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Development of Method using LC-ESI-MS/MS and KASP for Identification of Gymnema sylvestre in Food (식품에서 당살초 판별을 위한 LC-ESI-MS/MS 분석법과 KASP 마커 개발)

  • Park, Boreum;Lee, Sun Hee;Eom, Kwonyong;Noh, Eunyoung;Moon Han, Kyoung;Hwang, Jinwoo;Kim, Hyungil;Baek, Sun Young
    • Journal of Food Hygiene and Safety
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    • v.37 no.2
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    • pp.46-54
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    • 2022
  • Known for its effectiveness in weight loss and diabetes prevention, Gymnema sylvestre products can be found in the US, Japanese, and Indian markets. However, the recommended dosage or safety of these products has not yet been proven. Therefore, development of an analytical method for detecting the content of Gymnema sylvestre in food products is required. Accordingly, this study proposes an analysis method that can examine Gymnema sylvestre in food using LC-ESI-MS/MS and KASP (Kompetitive Allele-Specific PCR) markers. In LC-ESI-MS/MS, a simultaneous analysis method for gymnemic acid and deacylgymnemic acid was optimized using negative ionization mode, and its validation test was completed for solid and liquid samples. In addition, KASP markers were prepared by finding the specific SNP of G. sylvestre in ITS2 and matK through DNA barcodes. The two KASP markers returned positive FAM fluorescence result when combined with G. sylvestre, and this aspect was confirmed in raw G. sylvestre as well. The applicability of the method was tested on 21 different food and healthy functional products containing G. sylvestre purchased on the internet. As a result, although there was a difference in the ratios of gymnemic acid and deacylgymnemic acid in LC-ESI-MS/MS, the index component was detected in all 21 products samples. In the KASP analysis, 9 products returned positive FAM result, and the rest of the products were found to be containing G. sylvestre extract. This study is the first study to use the dual system of LC-ESI-MS/MS and KASP for the analysis of G. sylvestre. The study has confirmed that these two methods are applicable to the examine G. sylvestre content in food products.

Optimization and Application Research on Triboelectric Nanogenerator for Wind Energy Based High Voltage Generation (정전발전 기반 바람에너지 수확장치의 최적화 및 고전압 생성을 위한 활용 방안)

  • Jang, Sunmin;Ra, Yoonsang;Cho, Sumin;Kam, Dongik;Shin, Dongjin;Lee, Heegyu;Choi, Buhee;Lee, Sae Hyuk;Cha, Kyoung Je;Seo, Kyoung Duck;Kim, Hyung Woo;Choi, Dongwhi
    • Korean Chemical Engineering Research
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    • v.60 no.2
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    • pp.243-248
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    • 2022
  • As the scope of use of portable and wearable electronic devices is expanding, the limitations of heavy and bulky solid-state batteries are being revealed. Given that, it is urgent to develop a small energy harvesting device that can partially share the role of a battery and the utilization of energy sources that are thrown away in daily life is becoming more important. Contact electrification, which generates electricity based on the coupling of the triboelectric effect and electrical induction when the two material surfaces are in contact and separated, can effectively harvest the physical and mechanical energy sources existing in the surrounding environment without going through a complicated intermediate process. Recently, the interest in the harvest and utilization of wind energy is growing since the wind is an infinitely ecofriendly energy source among the various environmental energy sources that exist in human surroundings. In this study, the optimization of the energy harvesting device for the effective harvest of wind energy based on the contact electrification was analyzed and then, the utilization strategy to maximize the utilization of the generated electricity was investigated. Natural wind based Fluttering TENG (NF-TENG) using fluttering film was developed, and design optimization was conducted. Moreover, the safe high voltage generation system was developed and a plan for application in the field requiring high voltage was proposed by highlighting the unique characteristics of TENG that generates low current and high voltage. In this respect, the result of this study demonstrates that a portable energy harvesting device based on the contact electrification shows great potential as a strategy to harvest wind energy thrown away in daily life and use it widely in fields requiring high voltage.

