• Korean Journal of Food Science and Technology
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• v.19 no.4
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• pp.324-330
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• 1987

A large number of methanol-assimilating yeasts and bacteria were isolated from samples of soil, sewage, decomposed milk and spoiled sweet-radish pickles. Among the yeasts, one strain was selected and identified as a strain of Candida boidinii. In 1% (v/v) methanol Candida boidinii YF-3 grew well and could grow in as much as 5%. This yeast required boitin for grwoth. Maximum growth was observed at $30^{\circ}C$ and pH 6 in a semisynthetic medium. The productivity was 2.72g dry cells per liter in batch culture with 1%(v/v) methanol and the cell yield for methanol was $0.39\;gg^{-1}$. The specific growth rate was $0.11\;h^{-1}$ and the generation time was 6.4 hours. The protein content of the cell was 45.5% and total nucleic acid content was 5.9%. The amino acid profile was as good as FAO standard for food protein.

• Economic and Environmental Geology
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• v.32 no.6
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• pp.633-644
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• 1999

Gully pot is a part of urban darainage system to butter the runoff water fluxes from road to rivers and to minimize environmental pollution by prevending over the surrounding area. A series of studies have been carried out concerning the physicochemical characteristics of the sewage sludge sediments sttling down in such a gully pot in order to over to evaluate the contamination for heavy metals such as pb, Zn, Cu and Cd. The roadside soil and sewage sludge samples from gully pots were characterized by XRD analyses and sequential extraction : Zn 2595.7$\mu$g/g; Cd9.8$\mu$/g; Cu602.5$\mu$g/g; Pb260.0$\mu$g/g),because of a long-term accumulation of vehicle- and industrial-related pollutants. Mean Zn concentration in Yeouido (3873$\mu$g/g) and Junggu(3262$\mu$g/g)areas are 4-5 times higher than those in Dobonggu area, suggesting that Zn may be derived from automobile traffic (including the rubber of automobile tires). The mean concentrations of Cu and Cr are very significantly high in Junggu and Gurogu areas, possibly due to the industrial activities in these areas. The low Pb levels throughtotut the whole study areas in Seoul can be accounted for the use of unleaded gasoline since 1987. Sequential extraction experiments illustrate that a major part of Zn is bound to FII and FIII, representing about 88% of the total Zn concentration. Fraction IV, related to orgnic matter, is mostly significant for Cu accounting for 60% of the total Cu, and FII is next in importance. The main carriers of Pb are the fractions III, II and FIV, and in that order. The behavior of Cd is quite different from other elements (Zn, Cu, Pb), and most of the Cd is associated with FV. Changes in the physicochemical environments (such as acidification) may result in severe environmental pollution of surface water and rivers with respect to heavy metals (especially Zn and Cu).

• Economic and Environmental Geology
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• v.37 no.3
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• pp.277-290
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• 2004

The trench exploration was carried out on the quartz veins of the Tanggung area of Java, Indonesia. The chemical values of quartz vein samples for hydrothermal alteration zones in this area are defined such as over values of Ag : 2∼190ppm(Celak); 2∼41 ppm(Cilangkap), Au : 21∼17,400 ppb(Celak); 24∼91,100 ppb(Cilangkap), Cu : 9∼3,340 ppm(Celak); 2∼l,600 ppm(Cilangkap), Pb : 60∼52,900 ppm(Celak); 16∼2,960 ppm(Cilangkap), Zn : 12∼13,950 ppm(Celak); 14∼1,520 ppm(Cilangkap), respectively. Two drilling areas, Mt. Subang area(Celak area) and Mt. Cilangkap area(Cilangkap area), have been chosen based on the factors such as, geologic settings associated with gold mineralizations, highly anomalous areas revealed by geochemical survey such as chemical analysis of ore and soil, trench exploration, statistical processing of the chemical analysis, and petrographic studies of igneous rocks related to its mineralization, and graphic presentation of anomalous areas.

