• Journal of Life Science
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• v.5 no.1
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• pp.8-19
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• 1995

The Optimal condition for degradation of crystal violet and other triphenylmethane dyes by Citrobacter sp. SK-3 isolated from the activated sludge of dye manufacturing factory was investigated. The optimal culture medium for the degradation of triphenylmethane dye was composed of minimum inorganic salt medium supplemented with 0.5% galactose, 0.1% beef extract, with the initial pH of 8.0 to 9.0. Under this condition, Citrobacter sp. SK-3 degraded 200 ppm of crystal violet completely within 24 hours. Citrobactre sp. SK-3 also degraded efficiently malachite green, pararosaniline, brilliant green, methyl violet, basic fuchsin and methyl red. Analysis of the degradation products of crystal violet through this layer chromatography and high performance liquid chromatography indicated that the methyl groups bound to crystal violet backborn were gradually demethylated to pentamethyl-, tetramethyl- and trimethylpararosaniline.

• Korean Journal of Microbiology
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• v.44 no.1
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• pp.22-28
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• 2008

Cutting oils are emulsionable fluids widely used in metal working processes. Their composition is mineral oil, water, and additives (fatty acids, surfactants, biocides, etc.) generating a toxic waste after a long use. Cutting oils also affect colour, taste and odour of water, making it undesirable for domestic and industrial uses. In these days, conventional treatment methods as evaporation, membrane separation or chemical separation have major disadvantages since they generate a concentrated stream that is more harmful than the original waste. In this study, our purpose is to reduce cutting oils by using biological treatment. Eighty one strains were isolated from cutting waste oil of industrial waste water sludge under aerobic conditions. Among these strains, KS47, which removed 90.4% cutting oil in 48 hr, was obtained by screening test under aerobic conditions(pH 7, $28^{\circ}C$). KS47 was identified as Pseudomonas aeruginosa according to morphological, physiological and biochemical properties, 16S rDNA sequence, and fatty acid analysis. P. aeruginosa KS47 could utilize cutting oil as carbon source. In batch test, we obtained optimal degradation conditions(1.5 g/L cell concentration, pH 7, and temperature $30^{\circ}C$). Under the optimal conditions, 1,060 mg/L cutting oil was removed 83.7% (74.1 mg/L/hr).

• Journal of Korea Soil Environment Society
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• v.3 no.2
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• pp.115-125
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• 1998

The biodegradation of ($C^{14}$)phenanthrene was studied in water and soil-water systems with nonionic surfactants and biosurfactant : polyoxyethylene alkyl ester($C_{17}$$H_33$COO($C_2$$H_4$O)nH) and sophorolipid. The extents of so1ubilization and biodegradation were monitored by radiotracer technique. Experimental results showed that surfactant concentrations above the critical micelle concentration were toxic to the phenanthrene-degrading bacteria in soil or active sludge and the presence of surfactant micelles inhibited mineralization of PAHs. Solubility and bioavailibility of phenanthrene in water and soil-water system were enhanced by mixed surfactants system. The optimum water content and hydrogen concentration were 30% (w/v) , pH 7, respectively.

• Journal of Environmental Science International
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• v.23 no.1
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• pp.129-142
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• 2014

To treat chromaticity contained in effluents of dyeing wastewater efficiently, potent dye-degrading microorganisms were isolated from influent water, aeration- tank sludge, recycle water and settling-tank sludge located in leather and dyeing treatment plant. Six potent strains were finally isolated and identified as Comamonas testosteroni, Methylobacteriaceae bacterium, Stenotrophomonas sp., Kluyveromyces fragilis, Ascomycetes sp. and Basidiomycetes sp. When Basidiomycetes sp. was inoculated into ME medium containing basal mixed-dyes, 93% of color was removed after 8 days incubation. In the same experiment, the 1:1 mixed culture of Basidiomycetes sp. and photosynthetic bacterium exhibited 88% of color removal; however, it showed better color removal for single-color dyes. The aeration-tank and settling-tank samples revealed higher color removal (95-96%) for black dyes. The settling-tank sample also revealed higher color removal on basal mixed-dyes, which resulted in 90% color removal after 6-h incubation. From the above results, it is expected to achieve a higher color removal using the mixed microorganisms that were isolated from aeration-tank and settling-tank samples.

• KSBB Journal
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• v.22 no.3
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• pp.157-161
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• 2007

This study is to investigate the biological degradation and the characteristics of MEA, a pH regulator to be put in the cooling water circulation system for power plants, loading to elevate concentrations of COD and N when eluted into the water environment. MEA, $NH_4^+$ and CODcr were monitored in flask cultures and in a batch aerator. MEA was found to be biologically degradable, producing substantial amount of ammonia (max. 78.1%) in a form of $NH_4^+$ and other carboneous intermediates. The degradation reaction rates were similar one another over all MEA concentrations tested as the activated sludge (microbial consortium) was acclimated to MEA with the gradual and stepwise increase in MEA input into the batch aerator. Also, MLVSS kept increasing with increasing MEA input. The COD-based degradation reaction order was determined to be 1.

