• Korean Journal of Microbiology
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• v.24 no.4
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• pp.394-399
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• 1986

From the lysates of the 7 selected strains of Pseulomonas utilizing 2-methyl-4-chlorophenoxyactate as a sole source of carbon and energy, several MCPA plasmids, which encodes genes for the degradation of 2-methyl-4-chlorophenoxyacetate, were isolated, and measured their molecular weight as well as genetic characters such as resistance to antibiotics and degradative ability of other chlorinated herbicides. Transmissibility of the MCPA plasmids, pKU1, pKU15, and pKU17 was tested by conjugation or transformation and the restriction pattern of pKU15 for Pvu II, Hind III, EcoR I, Xho I, Bgl II, and Ava II was analyzed.

• Korean Journal of Microbiology
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• v.52 no.4
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• pp.503-504
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• 2016

Ochrobactrum anthropi AM3 was isolated for the ability to utilize lignin as a sole carbon and energy source from compost in South Korea. Here we report the 5.11 Mb draft genome of strain AM3 with a G+C content of 56.2%, which is helpful for understanding the genetic diversity among Ochrobactrum spp. and the mechanism of lignin degradation.

• Journal of Environmental Health Sciences
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• v.16 no.2
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• pp.75-82
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• 1990

Carbaryl was degradaded to monomethylamine(MA) by bacterium. which isolated from lake sediment. Its carbaryl degradation was maximized when grown on mineral salt medium conditioning 20 $\mu$M of carbaryl as a sole carbon source at 26${\circ}$C and initial pH 7.0-9.0 Its degradation ability was minimized at initial pH 3.0 and 5.0 The percent conversions { (moles of MA in excess of control / mole of carbaryl added) $\times$ 100} for 10. 20. 40 and 80 $\mu$M of carbaryl were 18.6. 16.1. 18.1 and 11.6 respectively. It suggests that increasing amount of carbaryl added above 80 $\mu$M. the percent conversion should be decreased. The MA production by the bacterium was lineary related to the cultural time. After 7days culture. its percent conversion was 46.2. and this result suggests that a half amount of carbaryl would be hydrolyzed to MA by the bacterium within 8 days.

• Journal of Environmental Science International
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• v.19 no.11
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• pp.1391-1396
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• 2010

Three biphenyl-degrading microorganisms were isolated from polluted soil samples in Sasang-gu, Busan. Among them, isolate DS-94 showing the strong degrading activity was selected. The morphological, physiological, and biochemical characteristics of DS-94 were investigated by API 20NE and other tests. This bacterium was identified as the genus Pseudomonas by 16S rDNA sequencing and designated as Pseudomonas sp. DS-94. The optimum temperature and pH for the growth of Pseudomonas sp. DS-94 were $25^{\circ}C$ and pH 7.0, respectively. This isolate could utilize biphenyl as sole source of carbon and energy. Biphenyl-degrading efficiency of this isolate was measured by HPLC analysis. As a result of biological biphenyl-degradation at high biphenyl concentration (500 mg/L), biphenyl-removal efficiency by this isolate was 73.5% for 7 days.

• 한국생물공학회:학술대회논문집
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• 2000.11a
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• pp.291-294
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• 2000

The production of polyhydroxybutrate(PHB) by Alcaligenes eutrophus NCIB 11599 was studied in a synthetic medium from whey as a sole carbon source. Especially pH-effect was treated and compared in this study. At the end of fermentation (A) unadjusted to pH, the dry cell weight, PHB concentration, and PHB conversion rate were 10.3g/L, 3.1g/L, and 30%, respectively. At the end of fermentation (B) adjusted to $pH(7.0{\sim}7.5)$, the dry cell weight, PHB concentration, and PHB conversion rate were 12.5g/L, 4.8g/L, and 41%, respectively. PHB conversion rate was about 10% higher on the fermentation (B) than on the fermentation (A).

