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Regulatory Mechanism of Cytochrome P450IIE in the Rat with Hepatic Injury and Ketosis (간장장해와 Ketosis시에 Cytochrome P450IIE의 Regulatory Mechanism에 관한 연구)

  • 윤여표;강원식;이세창;손동환
    • Journal of Environmental Health Sciences
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    • v.19 no.3
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    • pp.58-63
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    • 1993
  • In order to elucidate the alteration of drug-metabolizing enzymes and mechanism in the animal with hepatic injury and ketosis, the regulation of P450IIE was studied in the rats with heaptic injury caused by CCl$_4$ and with ketosis caused by streptozotocin and high-fat diet. P450IIE expression in liver was examined by the combination of enzyme activities, Western immunoblot, and mRNA Northern blot analyses using specific polyclonal antibody and cDNA probe for P450IIE. Enzyme activity and amounts of immunoreactive P450IIE were rapidly decreased in a time-dependent manner after a single dose of CCl$_4$ . However, the decreases in P450IIE enzyme activity and immunoreactive protein by CCl$_4$ were not accompanied by a decline in its mRNA level. The data thus suggested a post-translational reduction of P450IIE by CCl$_4$. The enzyme activities (aniline hydroxylase) in hepatic microsomes were elevated about 2-3-fold by streptozotocin and feeding with a high fat diet. This increases in enzyme activities were also accompanied by 3-fold increases in immunoreactive P450IIE protein and its mRNA. Our data thus indicated that P450IIE induction during the ketosis appears to be due to pretranslational activation.

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A Broadband Microstrip Array Antenna for PCS/IMT-2000 Base-Station (PCS/IMT-2000 기지국용 광대역 마이크로스트립 배열 안테나)

  • 김태우;최재훈
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.26 no.11B
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    • pp.1620-1627
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    • 2001
  • In this paper, a broadband microstrip antenna for PCS and IMT-2000 service is designed. To obtain the broadband characteristics of an antenna, we utilized the multi-layered structure composed of two foam material layers, parasitic element and aperture coupled feeding network. The broadband characteristic is obtained by changing the size of parasitic element and the height of foam materials. In addition to that, the usage of metal layer at the distance of λ/4 from feed-line, back radiation is reduced. The bandwidth of a single element for VSWR less than 1.3 is about 550MHz. The bandwidth of a designed 1$\times$4 array antenna for VSWR less than 1.3 is about 460MHz. The gain of a designed array antenna is about 11.15∼12.15dBi and the front-to-back ratio is about 30dB.

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Treatment of Textile Wastewater by Membrane-Bioreactor Process (막-생물반응조 공정을 이용한 염색폐수의 처리)

  • 강민수;김성수;황규대;강종림
    • Membrane Journal
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    • v.7 no.4
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    • pp.175-182
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    • 1997
  • Membrane-bioreactor process which combines anaerobic-aerobic bioreactor and membrane process, was used to remove refractory organic susbstances and dye molecule in textile wastewater effectively. Direct feeding of raw feed water to membrane process caused serious fouling on membrane. On the other hand, pretreated feed by bioreactor before the membrane process remarkably reduced the fouling and prolonged the membrane life. Removal efficiency and fouling were more dependent on the material property of the membrane rather than the membrane pore size and structure. Operation mode of hollow fiber membrane module and linear velocity in the hollow fiber influenced the ramoval efficiency and the water flux of the membrane. The combined membrane- bioreactor process was more effective in treating the textile wastewater than each single process.

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Vacuum Plasma Sprayed NiTiZrSiSn Coating (진공 열 플라즈마 용사공정을 통한 NiTiZrSiSn 벌크 비정질 코팅 형성)

  • Yoon, Sang-Hoon;Kim, June-Seob;Kim, Soo-Ki;Lee, Chang-Hee
    • Journal of Welding and Joining
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    • v.25 no.4
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    • pp.42-48
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    • 2007
  • An inert gas atomized NiTiZrSiSn bulk metallic glass feedstock was sprayed onto the copper plate using vacuum plasma spraying process. In order to change the in-flight particle energy, that is, thermal energy, the hydrogen gas flow rate in plasma gas mixture was increased at the constant flow rate of argon gas. Coating and single pass spraying bead were produced with the least feeding rate. Regardless of the plasma gas composition, fully melted through unmelted particle could be observed on the overlay coating. However, the frequency of the unmelted particle number density was increased with the decrease of the hydrogen gas flow rate. The amorphous phase fraction within coating was also affected by the number density of the unmelted particle.