• Journal of Korean Society on Water Environment
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• v.33 no.6
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• pp.754-768
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• 2017

Polybrominated diphenyl ethers (PBDEs) are a group of industrial aromatic organobromine chemicals that have been used since the 1970s as flame retardants in a wide range of consumer products and articles, including plastics, computers, textiles and upholstery. Commercial PBDEs were added to Annex A of the Stockholm Convention as persistent organic pollutants in May 2009. PBDEs are still frequently found in sludge and effluent from wastewater treatment plants, even though commercial PBDEs were prohibited or voluntarily phased out several years ago. Conventional wastewater treatment processes are not designed to effectively remove PBDEs. This indicates that there is an urgent need for new developments and improvements to enhance upon the treatment techniques which are currently available. Several studies have suggested the potential removal and degradation technologies for PBDEs in wastewater. In this study, the concentrations and compositional profiles of PBDE congeners in sludge and effluent are investigated by analyzing the relevant literature data in relation to their usage patterns in commercial products in North America and South Korea. The strengths and weaknesses of the current PBDEs removal techniques (i.e., biodegradation, zero-valent iron, photolysis, sorption, etc.) are discussed critically. In addition, future research direction regarding the treatment and removal of PBDEs from wastewater is also suggested, based on the literature review.

• KSBB Journal
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• v.14 no.6
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• pp.688-695
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• 1999

Biodegradation of the reactive dye, Remazol Black B was investigated in an upflow anaerobic sludge blanket(UASB) reactor. Important parameters studied include dye concentration(20-60 mg/L), glucose concentration as a co-substrate(1,000-3,000 mg/L), hydraulic retention time(3-24 hr), and influent pH(6.0-8.0). Under most conditions tested, the molecules of Black B were degraded readily and completely according to HPLC chromatograms. However, the color removal efficiency based on spectroscopic measurement was always approximately 75%. This suggests that the degradation products have some color intensity corresponding to 25% of the original dye molecules. The maximum influent dye concentration which satisfies the legal discharge limit of color intensity of 400 ADMI was 13 mg/L. and the highest removal rate at this dye concentration was 104 mg/L${\cdot}$day.

• Journal of Korean Society of Environmental Engineers
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• v.22 no.4
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• pp.619-627
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• 2000

Biodegradation of the C. I. Reactive Blue 114 was investigated in an upflow anaerobic sludge blanket (UASB) reactor. Important parameters studied include dye concentration, the kind and concentration of carbon source, hydraulic retention time (HRT), and influent pH. Glucose was found to be a better co-substrate than the mixture of volatile fatty acids (VFAs), although its concentration did not affect dye removal efficiency in the range of $1000{\sim}3000mg/{\ell}$. When HRT increased from 6 hr to 24 hr, dye removal efficiency increased up to 12 hr and remained almost constant thereafter at about 40%. When influent pH was varied in the range of 6.0~8.0, the effluent pH was varied in the range of 6.8~7.5 with maximum efficiency at pH 7.0. The highest dye removal rate obtained was $52mg/{\ell}{\cdot}day$, while the maximum dye load to meet the discharge limit of color intensity was estimated to be $46mg/{\ell}{\cdot}day$ at HRT of 12 hr and an influent glucose concentration of $2200mg/{\ell}$.

• Journal of Korean Society of Environmental Engineers
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• v.30 no.4
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• pp.393-400
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• 2008

1,4-Dioxane($C_4H_8O_2$), which is used as a solvent stabilizer, could make harmful effects on ecosystem because of its higher solubility, toxicity and carcinogenic by US EPA. From 2011, its discharge limit to waterbody will be regulated at 5 mg/L by Ministry of Environment Republic of Korea. It was thus to investigate that the currently operating activated sludge in polyester manufacturing processes in Gumi can properly treat it to meet with the regulation standard. For that purpose, the removal rate of 1,4-dioxane and its microbial properties were assessed for a few companies(i.e. K, H and T). Its removal efficiency was the most highly recorded in H as 98% and then 77% for K, which met with the regulation standard. However, concentration of 1,4-dioxane of T was 23 mg/L in the effluent, which is more than the regulation standard. Aside from, microbial degradation test was done for 100 ppm of 1,4-dioxane in BSM (Basal salt medium) inoculated with each of activated sludge. After 7 days, 1,4-dioxane was completely removed in the test bottle inoculated with H sludge, 67% in T and 52% in K, which could confirm that the given activated sludge might have different biodegradability against the amount of 1,4-dioxane. Therefore, microbial diversity in each company was investigated by 16s rDNA cloning methods where a species, e.g. Methylibium petroleiphilum PM1, was the greatest observed from H and in lesser from K, but it was not detected from T. Methylibium petroleiphilum PM1 is known to efficiently degrade ether like methyl tertiary-butyl ether(MTBE). It is concluded that the activated sludge in H can be most effectively adopted for a biodegradation of 1,4-dioxane in the concern of industrial sector.

• Journal of the Korean Society of Clothing and Textiles
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• v.29 no.3_4 s.141
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• pp.470-477
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• 2005

This study was carried out to evaluate the biodegradability of viscose rayon and lyocell fibers, employing soil burial test, activated sludge test and enzymatic hydrolysis. Using X-ray analysis, crystallinity and morphology change was investigated. External changes after degradation were also observed by SEM and digital photographs. Vscose rayon fibers exhibited higher biodegadation than lyocell fibers, indicating that lower crystallinity favored the biodegradation. Among the biodegradability of lyocell fibers there was a tendency that fibers with lower crystallinity and higher moisture regain had higher values. When external changes after degradation being observed, it was shown that there were microorganisms growing on the surfaces of samples accompanying lading and weakening. From these results it was concluded that biodegradability of the specimens was most closely correlated to the moisture regain and crystallinity of fibers which reflects hydrophilicity and internal structure.