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Mechanism study on DNA damage and Apoptosis induced by heak shock using Comet Assay

  • Seo, Young-Rok;Han, Sung-Sik;Kim, L. O′Neill;Ryu, Jae-Chun
    • Proceedings of the Korea Society of Environmental Toocicology Conference
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    • 1997.12a
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    • pp.101-101
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    • 1997
  • Comet assay, single cell gel electrophoresis has been known as useful, rapid, simple, visual, and sensitive technique for measuring the DNA breakage in mammalian ce1ls. For evaluation of DNA damage using comet assay, early studies reported a change in comet length and intensity with DNA damage using simple visual technique, such as fluorescence microscopy with eyespiece. In recent, some workers are observing and analyzing nucleotide of comets using quantitative fluorescence image analysis system to estimate 'tail moment', which is defined as the product of the tail length and the fraction of total DNA in tail. Our laboratory also adopted the image analysis software for qualification. In addition, many of the practical features of comet assay render it potentially attractive as useful tool for molecular toxicology and carcinogenesis, because the system is already showing considerable promise as rapid predictor in both in vitro and in vivo experimental designs. Recently, the comet assay becomes a attractive technique to study of apoptosis, because apoptotic fragmentation of nuclear DNA into nucleosomal sizes can be evaluated by the comet assay. So, we attempted to apply the comet assay to studying the effect of various stress on the apoptosis-sensitive cell lines. Particularly, focusing on the hyperthermic apoptosis, we could find that heat shock(44˚C for 60 minutes) was sufficient to induced apoptosis in these cell lines. But using the highly sensitive comet assay, we could not detect DNA breaks immediately after heat shock.

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The Direction for Fashion Merchandising Education (패션 머천다이징 교육(敎育) 방향(方向))

  • Chun, Hei-Jung
    • Journal of Fashion Business
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    • v.4 no.1
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    • pp.87-96
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    • 2000
  • Merchandiser continue to play an important role in the exchange process by providing products for consumption. Merchandisers must still understand customer demands, analyze sale trends, select and present salable products. However, due to the competitive pressures in the apparel industry and the innovations required under QR business systems, the demands placed on merchandisers are changing. The purpose of this study is to present of the direction for fashion merchandising education. The direction for fashion merchandising are education summarized as follows; 1) Merchandising technology is the systematic application of information technology and Telecomunications to planning, developing, and presenting product lines in ways that reflect social and cultural value. Statistic Methods are developed and used to analyze data arising from a wide variety of applications. 2) Merchandising technology is to practise the technical and economic aspects of apparel production. Analysis of specific apparel manufacturing and management issues such as efficient manufacturing methods. 3) Merchandising technology is to forecast fashion trend according to consumer preference. Culture influences what people purchase and how those items are used forecasting fashion trend. 4) Merchandising technology is to practise communication skills used in formal and informal organization including interviews in particular language suited to their own business and professionnal careers. 5) Merchandising technology is to planning merchandise budgets and merchandise assortments based on more diverse forms of information. 6) Merchandising technology is to use techniques related hardware and software. 7) Merchandising technology is to learn participate in internship programs.

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Fault-Causing Process and Equipment Analysis of PCB Manufacturing Lines Using Data Mining Techniques (데이터마이닝 기법을 이용한 PCB 제조라인의 불량 혐의 공정 및 설비 분석)

  • Sim, Hyun Sik;Kim, Chang Ouk
    • KIPS Transactions on Software and Data Engineering
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    • v.4 no.2
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    • pp.65-70
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    • 2015
  • In the PCB(Printed Circuit Board) manufacturing industry, the yield is an important management factor because it affects the product cost and quality significantly. In real situation, it is very hard to ensure a high yield in a manufacturing shop because products called chips are made through hundreds of nano-scale manufacturing processes. Therefore, in order to improve the yield, it is necessary to analyze main fault process and equipment that cause low PCB yield. This paper proposes a systematic approach to discover fault-causing processes and equipment by using a logistic regression and a stepwise variable selection procedure. We tested our approach with lot trace records of real work-site. A lot trace record consists of the equipment sequence that the lot passed through and the number of faults for each fault type in the lot. We demonstrated that the test results reflected the real situation of a PCB manufacturing line.