END SN Antibody Titers of Sows and Piglets Vaccinated with Living HC Vaccine During '83 Hog Cholera Outbreaks in Korea ('83 돈(豚)콜레라 유행시(流行時)의 면역모돈(免疫母豚)과 자돈(仔豚)의 END혈청중화항체가(血淸中和抗體價) 조사(調査))

  • Jeon, Yun-seong;Yeh, Jae-gil;Seo, Ik-soo
    • Korean Journal of Veterinary Research
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    • v.25 no.1
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    • pp.69-75
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    • 1985
  • Hog cholera serum neutralizing antibody of piglets and sows were titrated by means of END SN method. The piglets of a variety of ages, precolostrally immunized with LOM living HC vaccine were subjected to the test. The sows were vaccinated with lapinized living HC vaccine after 25 days from the parturition. Throughout the studies the following results were obtained and summarised. 1. Hog cholera antibody titers of inbred sows immunized with lapinized living HC vaccine after 25 days from parturition were high except Hampshire group(Table 2). 2. Sows, different stage of the pregnancy or the day of parturition, and of 3 way crossed, that were immunized with lapinized living HC vaccine have shown no significant difference on HC antibody titer(Table 2, 3). 3. HC antibody titers of piglets, immunized with a single dose of LOM HC vaccine before feeding colostrum, were high in case of the younger group(1 week) compare to the older(7 week) (Table 4). 4. The piglets that were booster immunized with LOM HC vaccine at the age of 7 weeks have shown an inconsistent antibody titers(Table 5).

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Factors affecting nitrite build-up in an intermittently decanted extended aeration process for wastewater treatment (하수처리를 위한 간헐 방류식 장기폭기 공정에서 아질산염의 축적에 영향을 미치는 인자)

  • Ahn, Kyu-Hong;Park, Ki-Young;Lee, Hyung-Jib
    • Journal of Korean Society of Water and Wastewater
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    • v.13 no.1
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    • pp.51-60
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    • 1999
  • An intermittently-aerated, intermittently-decanted single-reactor process (KIDEA process : KIST intermittently decanted extended aeration process) was applied for nitrogen removal from wastewater. Synthetic wastewater with chemical oxygen demand (COD): nitrogen (N) ratio of approximately 5.25: 1 was used. The average COD removal efficiency reached above 95%, and under optimal conditions nitrogen removal efficiency also reached above 90%. This process consisted of 72 minute aeration, 48 minute settling and 24 minute effluent decanting with continuous feeding of influent wastewater from the bottom of the reactor, and did not require a separate anoxic mixing-phase. In this process, nitritation ($1^{st}$ step of nitrification) was induced but nitratation($2^{nd}$ step of nitrification) was suppressed. Main factors responsible for the accumulation of nitrite ion in the experimental condition were free ammonium and dissolved oxygen. This condition of nitrite build-up accelerated by continuous feed flow in the bottom of the KIDEA reactor because of high concentration of ammonia nitrogen in the influent. This research provides one of answers to control nitrate build-up.

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Effects of Methly Group Deficiency on Hepatic Lipid Peroxidation in Diethylnitrosamine and 2-Acetylaminofluorene Treated Rats (메틸기 결핍이 Diethylnitrosamine과 2-Acetylaminofluorene을 투여한 쥐 간의 지질과산화도에 미치는 영향)

  • 김현아
    • Journal of Nutrition and Health
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    • v.25 no.2
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    • pp.116-122
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    • 1992
  • This study determined hepatic microsomal lipid peroxide values glucose 6-phosphatase NA-DPH-cytochrome P450 reductase and cytosolic glutathione S-transferase activites to examine the effects of methyl group deficiency on hepatic lipid peroxidation in rats treated with diethylni-trosamine(DEN) and 2-acetylamionfluorene(AAF) Weanling sprague Dawley male rats were fed the diet with methyl group supplemented or deficient. Two weeks after feeding rate were injected with a single of 200mg/kg body weight DEN intraperitoneally and after four weeks 0.02% AAF containing diets were fed for two weeks. Animals were sacrificed at 6th week. Microsomal lipid peroxide values were tended to increase in methyl group deficiency(MD). Especially in case of carcinogen tratments lipid peroxide values were increased significantly in MD. Microsomal glucose 6-phophatase activities were decreased by MD and carcinogens and in MD with carcinogen group (MD+C) the enzyme activites were the lowest Glucose 6-phosphatase activities were negatively correlated with lipid peroxidation. Microsomal NADPH-cytochrome P450 reductase activities were the highest in MD+C and correlated positively with lipid peroxidation. Cytosolic glutathione S-transferase activities were the highest in MD+C Methyl group deficiency induces lipid peroxidation especially in case of being exposed to carcinogens. Therefore the results suggest that lipid peroxidation may be one of the meachanisms of carcinogensis by methyl group deficiency.

