• Title/Summary/Keyword: soil microbes

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On the Decay Rate of Soil Organic Matter and Changes of Soil Microbial populaiton (토양유기물의 분해속도와 Microbial populaiton의 소장에 관한 연구)

  • 김춘민
    • Journal of Plant Biology
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    • v.10 no.1_2
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    • pp.21-30
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    • 1967
  • The aim of present study is to elucidate the relationship between decay rate of soil organic matter, and the change of soil microbial population under the oak and pine forest soils in Kwang-nung plantation stand. The results obtained are as follows: 1) The correlation coefficient between decay rate and the soil bacteria is 0.84 and fungi 0.93. 2) The distribution of soil microbial population is higher in both F horizon of the oak forest soil, and F and H horizon of the pine forest soil. However, the number of soil microorganisms decreases with the depth in each forest soil. 3) The population of soil microbes is related to moisture content, total nitrogen, available phosphorus, and exchangeable calcium, except organic carbon in fungi. 4) The soil organic matter has been mainly decomposed by fungi, and the size of its population are governed by the factors such as moisture content, organic carbon, total nitrogen, available phosphorus, and exchangeable calcium.

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An Assessment of the Feasibility of (I) : Condition of Aerobic (MTBE를 포함한 기타 가솔린 첨가제의 생 분해 적용 가능성 평가(I) : 호기성 조건)

  • Chung, Woo-jin;Chang, Soon-woong
    • Journal of Environmental Science International
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    • v.25 no.6
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    • pp.757-766
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    • 2016
  • MTBE and other gasoline additives contained in gasoline are known to be a refractory substance resistant to biodegradation. As a method of removing these substances, a research of method using native microbes of polluted soil was progressed and among these, bio-degradation possibility under aerobic condition was evaluated. All of the experiments were progressed based on batch experiment of lab scale and analyzed by GC-FID using HS-SPME technique. The result of bio-degradation experiment based on MTBE and other additives(ETBE, TAME) was observed below 1 mg/L, which initial concentration were 100 mg/L for each method. And through production of by-product and CO2, partial mineralization was confirmed. Degradation velocity of each additive was promptly represented in the order of TBA>ETBE>MTBE>TAME. Through this study, bio-degradation possibility of native microbes of oil polluted soil, MTBE and other gasoline additives was confirmed and it was considered that the result could be used for basic experiment data in removing oil pollutants of soil.

Studies on Antibiotic Producers of Korean Soil Microbes(I) -Isolation and Antibiotic Activity of Streptomyces Strain DMC-72- (한국(韓國) 토양균(土壤菌)중 항생물질(抗生物質) 생성균(生成菌)에 관한 연구(硏究)(제1보)(第1報) -스트렙토마이세스속(屬) 균주(菌株) DMC-72호(號)의 분리(分離) 및 항균작용(抗菌作用)-)

  • Kim, Kwang-Wook;Choi, Eung-Chil;Shim, Mi-Ja;Kim, Byong-Kak
    • Korean Journal of Pharmacognosy
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    • v.15 no.1
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    • pp.15-23
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    • 1984
  • To find antimicrobial strains of the soil microorganisms in Korea, they were isolated from the soil samples of different locations and screened for antibiotic activity against several standard microbes. An isolate among them had an antibacterial activity against gram-positive bacteria. The examination of its morphological and biochemical characteristics according to the International Streptomyces Project methods showed that it belongs to the genus Streptomyces. The strain was named DMC-72. The strain appears to be a new strain when it was compared with the species within the genus which have been so far reported. The antibiotic metabolite of the strain was produced in submerged culture method. It was found to be a quinone compound and was named soulomycin. This strain was also found to produce an ${\alpha}-amylase$ inhibitor in the submerged culture.

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Effects of Experimental Drought on Soil Bacterial Community in a Larix Kaempferi Stand

  • Kim, Beomjeong;Choi, Byoungkoo
    • Journal of Forest and Environmental Science
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    • v.34 no.3
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    • pp.258-261
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    • 2018
  • Drought alters soil microorganisms; however, it is still not clear how soil microbes respond to severe drought conditions. In this study, the responses of soil bacterial community to experimental drought in a coniferous stand were examined. Six $6m{\times}6m$ plots with three replicates of control and drought treatments were delimited. PCR amplification and Illumina sequencing were conducted for cluster analysis of soil bacterial community and species richness and species diversity was analyzed. Along the 393 days of simulated drought from July 2016 to October 2017, soil bacterial species diversity slightly increased whereas species richness decreased in both control and roof plots. Moreover, soil bacterial species richness more decreased in roof plots than in controls. Combining these results, soil bacterial activity might have been altered by simulated drought.

