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Reduction of Hexavalent Chromium by Shewanella sp. HN-41 in the Presence of Ferric-Citrate (구연산철 환원 조건하에서 Shewanella sp. HN-41에 의한 6가 크롬의 환원)

  • Hyemin Park;Jin-Hyeob Kwak;Ji-Hoon Lee
    • Korean Journal of Environmental Agriculture
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    • v.42 no.3
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    • pp.253-258
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    • 2023
  • In the environment, chromium often exists in a highly mobile and toxic form of Cr(VI). Therefore, the reduction of Cr(VI) to less toxic Cr(III) is considered an effective remediation strategy for Cr(VI)-contamination. In this study, the biological reduction of hexavalent chromium was examined at the concentrations of 0.01 mM, 0.1 mM, and 1 mM Cr(VI) by the dissimilatory metal-reducing bacterium, Shewanella sp. HN-41 in the presence of ferric-citrate. With the relatively condensed cell densities, the aqueous phase Cr(VI) was reduced at the proportions of 42%, 23%, and 31%, respectively for the 0.01 mM, 0.1 mM, and 1 mM Cr(VI) incubations, while Fe(III)-citrate was reduced at 95%, 88%, and 73%, respectively. Although the strain HN-41 was not considered to reduce Cr(VI) as the sole electron acceptor for anaerobic metabolism in the preliminary experiment, it has been presumed that outer-membrane c-type cytochromes such as MtrC and OmcA reduced Cr(VI) in the presence of ferric-citrate as the electron acceptor. Since this study indicated the potential of relatively high cell density for Cr(VI) reduction, it might propose a bioremediation strategy for Cr(VI) removal from contaminated waters using engineered systems such as bioreactors employing high cell growths.

Effect of Various Forms of Floor System on Performance of Meat-type Duck and Environments of Duck House (오리사 바닥 형태가 육용오리의 생산성 및 사육환경에 미치는 영향)

  • Bang, Han-Tae;Kim, Dong-Woon;Hwangbo, Jong;Na, Jae-Cheon;Kang, Hwan-Ku;Kim, Min-Ji;Mushtaq, M.M.H.;Parvin, R.;Choi, Hee-Chul;Lee, Sang-Bae;Kang, Min;Kim, Ji-Hyuk
    • Korean Journal of Poultry Science
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    • v.40 no.3
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    • pp.253-262
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    • 2013
  • This experiment was conducted to investigate the effects of floor type and heating system on performance, housing environment and health status of ducks reared in three types of duck house (OD : Open floor house-Direct heating system, OF : Open floor house-Floor heating system and LD : Loft type house-Direct heating system). In OF treatment, PVC pipes were installed for heating under concrete floor and covered with litter. In LD treatment, plastic mesh was installed 50 cm above the floor so that duck's droppings can pass through it. Each treatment had four replicates of 25 birds (Cherry Valley duck breed) per pen. There were no significant differences in weight gain and feed intake of ducks for 6 weeks among all treatments. However, feed conversion ratio in LD was significantly higher (p<0.05) than that in OF. No differences were found in carcass charac- teristics, with the exception of abdominal fat weight where OF were higher than the others. Concentrations of $CO_2$ and $NH_3$ gas in OD were higher than those of OF and LD at 3, 4 and 5 weeks. Moisture content in litter of OF was lower than that of OD. In contrast, the amount of dust in the air was higher in OF than in OD. The amount of fuel used for 6 weeks in LD was lower about 21% than that in OD. Some of unusual symptoms were observed in open floor house and loft type house, such as lying, spraddle legged, twisted ankle and legs, wounded sole, or etc. No components of leukocyte and erythrocyte of blood were significantly different among all treatments. The results of this experiment showed that OF and LD systems had no positive effects on performance of meat type commercial duck. However, there were some positive effects of certain house type for the improvement of environmental condition in duck house for hygienic production. In the future, more research on the effect of various facilities and systems for duck house is needed.