• Journal of Radiation Industry
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• v.3 no.1
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• pp.39-45
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• 2009

Three mineral phosphate solubilizing (MPS) bacteria where isolated from rhizosphere soil samples of common bean and weed plants. 16S rDNA analysis indicated that the isolate P2 and P3 are closely related to Pantoea dispersa while isolate P4 is closely related to Pantoea terrae. Isolates P2 and P3 recorded $381.60{\mu}g\;ml^{-1}$ and $356.27{\mu}g\;ml^{-1}$ of tricalcium phosphate (TCP) solubilization respectively on 3 days incubation. Isolate P4 recorded the TCP solubilization of $215.85{\mu}g\;ml^{-1}$ and the pH was dropped to 4.44 on 24 h incubation. Further incubation of P4 sharply decreased the available phosphorous to $28.94{\mu}g\;ml^{-1}$ and pH level was raised to 6.32. Gamma radiation induced mutagenesis was carried out at $LD_{99}$ dose of the wild type strains. The total of 14 mutant clones with enhanced MPS activity and 4 clones with decreased activity were selected based on solubilization index (SI) and phosphate solubilization assay. Mutant P2-M1 recorded the highest P-solubilizing potential among any other wild or mutant clones by releasing $504.21{\mu}g\;ml^{-1}$ of phosphorous i.e. 35% higher than its wild type by the end of day 5. A comparative evaluation of TCP solubilization by wild type isolates of Pantoea and their mutants, led to select three MPS mutant clones such as P2-M1, P3-M2 and P3-M4 with a potential to release >$471.67{\mu}g\;ml^{-1}$ of phosphorous from TCP. These over expressing mutant clones are considered as suitable candidates for biofertilization.

• Korean Journal of Remote Sensing
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• v.21 no.6
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• pp.493-503
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• 2005

In this study, the weight of evidence(WofE) technique based on GIS was applied to spatially estimate the groundwater yield characteristics at the Pocheon area In Gyunggi-do. The groundwater preservation depends on many hydro-geologic factors that include hydrologic data, land-use data, topographic data, geological map and other natural materials collected at the site, even with man-made things. All these data can be digitally processed and managed by GIS database. In the applied technique of WofE, the prior probabilities were estimated as the factors that affect the yield on lineament, geology, drainage pattern or river system density, landuse and soil. We calculated the value of the weight values, W+ and W-, of each factor and estimated the contrast value of it. Results by the groundwater yield characteristic computation using this scheme were presented feasibility map in the form of the posterior probability to the consideration of in-situ samples. It is concluded that this technique is regarded as one of the effective techniques for the feasibility mapping related to the estimation of groundwater-bearing potential zones and its spatial pattern.

• Journal of The Geomorphological Association of Korea
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• v.23 no.3
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• pp.79-92
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• 2016

Samples from two boreholes of coastal dune field at Jangho-ri coast, Gochang was studied. These were analyzed by grain size analysis geochemical analysis, and the application of OSL dating method to understand the development during the Holocene. The boreholes SB8 and SB9 were classified into three different sedimentary layers by their mean grain size and geochemical characteristics. The results revealed that the upper sand layer is equivalent to the present coastal dune layer, which developed since 1,200 years ago; the silt layer in the middle to the dune slack or lagoon sedimentation layer, which developed between 1,200 and 6,000 years ago; and the sand layer at the bottom to the paleo coastal dune that developed between 6,000 and 7,000 years ago. It was proposed that the forming material of current coastal dune was supplied from the sandy flat in coastal area, while the middle silt layer was supplied from the weathered soil of a bed rock by the comparison with material of surrounding area. In the case of coastal dune, concentrated layer of sands were identified which were buried about 300 and 1,200 years ago, which is identified as the little ice age. This study confirmed the development of Jangho-ri coastal dunes after Holocene Climate Optimum period, and it is likely to assist in the understanding of coastal dunes development.

• Journal of Korean Society on Water Environment
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• v.37 no.1
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• pp.20-31
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• 2021

In this study, an analysis of the characteristics of organic matter and nitrogenous oxygen demand (NOD) of 17 sewage effluent and wastewater treatments was conducted. High CODMn and carbonaceous biological oxygen demand (CBOD) concentrations were observed in the livestock treatment plants (LTP), wastewater treatment plants(WTP), and night soil treatment plants (NTP), but the highest NOD concentration and contribution rates of NOD to BOD5 were found in sewage treatment plants (STP). There was no significant difference in the CBOD/CODMn ratio for each of the six pollution source groups, but the LTPs, WTPs, and NTPs all showed relatively high CODMn concentrations in their effluent samples, indicating that they are facilities which discharge large amounts of refractory organic matter. The seasonal change of NOD in all facilities' effluent was found to be larger than the seasonal change of CBOD, and data results also revealed an elevation of NOD and NH3-N concentration from December to February, when the water temperature was low. There was no significant difference in NH3-N concentration in relation to pollution source group (p=0.08, one-way ANOVA), but the STP, which had a high NOD contribution rate to BOD5 of 48%, showed a high correlation between BOD5 and NOD (r2=0.95, p<0.0001). These results suggest that the effect of NOD on BOD5 is an important factor to be considered when analyzing STP effluent.

