• Laboraroty Animal Research
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• 제34권4호
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• pp.279-287
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• 2018

Placenta specific 8 (PLAC8, also known as ONZIN) is a multi-functional protein that is highly expressed in the intestine, lung, spleen, and innate immune cells, and is involved in various diseases, including cancers, obesity, and innate immune deficiency. Here, we generated a Plac8 knockout mouse using the CRISPR/Cas9 system. The Cas9 mRNA and two single guide RNAs targeting a region near the translation start codon at Plac8 exon 2 were microinjected into mouse zygotes. This successfully eliminated the conventional translation start site, as confirmed by Sanger sequencing and PCR genotyping analysis. Unlike the previous Plac8 deficient models displaying increased adipose tissue and body weights, our male Plac8 knockout mice showed rather lower body weight than sex-matched littermate controls, though the only difference between these two mouse models is genetic context. Differently from the previously constructed embryonic stem cell-derived Plac8 knockout mouse that contains a neomycin resistance cassette, this knockout mouse model is free from a negative selection marker or other external insertions, which will be useful in future studies aimed at elucidating the multi-functional and physiological roles of PLAC8 in various diseases, without interference from exogenous foreign DNA.

• 한국수소및신에너지학회논문집
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• 제31권6호
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• pp.605-613
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• 2020

In July and October of this year, the government announced the 'Green new deal plan within the Korean new deal policy' and 'Strategies for proliferation of future vehicles and market preoccupation'. And, in response to changes in the global climate agreement, it has decided to expand green mobility such as electric vehicles and hydrogen electric vehicles with the aim of a "net-zero" society. Accordingly, the goal is to build 310 hydrogen refueling stations along with the supply of 60,000 hydrogen vehicles in 2022, and the hydrogen infrastructure is being expanded. however, it is difficult to secure hydrogen infrastructure due to expensive construction costs and difficulty the selection of a site. In Korea, it is possible to build a mobile hydrogen station according to the safety standards covering special case of the Ministry of Industry. Since the mobile hydrogen station can be charged while moving between authorized place, it has the advantage of being able to meet a large number of demands with only one hydrogen refueling station, so it is proposed as a model suitable for the early market of hydrogen infrastructure. This study demonstrates the establishment of a hydrogen refueling station by deriving a virtual accident scenario for leakage and catastrupture for each facility for the risk factors in a mobile hydrogen station, and performing a quantitative risk assessment through the derived scenario. Through the virtual accident scenario, direction of demonstration and implications for the construction of a mobile hydrogen refueling station were derived.

• 영어영문학
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• 제54권4호
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• pp.561-577
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• 2008

L.M. Montgomery's Anne of Green Gables is not only confidently labelled a Canadian classic but also placed as a national icon along with the moose, the beaver, and the Habs in Canada. Anne's 'Canadianness' is partly due to its location in the rural world of Prince Edward Island. The fictional Avonlea is described as the ideal space where Canadian spirit can interact with the personified surrounding landscapes through Celtic imagination. Additionally, the communal bond of Avonlea fully demonstrates Scottish Canadian identities. The Scottish national character of Avonlea is responsible for clannishness of the Cuthberts and the Lyndes. The disrespect to the French is also due to Scottish heritage in Avonlea. As an outsider Anne wants to be integrated into the community of Avonlea, and successfully adapts herself to the regional shared values. Meanwhile she partly challenges the strictness and rigidness of the born Canadian Avonlea residents. Despite its Canadian origin, Anne of Green Gables is accepted as part of the American canon of children's literature in the Unite States. The configuration of Anne as an American heroine is noticeable among American scholars: by relocating it to the US the female Bildungsroman in the nineteenth century America, a group of literary critics adapt Anne as an American girl for American readers. The heroine of Anne of Green Gables is linked to American novels such as Louisa May Alcott's Little Women, Kate Douglas Wiggin's Rebecca of Sunnybrook Farm and Gene Stratten Porter's A Girl of the Limberlost. Anne is even classified as another Caddie by American literary critics: Anne is placed at the center of Caddie Woodlawn Syndrome as another Wisconsin pioneer child. Canadian identity of Anne is intentionally excluded and Anne was reborn as an American girl in the U.S. In this context, Anne functions as a sign of nation and a site for cross-national identity formation.