The Research on the Development Procedure and Current Problems of the Korean Abalone Industry (전복 양식업의 발전과정과 당면과제 연구)

  • Ock, Young-Soo
    • The Journal of Fisheries Business Administration
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    • v.44 no.3
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    • pp.15-28
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    • 2013
  • Abalone aquaculture has developed very rapidly in Korea. Until the mid 1990s it has annually produced about 100 tons. Since then the yield has increased to about 9,000 tons in 2012. The amount accounts for 20% of the global abalone yield. About 86% of produced abalone is consumed domestically and the rest is exported. 100 tons for export seemed as an unattainable goal back in 2003. However, the export rose up to 1,333 tons in 2012. Despite its rapid growth, Korean abalone industry is faced with some problems. The first is the slowdown of yield increase rates. Abalone production increased by 50~60% until the mid 2000. However, the rate continued to drop to below 10%. Reasons behind the slow increase are deteriorating aquaculture grounds and worsening market problems. Constant aquaculture aggravated productivity and overcrowded facilities at a limited space made matters worse. Moreover, abalone export has stalled and so did domestic consumption. In the meantime, rising mortality of young abalone has lowered productivity at abalone breeding places. The mortality rates of abalone remained below 5% in the early 2000s but rose to 30~40% these days. This translates into rising abalone prices. The market problems imply stagnant or shrinking export as well as domestic consumption. The export increase rates took a nosedive from 200 to below 50 between the early 2000s and the late 2000s. Moreover, the increase rates of domestic consumption have become remarkably sluggish. According to, it stood at 50~60% in the mid 2000s but continued to decrease after 2008. These problems, in turn, affected the size of abalone. The usual abalone size for market was 10~12 shells per kg, but recently the size became smaller and smaller to 15~16 shells per kg. The change of size implies shift in consumption patterns: Consumers not only eat live abalone but also they cook soup with it. The size of abalone for uncooked dish is usually very big, like 10~12 shells per kg. In contrast, smaller abalone, such as 20~25 shells per kg, are used for making soup. Increasing use of smaller abalone leads to lower income of abalone aquaculture households. This is partly because that the size determines the price and the price gap between big abalones and smaller ones is extreme in Korea. For the sustainable growth of Korean abalone industry, we need to come up with strategies. First, a reasonable production system needs to be in place, especially for better management of abalone aquaculture grounds. Management of abalone licenses is also necessary because local governments issue relevant licenses as well as supervising abalone grounds. Second, abalone export destination need to be diversified. Japan, the major importer of Korean abalone, takes up a lion's share of export, at 95%. Third, new consumption style of abalone needs to be developed. Abalone used to be consumed as 'raw type' or Sashimi in Korea. This sole type of consumption hampers the growth of abalone market. Moreover, more strategies are needed to encourage and distribute home cooking of abalone rather than eating-out at restaurants. Last but not least, distribution system should be improved for better delivery of live abalone.

Taxonomic Studies on the Cellulolytic Bacterium Cellulomonas uda CS 1-1 (섬유소 분해균 Cellulomonas uda CS 1-1의 분류학적 연구)

  • Kim, Mi-Seog;Yoon, Min-Ho;Choi, Woo-Young
    • Korean Journal of Agricultural Science
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    • v.34 no.2
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    • pp.99-109
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    • 2007
  • Cellulomanas sp. CS 1-1 was studied for its morphological, physiological and biochemical characteristics, together with DNA homology and fatty acid pattern to elucidate its taxonomical position in the species level. Colony morphology of CS1-1 exhibited circular form, opaque, convex, entire edge and pale yellow. Cells were of rod with the size of $0.3{\sim}0.5{\times}0.8{\sim}1.2{\mu}m$, while coryneforms were formed at the early stage of culture. D-ribose, raffinose, rhamnose, acetate, propionate, L-lactate, D-gluconate, aspartate and proline were not utilized as a sole source of carbon, whereas saccharose, arabinose, and amlyose were utilized. Biochemical characteristics of CS1-1 were Gram positive, catalase positive, oxidase negative, nonmotile, facultative anaerobic, mesophilic and G+C content of 74.7 mol %. The major fatty acid and menaquinone were 12-methyltetradecanoic acid(anteiso-$C_{15:1}$) and MK-$9(H_4)$, respectively. These results were correspondent with the characteristics reported for member of the genus Cellulomonas. The strain CS 1-1 exhibited a high level of DNA homology as 70% with C. uda ATCC491, compared to those of 54~59% with C. fimi ATCC 15724, 46~48% with C. biazotea, C. gelida and C. bibula. Finally, strain CS1-1 could be classified as a novel species belongs to C. uda.

