• The Korean Journal of Physiology and Pharmacology
• /
• v.21 no.1
• /
• pp.27-36
• /
• 2017

Angelicae Gigantis Radix (AGR, Angelica gigas) has been used for a long time as a traditional folk medicine in Korea and oriental countries. Decursinol angelate (DCA) is structurally isomeric decursin, one of the major components of AGR. This study was performed to confirm whether DCA augments pentobarbital-induced sleeping behaviors via the activation of $GABA_A$-ergic systems in animals. Oral administration of DCA (10, 25 and 50 mg/kg) markedly suppressed spontaneous locomotor activity. DCA also prolonged sleeping time, and decreased the sleep latency by pentobarbital (42 mg/kg), in a dose-dependent manner, similar to muscimol, both at the hypnotic (42 mg/kg) and sub-hypnotic (28 mg/kg) dosages. Especially, DCA increased the number of sleeping animals in the sub-hypnotic dosage. DCA (50 mg/kg, p.o.) itself modulated sleep architectures; DCA reduced the counts of sleep/wake cycles. At the same time, DCA increased total sleep time, but not non-rapid eye movement (NREM) and rapid eye movement (REM) sleep. In the molecular experiments. DCA (0.001, 0.01 and $0.1{\mu}g/ml$) increased intracellular Cl- influx level in hypothalamic primary cultured neuronal cells of rats. In addition, DCA increased the protein expression of glutamic acid decarboxylase ($GAD_{65/67}$) and $GABA_A$ receptors subtypes. Taken together, these results suggest that DCA potentiates pentobarbital-induced sleeping behaviors through the activation of $GABA_A$-ergic systems, and can be useful in the treatment of insomnia.

• Biomolecules & Therapeutics
• /
• v.16 no.4
• /
• pp.328-335
• /
• 2008

This study was undertaken to investigate whether honokiol could enhance the pentobarbitalinduced sleeping behaviors through $\gamma$-aminobutyric acid (GABA) receptor $Cl^-$ channel activation. Thirty minutes after the oral administration of honokiol, mice were received sodium pentobarbital (42 mg/kg, i.p.). The time elapsed from pentobarbital injection to the loss of the righting reflex was taken as sleeping latency. The time elapsed between the loss and voluntary recovery of the righting reflex was considered as the total sleeping time. Western blot technique and $Cl^-$ sensitive fluorescence probe were used to detect the expression of $GABA_A$ receptor subunits and $Cl^-$ influx in the primary cultured cerebellar granule cells. Honokiol (0.1 and 0.2 mg/kg) prolonged the sleeping time induced by pentobarbital (42 mg/kg) in a dosage-dependent manner. Honokiol (20 and 50 ${\mu}M$) increased $Cl^-$ influx in primary cultured cerebellar granule cells, and selectively increased the $GABA_A$ receptor $\alpha$-subunit expression, but had no effect on the abundance of $\beta$ or $\gamma$-subunits. Chronic treatment with 20 ${\mu}M$ honokiol in primary cultured cerebellar neurons did not affect the abundance of GAD65/67. The results suggested that honokiol could potentiate pentobarbital-induced sleeping through $GABA_A$ receptor $Cl^-$ channel activation.

• The Korean Journal of Pharmacology
• /
• v.6 no.1
• /
• pp.15-22
• /
• 1970

The present study is concerned with the demonstration of the relationship between the behavior and the brain concentration of noradrenaline resulted from pretreatment of amphetamine in isolated or aggregated rats. The experimental subjects were rats weighing from 120g to 200g housed 1, 2, and 6 in a cage. Analeptic activity of amphetamine was measured by determining the sleeping time induced by pentobarbital sod. The noradrenaline content in brain was determined with Aminco-Bowmann's spectro-photofluorometer by Lee's modification of Shore and Olin method. Results: 1) The analeptic activity of amphetamine on the sleeping time induced by pentobarbital sod. was more increased in the grouped rats than in isolated animal. 2) In being isolated and grouped rats, the sleeping time induced pentobarital sod, was markedly prolonged by pretreatment of amphetamine. 3) Means of housing rats, e.g., isolation or aggregation did not seem to affect the brain noradrenaline depleting action. 4) Repeated daily parenteral administration of amphetamine sulfate for a period 1 to 3 weeks resulted in decrement of brain noradrenaline concentration in being isolated and grouped rats. 5) The prolongation of sleeping time of the isolated or aggregated rats, when pretreated with amphetamine, compared with that of stock rats, seems to be attributable rather to the means of housing than the variation of the noradrenaline caused by amphetamine.

• Journal of Acupuncture Research
• /
• v.33 no.3
• /
• pp.181-187
• /
• 2016

Objectives : The purpose of this study is to report the clinical effects of 8 constitution acupuncture on a patient with complex regional pain syndrome (CRPS) type I. Methods : In this study, a patient with CRPS was treated by 8 constitution acupuncture therapy. He was diagnosed in the Cholecystonia of 8 constitution diagnosis. The progress of symptoms were evaluated by visual analog scale (VAS) and sleeping time. VAS and sleeping time were evaluated at 7:00 am daily. Results : VAS decreased from 10 to 4, while sleeping time increased from 30 minutes to 3 hours. Conclusion : The results suggest that 8 constitution acupuncture is effective for treating symptoms of CRPS type I.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.36 no.2B
• /
• pp.111-119
• /
• 2011

