• Proceedings of the Korean Society of Agricultural Engineers Conference
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• 한국농공학회 2001년도 학술발표회 발표논문집
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• pp.366-369
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• 2001

This study was performed to investigate the degree of pollution of sediments of stream and reservoir in rural area. A series of site investigations were carried out for Kyongki-do area and chemical analysis were performed for sediment samples. It was found that some samples were heavily polluted with phenol and TPH and gave off a malodor. Soil Pollution Scores(SPSs) was determined for sediment samples. Some samples were classified to Soil Pollution Class(SPC) 2 and 3. For recycling and disposal of dredged sediments from stream and reservoirs, these polluted sediments should be carefully considered. A further study on the criterion of recycling/disposal of sediments and development of new Soil Pollution Index compatible for stream sediments is necessary in future.

• Journal of Korea Soil Environment Society
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• 제3권2호
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• pp.31-39
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• 1998

A total of 112 soil samples collected from polluted and unpolluted areas in Korea were investigated for physical properities (such as soil moisture, organic matter and soil pH) and biological properties (such as microbial numbers). The results of organic matter and soil pH showed a great variation(p=0.01) in the four areas, whereas soil moisture and organic matter were similar among three plant vegetations. There was a significant relationship(p=0.01 or 0.05) between soil pH and microbial number These results imply some variations in soil environment and may lead to unfavorable changes of plant vegetation in soil. Presumably, the above results appear to be resulted from soil acidification caused by an acidic rain.

• Journal of Soil and Groundwater Environment
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• 제18권1호
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• pp.112-122
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• 2013

Current status of soil contamination with fluorine and its source were investigated. The basic principles and procedures of various techniques for the analysis of fluorine contents in soil and solid phase samples were summarized in this review. Analysis of fluorine in solid matrices can be achieved by two types of techniques: (i) UV/Vis spectrophotometer or ion selective electrode (ISE) analysis after performing appropriate extraction steps and (ii) direct solid analysis. As the former cases, the standard method of Korean ministry of environment, alkali fusion-ISE method, pyrohydrolysis, oxygen bomb combustion, aqua regia digestion-automatic analysis, and sequential extraction-ISE method were introduced. In addition, direct analysis methods (i.e., X-ray fluorescence spectrometry and proton induced gamma-ray emission spectrometry) and atomic spectrometry combining with the equipment for introducing solid phase sample were also reviewed. Fluorine analysis techniques can be reasonably selected through site-specific information such as matrix condition, contamination level, the amount of samples and the principles of various methods for the analysis of fluorine presented in this review.

• Research in Plant Disease
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• 제10권4호
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• pp.310-312
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• 2004

Pseudomonas syringae pv. actinidiae is the causative agent of bacterial canker in kiwifruit. A nested PCR detection method that uses primers designed from the cfl gene, involved in production of the phytotoxin coronatine, was applied on soil samples. These primers yielded 665 and 310-bp fragments in consecutive PCR amplification step with DNA from soil inoculated with Korean strain of P. syringae pv. actinidiae. This system was applied to survey soil samples from a kiwifruit orchard destroyed by bacterial canker. A specific 310-bp PCR product was obtained from all six samples of soil tested.

• Korean Journal of Environmental Agriculture
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• 제23권4호
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• pp.251-257
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• 2004

Analytical methods were developed to determine cyclosulfamuron residues in soil, water, rice grain and straw using high-performance liquid chromatography (HPLC) with ultraviolet absorption detection. In these methods, cyclosulfamuron was extracted with aqueous $Na_2HPO_4$/acetone and acetone/methanol mixture from soil and rice samples respectively. Liquid-liquid partition coupled with ion-associated technique, Florisil column chromatography, and solid-phase extraction (SPE) were used to separate cyclosulfamuron from interfering co-extractives prior to HPLC analysis. For water sample, the residue was enriched in $C_{18}$-SPE cartridge, cleaned up in situ, and directly subjected to HPLC. Reverse-phase HPLC under ion-suppression was successfully applied to determine cyclo-sulfamuron in sample extracts with the detection at its ${\lambda}_{max}$ (254 nm). Recoveries from fortified samples averaged $87.8{\pm}7.1%$ (n=12), $97.3{\pm}7.2%$ (n=12), $90.8{\pm}6.6%$ (n=6), and $78.5{\pm}6.7%$ (n=6) for soil, water, rice grain and straw, respectively. Detection limits of the methods were 0.004 mg/kg, 0.001 mg/L, 0.01 mg/kg and 0.02 mg/kg for soil, water, rice grain and straw samples, respectively.

• Journal of Life Science
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• 제31권4호
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• pp.377-384
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• 2021

Researching the soil fungal community is important to understand the interaction between fungi and living plants. However, too few studies have examined the soil fungal community and their interactions with plants. Rhododendron mucronulatum, commonly known as Korean rosebay, is an important forest resource that has aesthetic, ecological, and potential pharmacological values. We used a pyrosequencing method to analyze the characteristics of fungal communities from R. mucronulatum soil samples from Biseul mountain county park, which is one of the famous places for large R. mucronulatum colonies in South Korea. We collected soil core samples in February and August at three sites in the Biseul Mountain County Park, taking into consideration the regional and seasonal conditions. We obtained 454,157 validated reads after pyrosequencing all six samples. The fungal communities from the first observation spot in August had the richest species diversity among the samples. Basidiomycota, Ascomycota, and Mortierellomycota were major phyla in the samples. Agaricales_f, Mortierellaceae, and Clavariaceae were major families in the samples. The genus Mortierella was the most dominant in all six samples. Overall, 19 genera could be associated with R. mucronulatum. Sample 1 had 109 genera in sample 1, sample 2 had 111 genera, and sample 3 had 112 genera that were uniquely identified. The samples collected in August had 28 identified genera, that existed only in summer samples, indicating a weather effect. This study can be used as basic research to understand the relationship between soil fungi and plants.