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Study on Achievement of Nursing Students-Relationship between Psychological Test Characteristics and Academic Achievement of Nursing Students in a Baccalaureate Program- (간호학생의 학업성취에 관한 연구 -대학 간호학생의 심리적 제특성과 학업성취와의 관계-)

  • 이은옥;이미라
    • Journal of Korean Academy of Nursing
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    • v.3 no.1
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    • pp.53-66
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    • 1972
  • There is an urgent need to improve the tool predicting success or failure of academic achievement of nursing in Korea so as to identify as early as possible those students who should receive special instruction and to improve screening procedures for admission of nursing. The main purpose of this study is to identify the correlation between the grade point averages of courses learned and their psychological test characteristics in a baccalaureate nursing program. All 240 students, except freshmen, enrolled in Nursing Department of Seoul National University in the spring semester, 1972, participated in this study. All of the subjects completed the psychometric tests such as interest test, personality test and test of self-concept. Total grade point averages, grade point averages of general education subjects, of supporting science courses and of professional education subjects were used as performance criteria of the students. Through the calculation of product-moment correlation coefficients between the test scores and four grade point averages of each class and of total subjects, the following findings and recommendations were obtained. 1. There was so much variation in characteristics of interest test correlated with academic achievement of nursing students in each class. 2. Since the school objectives, curriculum and teaching strategies may affect predictive efficiency of characteristics of students'interest test, interest test must-be utilized in a homogeneous group in order to predict school achievement. 3. Characteristics of interest test positively correlated at significant level with total grade point averages of all subjects were scientific interest-biological, scientific interest-physical, and humanitarian interest. Scientific interest-physics: was the only characteristic positively correlated at significant level with total grade point averages and grade point averages of professional courses. 4. There were various patterns in characteristics of personality test correlated with school achievement of nursing students by class pattern and personality maturation as they progress toward higher classes. 5. A characteristic of personality test, responsibility, is in high positive correlation with academic achievement in the upper division of classes. 6. Responsibility was the sole personality factor positively correlated at significant level with total grade point averages and grade point averages of nursing courses in the total number of students. 7. There were very different correlation coefficients between characteristics of self-concept test and academic achievement according to the type of each class and type of courses they learned. 8. Characteristics of self-concept test positively correlated at significant level with total grade point averages and grade point averages of nursing courses of all students were physical self and row variability. Those who have positive concept on their own physical status and who are deficient in self-concept were higher in total grade point averages and grade point averages of professional courses than other students. 9. Scores of professional courses offered in freshmen and sophomore classes were in positive correlation with limited number of characteristics of psychological tests. In pursuit of a tool predicting successful academic achievement of nursing students, their G.P.A. during the junior and senior year of nursing will serve as the more reasonable criteria. 10. Junior students of this school were in higher positive correlation with many psychological factors than other classes.

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Diversity of VC and incidence of hypovirulence-associated ds-RNAs in the chestnut blight fungus Cryphonectria parasitica in Korea

  • Byeongjin Cha;Jinyoung Lim;Ju, Young-Jik;Kim, Dae-Hyuk
    • Proceedings of the Korean Society of Plant Pathology Conference
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    • 2003.10a
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    • pp.23-23
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    • 2003
  • Chestnut blight, caused by Cryphonectria parasitica, is the most destructive disease of American and European chestnut trees. A total of 672 C prasitica was isolated from blight lesion on chestnut twigs, which were collected from major chestnut plantations all over Korea in 1999. Isolation rates were over 30% in Kyunggj-, Kyongnam-, and Chonnam-do. The highest isolation rate was 37.4% and recorded in Kyongnam-do. On the other hand, Chonbuk-do had the lowest isolation rate as 13.5%. In grouping of C parasitica by colony shape and color, yellow colony with irregular margin were the most dominant colony type with a frequency of 65.2%. When the 672 isolates were inoculated on the chestnut twigs, 380 isolates (56.5%) caused lesions larger than the standard virulent isolate EP155-2, while 158 isolates (23.4%) caused smaller lesions than the standard hypovirulent isolate UEP-1. In Bavendamm test that determines phenol oxidase activity, 97.1% of all the isolates resulted the same or darker discoloration than EP155-2, and only 12.2% resulted the same or lighter discoloration than UEP-1. In the vegetative compatibility (VC) tests, total 670 isolates were divided into 121 VC groups (VCGs). Kyongnam-, Chonnam-, and Chungnam-do, the three principal chestnut plantation area, had 49, 33, and 27 VCGs, respectively. Among the VCGs, the biggest VCG, KR-VC104, was composed of 164 isolates and the second biggest VCG had 62 isolates. But, 64 of 121 VCGs consisted of sole member. More than 65.8% of KR-VC104, was isolated from the three provinces, Kyongnam-, Kangwon-, and Chungbuk-do. In KR-VC104, 62.8%, 59.1%, and 85.9% of the isolates looked like virulent in colony type, pathogenicity test, and Bavendamm test. In ds-RNA detection tests using cellulose chromatography, 77 of total 650 isolates were ds-RNA positive and detected ds-RNA segments were approximately 12kb, 3kb, 2.7kb, 2kb, and 1.8kb in size. Among the 77 isolates, 46 isolates had 12kb and 25 isolates had 12kb and 2.7kb. Other 6 Isolates had small ds-RNA segments. Kyongnam-, Chonnam-, and Chungnam-do had 43, 16, and 5 ds-RNA positive isolates, respectively. Among the 121 VCGs, only 29 VCGs had ds-RNA positive isolates.(중략)

