• 한국어류학회지
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• 제24권3호
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• pp.234-241
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• 2012

좀수수치 Kichulchoia brevifasciata는 남해안 일대의 고흥반도와 거금도, 금오도 일대에 매우 제한적으로 서식하는 한국고유종 어류이다. 개체군의 크기가 급격하게 감소함에 따라 좀수수치의 분포 및 서식지 특성에 관한 정밀한 연구의 필요성이 대두되었다. 따라서 본 연구에서 기존 분포지역을 포함한 21개 지점을 조사하여 5개 지점에서 좀수수치의 서식을 확인하였다. 좀수수치의 서식이 확인된 지점을 서식지의 환경조건과 좀수수치의 관찰 및 채집된 개체수를 기준으로 서식조건이 양호한 지역, 서식처 보존이 요구되어지는 지역, 서식지의 훼손으로 서식위협을 받고 있는 지역으로 구분하였다.

• Animal cells and systems
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• 제16권3호
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• pp.224-229
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• 2012

The Korean shiner (Coreoleuciscus splendidus, Gobioninae), a small freshwater fish native to Korea Peninsula, widely inhabits in most of the major drainages in South Korea. Here we describe the development of 14 novel and polymorphic microsatellites for this species and its effective utilization in estimation of genetic diversity using 72 individuals from three sampling sites in different drainages. Loci were isolated from a microsatellite enrichment procedure using probe-labeled magnetic beads. A total of 242 alleles were detected across all loci with an average of 17.3 alleles per locus ranging 4 32. The loci varied levels of polymorphism as evident from its expecte heterozygosity ranging from 0.111 to 0.957. The average pairwise $F_{ST}$ between two populations examined shows significant differentiation ($F_{ST}$ = 0.215, p<0.05). The 14 microsatellite loci developed here will also be useful to explain for the genetic structures among geographically isolated populations and gene flow dynamics within drainages in this species as well as the closely related species.

• Parasites, Hosts and Diseases
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• 제42권3호
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• pp.93-119
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• 2004

Acanthamoeba and Naegleria are widely distributed in fresh water, soil and dust throughout the world, and cause meningoencephalitis or keratoconjunctivitis in humans and other mammals. Korean isolates, namely, Naegleria sp. YM-1 and Acanthamoeba sp. YM-2, YM-3, YM-4, YM-5, YM-6 and YM-7, were collected from sewage, water puddles, a storage reservoir, the gills of a fresh water fish, and by corneal washing. These isolates were categorized into three groups based on the mortalities of infected mice namely, highly virulent (YM-4), moderately virulent (YM-2, YM-5 and YM-7) and nonpathogenic (YM-3). In addition, a new species of Acanthamoeba was isolated from a freshwater fish in Korea and tentatively named Korean isolate YM-4. The morphologic characters of its cysts were similar to those of A. culbertsoni and A. royreba, which were previously designated as Acanthamoeba group III. Based on experimentally infected mouse mortality, Acanthamoeba YM-4 was highly virulent. The isoenzymes profile of Acanthamoeba YM-4 was similar to that of A. royreba. Moreover, an anti-Acanthamoeba YM-4 monoclonal anti-body reacted only with Acanthamoeba YM-4, and not with A. culbertsoni. Random amplified polymorphic DNA marker analysis and RFLP analysis of mitochondrial DNA and of a 188 small subunit ribosomal RNA, placed Acanthamoeba YM-4 in a separate cluster based on phylogenic distances. Thus Acanthamoeba YM-4 was identified as a new species, and assigned Acanthamoeba sohi. Up to the year 2002 in Korea, two clinical cases were found to be infected with Acanthamoeba spp. These patients died of meningoencephalitis. In addition, one case of Acanthamoeba pneumonia with an immunodeficient status was reported and Acanthamoeba was detected in several cases of chronic relapsing corneal ulcer, chronic conjunctivitis, and keratitis.

• Fisheries and Aquatic Sciences
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• 제27권3호
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• pp.145-158
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• 2024

Life history characteristics, habitat landscape, and historical events are believed to have shaped the patterns of genetic variation in many taxa. The bony lip barb, Osteohilus vittatus, represent a potamodromous fish that complete all life cycle in freshwater and is widely distributed in Southeast Asia. It usually lives in small rivers and other freshwater habitats, and movement between habitats for either food or reproduction has been typical. These life history characteristics may promote gene flow, leading to less structured populations. However, many freshwater habitats are fragmented, which restricts gene flow. We investigate how this interplay has shaped patterns of genetic variation and phylogeographic structure within this species in the Sundaland, a biodiversity hotspot with a complex geological history, using mitochondrial cytochrome oxidase I (mtCOI) as a genetic marker. Forty-six mtCOI sequences of 506 bp long were collected from ten localities, eight geographically isolated and two connected. The sequences were used for population genetic and phylogeographic analyses. Our results showed a low genetic diversity within populations but high between populations. There was a deep phylogeographic structure among geographically isolated populations but a lack of such structure in the connected habitats. Among geographically isolated populations, sequence divergence was revealed, ranging from 1.8% between Java and Sumatra populations to 12.2% between Malaysia and Vietnam. An indication of structuring was also observed among localities that are geographically closer but without connectivity. We conclude that despite high dispersal capacity, the joint effects of historical events, long-term geographic isolation associated with sea level oscillation during the Pleistocene, and restricted gene flow related to lack of habitat connectivity have shaped the phylogeographic structure within the O. vittatus over the Sundaland.

