• Title/Summary/Keyword: socD500

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Myxococcus xanthus socD500 mutation causes Sporulation and Induction of two C-signal Specific Genes (Myxococcus xanthus socD500에 의한 포자 형성 및 CsgA신호에 특이적 유전자의 발현에 관한 연구)

  • 이병욱
    • Journal of Life Science
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    • v.13 no.2
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    • pp.184-190
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    • 2003
  • Myxococcus xanthus is a Gram negative, rod-shaped, soil bacterium that displays a social behaviors, and multicellular development upon nutrient deprivation. The csgA gene encoding a cell surface protein is essential for developmental behaviors including rippling, aggregation, fruiting body formation and sporulation. csgA mutants show normal vegetative growth, but lack all these developmental phenotypes. Expression of the CsgA (C-signal) specific genes are eliminated or dramatically reduced in csgA mutants. In order to identify components of C-signal transduction pathway, second site mutations were introduced into csgA mutants and were identified which can fully or partially restore development of csgA mutants (Rhie, H. G. et. al. 1989. J. Bacteriol. 171, 3268-3276). One of such csgA suppressor mutations, socD500 restores only sporulation to csgA mutants at 15$^{\circ}C$. The socD500 mutaion however eliminates the three basic developmental requirements, starvation, high cell density and a solid surface. Only sporulation, not accompanied with fruiting body formation is induced simply by shifting the temperature of vegetatively growing cells from $32^{\circ}C$ to $15^{\circ}C$. Spores induced by socD500 mutation is not as thick as that of wild-type fruiting body. In socD500 genetic background, two of ten C-signal dependent genes, $\Omega$DK4506 and $\Omega$DK4406 are more highly expressed in growing cells at $15^{\circ}C$. These results indicate that the socD500 mutation may be partly involved in the regulation of expression of two C-signal dependent genes and genes for sporulation in this transduction pathway.

VERIFICATION ON THE PERFORMANCE OF COMS SOC S-BANDSSPA

  • Park, Durk-Jong;Yang, Hyung-Mo;Ahn, Sang-Il
    • Proceedings of the KSRS Conference
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    • 2007.10a
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    • pp.482-485
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    • 2007
  • The S-Band SSPA is front-end equipment to transmit both LRIT and HRIT to COMS. To provide the required EIRP, S-Band SSPA is designed to generate maximum 1kW power at its 1dB gain compression point (P1dB). Due to the operation for 24 hours per seven days, the verification on the performance of S-Band SSPA was performed thoroughly. This paper presents that major requirements such as maximum 1kW power, maximum -26dBc of IMD characteristic at 500W output and around -57dBc of coupling factor were verified through proposed test configuration.

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How to Improve R&D Pre-Feasibility Study System (연구개발 분야 예비타당성 제도의 개선방향)

  • Choi, Seok-Joon;Kahn, Hyung-Sik
    • Journal of Korea Technology Innovation Society
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    • v.11 no.2
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    • pp.287-313
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    • 2008
  • Even though the pre-feasibility study was introduced in 1998, it mainly has focused on transportation social overhead capital(SOC) such as road and railroad. Last year, however, the government newly launched R&D project area for the pre-feasibility study. This paper tries to analyze the pre-feasibility study methodology and system that applied to R&D facility, cluster, and sized R&D infrastructure projects. In depth study of economic, policy, and AHP analysis, this paper shows that the preliminary feasibility study have some caveats and suggests a way of improvement for making up for limitation. Main suggestions are as follows; First, to improve the fit in R&D sector analysis, using consistent method is important. Second, technology analysis is essential for R&D evaluation. Third, technology experts should take part in AHP analysis.

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Analysis on Propagation of Highway Traffic Flow Turbulence at Entrance-Ramp Junctions (교통류 난류현상을 이용한 고속도로 합류부의 영향권 분석)

  • Lee, Ki Yoon;Roh, Chang Gyun;Son, BongSoo;Chung, Jin-Hyuk
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.29 no.2D
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    • pp.167-173
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    • 2009
  • In general, an influenced area of merging section is defined as 500 m including 100 m upstream and 400 m downstream. However, from an observation of the actual traffic flow, it is found that merging effect influences more on downstream than upstream. In this study, an influenced area of merging section on freeway is analyzed by using turbulence which is defined as conflicts between vehicles. In order to overcome the limits of existing traffic flow detection system established with intervals of about 500 m, this study uses raw data collected from the detectors which are established in entrance ramps with similar road conditions. To divide data of each point into similar road conditions, the data of total 72 hours is sorted by Level of Service. An influenced area analyzed by standard deviation of speed is 700 m section of highway, including 300 m upstream and 400 m downstream, for both right and left ramps. It is the result including upstream 200 m more than previous studies.