Evaluation of the Potential of Nitrogen Plasma to Cosmetics (질소 플라즈마의 화장품 가능성 평가)

  • Lee, So Min;Jung, So Young;Brito, Sofia;Heo, Hyojin;Cha, Byungsun;Lei, Lei;Lee, Sang Hun;Lee, Mi-Gi;Bin, Bum-Ho;Kwak, Byeong-Mun
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.48 no.3
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    • pp.189-196
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    • 2022
  • Plasma refers to an ionized gas that is often referred to as "the fourth phase of matter", following solid, liquid, and gas. Plasma has traditionally been utilized for industrial applications such as welding and neon signs, but its promise in biomedical fields such as cancer treatment and dermatology has lately been recognized. Indeed, due to its beneficial effects in promoting collagen production, improving skin tone, and eliminating harmful bacteria in the skin, plasma treatment constitutes an important target for dermatological research. In this study, a plasma device for cosmetic manufacturing based on nitrogen, the main component of the atmosphere, was designed and assembled. Moreover, nitric oxide (NO) was selected since is easier to follow and evaluate than other nitrogen plasma active species, and its contents were measured to perform a quantitative and qualitative evaluation of plasma. First, an injection method, using different proximities labeled "sinking" and "non sinking" treatments, was performed to test the most efficient plasma treatment method. As a result, it was observed that the formulation obtained by a non sinking treatment was more effective. Furthermore, toner and ampoule were selected as cosmetics formulations, and the characteristics of the formulation and changes in the injected plasma state were observed. In both formulations, the successful injection of NO plasma was 2 times higher in toner formulation than ampoule formulation, and it gradually decreased with time, having dissipated after a week. It was confirmed that the nitrogen plasma used did not affect the stability of the toner and ampoule formulations at low temperature (4 ℃), room temperature (25 ℃), and high temperature (37 ℃ and 50 ℃) conditions. The results of this study demonstrate the potential of plasma cosmetics and highlight the importance of securing the stability of the injected plasma.

Grapevine Growth and Berry Development under the Agrivoltaic Solar Panels in the Vineyards (영농형 태양광 시설 설치에 따른 포도나무 생육 및 과실 특성 변화 비교)

  • Ahn, Soon Young;Lee, Dan Bi;Lee, Hae In;Myint, Zar Le;Min, Sang Yoon;Kim, Bo Myung;Oh, Wook;Jung, Jae Hak;Yun, Hae Keun
    • Journal of Bio-Environment Control
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    • v.31 no.4
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    • pp.356-365
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    • 2022
  • Agrivoltaic systems, also called solar sharing, stated from an idea that utilizes sunlight above the light saturation point of crops for power generation using solar panels. The agrivoltaic systems are expected to reduce the incident solar radiation, the consequent surface cooling effect, and evapotranspiration, and bring additional income to farms through solar power generation by combining crops with solar photovoltaics. In this study, to evaluate if agrivoltaic systems are suitable for viticulture, we investigated the microclimatic change, the growth of vines and the characteristics of grape grown under solar panels set by planting lines compared with ones in open vineyards. There was high reduction of wind speed during over-wintering season, and low soil temperature under solar panel compared to those in the open field. There was not significant difference in total carbohydrates and bud burst in bearing mother branches between plots. Despite high content of chlorophyll in vines grown under panels, there is no significant difference in shoot growth of vines, berry weight, cluster weight, total soluble solid content and acidity of berries, and anthocyanin content of berry skins in harvested grapes in vineyards under panels and open vineyards. It was observed that harvesting season was delayed by 7-10 days due to late skin coloration in grapes grown in vineyards under panels compared to ones grown in open vineyards. The results from this study would be used as data required in development of viticulture system under panel in the future and further study for evaluating the influence of agrivoltaic system on production of crops including grapes.