As Green-IT has attracted a lot of attention in recent years, many researches have been interested in reducing the energy consumption of network equipments. In this paper, we analyze the energy saving ratio and delay performance according to various traffic patterns and burst assemble algorithms in OBS network with sleeping mode. To do this, we design the traffic generators, which are based on exponential distribution and Pareto distribution, and the router model, which has the time based and length based burst assemble algorithms by using OPNET modeler. Through OPNET simulator, we evaluate the energy saving performance in terms of the sleeping time, the number of transitions and packet delay.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.31 no.12B
• /
• pp.1028-1036
• /
• 2006

Energy conservation is an important issue in wireless networks, especially for self-organized, low power, low data-rate impulse-radio ultra-wideband (IR-UWB) networks, where every node is a battery-driven device. To conserve energy, it is necessary to turn node into sleep state when no data exist. This paper addresses the energy consumption analysis of Direct-Sequence (DS) versus Time-Hopping (TH) multiple accesses and two kinds of sleeping protocols (slotted and unslotted) in PHY-MAC for Un networks. We introduce an analytical model for energy consumption or a node in both TH and DS multiple accesses and evaluate the energy consumption comparison between them and also the performance of the proposed sleeping protocols. Simulation results show that the energy consumption per packet of DS case is less than TH case and for slotted sleeping is less than that of unslotted one for bursty load case, but with respect to the load access delay unslotted one consumes less energy, that maximize node lifetime.

• Child Health Nursing Research
• /
• v.4 no.2
• /
• pp.265-273
• /
• 1998

The purpose of this study was to identify the children's sleeping patterns, such as the sleeping hours and the nature of sleep disruptions following hospitalization and its accompanying factors. The data were collected from December, 1997 to March, 1998 using a questionnaire developed by researchers. The subjects were 76 children in a hospital. The results of this study were summarized as follows : 1. The average sleeping hours (sleep duration) at night were 10 hours and 10 minutes and 9 hours and 9 minutes before and after hospitalization respectively. There was a significant difference (p<0.01). The average sleeping hours in the day time were 1 hour 28 minutes and 2 hours and 26 minutes before and after hospitalization respectively. There was a significant difference(P<0.01). 2. The mean bed time(sleep onset) was 10: 22 pm and 10 : 28 pm before and after hospitalization respectively. There was no significant difference. 3. The mean hour of rising(sleep termination) was 7: 54 am 7 : 08 am before and after hospitalization respectively. There was a significant difference (p<0.01). 4. The mean number of sleep disruption was 0.72 and 1.94 before and after hospitalization respectively. There was a significant difference(P<0.01). The sleep disruptions were influenced by crying of other children(53.9%), lights(28.9), nursing procedures(18.4%), noise of TV(17.l%) and noise of visitors (15.8%).

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.32 no.6B
• /
• pp.316-324
• /
• 2007

In this paper we proposed an energy consumption model for IR-UWB wireless sensor networks. The model takes the advantages of PHY-MAC cross layer design, and we used slotted and un-slotted sleeping protocols to compare the energy consumption. We addressed different system design issues that are responsible to energy consumption and proposed an optimum model for the system design. We expect the slotted sleeping will consume less energy for bursty load than that of the un-slotted one. But if we consider latency, the un-slotted sleeping model performs better than the slotted sleeping case.

• Archives of Pharmacal Research
• /
• v.8 no.3
• /
• pp.119-132
• /
• 1985

The apparent effectiveness of 1-naphthyl-N-methyl carbamate (carbaryl) against a wide variety of insects motivated the study of its mammalian toxicity. In this toxicological study of carbaryl, mature male rats inhaled carbaryl at a mean concentration of 112mg, 168mg and 224 mg/$m^{3}$ for one hour. After inhalation, pentobarbital sleeping time, Nadph-cytochrome c reductase activity, cytochrome p-450 and protein content in liver microsomes, various tissue residues, cholinesterase inhibition in plasma and histopathological findings at autopsy were observed. The pentobarbital sleeping time was prolonged in rats inhaled with carbaryl for one day while the sleeping time was shortened in the 3 days inhaled group. The changes of cytochrome p-450 content and NADPH-cytochrome c reductase activity exhibited biphasic response showing the decrease in the one day inhaled group and the increase in the 3 days inhaled group. The marked depression of plasma ChE activity was observed in rats inhaled with carbaryl at 112 mg/$m^{3}$, however no more progressive effect was observed at the higher concentration of the compound. The main observations in histopathological finding were ciliary detachment, epithelial swelling and subepithelial inflammatory cellular infiltration in trachea due to the irritation.

• Natural Product Sciences
• /
• v.19 no.2
• /
• pp.179-185
• /
• 2013

Polygalae radix (PR) has traditionally been used as a sedative and anti-stress agent in oriental countries for a long time. PR which contains many ingredients is especially rich in saponins. This study was performed to investigate whether ethanol extract of PR enhances pentobarbital-induced sleep behaviors. In addition, possible mechanisms also were investigated. PR inhibited locomotor activity in mice. PR increased sleep rate and sleep time by concomitant administration with sub-hypnotic dose of pentobarbital (28 mg/kg). PR prolonged total sleeping time, and shortened sleep latency induced by pentobarbital (42 mg/kg). In addition, PR increased intracellular chloride concentration in primary cultured neuronal cells. The expression level of glutamic acid decarboxylase (GAD) were increased, and ${\gamma}$-aminobutyric acid $(GABA)_A$ receptors subunits were modulated by PR, especially increasing ${\gamma}$-subunit expression. In conclusion, PR augments penobarbital-induced sleep behaviors through activation of $GABA_A$ receptors and chloride channel complex.