• Genomics & Informatics
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• 제7권1호
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• pp.26-31
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• 2009

Heat shock proteins are a class of molecular chaperones that can be found in nearly all organisms from Bacteria, Archaea and Eukarya domains. Heat shock proteins experience increased transcription during periods of heat induced osmotic stress and are involved in protein disaggregation and refolding as part of a cell's danger signaling cascade. Heat shock protein, Hsp20 is a small molecular chaperone that is approximately 20kDa in weight and is hypothesized to prevent aggregation and denaturation. Hsp20 can be found in several strains of Proteobacteria, which comprises the largest phyla of the Bacteria domain and also contains several medically significant bacterial strains. Genomic analyses were performed to determine a common evolutionary pattern among Hsp20 sequences in Proteobacteria. It was found that Hsp20 shared a common ancestor within and among the five subclasses of Proteobacteria. This is readily apparent from the amount of sequence similarities within and between Hsp20 protein sequences as well as phylogenetic analysis of sequences from proteobacterial and non-proteobacterial species.

• Environmental Analysis Health and Toxicology
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• 제23권4호
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• pp.231-245
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• 2008

Various methods for detecting changes in response behaviors of indicator specimens are presented for monitoring effects of toxic treatments. The movement patterns of individuals are quantitatively characterized by statistical (i.e., ANOVA, multivariate analysis) and computational (i.e., fractal dimension, Fourier transform) methods. Extraction of information in complex behavioral data is further illustrated by techniques in ecological informatics. Multi-Layer Perceptron and Self-Organizing Map are applied for detection and patterning of response behaviors of indicator specimens. The recent techniques of Wavelet analysis and line detection by Recurrent Self-Organizing Map are additionally discussed as an efficient tool for checking time-series movement data. Behavioral monitoring could be established as new methodology in integrative ecological assessment, tilling the gap between large-scale (e.g., community structure) and small-scale (e.g., molecular response) measurements.

• 한국수산과학회지
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• 제31권1호
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• pp.37-44
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• 1998

1994년 1월부터 1994년 12월까지 광양만 대도 주변 잘 피밭에서 채집된 가시망둑의 식성을 조사하였다. 가시망둑의 주요 먹이는 어류, 새우류, 단각류 (옆새우류 및 카프렐라류)였으며, 그 외 주걱벌레붙이류, 요각류, 등각류, 곤쟁이류, 갯지렁이류 등이 소량 섭이되었다. 가시망둑은 초기에 단각류를 많이 섭이하였으나, 성장함에 따라 단각류의 섭이율은 감소하였으며, 그 대신 어류의 섭이율이 점차 증가하였다. 먹이 종류는 계절에 따라 크게 변하지 않았으며, 대체적으로 계절에 관계없이 어류와 새우류가 주로 섭이되었다.

• Journal of Industrial and Engineering Chemistry
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• 제67권
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• pp.293-300
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• 2018

Lipocalins are diverse group of small extracellular proteins found in various organisms. In this study, members of 10 non-homologous lipocalin-ligand crystal complex structures were remodeled using rigid and flexible ligand modes to validate the prediction efficiency of molecular docking simulation. The modeled ligand conformations indicated a high prediction accuracy in rigid ligand mode using cluster based analysis for most cases whereas the flexible ligand mode required further considerations such as ligand binding energy and RMSD for some cases. This in silico study is expected to serve as a platform in the screening of novel ligands against lipocalin family of proteins.

• Advances in nano research
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• 제14권3호
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• pp.273-284
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• 2023

Combating the worldwide environmental threat of plastic waste pollution has become a priority. Plastic pollution has the potential to impact land, rivers, and seas, since many marine and terrestrial organisms have perished as a result of plastic's non-biodegradability and soil dangers. For this consumption, it seems required to manufacture and use new renewable resources. Renewable materials for diverse applications have been created utilizing nanotechnology, which may replace conventional materials for children's activities and sports equipment. This study investigates and suggests that nanotechnology-based materials be replaced with conventional materials to save the environment in manufacturing equipment for children's physical activities. On the basis of the mechanical sciences, a stability study of the bending behavior of small-scale structures will be performed for the various recommended materials.