A Review on the Recycling of the Concrete Waste Generate from the Decommissioning of Nuclear Power Plants (원전 해체 콘크리트 폐기물의 재활용에 대한 고찰)

  • Jeon, Ji-Hun;Lee, Woo-Chun;Lee, Sang-Woo;Kim, Soon-Oh
    • Economic and Environmental Geology
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    • v.54 no.2
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    • pp.285-297
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    • 2021
  • Globally, nuclear-decommissioning facilities have been increased in number, and thereby hundreds of thousands of wastes, such as concrete, soil, and metal, have been generated. For this reason, there have been numerous efforts and researches on the development of technology for volume reduction and recycling of solid radioactive wastes, and this study reviewed and examined thoroughly such previous studies. The waste concrete powder is rehydrated by other processes such as grinding and sintering, and the processes rendered aluminate (C3A), C4AF, C3S, and ��-C2S, which are the significant compounds controlling the hydration reaction of concrete and the compressive strength of the solidified matrix. The review of the previous studies confirmed that waste concretes could be used as recycling cement, but there remain problems with the decreasing strength of solidified matrix due to mingling with aggregates. There have been further efforts to improve the performance of recycling concrete via mixing with reactive agents using industrial by-products, such as blast furnace slag and fly ash. As a result, the compressive strength of the solidified matrix was proved to be enhanced. On the contrary, there have been few kinds of researches on manufacturing recycled concretes using soil wastes. Illite and zeolite in soil waste show the high adsorption capacity on radioactive nuclides, and they can be recycled as solidification agents. If the soil wastes are recycled as much as possible, the volume of wastes generated from the decommissioning of nuclear power plants (NPPs) is not only significantly reduced, but collateral benefits also are received because radioactive wastes are safely disposed of by solidification agents made from such soil wastes. Thus, it is required to study the production of non-sintered cement using clay minerals in soil wastes. This paper reviewed related domestic and foreign researches to consider the sustainable recycling of concrete waste from NPPs as recycling cement and utilizing clay minerals in soil waste to produce unsintered cement.

Fruit Characteristics and Yield according to the Age of "Cheonhwang" Jujube Trees (Zizyphus jujuba var. inermis) (대추나무 '천황'의 수령에 따른 과실품질과 수확량)

  • Park, Seong-In;Chul-Woo Kim;Yoo, Hui-Won;Lee, Uk;Ahn, Young-Sang
    • Journal of Korean Society of Forest Science
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    • v.111 no.4
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    • pp.548-556
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    • 2022
  • In this study, basic data were obtained to determine the optimal cultivation method to achieve stable fruiting and yield increase in "Cheonhwang" jujube trees. Accordingly, thefructification, fruit characteristics, and yield according to tree age were analyzed. The mean (and range of) tree height, crown area, stem diameter near the root, clear stem length, number of main branches, and distance between main branches were 235.6 (217.4-253.8) cm, 3.5 (3.1-4.1) m2, 5.5 (4.0-7.1) cm, 70.6 (66.2-72.7) cm, 9.7 (8.6-10.5), and 10.4 (7.9-14.2) cm, respectively. Correlationanalysis results indicated that tree age was positively correlated with crown area, stem diameter near the root, and clear stem length but not with the number of main branches. The mean number (and range) of fruit per fruit-bearing mother shoot and tree were 18.3 (16.7-18.3) and 170.7 (157.9-178.3), respectively. Tree age was not significantlycorrelated with fruiting characteristics (i.e., the numbers of fruit-bearing mother shoots per main branch, fruit-bearing shoots per fruit-bearing mother shoot, fruit per fruit-bearing shoot, and fruit per tree). Given that the shape of jujube trees is constantly managed according to the growing area and greenhouse type, the tree growth characteristics were more affected by tree management techniques than by tree age. The mean (and range of) fruit weight, fruit hardness, and soluble solid content were 28.6 (27.7-30.3) g, 29.4 (28.5-30.4) N, and 20.4 (19.3-21.0) °brix, respectively, and these fruit characteristics were not significantly correlated with tree age.The average yield per tree of the 'Cheonhwang' jujube cultivar was 4.9 (4.8-5.0) kg, which was not significantly correlated with tree age.

