The dihydrofolate reductase (dhfr) promoter contains cis-acting element for the transcription factors Spl and E2F. Transcription of dhfr gene shows maximal activity during the Gl/S phase of cell cycle. The member of the Spl transcriptional factor family can act as both negative and positive regulators of gene expression. There was a report that Spl-Rb and E2F4-pl30 complexes cooperate to establish stable repression of dhfr gene expression in CHOC400 cells. Here, we examined the role of HDAC in dhfr, cyclin E, and cyclin A gene regulation using the histone deacetylation inhibitor, trichostatin A (TSA) in U2OS and C33A cells, a Rb-positive human osteosarcoma cell line, and a Rb-negative cervical carcinoma cell line, respectively. When the dhfr promoter constructs were applied in U2OS cells, TSA markedly stimulated over 14-fold of dhfr promoter activity through dhfr-Spl sites by the deletion of an E2F element. In contrast, the deletion of dhfr-Spl binding sites completely abolished promoter stimulation by TSA. The dhfr promoter activity including dhfr-Spl sites increased only 2-fold in C33A cells. Promoter activity containing only dhfr-E2F site did not have much effect by the treatment of TSA in both U2OS and C33A cells. On the other hand, treatment with TSA induced significantly mRNA expression of dhfr and cyclin E, whereas levels of cyclin A decreased in U2OS cells, but had no effect in C33A cells. These results indicate that TSA have contradictory effect, activation of dhfr and cyclin E genes on Gl phase, and down-regulation of cyclin A on G2 phase through transcriptional regulation in U2OS cells.
${\times}$Populus albaglandulosa has been needed optimum stand density according to various site and its wood usage. It is assumed that optimum stand density can be estimated by investigating of response of ${\times}$P. albaglandulosa to the light factor of stand. For that reason, the photosynthesis of ${\times}$Populus albaglandulosa grown under the controlled planting density was studied in relation to its leaf age by the aid of the Infrared gas analyzer. Rate of net photosynthesis was smaller in matured leaves than young leaves below $8^{\circ}C$, while, it was larger than young leaves above $8^{\circ}C$. Temperature for the maximum net photosynthesis of young leaves and old leaves was about $30^{\circ}C$ and $25^{\circ}C$ respectively. Saturated light intensity varied slightly as leaf age from 28 Klux to 35 Klux, but net photosynthesis rate in the range of light intensity showed deep differences. Old leaves marked the lowest rate, $1.6\;CO_2\;mg/dm^2/hr$, young leaves marked the medium rate, 1.7 to $2.2\;CO_2\;mg/dm^2/hr$, and matured leaves marked the most efficient photosynthesis, 2.9 to $3.5\;CO_2\;mg/dm^2/hr$. Young leaves of 5 days old had the highest light compensation point, while matured leaves of 35 days-old had the lowest point. Rates of dark respiration in both young leaves and old leaves were higher than that of matured leaves. Trees which were planted at space $80cm{\times}80cm$ showed productive assimilation function over the one-third of height where relative light intensity is 35%.
In Jeju Island which has peculiarity for its geological features and hydrology system, hydrological factor analysis for the effective water management is necessary. Because in-situ hydro-meteorological data is affected by surrounding environment, the in-situ dataset could not be the spatially representative for the study area. For this reason, remote sensing data may be used to overcome the limit of the in-situ data. In this study, applicability assessment of MOD16 evapotranspiration data, Globas Land Data Assimilation System (GLDAS) based evapotranspiration/soil moisture data, and Advanced SCATterometer (ASCAT) soil moisture product which were evaluated their applicability on other study areas was conducted. In the case of evapotranspiration, comparison with total precipitation and flux-tower based evapotranspiration were conducted. And for soil moisture, 6 in-situ data and ASCAT soil moisture product were compared on each site. As a result, 57% of annual precipitation was calculated as evapotranspiration, and the correlation coefficient between MOD16 evapotranspiration and GLDAS evapotranspiration was 0.759, which was a robust value. The correlation coefficient was 0.434, indicating a relatively low fit. In the case of soil moisture, in the case of the GLDAS data, the RMSE value was less than 0.05 at all sites compared to the in-situ data, and a statistically significant result was obtained as a result of the significance test of the correlation coefficient. However, for satellite data, RMSE over than 0.05 were found at Wolgak and there was no correlation at Sehwa and Handong points. It is judged that the above results are due to insufficient quality control and spatial representation of the evapotranspiration and soil moisture sensors installed in Jeju Island. It is estimated as the error that appears when adjacent to the coast. Through this study, the necessity of improving the existing ground observation data of hydrometeorological factors is emphasized.