• Journal of Climate Change Research
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• v.6 no.1
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• pp.33-40
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• 2015

Forests are to be recognized as an important carbon sink under the UNFCCC that consist of above- and below-biomass, dead organic matter (DOM) such as dead wood and litter, and soil organic matter (SOM). In order to asses for DOM and SOM, however, it is relevant to land-use change matrices over last 20 years for each land-use category. In this study, a land-use change matrix was produced and its uncertainty was assessed using a point sampling technique with permanent sample plots in national forest inventory at Chungbuk province. With point sampling estimated areas at 2012 year for each land-use category were significantly similar to the true areas by given six land-use categories. Relative standard error in terms of uncertainty of land-use change among land-use categories ranged in 4.3~44.4%, excluding the other land. Forest and cropland covered relatively large areas showed lower uncertainty compared to the other land-use categories. This result showed that selected permanent samples in the NFI are able to support for producing land-use change matrix at a national or province level. If the $6^{th}$ NFI data are fully collected, the uncertainty of estimated area should be improved.

• Microbiology and Biotechnology Letters
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• v.46 no.4
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• pp.346-359
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• 2018

Xanthomonas oryzae, a bacterial pathogen causing leaf blight disease (BLB) in rice, can cause widespread disease and has caused epidemics globally, resulting in severe crop losses of 50% in Asia. The pathogen is seed-borne and is transmitted through seeds. Thus, control of BLB requires the elimination of the pathogen from seeds. Concern about environment-friendly organic production has spurred improvements in a variety of biological disease control methods, including the use of bacteriophages, against bacterial plant pathogens. The present study explored the potential of bacteriophages isolated from diseased plant leaves and soil samples in killing the bacterial pathogen in rice seeds. Eight different phages were isolated and evaluated for their bacteriolytic activity against different pathogenic X. oryzae strains. Of these, a phage designated ${\varphi}XOF4$ killed all the pathogenic X. oryzae strains and showed the broadest host range. Transmission electron microscopy of ${\varphi}XOF4$ revealed it to be a tailed phage with an icosahedral head. The virus was assigned to the family Siphoviridae, order Caudovirales. Seedlings raised from the seeds treated with $1{\times}10^8pfu/ml$ of ${\varphi}XOF4$ phage displayed reduced incidence of BLB disease and complete bacterial growth inhibition. The findings indicate the potential of the ${\varphi}XOF4$ phage as a potential biological control agent against BLB disease in rice.

• Journal of Species Research
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• v.8 no.2
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• pp.176-190
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• 2019

A total of 38 bacterial strains within the classes Bacilli and Deinococci were isolated from various sources in Korea. Samples were collected from animal intestine, urine, soil, tidal flat mud, and kimchi. In the sequence comparison and phylogenetic analysis of 16S rRNA sequences, the 38 isolates were assigned to the classes Bacilli and Deinococci with sequence similarities more than 98.7%. Twenty-four strains and 13 strains were classified the order Bacillales and Lactobacillales in the class Bacilli, respectively. In the order Bacillales, there were nine species in the genus Bacillus, seven species in the genus Paenibacillus, and the remaining eight species in the genera Domibacillus, Halobacillus, Virgibacillus, Lysinibacillus, Paenisporosarcina, Planococcus, Savagea, and Staphylococcus. In the order Lactobacillales, there were four species in the genus Lactobacillus, three species in the genus Leuconostoc, three species in the genus Lactococcus, and the remaining three species in the genera Aerococcus, Enterococcus, and Streptococcus. One species was related to the genus Deinococcus of the order Deinococcales. Most of the isolated strains were Gram-stain-positive, but some were Gram-stain-variable or Gram-stain-negative. Cells were rod or cocci-shaped. Based on the results of 16S rRNA analysis, we report 38 strains as previously unrecorded species to Korea, and the basic characteristics of strains are described herein.