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Development of Shoes' Easy-Order Prototype According to Foot Types of Juveniles In On-Line 3D Virtual Reality (청소년의 발의 형태 분류에 따른 On-line 3D 가상현실에서의 신발류 이지오더 Prototype 개발)

  • Choi, Sung-Won;Lim, Ji-Young
    • The Journal of the Korea Contents Association
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    • v.6 no.11
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    • pp.182-189
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    • 2006
  • Consumers can choose suitable shoes for their feet in off-line shopping. However, in on-line shopping, because they can not wear shoes, compare to the off-line shopping, there are many problems in internet shopping. And the solution of user-oriented internet shopping is development of new type of prototype which is accessible to user and to offer visual information through 3D-virtual reality. We made this Prototype that the consumer can measure their own shoes size. And the consumer can print out their foot size in the internet database and measure their own foot size and type. And we maximized the visual experience though it is indirect and then we wanted to overcome a emotional experience in the real world. we visualized first, 'the softness' of shoes meterial, second 'the drainage' in the realtion of shoes meterial and water, third 'the close adhesion' in the realtion of shoes and consumer's their own foot and last 'the elasticity' in the relation of the shoes outer sole and surface. The result of this research can solve the problem in the existing on-line shoes' shop and it will become an alternative plan. And this prototype just will not become the localization at the on-line shoes' shop. In true sense, it will be an important example in the whole internet industry.

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Ramlibacter terrae sp. nov. and Ramlibacter montanisoli sp. nov., Isolated from Soil

  • Khan, Shehzad Abid;Kim, Hyung Min;Baek, Ju Hye;Jung, Hye Su;Jeon, Che Ok
    • Journal of Microbiology and Biotechnology
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    • v.31 no.9
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    • pp.1210-1217
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    • 2021
  • Two gram-negative, catalase-positive, strictly aerobic, and white colony-forming bacteria, strains H242T and B156T, were isolated from soil in South Korea. Cells of strain H242T were oxidase-positive and non-motile short rods, while those of strain B156T were oxidase-negative and long non-motile rods. Ubiquinone-8 was identified as the sole isoprenoid quinone in both strains. C16:0, cyclo-C17:0, andsummed feature 3 (C16:1 ω7c and/or C16:1 ω6c) and phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol were identified in both strains as the major cellular fatty acids and polar lipids, respectively. The DNA G+C contents of strains H242T and B156T were 69.4 mol% and 69.3 mol%, respectively. Phylogenetic analyses based on 16S rRNA and 92 concatenated core gene sequences revealed that strains H242T and B156T formed distinct phylogenic lineages from other Ramlibacter type strains. The DNA-DNA hybridization (DDH) value between strains H242T and B156T was 24.6%. Strains H242T and B156T were most closely related to Ramlibacter ginsenosidimutans BXN5-27T and Ramlibacter monticola G-3-2T with 98.4% and 98.6% 16S rRNA gene sequence similarities, respectively. Digital DDH values between strain H242T and R. ginsenosidimutans and between strain B156T and R. monticola were 23.5% and 26.1%, respectively. Phenotypic, chemotaxonomic, and molecular analyses indicated that strains H242T and B156T represent two novel species of the genus Ramlibacter, for which the names Ramlibacter terrae sp. nov. and Ramlibacter montanisoli sp. nov., respectively, are proposed. The type strains of R. terrae and R. montanisoli are H242T (=KACC 21667T=JCM 33922T) and B156T (=KACC 21665T=JCM 33920T), respectively.