• 한국생물공학회:학술대회논문집
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• 2005.10a
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• pp.343-346
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• 2005

The isolated strain, SC2-1 was Gram-positive, catalase positive, facultatively anaerobic, oxidase negative, motile and small rods. The strain utilized sucrose, dextrose, fructose, mannitol and maltose as a sole carbon and energy source and sodium chloride required for the bacteria growth. The radical scavenging activity of the culture supernatants was determined by DPPH (1,1-diphenyl-2-picrylhydrazyl) method. This bacterium was identified based on cellular fatty acids analysis and 16S rDNA sequencing then named Exiguobacterium sp. SC2-1. The optimum culture conditions for production of antioxidant were $25^{\circ}C,$ pH 7.8 and NaCl concentration were 4%. The modified optimal medium compositions were maltose 2.5% (w/v), yeast extract 1.5% (w/v) and $KH_2PO_4$ 0.05% (w/v). Free radical scavenging activity of under optimal culture conditions were 93%.

• Journal of Environmental Health Sciences
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• v.21 no.4
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• pp.44-48
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• 1995

26 bacterial strains capable of growing on Terephthalic acid (TPA) in minimal medium were isolated from soil and wastewater by selective enrichment culture, and among them, one isolate which was the best in the cell growth and TPA degradation was selected and identified as Pseudomonas sp. T-1 by its characteristics. Cell growth almost revealed a stationary phase at 24 hrs after cultivation. Cell growth dramatically increased in a minimal medium containing 0.1% of TPA as a sole carbon source and TPA was not detected any more at 80 hrs after cultivation. Therefore, it is suggested that Pseudomonas sp. T-1 could be effectively used for the biological treatment of wastewater containing TPA.

• Journal of Environmental Science International
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• v.6 no.4
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• pp.379-384
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• 1997

The biodegradable characteristics of poly-$\beta$-hydroxybutyrate(PHB) film by fun맥 and soil burial are Investigated. As the results of the American Standards for Testing and Materials(ASTM) method, the you of Aspergillus niger was apparent on the PHB containing plate. This suggests that PHB was utilized as the sole carbon source by Aspergillus niger and ASTM method may have applications as measuring means of biome gradability of polyhydroxyalkanoic acid(PHA). PHB film was studied by monitoring the time-dependant changes in weight loss of PHB film under 30% and relative humidity 80 % during pot-test. As the results of pot-test, PHB film was decomposed about 87 % in 30 days by soul microorganisms. PHB film was more slowly degraded than PHB/HV film.

• Microbiology and Biotechnology Letters
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• v.22 no.3
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• pp.316-321
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• 1994

Several strains capable of degrading PEGs(Polyethylene Glycols) were isolated and investigated for their biodegradation ability of PEGs. Microorganisms screened for the biodegrada- tion studies were those grown on the PEG used as a sole carbon and energy source. It was known that the number of microorganisms decreased when grown on the high molecular weight of PEG. A biodegradation of PEG was investigated with such microorganisms in the reactor and resulted in the decrease in PEG concentration meaning that PEG was degraded in the reactor. This microorganism was identified as Flavobacterium sp. The biodegradability was found to be about 18.8% for PEG-8000 and 25.4% for PEG-10,000, respectively. For the manufacture of biodeg- radable PEG film, EMAA/PEG and EAA/PEG blending ability was investigated with IR spectrum and showed that it was possible to produce blending film.

• Journal of Microbiology and Biotechnology
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• v.13 no.2
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• pp.309-312
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• 2003

A bacterial strain NB01, isolated from wastewater, was found to utilize nitrobenzene (NB) as the sole source of nitrogen, carbon, and energy. The strain was classified as a member of a high G+C Gram-positive group and identified as Mycobacterium chelonae based on an analysis of its 16S rRNA gene sequence. The strain grew on NB with a concomitant release of about 63% of the total available nitrogen as ammonia, suggesting a reductive degradation mechanism. The optimal pH and temperature for degradation were PH 7.0-8.0 and $30^{\circ}C$, respectively. The cell growth was retarded at NB concentrations above 1.8 mM. The degradation of NB followed Michaelis-Menten kinetics within the tolerance range, and the $K_m$ and maximum specific removal rate for NB were 0.33 mM and $11.04\;h^{-1}$, respectively.