E-Business and Simulation

  • Park, Sung-Joo
    • Proceedings of the Korea Society for Simulation Conference
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    • 2001.10a
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    • pp.9-10
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    • 2001
  • Simulation has been evolved with the advance of computer and technique of modeling application systems. Early simulations were numerical analysis of engineering models known as continuous simulation, analysis of random events using various random number generators thus named as Monte Carlo simulation, iud analysis o(\\\\`queues which are prevalent in many real world systems including manufacturing, transportation, telecommunication. Discrete-event simulation has been used far modeling and analyzing the systems with waiting lines and inefficient delays. These simulations, either discrete-event, continuous, or hybrid, have played a key role in industrial age by helping to design and implement the efficient real world systems. In the information age which has been brought up by the advent of Internet, e-business has emerged. E-business, any business using Internet, can be characterized by the network of extended enterprises---extended supply and demand chains. The extension of value chains spans far reaching scope in business functions and space globally. It also extends to the individual customer, customer preferences and behaviors, to find the best service and product fit for each individual---mass customization. Simulation should also play a key role in analyzing and evaluating the various phenomena of e-business where the phenomena can be characterized by dynamics, uncertainty, and complexity. In this tutorial, applications of simulation to e-business phenomena will be explained and illustrated. Examples are the dynamics of new economy, analysis of e-business processes, virtual manufacturing system, digital divide phenomena, etc. Partly influenced by e-business, a new trend of simulation has emerged called agent-based simulation, Agent-based simulation is a technique of simulation using software agent that have autonomy and proactivity which are useful in analyzing and integrating numerous individual customer's behavior. One particular form of agent-based simulation is swarm. This tutorial concludes with the illustration of swarm or swarm Intelligence applied to various e-business applications, and future directions and implications of this new trend of simulation.

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A Hybrid Information Retrieval Model Using Metadata and Text (메타데이타와 텍스트 정보의 통합검색 모델)

  • Yoo, Jeong-Mok;Myaeng, Sung-Hyon;Kim, Sung-Soo;Lee, Mann-Ho
    • Journal of KIISE:Databases
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    • v.34 no.3
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    • pp.232-243
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    • 2007
  • Metadata IR model has high precision and low recall because the query in Metadata IR model is strict that is, the query can express user information need exactly, while Full-text IR model has low precision and high recall because the query in Full-text IR model is a kind of simple keyword query which expresses user information need roughly. If user can translate one's information need into structured query well, the retrieval result will be improved. However, it is little possible to make relevant query without understanding characteristics of metadata. Unfortunately, most users do not interested in metadata, then they cannot construct well-made structured query. Amount of information contained in metadata is less than text information. In this paper, we suggest hybrid IR model using metadata and text which can provide users with lots of relevant documents by retrieving from metadata field and text field complementarily.

An Interface Technique for Avatar-Object Behavior Control using Layered Behavior Script Representation (계층적 행위 스크립트 표현을 통한 아바타-객체 행위 제어를 위한 인터페이스 기법)

  • Choi Seung-Hyuk;Kim Jae-Kyung;Lim Soon-Bum;Choy Yoon-Chul
    • Journal of KIISE:Software and Applications
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    • v.33 no.9
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    • pp.751-775
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    • 2006
  • In this paper, we suggested an avatar control technique using the high-level behavior. We separated behaviors into three levels according to level of abstraction and defined layered scripts. Layered scripts provide the user with the control over the avatar behaviors at the abstract level and the reusability of scripts. As the 3D environment gets complicated, the number of required avatar behaviors increases accordingly and thus controlling the avatar-object behaviors gets even more challenging. To solve this problem, we embed avatar behaviors into each environment object, which informs how the avatar can interact with the object. Even with a large number of environment objects, our system can manage avatar-object interactions in an object-oriented manner Finally, we suggest an easy-to-use user interface technique that allows the user to control avatars based on context menus. Using the avatar behavior information that is embedded into the object, the system can analyze the object state and filter the behaviors. As a result, context menu shows the behaviors that the avatar can do. In this paper, we made the virtual presentation environment and applied our model to the system. In this paper, we suggested the technique that we controling an the avatar control technique using the high-level behavior. We separated behaviors into three levels byaccording to level of abstract levelion and defined multi-levellayered script. Multi-leveILayered script offers that the user can control avatar behavior at the abstract level and reuses script easily. We suggested object models for avatar-object interaction. Because, TtThe 3D environment is getting more complicated very quickly, so that the numberss of avatar behaviors are getting more variableincreased. Therefore, controlling avatar-object behavior is getting complex and difficultWe need tough processing for handling avatar-object interaction. To solve this problem, we suggested object models that embedded avatar behaviors into object for avatar-object interaction. insert embedded ail avatar behaviors into object. Even though the numbers of objects areis large bigger, it can manage avatar-object interactions by very efficientlyobject-oriented manner. Finally Wewe suggested context menu for ease ordering. User can control avatar throughusing not avatar but the object-oriented interfaces. To do this, Oobject model is suggested by analyzeing object state and filtering the behavior, behavior and context menu shows the behaviors that avatar can do. The user doesn't care about the object or avatar state through the related object.