Retrospective study of the $Implantium^{(R)}$ implant with a SLA surface and internal connection with microthreads (SLA 표면 처리와 미세나사선을 가진 내측 연결형의 국산 임플란트에 대한 후향적 연구)

  • Doh, Re-Mee;Moon, Hong-Suk;Shim, Jun-Sung;Lee, Keun-Woo
    • The Journal of Korean Academy of Prosthodontics
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    • v.47 no.2
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    • pp.136-147
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    • 2009
  • Statement of problem: Since the introduction of the concept of osseointegration in dental implants, high long-term success rates have been achieved. Though the use of dental implants have increased dramatically, there are few studies on domestic implants with clinical and objective long-term data. Purpose: The aim of this retrospective study was to provide long-term data on the $Implantium^{(R)}$ implant, which features a sandblasted and acid-etched surface and internal connection with microthreads. Material and methods: 106 $Implantium^{(R)}$ implants placed in 38 patients at Yonsei University Hospital were examined to determine the effect of various factors on implant success and marginal bone loss, through clinical and radiographic results during a 6 to 30 month period. Results: 1. Out of a total of 106 implants placed in 38 patients, one fixture was lost, resulting in a 99.1% cumulative survival rate. 2. Among the 96 implants which were observed throughout the study period, the survival rates were 97.0% in the maxilla and 100% in the mandible. The survival rate in the posterior regions was 98.9% and 100% in the anterior regions. 3. The mean bone loss during the first year after prosthesis placement was 0.17 mm, while the mean annual bone loss after the first year was 0.04 mm, which was statistically less than during the first year(P<.05). 4. There was no significant difference in marginal bone loss according to age during the first year(P>.05), but after the first year, the mean annual bone loss in patients above 50 years was significantly greater(P<.05) compared with patients under 50 years. 5. No significant difference in marginal bone loss was found according to the following factors: gender, jaw, location in the arch, type of implant(submerged or non-submerged), presence of bone grafts, type of prostheses, and type of opposing dentition(P<.05). Conclusion: Based on these results, the sole factor influencing marginal bone loss was age, while factors such as gender, jaw, location in the arch, type of implant, presence of bone grafts, type of prostheses and type of opposing dentition had no significant effect on bone loss. In the present study, the success rate of the $Implantium^{(R)}$ implant with a SLA surface and internal connection with microthreads was satisfactory up to a maximum 30 month period, and the marginal bone loss was in accord with the success criteria of dental implants.

Study on Influencing Factors of Traffic Accidents in Urban Tunnel Using Quantification Theory (In Busan Metropolitan City) (수량화 이론을 이용한 도시부 터널 내 교통사고 영향요인에 관한 연구 - 부산광역시를 중심으로 -)

  • Lim, Chang Sik;Choi, Yang Won
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.35 no.1
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    • pp.173-185
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    • 2015
  • This study aims to investigate the characteristics and types of car accidents and establish a prediction model by analyzing 456 car accidents having occurred in the 11 tunnels in Busan, through statistical analysis techniques. The results of this study can be summarized as below. As a result of analyzing the characteristics of car accidents, it was found that 64.9% of all the car accidents took place in the tunnels between 08:00 and 18:00, which was higher than 45.8 to 46.1% of the car accidents in common roads. As a result of analyzing the types of car accidents, the car-to-car accident type was the majority, and the sole-car accident type in the tunnels was relatively high, compared to that in common roads. Besides, people at the age between 21 and 40 were most involved in car accidents, and in the vehicle type of the first party to car accidents, trucks showed a high proportion, and in the cloud cover, rainy days or cloudy days showed a high proportion unlike clear days. As a result of analyzing the principal components of car accident influence factors, it was found that the first principal components were road, tunnel structure and traffic flow-related factors, the second principal components lighting facility and road structure-related factors, the third principal factors stand-by and lighting facility-related factors, the fourth principal components human and time series-related factors, the fifth principal components human-related factors, the sixth principal components vehicle and traffic flow-related factors, and the seventh principal components meteorological factors. As a result of classifying car accident spots, there were 5 optimized groups classified, and as a result of analyzing each group based on Quantification Theory Type I, it was found that the first group showed low explanation power for the prediction model, while the fourth group showed a middle explanation power and the second, third and fifth groups showed high explanation power for the prediction model. Out of all the items(principal components) over 0.2(a weak correlation) in the partial correlation coefficient absolute value of the prediction model, this study analyzed variables including road environment variables. As a result, main examination items were summarized as proper traffic flow processing, cross-section composition(the width of a road), tunnel structure(the length of a tunnel), the lineal of a road, ventilation facilities and lighting facilities.