• Journal of the Korean Society of Food Culture
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• v.24 no.1
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• pp.84-89
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• 2009

This paper was conducted to evaluate aglycones and carbohydrates produced by acid hydrolysis of three potato glycoalkaloids [(PGA); ${\alpha}$-chaconine, ${\alpha}$-solanine, and demissine] in potatoes. Standard solanidine and demissidine were dissolved in 1N HCl and then heated at $100^{\circ}C$ for 10-120 min. Solanidine was rapidly decomposed during acid hydrolysis and one peak that was identified as solantherene ($M^+$=379) by GC-MS was detected. The transformation solanidine to solanthrene was approximately 50% complete after 10 min, approximately 90% complete after 60 min and 100% complete after 120 min. Demissidine was hydrolyzed using the same method that was used to hydrolyze the solanidine. However, demissidine produced only one peak upon GC-MS ($M^+$=399) analysis and was found to be very stable at increased temperatures. Acidy hydrolysis of ${\alpha}$-chaconine, ${\alpha}$-solanine and demissine resulted in the decomposition of ${\alpha}$-chaconine and ${\alpha}$-solanine to solanidine and solanthrene, respectively. Therefore, this hydrolysis method should not be utilized to produce PGA combining with solanidine as aglycone. The individual carbohydrates produced by the two PGAs by hydrolysis were very stable at increased temperatures; therefore, it was possible to quantify these PGAs based on calculation of the individual carbohydrate content. Conversely, because demissidine produced by the hydrolysis of demissine was extremely stable at increased temperatures, it was possible to quantify the PGA based on the aglycone produced by hydrolysis.

• Nuclear Engineering and Technology
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• v.2 no.3
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• pp.179-183
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• 1970

(4-$^{14}$ C) Cholesterol was administered to the leaves of Solanum andigena during photoperiodic induction. Radioactive products converted from cholesterol were studied by thin-layer chromatography and gas-chromatography. The major products from cholesterol were shown to be esterified cholesterol in lipid and solanine in the aqueous ethanolic phase in SD-and LD-leaves. The radioactive solanidine was isolated by chromatography and crystallized to constant specific activity. Short-day condition did not stimulate the conversion of cholesterol into solanine in the leaves, but both groups of leaves converted cholesterol into solanine at the about same rate. Incorporation of radioactivity into aqueous ethanolic phase of tubers and stolons, containing storied glycoalkaloid, was very much higher than that into lipid phase, contrary to those in the leaves and the stems.