Effects of Amendments on the Phosphate-solubilizing Bacteria in Rice Paddy Soils (논 토양 인산가용화세균에 대한 개량제 시용효과)

  • Suh, Jang-Sun;Noh, Hyung-Jun;Kwon, Jang-Sik
    • Korean Journal of Soil Science and Fertilizer
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    • v.41 no.5
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    • pp.342-347
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    • 2008
  • Phosphate soubilized by microbes can be easily absorbed by plant as the element diffuses into soil solution. The microbes related to phosphate solubilizing activity are affected by the soil amendments such as rice straw compost, and lime. This study was performed to evaluate the effect of amendments to phosphate solubilizer in rice paddy soils. Available phosphate concentration was increased with the ratio of phosphate-solubilizing bacteria to aerobic bacteria in the rice paddy soils. The ratio was high in the plots applied with lime, silicate, and rice straw compost. Phosphate-solubilizing bacteria isolated from the soil were Aquasipirillum, Arthrobacter, Bacillus, Flavobacterium, Micrococcus and Micromonospora, Pseudomonas species. The highest dominant bacterial species was Pseudomonas, and Bacillus was followed.

Effect of Homemade Liquid Fertilizers on Chemical Property and Microbial Activity of Soil and Cucumber Growth (자가제조 액비처리가 토양 화학성과 미생물상 및 오이의 생장에 미치는 영향)

  • Jung, Ji-Sik;Jung, Seok-Kyu;Choi, Hyun-Sug
    • Journal of the Korea Organic Resources Recycling Association
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    • v.27 no.3
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    • pp.15-25
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    • 2019
  • The study was conducted to compare the chemical properties and microbial activity of soil and the crop productivity by applying homemade liquid fertilizers (LF) used in leading cucumber farms as well as to evaluate the eco-friendly LFs to substitute for a chemical fertilizer. Three homemade LFs, EM, starfish, and native microbes, and a chemical LF were regularly fertigated per three days during the growing season. Chemical LF contained the highest pH, EC (electrical conductivity), and concentrations of T-N, $P_2O_5$, K, Ca, and Mg, while the lowest EC level was observed for EM LF. Soil EC was the highest to the 3.0 dS/m for chemical LF-plots, with lowering soil pH, OM (organic matter), and Mg concentration. Soil chemical properties mostly increased in native microbes LF-plots. However, soil microbial properties were not significantly different among the LF treatment plots. OTU (operational taxonomic units), richness estimator, and diversity index of bacteria and fungi increased in the chemical LF and EM LF based on the pyrosequencing analysis. SPAD and PS II values on the treated-cucumber leaves were seasonally decreased from 32 to 60 days after transplanting, with the rapid decline observed at 45 days after transplanting. Number of leaves and crop height increased in the treatments with EM and native microbes LF. LF treated-cucumber crops were not significantly different for total fresh weight and fruit yield.

Effects of Short-Term Oxygen Exposure on Anaerobic Reductive Dechlorination and Formate Fermentation by Evanite Culture (혐기성탈염소화 혼합균주에서 산소 노출이 탈염소화 및 수소발생 발효에 미치는 영향)

  • Hong, Ui-Jeon;Park, Sun-Hwa;Lim, Jong-Hwan;Ahn, Hong-Il;Kim, Nam-Hee;Lee, Suk-Woo;Kim, Young
    • Journal of Soil and Groundwater Environment
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    • v.15 no.6
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    • pp.114-121
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    • 2010
  • Oxygen sensitivity and substrate requirement have been known as possible reasons for the intricate growth of Dehalococcoides spp. and limiting factors of for routinely applying bioaugmentation using anaerobic Dehalococcoides-containing microbes for remediating chlorinated organic compounds. To explore the effect of the short-term exposure of the short-term exposure of oxygen on Dehalococcoides capability, dechlorination performance, and hydrogen production fermentation from formate, an anaerobic reductive dechlorination mixed-culture (Evanite culture) including dehalococcoides spp. was in this study. In the results, once the mixed-culture were exposed to oxygen, trichloroethylene (TCE) degradation rate decreased and it was not fully recovered even addition of excess formate for 40 days. In contrast, hydrogen was continuously produced by hydrogen-fermentation process even under oxygen presence. The results indicate that although the oxygen-exposed cells cannot completely dechlorinate TCE to ethylene (ETH), hydrogen fermentation process was not affected by oxygen presence. These results suggest that dechlorinating microbes may more sensitive to oxygen than fermenting microbes, and monitoring dechlorinators activity may be critical to achieve an successful remediation of a TCE contaminated-aquifer through bioaugmentation using Dehalococcoides spp..