Soil compaction is one of the most important activities in the area of civil works, including road construction, airport construction, port construction and backfilling construction of structures. Soil compaction, particularly in road construction, can be categorized into subgrade compaction and roadbed compaction, and is significant work that when done poorly can serve as a factor causing poor construction due to a lack of compaction. Currently, there are many different types of compaction tests, and the plate bearing test and the unit weight of soil test based on the sand cone method are commonly used to measure the degree of compaction, but many other methods are under development as it is difficult to secure economic efficiency. For the purpose of this research, a portable penetration meter called the Free-Fall Penetration Test (FFPT) was developed and manufactured. In this study, a homogeneous sample was obtained from the construction site and soil was classified through a sieve analysis test in order to perform grain size analysis and a specific gravity test for an indoor test. The principle of FFPT is that the penetration needle installed at the tip of an object put into free fall using gravity is used to measure the depth of penetration into the road surface after subgrade or roadbed compaction has been completed; the degree of compaction is obtained through the unit weight of soil test according to the sand cone method and the relationship between the degree of compaction and the depth of the penetration needle is verified. The maximum allowable grain size of soil is 2.36 mm. For $A_1$ compaction, a trend line was developed using the result of the test performed from a drop height of 10 cm, and coefficient of determination of the trend line was $R^2=0.8677$, while for $D_2$ compaction, coefficient of determination of the trend line was $R^2=0.9815$ when testing at a drop height of 20 cm. Free fall test was carried out with the drop height adjusted from 10 cm to 50 cm at increments of 10 cm. This study intends to compare and analyze the correlation between the degree of compaction obtained from the unit weight of soil test based on the sand cone method and the depth of penetration of the penetration needle obtained from the FFPT meter. As such, it is expected that a portable penetration tester will make it easy to test the degree of compaction at many construction sites, and will lead to a reduction in time, equipment, and manpower which are the disadvantages of the current degree of compaction test, ultimately contributing to accurate and simple measurements of the degree of compaction as well as greater economic feasibility.
Inflammation is the most common condition in the human body. Tissue damage triggers inflammation, together with vasodilation and increased blood flow at the inflamed site, resulting in edema. Inflammatory responses are also triggered by lipopolysaccharide (LPS), a Toll-like receptor Enterococcus faecalis, a gram-positive organism, has been reported to possess immunomodulatory and preventive activities; however, its use may present risks of sepsis and other systemic infections. Heat-killed Enterococcus faecalis (EF-2001) has been reported to induce antitumor activity, but its effects on inflammation are not known. In the present study, we investigated the effect of EF-2001 on LPS-induced macrophage inflammatory responses. EF-2001 treatment reduced nitric oxide (NO) production, indicating suppression of inflammatory reactions. EF-2001 showed no cytotoxicity in macrophages. Further investigation of the anti-inflammatory mechanism of EF-2001 indicated that EF-2001 reduced the LPS-induced expression of inducible nitric oxide synthase and cyclooxygenase-2. EF-2001 also reduced f the LPS induction of several inflammatory molecules involved in the nuclear factor-${\kappa}B$ ($NF-{\kappa}B$) and mitogen-activated protein kinase pathways, including ERK, JNK, and p38 phosphorylation, in a concentration-dependent manner. Additionally, EF-2001 inhibited Akt phosphorylation and increased the expression of the inhibitory ${\kappa}B$ ($I{\kappa}B$) protein, an inhibitor of $NF-{\kappa}B$. EF-2001 also inhibited the nuclear translocation of p65. These results suggest that EF-2001 has anti-inflammatory properties and may be useful for treating inflammatory diseases.