• Journal of Soil and Groundwater Environment
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• 제21권6호
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• pp.169-178
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• 2016

The Standards, Measurement and Testing Programme (SM&T-formerly BCR) extraction procedure was applied to fractionate Cr, Cu, Ni, Pb and Zn in 23 top soil samples into: (i) exchangeable phase; (ii) reducible phase; (iii) oxidisable(sulfides and organics bound) phase; and (iv) residual phase. Fractions of Cr and Ni were in the order of residual > oxidisable > reducible > exchangeable phase. The oxidisable phase was identified as dominant for Cu and Pb. Zn had the highest ratio of exchangeable phase in comparision to the other metals. The bioavailability and mobility were assessed to be the greatest for Zn, followed by a decreasing order of Pb, Cu, Ni and Cr. All metal average concentrations in topsoil samples was higher in industrial sites than in agricultural sites. Our results revealed higher concentrations in topsoil samples (0~15 cm) than in sub soils (15~30 cm, 30~60 cm) for most metals at six sites (No. 5, 6, 17, 19, 20, 23). The fractions of exchangeable, reducible ad oxidisable phases showed relatively high correlation with soil pH, Fe/Mn oxide concentrations and organic matter contents, respectively.

• Proceedings of the Korean Society of Toxicology Conference
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• 한국독성학회 2005년도 춘계 국제심포지엄 및 학술대회
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• pp.179-179
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• 2005

• Environmental Engineering Research
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• 제24권2호
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• pp.289-297
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• 2019

Indiscriminate disposal of waste and citation of open dumpsites are some of the key factors affecting the various soil geotechnical properties. Atterberg limit and consolidation tests were conducted to determine the effects of two open waste dumpsites (Uselu Market and New Benin) on geotechnical properties of their underlying soils. Soil sample collected from Uselu Market dumpsite in Benin City metropolis showed slightly lower hydraulic conductivity (K) of $1.0{\times}10^{-6}$ with plasticity index of 18.53% compared to sample collected 1.6 m from the same dumpsite which had high K value of $2.42{\times}10^{-3}$ with plasticity index of 6.9%. Soil sample collected from New Benin dumpsite in Benin City metropolis showed slightly lower K of $1.45{\times}10^{-6}$ with plasticity index of 13.8% than sample collected 1.6 m from the same dumpsite which had high K value of $2.14{\times}10^{-2}$ with plasticity index of 6.0%. X-ray florescent analyser (X-MET 7000) and direct soil pH meter were used to determine the composition of the aforementioned soil samples. The result of samples collected from both dumpsites indicated a low hydraulic conductivity compared to samples collected 1.6 m from both dumpsites. Also, the chemical composition and pH of both dumpsite underlying soils indicated high level of soil contaminants with pH of 3.3 and 3.5 which is very acidic unlike pH of other samples which were in the neutral range (6.8-7.1). Hence, a liner is recommended for all dumpsites or engineered landfill systems to mitigate against the challenges associated with open waste dumping system in the environment.

• Journal of Ginseng Research
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• 제43권1호
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• pp.1-9
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• 2019

Background: Korean ginseng is an important cash crop in Asian countries. However, plant yield is reduced by pathogens. Among the Ilyonectria radicicola-species complex, I. mors-panacis is responsible for root-rot and replant failure of ginseng in Asia. The development of new methods to reveal the existence of the pathogen before cultivation is started is essential. Therefore, a quantitative real-time polymerase chain reaction method was developed to detect and quantify the pathogen in ginseng soils. Methods: In this study, a species-specific histone H3 primer set was developed for the quantification of I. mors-panacis. The primer set was used on DNA from other microbes to evaluate its sensitivity and selectivity for I. mors-panacis DNA. Sterilized soil samples artificially infected with the pathogen at different concentrations were used to evaluate the ability of the primer set to detect the pathogen population in the soil DNA. Finally, the pathogen was quantified in many natural soil samples. Results: The designed primer set was found to be sensitive and selective for I. mors-panacis DNA. In artificially infected sterilized soil samples, using quantitative real-time polymerase chain reaction the estimated amount of template was positively correlated with the pathogen concentration in soil samples ($R^2=0.95$), disease severity index ($R^2=0.99$), and colony-forming units ($R^2=0.87$). In natural soils, the pathogen was recorded in most fields producing bad yields at a range of $5.82{\pm}2.35pg/g$ to $892.34{\pm}103.70pg/g$ of soil. Conclusion: According to these results, the proposed primer set is applicable for estimating soil quality before ginseng cultivation. This will contribute to disease management and crop protection in the future.