• 한국항행학회논문지
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• 제25권3호
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• pp.217-222
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• 2021

EOTS(electro-optical tracking system)는 유도무기의 성능 평가를 위해 유도무기를 추적하여 영상을 획득하는데 활용되고 있다. 유도무기에 대한 추적을 잃어버렸을 경우 유도무기가 매우 빠르게 비행하기 때문에 운용자가 이를 다시 포착하는 것은 거의 불가능하다. 레이더나 텔레메트리 데이터를 활용하여 재 포착 하는 방법이 활용되고 있으나 데이터를 실시간으로 수신할 수 있는 통신망의 설치가 수반되어야하기 때문에 장소에 대한 제약이 따른다. 하지만 유도무기 비행시험 수행 시 계산되는 예상 궤적은 실시간으로 수신할 필요 없이 저장해두었다가 사용할 수 있기 때문에 통신망 설비와 관계없이 활용이 가능하다. 본 논문에서는 미리 알고 있는 비행체의 예상 궤적을 활용하여 비행체를 잃어버렸을 시 비행체의 위치를 예상하는 방법을 제안한다. DTW (dynamic time warping)를 통해 예상궤적과 추적궤적을 비교하여 비행체의 각속도를 추정하고 이를 Kalman Filter의 보정단계에서 관측 값으로 활용하여 비행체의 다음 상태를 예측한다. 제안한 방법의 타당성을 실제 비행체 궤적에 적용하여 검증하였다.

• 한국초지조사료학회지
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• 제40권4호
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• pp.196-202
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• 2020

본 연구는 동계사료작물의 무인기기반 생육모니터링을 위하여 호밀, 총체보리, IRG를 대상으로 다중분광영상으로 건물수량을 예측하기 위한 최적식생지수를 테스트하였다. 2019년 2월부터 4월까지 나주의 실경작지에서 무인기 다중분광카메라로 분광영상을 수집하여 4종류의 식생지수(Normalized Difference Vegetation Index; NDVI, Green Normalized Difference Vegetation Index; GNDVI, Normalized Green Red Difference Index; NGRDI and Normalized Difference Red Edge Index; NDREI)를 산출하고 지상에서 건물수량을 조사하여 식생지수와 건물수량의 상관관계를 조사하였다. 호밀, 총체보리, IRG에 대하여 건물수량과 NDVI의 상관관계(R2)는 0.91~0.92, GNDVI는 0.92~0.94, NGRDI는 0.71~0.85, NDREI는 0.84~0.91로 GNDVI가 가장 효과적이었다.

• 산업경영시스템학회지
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• 제44권4호
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• pp.65-75
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• 2021

This study explores multiple variables of an OTT service for discovering hidden relationship between rating and the other variables of each successful and failed content, respectively. In order to extract key variables that are strongly correlated to the rating across the contents, this work analyzes 170 Netflix original dramas and 419 movies. These contents are classified as success and failure by using the rating site IMDb, respectively. The correlation between the contents, which are classified via rating, and variables such as violence, lewdness and running time are analyzed to determine whether a certain variable appears or not in each successful and failure content. This study employs a regression analysis to discover correlations across the variables as a main analysis method. Since the correlation between independent variables should be low, check multicollinearity and select the variable. Cook's distance is used to detect and remove outliers. To improve the accuracy of the model, a variable selection based on AIC(Akaike Information Criterion) is performed. Finally, the basic assumptions of regression analysis are identified by residual diagnosis and Dubin Watson test. According to the whole analysis process, it is concluded that the more director awards exist and the less immatatable tend to be successful in movies. On the contrary, lower fear tend to be failure in movies. In case of dramas, there are close correlations between failure dramas and lower violence, higher fear, higher drugs.

• Architectural research
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• 제24권2호
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• pp.21-27
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• 2022

For the past decade, South Korea had experienced catastrophic building fires, which resulted in consider-ably high number of casualties. This motivated research to develop fire-safe wall assemblies. In this study Fire Risk Analysis (FRA) is conducted as part of the project designing phase to ensure fire safety of the final product. Traditional approach was to consider fire performance at the end of the designing stage, when PASS/FAIL fire test results are required to be submitted to the Authority Having Jurisdiction (AHJ). By applying a fire risk analysis to guide the designing phase, overall fire safety of a wall assembly can be achieved more systematically as conducting FRA allows designers to clearly identify elements that are more vulnerable to fire and simply replace them with other practical options. Severity of fire risk is determined by considering the fire hazards of a wall assembly such as the exterior layer, insulation, vertical connectivity, and external ignition sources (e.g., photovoltaic panels). Frequency of fire risk is assessed based on the factors affecting fire likelihood, which are air cavity and fire-stopping applied in the design, and random design changes occurring during on-site construction. Fire risk matrix is proposed based on these fire risk factors and efforts to reduce the fire risk level associated with the wall assembly are given by systematically assessing the fire risk factors identified from fire risk analysis. Current study demonstrates how fire risk analysis can be applied to develop fire-safe walls by reducing the relevant fire risks- both severity and frequency.