Effects of Coir Substrate Application and Substrate Volume on the Growth and Yields of Strawberry in a Hydroponically Cultured System (딸기 수경재배에 코이어 배지 적용과 근권부 배지 용량이 생육 및 수확량에 미치는 영향)

  • Hwang, Jeongsu;Yun, Sungwook;Kwon, Jinkyung;Park, Minjung;Lee, Dongsoo;Lee, Heeju;Lee, Siyoung;Lee, Sanggyu;Hong, Youngsin
    • Journal of Bio-Environment Control
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    • v.31 no.3
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    • pp.163-169
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    • 2022
  • This study was conducted to examine an alternative cultivating method that uses coir substrates in a hydroponically cultured system. Three treatment conditions were applied with one-layer substrate (10 cm height) with a coir chip and dust ratio of 5:5 (Treatment A), two-layer coir substrate (20 cm height) with a coir chip and dust ratio of 5:5 (Treatment B), one-layer coir substrate (15 cm height) with a coir chip and dust ratio of 7:3 (Treatment C). The control condition was a plastic container filled with a coir chip and dust ratio of 5:5. Various criteria were measured and compared between the treatments and the control. The yield of strawberry was smaller in the control than in the treatments. No significant difference in growth characteristic was found in the height treatments of the coir substrates. The net photosynthetic rate of the treatments was 14.68-15.76 µmol CO2·m-2·s-1. This does not show a statistically significant difference. The root activity was better in treatment B and C than in treatment A and the control. The length and width of leaves were measured as 4.04-4.13 cm and 3.26-3.34 cm. These results are not statistically significant. The leaf length and width ratio was 1.27 in the control and 1.24 in the treatments. The findings show that no statistically significant benefit was found when utilizing coir substrates with different height treatments in the hydroponic culture system. However, the harvested fruit per plant weights 72.38 g in treatment A and 48.69 g in treatment C. The number of harvested fruit was least in treatment C in which a coir chip and dust ratio of 7:3 was applied. Therefore, further research is needed to examine how the chip and dust ratio in coir substrate affects growth characteristics.

Effects of Drip Irrigation Volumes on Plant Growth and Yield of Tomato Grown in Perlite (펄라이트 재배에서 급액량이 토마토의 생육과 수량에 미치는 영향)

  • Kim, Doo Han;Shawon, Md Rayhan Ahmed;An, Jin Hee;Lee, Hyoun Jin;Lee, Yun-Jae;Kim, Minkyung;Lee, Yong-Beom;Choi, Ki Young
    • Journal of Bio-Environment Control
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    • v.31 no.4
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    • pp.300-310
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    • 2022
  • The objective of this experiment was to investigate the effect of drip irrigation volume on tomatoes (Solanum lycopersicum L.) grown in a greenhouse using perlite medium. Plants were treated by three different irrigation treatment I0, I25, and I50 (where irrigation volume of I25 and I50 was 25% and 50% higher than I0, having limited or no leaching). Growth characteristics of plants, yield and water use efficiency were measured. The result showed that plant height, leaf length and leaf width were lowest in the I0 treated plants. However, these parameters were not statistically significant differences between the plants that were grown in the I25 and I50 treatment. Soluble solids content, acidity and dry matter of 111th, 132nd, and 143rd days harvested tomato were higher in the plants irrigated with lowest volume (I0) than the higher volume (I25 or I50). In addition, water content was lower in the 111th and 132nd days of harvested tomatoes from the I0 treatment. The number of big-size tomatoes (>180 g) was significantly higher in the I25 irrigated plants. There was no significant difference in the total number of harvested fruits among the treatments. The average fruit weight and total yield of harvested tomatoes were lowest in the I0 treated plants. The water consumption of tomato was not significantly different amongst the treatments but water use efficiency was lowest in the I0 treatment. Principal component analysis revealed that total soluble solid and acidity of tomato showed a positive correlation between each other. These results suggest that I25 was the optimum irrigation treatment for tomato based on its measured growth characteristics, yield and water use efficiency.