• 한국안광학회지
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• 제19권1호
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• pp.59-67
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• 2014

목적: 콘택트렌즈 관리용품의 살균력 효능 검사법 기준 개발을 위하여 막여과법(Membrane filtration method)과 희석중화법(Dilution-neutralization method)의 살균력 검사 결과를 비교하고 시판되고 있는 콘택트렌즈 관리용품의 살균력을 제시하고자 하였다. 방법: 포도상구균(Staphylococcus aureus), 녹농균(Pseudomonas aeruginosa), 세라티아균(Serratia marces cens), 칸디다균(Candida albicans)을 대상으로 하여 FDA(Food and Drug Administration)기준인 막여과법과 식품의약품안전처에서 새롭게 가이드라인으로 제시할 희석중화법의 살균력 효능 결과를 비교하였으며 총 20종 다목적 용액의 살균력을 측정하였다. 결과: 살균효과가 강한 살균 성분 및 관리용품의 효능은 막여과법과 희석중화법 모두 동일한 결과를 나타냈다. 살균효과가 약한 관리용품의 경우는 막여과법 검사시에는 균이 뭉쳐있어 정확한 균 수 측정이 불가능하였으나 희석중화법 검사시는 정확한 균 수 측정이 가능해짐을 확인하였다. 또한 시판되고 있는 관리용품 중에는 살균력 기준을 통과하지 못한 제품이 존재하였으며 특히 세라티아균과 칸디다균에 대한 살균력이 약한 경우가 많았다. 결론: 막여과법에 비해 희석중화법이 소량의 샘플로 더 정확하게 균수를 측정하는 것이 가능하며 결과 확인이 빨라 살균력 효능 검사시 유용하게 사용될 수 있음을 알 수 있었다.

• Nuclear Engineering and Technology
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• 제48권1호
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• pp.274-284
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• 2016

Purpose: The purpose of this work is to describe the spectrometric analysis of gaseous cloud formation over reactor mixed uranium-and-plutonium (UP) fuel $(U_{0.8}Pu_{0.2})O_2$ samples heated to a temperature $>2,000^{\circ}C$, and thus forecast and evaluate radiation hazards threatening humans who cope with the consequences of any accident at a fission reactor loaded by UP mixed oxide $(U_{0.8}Pu_{0.2})O_2$, such as a mixture of 80% U and 20% Pu in weight. Materials and methods: The UP nuclear fuel samples were heated up to a temperature of over $2,000^{\circ}C$ in a suitable assembly (apparatus) at out-of-pile experiments' implementation, the experimental in-depth study of metabolism of active materials in living organisms by means of artificial irradiation of pigs by plutonium. Spectrometric measurements were carried out on the different exposed organs and tissues of pigs for the further estimation of human internal exposure by nuclear materials released from the core of a fission reactor fueled with UP mixed oxide. Results: The main results of the research described are the following: (1) following the research on the influence of mixed fuel fission products (radioactive isotopes being formed during reactor operation as a result of nuclear decay of elements included into the fuel composition) on living organisms, the authors determined the quantities of plutonium dioxide ($PuO_2$) that penetrated into blood and lay in the pulmonary region, liver, skeleton and other tissues; and (2) experiments confirmed that the output speed of plutonium out of the basic precipitation locations is very small. On the strength of the experimental evidence, the authors suggest that the biological output of plutonium can be disregarded in the process of evaluation of the internal irradiation doses.

• 한국산학기술학회논문지
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• 제21권4호
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• pp.463-470
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• 2020