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The Effect of Various Levels of Pectin on the Absorption of Vitamin B$_{12}$ in Rats (흰 쥐에 있어서 식이내 상이한 수준의 팩틴이 비타민 B$_{12}$의 흡수에 미치는 영향)

  • Kim, Jung-In
    • Journal of Nutrition and Health
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    • v.23 no.6
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    • pp.427-433
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    • 1990
  • The effect of short term feeding of various levels of pectin on vitamin B$_{12}$ absorption was studied. Rats fed fiber-free(FF) diet were divided into FF, 2% pectin, 5% pectin or 10% pectin diet group prior to the vitamin B$_{12}$ absorption test. On the day of the absorption test, absorption of a single oral dose of 57-Co-vitamin B12 was measured while rats were consuming their assigned diet. 5 and 10% pectin diet significantly inhibited vitamin B$_{12}$ absorption when compared with FF diet. Pectin intake was inversely correlated with the absorption of vitamin B$_{12}$.

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A cadaveric study on variations in branching pattern of external carotid artery

  • Devadas, Deepa;Pillay, Minnie;Sukumaran, Tintu Thottiyil
    • Anatomy and Cell Biology
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    • v.51 no.4
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    • pp.225-231
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    • 2018
  • Variations in the vascular anatomy of the carotid triangle have been reported in current scientific literature. The carotid arteries, being the major feeding arteries of the head and neck deserve special importance and protection from iatrogenic injury during radiological evaluations and surgical interventions. The present study was carried out over a period of 4 years from 2012-2016 to assess the variant anatomy of external carotid artery. The external carotid artery and its branches were dissected bilaterally in 40 formalin embalmed cadavers. The external carotid artery was traced from its origin to termination and variations in the branching pattern as well as the level of the carotid bifurcation were observed and analysed. A higher carotid bifurcation was observed in 25% cases. The linguofacial trunk was the commonest variation noted in the branching pattern seen in 20% cases. A single case of unilateral thyrolinguofacial trunk was also observed. The external carotid artery gave rise to accessory branches in 7.5% cases namely the superior laryngeal, accessory ascending pharyngeal and masseteric branches. A slender branch to the internal jugular vein was also observed in one case. These findings may provide further insight into the understanding of the vascular anatomy of the carotid triangle to the curious student, the discerning radiologist and the vigilant surgeon to avert complications and help improve overall treatment outcome.

Composition and Abundance of Wood-Boring Beetles Inhabited by Pine Trees

  • Park, Yonghwan;Jang, Taewoong;Won, Daesung;Kim, Jongkuk
    • Journal of Forest and Environmental Science
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    • v.35 no.3
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    • pp.189-196
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    • 2019
  • Plants are consumed by a myriad of organisms that compete for resources. Direct interactions among multiple plant-feeding organisms in a single host can range for each species from positive to negative. Wood-boring beetle faces a number of biotic and abiotic constraints that interfere with the good prospects from the tree. Biotic factors, including arthropod pests and diseases, and abiotic factors, such as drought and water-logging, are the major constraints affecting the species. The present study aimed to provide basic data for analyzing forest health, identify the kinds of wood-boring beetles in the central part of Korea. Our second goal was to analyze the species composition and diversity of regional communities and to examine. A total of 10,461 individual wood-boring beetles belonging to 8 families and 50 species attracted to trap trees in the pine forests were recorded during the study period on study sites. The results of the analysis of collected species showed that the community structure on all study sites was similar. Seasonal occurrences of dominant wood-boring beetles (5 species) from each study site showed the highest number of all species, except for Siphalinus gigas in May, followed by a gradual decline, and the largest number of Siphalinus gigas appeared in June. The similarity index of species composition was relatively high, ranging from 0.75 to 0.90 for each study site.