The Northern Song Dynasty Period (北宋時代) was a drastic transitional era in all aspect of Chinese society including the politico-economic system, ideology and cultural trait. These changes that began in the late Tang (唐) Dynasty Period accomplished in the Northern Song Dynasty. In this phase, the fundamental change influenced in all institutional area; and among them, the capital city planning and its associating building technology to pile stone walls shows one of the significant change of those time. Based on the geographical factor, confluences of many rivers, the Kaifeing (開封) area where the BianLieng palace had developed as a political and economical centre since the Tang Dynasty when the Grand Canal was constructed. According to archaeological researches, the central city structure of Dongjing Walled Town was begun to plan in the late Tang Dynasty and formed in Five Dynasties. The fundamental functional change of city completed in the Midnorthern Song Dynasty. In spite of the relatively late beginning of archaeological investigations to Kaifeng Walled Town and Dongjing Walled Town due to unfavourable natural environment, excavations inaugurated since 1981 have achieved the significant investigations including the actual measurement and excavation to the outer wall, the preliminary excavation to the inner city area, the investigation and excavation to the royal palace of Song and the survey to the royal palace of King Zho in the Ming (明) Dynasty. These surveys have provide important data to reconstruct the 변량 palace, and elucidate the characteristics of city plan in the Dongjing Walled Town and the institutional change of capital city plan of the Northern Song Dynasty. The basic layout of Dongjing Walled Town reflect the realisation of ideality of the late Chinese medieval capital city structure that establish the commercial and economic centre based on the intensification of emperor's power by means of the organisation of ethical institution and the development of commercial economy. Firstly, the central place of the Kaifeng area is encircled with triple walls. This emphasise the authority of emperor located on the summit in the hierarchical ethic system succeeding to the main capital city plan of the late phase of ancient China. Secondly, the location of Dongjing Walled Town was decided by the transport network and the commercial function and defence function. Thirdly, this site shows the change of city structure and landscape of the Northern Song Dynasty. The closed Fengri (坊里: block) system transferred the open Jiexiang (街巷: road) system. Fourthly, the capital city was characterised by the free market trade and the diversification of market place. Fifthly, a convenient transport network in the Bian River, a centre of the Grand Canals, enabled to construct the Kaifeng Walled Town. Therefore, the Northern Song Dynasty continuously accomplished the developed water system as concerning about the utilisation of waterways after the construction of city.
The zooplankton community and environmental factor were investigated on a weekly basis from March to November 2015 in Lake Paldang, Korea. The seasonal succession of zooplankton community structure was influenced by hydraulic and hydrological factors such as inflow, outflow and rainfall. However, the hydraulic retention time in 2015 (16.3 day) was affected by the periods of water shortage that had continued since 2014 and increased substantially compared to 2013 (7.3 day). Therefore, the inflow and outflow discharge were decreased, and the water quality (COD, BOD, TOC, TP, Chl-a) of Lake Paldang (St.1) was the same characteristics as the river type Bukhan river (St.3), compared with the lake type Namhan river (St.2) and Gyeongan stream (St.4). Zooplankton community dominated by rotifers (Keratella cochlearis, Synchaeta oblonga) in spring (March to May). However, Copepod (Nauplius) and Cladoceran (Bosmina longirostris) dominated in St.4. In summer (June to August), there was a few strong rainfall event and the highest number of individuals dominated by Keratella cochlearis (Rotifera) and Difflugia corona (Protozoa) were shown during the study period. In autumn (October to November), the water temperature was decreased with decrease in the total number of individuals showing Nauplius (Copepoda) as the dominant species. As a result of the statistical analysis about zooplankton variation in environmental factors, the continuous periods of water shortage increased the hydraulic retention time and showed different characteristic for each site. St.1, St.3 and St.2, St.4 are shown in the same group (p<0.05), showing the each characteristics of river type and lake type. Therefore, the water quality of catchment area and distribution of zooplankton community would be attributed to hydraulic and hydrological factors.
Journal of the Korean Institute of Traditional Landscape Architecture
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v.29
no.3
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pp.61-77
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2011
This study focused on relevant rock names related to Sinsun(神仙) which had been settled as Taoist traces were combined with places. While interpreting major features of Sundoism relevant rocks, it also discussed types and places of rocks reflected in their names by considering distinct characteristics of landscape characters that ancestors viewed through the rocks or on the rocks. Conclusion of this study is summarized as follows. 1. Among the rock names related to Sinsun, the most frequently discovered one was Sinsunbawi(52) and followed by Sinsunbong(神仙峰: 38), Sinsundae(神仙臺: 31). Other than these, there were Gangsundae(降仙臺: 12), Sunyoodae (仙遊臺: 10) and Sasundae(四仙臺: 5). 2. In the name of Sinsundae, 'Dae(臺)' ascertains that it was located in greatly superb place in the aspects of viewpoint and appreciation where landscape superiority and overlook scenery were fair and outstanding. 3. Sinsunbong was named for a peak of mountain. At the same time, it implied a notion of worship with images of 'merging with sky' or 'looking up.' Most of time, Sinsunbong indicated the tallest rock in the mountain chain. 4. A significant number of Sinsunbong had names where legends of Sinsun's Go game or descent were originated from. It shows that 'Sinsun(仙) and Go game' used to be very important motives for folk etymology of Sinsun related rocks. Along with the Sinsundae, a number of Sinsunbawi were also turned out to exist in land and ocean with excellent marine view. 5. According to analysis of their altitudes and heights of the peaks where the rocks belong to, Sinsunbong, Sinsundae and Sinsunbawi were in order. It might indicate that the rocks were located on top of mountain or that Sinsunbong represented the mountain itself. Compared to this, Sinsundae was located in where distant panoramic views were overlooked. It was not necessarily to be in peak but in where with a great view like Taoist world. On the other hand, Sinsunbawi was located in where has fine scenery and great valley not so far from villages, which proved its name had been influenced by place feature not altitude. 6. Feature of rock with Sinsun related name is to comprise visual stability of worship object with close linkage to attitude of worshiper. Considering its deep connection with communicative method of worship object and worshiper, seemingly it was main factor to lead folk etymology of rocks with Sinsun related names. 7. Rock is an object with the greatest implication of Sinsun imagination and Sinsun rocks show most clearly the fact that Taoism, which used to be considered as inaccessible, had been actualized in a visual and realistic manner with the change of time.