• 한국양식학회지
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• 제9권3호
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• pp.187-194
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• 1996

어류 종묘생산시 중요한 먹이생물로 사용되는 한국산 rotifer를 순수 분리하기 위하여 염전, 기수호, 하구 및 어류 양식장을 중심으로 채집하였다. 해수산 rotifer, B. plicatilis는 13 지역에서 L-type rotifer 2 strains과 S-type rotifer 14 strains을 순수 분리하였다. 담수산 rotifer, B. calyciflorus는 4곳의 양식장에서 4 strains을 순수 분리하였다. 순수 분리된 해수산 rotifer, B. plicatilis. L-type과 S-type rotifer strain의 피갑장과 내구란 장경의 분포는 각각 $244.3\~255.3\;{\mu}m,\;142.4\~145.5\;{\mu}m$와 $131.0\~165.8\;{\mu}m,\;93.7\~116.4\;{\mu}m$로 나타났다. 또 담수산 rotifer, B. calyciflorus strains의 피갑장과 내구란 장경의 분포는 각각 $211.8\~229.9\;{mu}m$와 $126.8\~140.2\;{\mu}m$로 나타났다. B. plicatilis는 성장과 내구란 생산이 매우 다양하게 나타났다. L-type과 S-type의 rotifer의 최고 밀도는 각각 O-L이 200개체/ml였고, H-S가 753.3개체/ml로 가장 높게 나타났다. 생산된 내구란은 S-type에서는 YY-S가 86.7개/ml로 가장 높았고, L-type에서는 O-L이 45.8개/ml로 높았다.

• 한국어류학회지
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• 제21권4호
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• pp.262-272
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• 2009

실뱀장어용 생태 어도를 설계하기 위하여 2008년 실뱀장어 소상 시기인 3~5월에 국립수산과학원 어군행동실험실에 실뱀장어가 소상하는 하구 및 하구둑과 어도를 재현하는 모형 어도를 제작하여 다양한 소상 환경과 어도 조건에서 실뱀장어의 소상행동을 관찰 분석하였다. 아래 해수조에 순치한 실뱀장어를 두고 위 담수조의 담수를 아래로 흐르게 하면서 해수조에서 어도를 통해 담수조로 소상한 실뱀장어의 개체수를 비교하고, 실뱀장어의 소상 행동을 관찰하여 최적 소상을 위한 적정 어도 조건을 추정하였다. 실뱀장어는 수돗물로는 소상하지 않았고, 저수지물보다 강물을 선호하였다. 실뱀장어 소상량은 소조기보다 대조기에 유의하게 많았고(p<0.05), 낮보다 밤에 유의하게 많았다(p<0.05). 실뱀장어 소상량은 저조위보다 고조위에서 유의하게 많았다 (p<0.05). 실뱀장어는 어도 경사면 길이가 짧을수록 소상량이 많았고, 경사도는 $30^{\circ}$ 이하가 효율적이었다 (p<0.05). 유속은 0.4 m/sec 이하로 물이 약하게 흐를 때 소상량이 많았다(p<0.05). 어도의 바닥은 솔, 자갈 및 카펫 등과 같이 거친 재질에서 소상량이 유의하게 많았다(p<0.05).

• 한국어류학회지
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• 제27권1호
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• pp.50-54
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• 2015

2013년 제주도 남부 연안으로 유입되는 소하천에서 구굴무치과(Eleotridae) 구굴무치속(Eleotris)에 속하는 어류 4개체(표준체장 50.9~69.0 mm)를 채집하였다. 이들은 등지느러미가 2개이며 배지느러미가 서로 유합하지 않고 분리되어 있는 점, 새개부 상하 공기열이 새개부 후단에서 서로 만나지 않는 점, 협부 4번째 공기열이 13번째 공기열을 지나 하방으로 신장하는 점 등에서 E. acanthopoma로 동정되었다. 본 종은 이러한 특징에서 북부 서태평양 해역에 분포하는 구굴무치를 포함한 같은 속의 유사종과 쉽게 구별되며, 신한국명으로는 체색이 대체로 흑색인 점에서 '검은구굴무치'를 제안한다.