Enzyme Activities in the Soil of Quercus mongolica Forests (신갈나무 산림토양에서의 효소활성도)

  • Song In-Geun;Yong-Keel Choi;Byung-Re Min
    • The Korean Journal of Ecology
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    • v.18 no.4
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    • pp.503-512
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    • 1995
  • The present paper describes partial results of the study on the activities of microbes in the soil of Quercus mongolica forest from July, 1994 to April, 1995. To determine the relationship between structure and function of soil microbial ecosystem, the author investigated the seasonal change of physical environmental factors, microbial population and soil enzyme activities. The changes of pH was not significant and the temperature of surface soil was 2℃ higher than lower soil through out the year. Moisture contents (%) of soil samples ranged from 7.64% to 42.11%. However, soils of site 3 at Mt. Komdan in which vegetation is successional have higher moisture content than the others. The bacterial population increased in summer, but continuously decreased in autumn and winter, and then reincreased again in spring. Bacterial population of surface soil was higher than those of 30 cm depth all the year round. Dehydrogenase activity (DHA) was about two-fold higher throughout in surface soil compared to those of lower soil. And the correlation coefficient between DHA and bacterial population size was 0,713, It was suggested that DHA could be used as a primary index of soil microbial population and activity in soil ecosystem.

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Arsenic Detoxification by As(III)-Oxidizing Bacteria: A Proposition for Sustainable Environmental Management

  • Shamayita Basu;Samir Kumar Mukherjee;Sk Tofajjen Hossain
    • Microbiology and Biotechnology Letters
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    • v.51 no.1
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    • pp.1-9
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    • 2023
  • Arsenic (As), which is ubiquitous throughout the environment, represents a major environmental threat at higher concentration and poses a global public health concern in certain geographic areas. Most of the conventional arsenic remediation techniques that are currently in use have certain limitations. This situation necessitates a potential remediation strategy, and in this regard bioremediation technology is increasingly important. Being the oldest representativse of life on Earth, microbes have developed various strategies to cope with hostile environments containing different toxic metals or metalloids including As. Such conditions prompted the evolution of numerous genetic systems that have enabled many microbes to utilize this metalloid in their metabolic activities. Therefore, within a certain scope bacterial isolates could be helpful for sustainable management of As-contamination. Research interest in microbial As(III) oxidation has increased recently, as oxidation of As(III) to less hazardous As(V) is viewed as a strategy to ameliorate its adverse impact. In this review, the novelty of As(III) oxidation is highlighted and the implication of As(III)-oxidizing microbes in environmental management and their prospects are also discussed. Moreover, future exploitation of As(III)-oxidizing bacteria, as potential plant growth-promoting bacteria, may add agronomic importance to their widespread utilization in managing soil quality and yield output of major field crops, in addition to reducing As accumulation and toxicity in crops.

Crop Rotation in Paddy Soil Exhibiting Crop Failure Following Replanting: Effect on Soil Chemical Properties, Soil Microbial Community and Growth Characteristics of 2-Year-Old Ginseng (인삼 논재배 연작지에서 윤작물 재배가 토양화학성, 토양 미생물상 및 2년생 인삼의 생육에 미치는 영향)

  • Lee, Sung Woo;Park, Kyung Hoon;Lee, Seung Ho;Jang, In Bok;Jin, Mei Lan
    • Korean Journal of Medicinal Crop Science
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    • v.24 no.4
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    • pp.294-302
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    • 2016
  • Background: Crop rotation plays an important role in improving soil chemical properties, minimizing the presence of disease pathogens, and assists in neutralizing autotoxic effects associated with allelochemicals. Methods and Results: Five rotation crops of sudan grass, soybean, peanut, sweet potato, and perilla were cultivated for one year with an aim to reduce yield losses caused by repeated cropping of ginseng. In 2-year-old ginseng grown in the same soil as a previous ginseng crop, stem length and leaf area were reduced by 30%, and root weight per plant was reduced by 56%. Crop rotation resulted in a significant decrease in electrical conductivity, $NO_3$, and $P_2O_5$ content of the soil, whereas organic matter, Ca, Mg, Fe, Cu, and Zn content remained-unchanged. Soil K content was increased following crop rotation with sudan grass and peanut only. Rotation with all alternate crops increased subsequent ginseng aerial plant biomass, whereas root weight per plant significantly increased following crop rotation with perilla only. A significant positive correlation was observed between root rot ration and soil K content, and a significant negative correlation was observed between ginseng root yield and the abundance of actinomycetes. Crop rotation affected the soil microbial community by increasing gram negative microbes, the ratio of aerobic microbes, and total microbial biomass whereas decreases were observed in actinomycetes and the ration of saturated fatty acids. Conclusions: In soil exhibiting crop failure following replanting, crop rotation for one year promoted both soil microbial activity and subsequent ginseng aerial plant biomass, but did not ameliorate the occurrence of root rot disease.