Journal of the Korean Institute of Traditional Landscape Architecture
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v.29
no.4
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pp.27-39
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2011
The study on Musan twelve peaks of Yongho garden in Jinju, Gyeongnam was anticipated to provide data and implication for reproducing similar spaces and modern changes in terms of design factor since it is the prototype of traditional mount for overcoming monotonous geographical features and intriguing changes and interests. The study analyzed and interpreted the symbolism of twelve peaks, principles of space composition and function and effect of visual construction that were pursued by the builder in terms of landscape view, which results are as following. The center of Yongho garden, Yonghoji(龍虎池) is a typical man-made pond for a supportive feng shui feature. It is a supporting equipment to complete the state of feng shui, and the result of strengthening the completion through the connection with the dragon-related name of the place. The shape of Musan twelve peaks looks like an oval form of Geumseongsan(金星山), 2~3.5m in height and 6~12m in diameter. Peaks are estimated as 1.5~3.7m(2.4m in average) in height, $35{\sim}138m^2$($73.4m^2$ in average) in area, and $30.7{\sim}115.0m^3$($62.5m^3$ in average) in volume. Given that Yonghojeong(龍虎亭), Soseon(小船), the site of main building and Yongsanjae(龍山齋) stand in line, Yonghoji was presumed as the state of enlightenment through ascribing the meaning to virtue and secularity. For the intention of realizing Musan twelve peaks, the builder probably had mounted twelve peaks forming the body of dragons with crossing the point corresponding to a head of tiger, and located Musan twelve peaks and Yonghojeong with a representation of dragons holding Cintamani rising into the sky in the center. The middle area near Musan twelve peaks surrounded by peaks like Geumseongsan running north and south shows a multi-structure of multilayer, maintaining the similarity centering on Yonghoji. It is considered the intention of mount planned at the time of Musan twelve peaks construction, caused by similar form harmony. Internalization of progressive realization through concealment and exposure, enframement effect and spatial order like prospect-refuge theory in the mount of Musan twelve peaks is considered the reflection of the intention to increase depth of the view and expectancy through the various degree of exposure and surroundings of each peak and the colorful combination of viewing and shutting. The "closed view" by Musan twelve peaks creates interesting, vivid and attractive recognition of the view, which is more effective in bringing depth of the view and interests in terms of the geographical design, particularly the area around Yonghoji. Moreover, it was identified that the combination of peaks can be formed resulted from the view configuration concerning the location through multilayer effect reveals an island through the other one when viewed from Yonghojeong.
The purpose of this study was to provide baseline ecological data for the conservation of the Manchurian trout habitat through the investigation of the growth status of Brachymystax lenok tsinlingensis, and Pearson's correlation analysis (PCA) between the B. lenok tsinlingensis population and the use of the land around Gyebangcheon stream. Sampling was conducted twice in July, September, and October 2018. During the July and September surveys, 882 individuals belonging to 13 species from six families were collected. The dominant species was Rhynchocypris kumgangensis and the subdominant species was Zacco koreanus. The total number of B. lenok tsinlingensis collected was 99. The results of the length-weight relationship in the B. lenok tsinlingensis population were analyzed with a regression coefficient b value of 3.1272 and a condition factor (k) value of 0.0006. Therefore, the growth condition of B. lenok tsinlingensis was regarded as fairly good. The QHEI(Qualitative habitat evaluation index) value in the B. lenok tsinlingensis habitat was 119.5(±0.5)-153.5(±0.5), indicating optimal-suboptimal conditions. As a result of the HIS (Habitat suitability index) analysis, it was confirmed that the optimal habitat for B. lenok tsinlingensis was 0.45-0.55m and >1 m in water depth, 0.55-0.65 m s-1 in water velocity, and boulder in the substrate. The ratio of the land use in this study site was analyzed as 66.26-96.31% for forest and grassland areas, 0.00-23.79% for agricultural areas, 0.00-4.19% for urbanized areas, and 3.69-8.87% for others. Correlation analysis of the number of B. lenok tsinlingensis and various factors revealed statistically significant correlations between QHEI and forest and grassland areas, agricultural areas, and urbanized areas.
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