• Archives of Plastic Surgery
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• 제49권2호
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• pp.227-239
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• 2022

Complications experienced during lymphatic surgery have not been ubiquitously reported, and little has been described regarding how to prevent them. We present a review of complications reported during the surgical management of lymphedema and our experience with technical considerations to reduce morbidity from lymphatic surgery. A comprehensive search across different databases was conducted through November 2020. Based on the complications identified, we discussed the best approach for reducing the incidence of complications during lymphatic surgery based on our experience. The most common complications reported following lymphovenous anastomosis were re-exploration of the anastomosis, venous reflux, and surgical site infection. The most common complications using groin vascularized lymph node transfer (VLNT), submental VLNT, lateral thoracic VLNT, and supraclavicular VLNT included delayed wound healing, seroma and hematoma formation, lymphatic fluid leakage, iatrogenic lymphedema, soft-tissue infection, venous congestion, marginal nerve pseudoparalysis, and partial flap loss. Regarding intra-abdominal lymph node flaps, incisional hernia, hematoma, lymphatic fluid leakage, and postoperative ileus were commonly reported. Following suction-assisted lipectomy, significant blood loss and transient paresthesia were frequently reported. The reported complications of excisional procedures included soft-tissue infections, seroma and hematoma formation, skin-graft loss, significant blood loss, and minor skin flap necrosis. Evidently, lymphedema continues to represent a challenging condition; however, thorough patient selection, compliance with physiotherapy, and an experienced surgeon with adequate understanding of the lymphatic system can help maximize the safety of lymphatic surgery.

• 대한토목학회논문집
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• 제42권1호
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• pp.145-150
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• 2022

도시재생사업을 통해 도시의 쇠퇴에 대응하고자 하는 움직임이 발생하고 있으며 특히 주민주도의 중요성이 증가하고 있다. 이와 더불어 불확실한 미래에 대응하여 도시의 회복력을 높이고자 하는 레질리언스의 개념이 전 세계적으로 대두되고 있다. 본 연구는 레질리언스적 관점에서 도시재생사업을 진단하고자 한다. 연구의 대상지는 서울시의 일반근린형 도시재생사업 선정지역으로 설정하고, 최근 발생한 코로나 바이러스에 대응하여 회복력에 영향을 미치는 요인들을 분석하였다. 분석결과 도시재생사업활성화 계획의 내용 중 주민주도를 이끌어 낼 수 있는 사회적 재생 내용이 회복력에 긍정적인 영향을 미치는 것으로 나타났다. 계획의 단계에서부터 주민들의 자생력을 제고할 수 있는 내용을 포함한다면 도시의 회복력을 높일 수 있을 것으로 기대된다.

• 한국지하수토양환경학회지:지하수토양환경
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• 제27권5호
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• pp.10-17
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• 2022

Inorganic acids such as HCl, HNO₃, and H₂SO₄ have been commonly applied to soil washing of heavy metals-contaminated soil due to their cost-effectiveness. However, implementing the 'Chemical Substance Control Act' requires off-site risk assessment of the chemicals used in the soil washing. Therefore, in this study, organic acids or Fe(III)-based washing agents were evaluated to replace commonly used inorganic acids. Ferric removed heavy metals via H⁺ generated by hydrolysis, which is similar to the HCl used in the control group. Oxalic acid and citric acid were effective to remove Cu, Zn, and Cd from soil. Organic acids could not remove Pb because they could form Pb-organic acid complexes with low solubility. Furthermore, Pb could be adsorbed onto the iron-organic acid complex on the soil surface. Ferric could remove exchangeable-carbonate, Fe-Mn hydroxide, and organic matter and sulfides bound heavy metals (F1, F2, and F3). Organic acids could remove the exchangeable-carbonate and Fe-Mn hydroxide bound metals (F1&F2). Therefore, this research shows that the fractionation of heavy metals in the soil and the properties of washing agents should be considered in the selection of agents in the process design.