본 연구는 중소병원 간호사를 대상으로 다제내성균 감염관리 지식과 교육요구도를 파악하여 중소병원 간호사 맞춤형 감염관리 교육프로그램을 개발하고자 시도되었다. 자료수집은 중소병원에 근무하는 간호사 115명을 대상으로 하였다. 자료수집기간은 2019년 11월 15일~11월 30일까지 구조화된 설문지를 이용하여 시행되었다. 수집된 자료는 IBM SPSS 21.0 프로그램을 이용하여 분석하였으며, 기술통계, t-test, ANOVA를 이용하였다. 연구결과 중소병원 간호사의 86.1%가 다제내성균 환자를 간호한 경험이 있었으며 다제내성균 감염관리 교육을 받은 경우가 67.8%로 나타났다. 다제내성균 감염관리지식은 27점 만점에 25.51점이었으며, 다제내성균 환자가 사용한 의료기기에 대한 소독수준, 선제 격리환자의 격리 해제기준, 코호트 격리에서 환자관리, 개인보호구 탈의시점에서 정답률이 낮았다. 교육요구도는 격리병실배정, 선별검사 대상기준, 유행발생관리가 가장 높았다. 또한 일반적 특성에 따른 교육요구도 차이에서 최종학력과 직급에서 차이가 있었다. 따라서 다제내성균의 원내전파 차단 및 유행발생을 예방하기 위해서 다제내성균 감염관리 교육이 중요하므로 다제내성균 감염관리 교육프로그램 개발시 경력별로 교육 구성의 차별화가 필요하다. 신규간호사와 경력간호사 감염관리교육시 필수적으로 다제내성균 감염관리 교육을 수행하는 것이 필요하다.

• 한국초등과학교육학회지:초등과학교육
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• 제35권1호
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• pp.123-135
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• 2016

Interest is one of the important factors in science education. The purpose of this study was to investigate factors affecting the follow-up interest of elementary school students on topics of 'Small Organisms' World'. The follow-up interests of the students on the topic were grouped into three categories; the developed-expanded-deepened (EDD), the simply expanded and maintained (SEM), and the stoped or decreased (SD) types. Each types had specific distinguishing features. Through the examination of students' responses and the in-depth interviews, the factors affecting on the follow-up interests of the students were analyzed. The factors were classified into two types of the external factor and the internal one. While the external factors were mainly related to the instructional materials used in science class and teaching methods, the internal factors were related to the desire for knowing, prior knowledge, students' experience and attitude toward the topics of science. In relation to the types of the follow-up interests, these factors were affected by different causes. The EDD type was most affected by internal factors, such as desire for knowing and prior knowledge. The SEM type was shown to be most affected by external factors, such as instructional materials. The SD type was mainly influenced by external factors, such as teaching methods. From the analysis of internal factors, it was revealed that the attitude and the prior knowledge about the topic are related with the follow-up interests. There was a positive relationship between the levels of science attitude and science knowledge. The students who had EDD type had a higher level of attitude and knowledge. However, there were some students who had higher level of scientific knowledge with SD type. The results of in-depth interviews showed that they were influenced by negative perceptions about science and stress on their grades. In conclusion, each follow-up interest type were caused by the external factors contained in the processes of the science class and the internal ones associated with the individual features and were influenced by the science class. So, the teacher should help the students be able to have the EDD type of follow-up interest which is persisted even after the completion of the science curriculum. For this, when the teachers design science curriculum and plan lesson, they should consider both internal and external factors significantly influencing the students' follow-up interests.

• Journal of Plant Biotechnology
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• 제46권3호
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• pp.205-216
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• 2019

Since the RNA interference (RNAi) had been discovered in many organisms, small non-coding RNA-mediated gene silencing technology, including RNAi have been widely applied to analysis of gene function, as well as crop improvement. Despite the usefulness of RNAi technology, RNAi transgenic crops have various potential environmental risks, including off-target and non-target effects. In this study, we developed methods that can be effectively applied to environmental risk assessment of RNAi transgenic crops and verified these methods in 35S::dsRNAi_eGFP rice transgenic plant we generated. Off-target genes, which can be non-specifically suppressed by the expression of dsRNAi_eGFP, were predicted by using the published web tool, pssRNAit, and verified by comparing their expressions between wild-type (WT) and 35S::dsRNAi_eGFP transgenic rice. Also, we verified the non-target effects of the 35S:: dsRNAi_eGFP plant by evaluating horizontal and vertical transfer of small interfering RNAs (siRNAs) produced in the 35S::dsRNAi_eGFP plant into neighboring WT rice and rhizosphere microorganisms, respectively. Our results suggested that the methods we developed, could be widely applied to various RNAi transgenic crops for their environmental risk assessment.