations in goats was investigated. Plasma total and HDL-cholesterol concentrations were increased by feeding both corn oil diet and sesame oil diet. In conclusion, the increase in plasma HDL-cholesterol concentration by feeding sesame meal was resulted by the effect of ether extract fraction including sesame oil or some lipid-soluble components remained in sesame meal.

• Korean Journal of Food Science and Technology
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• v.36 no.4
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• pp.563-567
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• 2004

Nutritional components, such as approximate compositions, and amino acid, mineral, vitamin, sugar, and fatty acid contents, of artificially cultivated Pleurotus ferulea were analyzed. Contents of carbohydrates, crude lipids, dietary fibers, crude proteins, total amino acids, particularly essential amino acids, minerals, water-insoluble and-soluble vitamins, glucose, and unsaturated fatty acids such as linoleic acid of P. ferulea were higher than those of P. ostreatus and P. eryngii. Results indicate P. ferulea has abundant essential nutrients and thus is good source of functional healthy food.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.36 no.5 s.108
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• pp.62-68
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• 2004

The amount of non-lignin components in unbleached and oxygen-delignified kraft pulps and their impact on lignin determinations was investigated. The lignin analyses investigated were kappa number and Klason lignin in conjunction with acid-soluble lignin. The species investigated were loblolly pine, and aspen. The non-lignin components that impacted on lignin determination were residual extractives and hexenuronic acid in unbleached and oxygen-delignified kraft pulps. In the hardwoods, significant amounts of extractives remained after kraft pulping and oxygen delignification. These residual extractives in the hardwood pulps had an impact on the lignin determination, more so on the acid lignin method than kappa number. Hexenuronic acid only impacts on kappa number determination both softwood and hardwood pulps, not on acid lignin. Hexeneuronic acid contributed as lignin content more in aspen than pine pulps, and more in oxygen-delignified than unbleached kraft pulps. Impact of hexenuronic acid on should be corrected both softwood and hardwood pulps for accurate kappa number.

• Journal of Microbiology
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• v.40 no.4
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• pp.260-266
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• 2002

Bacterial strains were isolated from biofilms formed on glass slides submerged in seawater in Dae-Ho Dike. Eight strains showing fast attaching ability were selected and identified. Their exopolysaccharide (EPS)-producing ability and EPS properties were characterized. Based on Microlog System, 4 among the 8 strains were identified as Micrococcus luteus and the rest were Bacillus thuringiensis, Bacillus megaterium,, Staphylococcus saprophyticus and Agrobacterium vitis. A, vitis was reidentified as Sulfitobacter pontiacus based on 16S rDNA sequence data. The amount of water-soluble EPS produced by the 8 strains ranged from 0.114 to 1.329 g$.$l$\^$-1/ and the productivity was negatively correlated with the cell biomass. The molecular weight of the produced EPS ranged from 0.38 to 25.19$\times$10$\^$4/ Da. Glucose and galactose were ubiquitous sugar components. Mannose, ribose, and xylose were also major sugar components. The molecular weight and composition of the EPS showed strain-specific variation.

• Applied Biological Chemistry
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• v.36 no.3
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• pp.154-157
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• 1993

Based upon the antioxidative effectiveness of ether extracts of defated Crataegus pinnatifida B., phenolic antioxidative components were separated by gel column chromatography and identified by MS and H-NMR. Two or three individual compounds were found in free, soluble and insoluble bound phenolic acids, respectively and they were identified as caffeic acid, protocatechuic acid, phloroglucinol and pyrogallol.

• Journal of Plant Biology
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• v.31 no.4
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• pp.277-288
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• 1988

The effects of ammonium ion and glutamate on CO2 fixation abilities and related carbon metabolism were investigated in pea (Pisum sativum L. cv. Sparkle) leaf discs under conditions favoring photorespiration (21% O2, 0.03% CO2) and nonphotorespiration (5% O2, 0.03% CO2). A concentration of more than 10 mM of NH4+ decreased the photosynthetic CO2 fixation and those inhibitory effects were more remarkable in 21% O2 than in 5% O2 conditions. The effect of glutamate on CO2 fixation was found to be independent of the O2 level, as glutamate increased the CO2 fixation under both 21% and 5% O2 conditions. L-methionine-dl-sulfoximine, an irreversible inhibitor of glutamate synthetase, however, inhibited the CO2 fixation markedly under 21% O2, but did not affect it under 5% O2 conditions. The treatment with NH4+ elevated the relative amounts of 14C incorporated into soluble components from 14CO2 with no relation to O2 levels, while glutamate increased 14C into insoluble components and neutral sugars. Glutamate, especially, seemed to stmulate the biosynthesis of starch under 5% O2 condition. These results indicated that NH4+ stimulated the degradation of sugar or starch and this proposal was confirmed by the increasing of pyruvate kinase activity in leaf discs treated with ammonium ion.

• Journal of Plant Biology
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• v.20 no.2
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• pp.83-89
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• 1977

Changes in the alcohol-soluble free amino acids during germination of a spring grain, Wanju and two winter grains, Sedohadaka and Nonsankwa No.1-6 which are differing in their degree of spring (winter) habits, were investigated by thin layer chromatography. The results obtained are as follows. 1. Throughout the germination period, 25 ninhydrin positive components; 22 amino acids including two amides and 3 unknown spots were detected. It is interesting to note that identification of histidine was confined to Wanju and Sedohadaka but Nonsankwa No.1-6, which has the lower degree of spring habit. 2. Except the quiescent seeds, the major components were generally composed of the acidic and neutral amino acids together with glutamine and asparagine. 3. Proline was contained in higher quantity except from the stage of quiescent seeds, but the outstanding difference among the varieties was not recognized. Whether this component is related to the mechanism of spring habit in barley or not is a problem to be studied more. 4. In all the varieties, most of the changes in amino acid levels during germination were usually in the same direction and of the same pattern under the temperature controlled not to be varnalized. In view of the results above, the changes in the amino acid levels seem to be more affected by the changes of growing circumstances and the subsequent metabolic activities of certain enzymes than by the characteristics of varieties themselves.

• Particle and aerosol research
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• v.5 no.2
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• pp.45-52
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• 2009

Black carbon, which is a by-product of combustion of fossil fuel and biomass burning, is the component that imposes the largest uncertainty on quantifying aerosol climate effect. The direct, indirect and semi-direct climate effects of black carbon depend on its state of the mixing with other water-soluble aerosol components. The process that transforms hydrophobic externally mixed black carbon particles into hygroscopic internally mixed ones is called "aging". In most climate models, simple parameterizations for the aging time scale are used instead of solving detailed dynamics equations on the aging process due to the computation cost. In this study, a new parameterization for the black carbon aging time scale due to condensation and coagulation is presented as a function of the concentration of hygroscopic atmospheric components and the black carbon particle size. It is shown that the black carbon aging time scale due to condensation of sulfuric acid vapors varies to a large extent depending on the sulfuric acid concentration and the black carbon particle size. This result indicates that the constant aging time scale values suggested in the literature cannot be directly applied to a global scale modeling. The aging time scale due to coagulation with internally mixed aerosol particles shows an even stronger dependency on particle size, which implies that the use of a particle-size-independent aging time scale may lead to a large error when the aging is dominated by coagulation.