• 한국농공학회논문집
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• 제46권3호
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• pp.93-105
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• 2004

The Shihwa wetland, a newly developed constructed wetland prototype in Korea with an area of approximately 104 ha, was established to improve the water quality severely polluted inflow streams into Lake Shihwa. Because this wetland could play a role as an ecological park in addition to improving the water quality, an ecological impact of the Shihwa constructed wetland has been a national concern. This paper focused on reporting the survey results for fauna among the entire investigation results for 3 years. A total of 129 terrestrial insect species have been observed from August 2001 to June 2002. Among them, Ischnura asiatica (Brauer) (order Odonata), Scymnus species (order Coleoptera) and Orthopteran species were frequently found in the reed bushes. A total of 77 bird species were recorded in a seasonal count, the maximum number of species was 34 in winter and the maximum number of individuals was 4,599 in summer. For the freshwater fish, only 4 species were found in 2000, however in 2001 and 2002, 12 species and 459 individuals were collected at four survey points. Among these 12 species, the dominant species were Mugil cephalus(36%), followed by Carassius auratus (25%) and Rhinogobius brunneus (22%). Meanwhile,12 individuals of Oryzias latipes were observed nearby, mostly downstream of the wetland. For the Herpetofauna at four survey areas, 3 species of amphibians and 3 species of reptiles were recorded. Because of remaining salinity in the soil of the Shiwha constructed wetland, Herpetofauna inflow to the wetland was scanty and mainly inhabited the upstream area. A total of 8 mammal species were recorded. Small-sized species were the striped field mouse, the Ussurian harvest-mouse, the Manchurian reed vole and the brown rat. Middle- and large-sized species were Korean water-deer, Korean raccoon dogs, Korean yellow weasels and feral cats.

• 한국수산과학회지
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• 제56권6호
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• pp.766-772
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• 2023

This study was conducted to compare the nutritional properties of cookies made with Israeli carp Cyprinus carpio (C-IC) to those made without Israeli carp (control). The proximate composition of C-IC per 100 g was 4.1 g moisture, 9.7 g protein, 29.2 g lipid, 1.4 g ash, and 55.6 g carbohydrates. Moisture, protein and ash contents were significantly higher and the carbohydrate content was significantly lower (P<0.05) in C-IC than control, but there was no difference in lipid content (P>0.05). The total amino acid content of C-IC per 100 g was 9.46 g and the major amino acid was glutamic acid (2.49 g). The mineral contents of C-IC per 100 g were 216.6 mg calcium, 193.2 mg phosphorus, 170.9 mg potassium, and 18.2 mg magnesium, which were all significantly higher than the contents of the control (P<0.05). The major fatty acids of C-IC were 16:0, 18:1n-9, and 18:2n-6. The digestibility of C-IC in the small intestine was 51.3%, which was higher than the digestibility of the control. These results suggest that C-IC have better nutritional properties than the control.

• 한국식품위생안전성학회지
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• 제29권4호
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• pp.347-355
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• 2014

본 연구에서는 식품 중 동물성 사용원료의 진위 판별을 위하여 분자생물학적 기법을 이용한 시험법을 개발하였다. 동물성 식품원료의 종 판별을 위한 유전자로는 미토콘드리아 DNA에 존재하는 COI, Cytb, 및 16S rRNA 유전자를 대상으로 하였으며, 가공식품에 적용하기 위하여 PCR 산물의 크기는 200 bp 내외가 되도록 종 특이 프라이머를 설계하였다. 대상종으로는 가축류 2종, 가금류 6종, 민물어류 2종, 해양어류 13종 및 갑각류 1종, 총 24종을 선정하였으며 종 특이 프라이머를 이용하여 예상되는 PCR 산물의 생성 유무를 확인하였다. PCR을 수행한 결과 토끼, 여우, 꿩, 집비둘기, 멧비둘기, 메추리, 참새, 제비, 메기, 쏘가리, 날치, 열빙어, 청어, 까나리, 멸치, 참조기, 넙치, 조피볼락, 홍어, 가오리, 말쥐치, 농어, 성게 및 바닷가재에 대하여 각각 156, 204, 152, 160, 113, 163, 167, 152, 165, 121, 136, 151, 178, 178, 146, 188, 177, 166, 179, 218, 188, 185, 127 및 172 bp에서 PCR 증폭 산물을 확인하였다. 그리고 프라이머 별로 비교종에서는 비특이적 PCR 산물(non-specific PCR product)은 생성되지 않았다. 본 연구에서 개발된 유전자 분석법을 이용하여 동물성 식품원료가 사용된 식품 원료 및 가공식품의 진위 판별에 활용이 가능할 것이며, 불량식품 근절에 크게 기여할 것으로 기대된다.