Analysis of Economic and Environmental Effects of Remanufactured Furniture Through Case Studies (사례분석을 통한 사용 후 가구 재제조의 경제적·환경적 효과 분석)

  • Lee, Jong-Hyo;Kang, Hong-Yoon;Hwang, Yong Woo;Hwang, Hyeon-Jeong
    • Resources Recycling
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    • v.31 no.5
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    • pp.67-76
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    • 2022
  • The furniture industry has a high possibility to create value-added and a high potential to create new occupations due to the characteristics of the industry, which mainly consists of small and medium-sized enterprises (SMEs). However, the used furniture, which has sufficient reuse value, is also crushed and used as solid refuse fuel (SRF) recently. Besides, the number of waste treatment companies continues to decrease, and it occurs congestion of wood waste. As a way to solve the issue, a business model development of remanufacturing used furniture can be suggested as an alternative due to its high circular economic efficiency. Remanufacturing business including furniture industry creates positive effects in various aspects such as economic, environmental and job creation. In other words, remanufacturing is an effective recycling way to reduce input resources and energy in the production process. The results of economic analysis show that the expected annual revenue from the single worker furniture remanufacturing site was 104 million won which is 3.11 times more than the average income of a single-worker household in Korea and its B/C ratio was estimated about 30 which means high business feasibility. Revenue through furniture remanufacturing also showed 320 times higher than that of SRF production from the perspective of weight. In addition, it is shown that the GHGs reduction from the furniture remanufacturing is 2.2 ton CO2-eq. per year, which is similar to the amount of GHGs absorption effect of 937 pine trees or 622 Korean oak trees annually. Thus the results of this study demonstrate that it is important to adopt an appropriate recycling method considering the economic and environmental effects at the end-of-life stage.

A Case Study on Service Philosophy : Assetization on Wisdom of the Founding President of the Republic of Korea (서비스철학 사례 연구: 대한민국 건국대통령 지혜의 자산화)

  • Hyunsoo Kim
    • Journal of Service Research and Studies
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    • v.12 no.2
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    • pp.1-22
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    • 2022
  • This study was conducted based on the service philosophy as a study to capitalize on the wisdom of Korea and Koreans. This is a study that capitalizes on the wisdom of the founding president who led civilization changes in Korea. Wisdom as a visionary leader who played a leading role in Korea's development from a continental civilization to a maritime civilization was derived. We analyzed the wisdom of the founding president, who established the Republic of Korea with a new identity as a liberal democracy and protected it, and laid the foundation for Korea to leap into an advanced country through legal system reform and human resource development. First, we analyzed the essence of politics and presented the essential tragic nature that visionary leaders must bear as politicians. We also analyzes his courage and belief to accept a tragic fate, the insights on the spirit of independence, anti-communism, liberal democracy and the future. We analyze the wisdom as a visionary leader that is consistently revealed through countless choices and decisions to give up. It suggests that such wisdom is essential for the founding and development of a nation, and the wisdom need to be assetized. It needs a solid philosophical foundation to become a useful wisdom asset in the long run. In this study, Syngman Rhee's wisdom was assetized on the basis of the service philosophy. This is because it is wisdom based on the fiercely symmetrical balance principle. Human resource development was a choice that entailed great sacrifice, the market economy was a choice that entailed great sacrifice, and the protection of liberal democracy was also a choice that entailed great sacrifice, so it can be said that it is wisdom that fits the essence of the service philosophy. It is wisdom found only in outstanding visionary leaders. We present the wisdom of building a free democratic country, the wisdom of building a free market economy, and the wisdom of building a talent-oriented nation as assets in terms of national management philosophy, national management owner, time frame, and strategic asset. It also suggests that visionary leaders are continuously required for the long-term sustainable development of mankind and Korean society.