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Effect of in vitro testicular spermatozoa culture on pregnancy outcomes: an experience at a single university hospital

  • Lee, Jisun;Yoo, Jung Hyeon;Lee, Jae Hun;Ahn, Hyun Soo;Hwang, Kyung Joo;Kim, Miran
    • Journal of Yeungnam Medical Science
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    • v.38 no.1
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    • pp.53-59
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    • 2021
  • Background: There are no guidelines for the optimal incubation time or temperature to improve pregnancy outcomes in testicular sperm extraction-intracytoplasmic sperm injection (TESE-ICSI) cycles. We aimed to evaluate whether a 24-hour in vitro culture of testicular spermatozoa affects pregnancy outcomes in TESE-ICSI cycles. Methods: This was a retrospective study of 83 TESE-ICSI cycles using testicular spermatozoa in 46 couples with male partners suffering from nonobstructive or obstructive azoospermia. Sperm retrieval was performed either on the oocyte retrieval (OR) day (65 cycles in 33 couples; group A) or on the day before OR (18 cycles in 13 couples; group B) followed by in vitro culture for 24 hours. The clinical characteristics and pregnancy outcomes, including the number of retrieved oocytes, fertilization rates, embryo transfer rates, implantation and clinical pregnancy rates, were compared between the two groups. Results: There were no differences in terms of clinical characteristics except for the levels of luteinizing hormone (LH) in males. Group B had higher LH levels than group A (4.56±1.24 IU/L vs. 3.67±1.07 IU/L, p= 0.017). Group B showed higher fertilization rate (72.4%±32.1% vs. 59.2%±21.7%, p=0.045), implantation rate (35.0%±34.1% vs. 14.0%±21.5%, p=0.010), pregnancy rate per cycle (80% vs. 39%, p=0.033), and clinical pregnancy rate per cycle (80% vs. 37.5%, p=0.024) than those of group A. Conclusion: Testicular sperm retrieval performed on the day before OR followed by in vitro culture can potentially improve pregnancy outcomes.

A Study on the Seamline Estimation for Mosaicking of KOMPSAT-3 Images (KOMPSAT-3 영상 모자이킹을 위한 경계선 추정 방법에 대한 연구)

  • Kim, Hyun-ho;Jung, Jaehun;Lee, Donghan;Seo, Doochun
    • Korean Journal of Remote Sensing
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    • v.36 no.6_2
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    • pp.1537-1549
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    • 2020
  • The ground sample distance of KOMPSAT-3 is 0.7 m for panchromatic band, 2.8 m for multi-spectral band, and the swath width of KOMPSAT-3 is 16 km. Therefore, an image of an area wider than the swath width (16 km) cannot be acquired with a single scanning. Thus, after scanning multiple areas in units of swath width, the acquired images should be made into one image. At this time, the necessary algorithm is called image mosaicking or image stitching, and is used for cartography. Mosaic algorithm generally consists of the following 4 steps: (1) Feature extraction and matching, (2) Radiometric balancing, (3) Seamline estimation, and (4) Image blending. In this paper, we have studied an effective seamline estimation method for satellite images. As a result, we can estimate the seamline more accurately than the existing method, and the heterogeneity of the mosaiced images was minimized.

Which anchorage device is the best during retraction of anterior teeth? An overview of systematic reviews

  • Yassir, Yassir A.;Nabbat, Sarah A.;McIntyre, Grant T.;Bearn, David R.
    • The korean journal of orthodontics
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    • v.52 no.3
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    • pp.220-235
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    • 2022
  • Objective: To evaluate the available evidence regarding the clinical effectiveness of different types of anchorage devices. Methods: A comprehensive literature search of different electronic databases was conducted for systematic reviews investigating different anchorage methods published up to April 15, 2021. Any ongoing systematic reviews were searched using PROSPERO, and a grey literature search was undertaken using Google Scholar and OpenGrey. No language restriction was applied. Screening, quality assessment, and data extraction were performed independently by two authors. Information was categorized and narratively synthesized for the key findings from moderate- and high-quality reviews. Results: Fourteen systematic reviews were included (11 were of moderate/high quality). Skeletal anchorage with miniscrews was associated with less anchorage loss (and sometimes with anchorage gain). Similarly, skeletal anchorage was more effective in retracting anterior teeth and intruding incisors and molars, resulting in minor vertical skeletal changes and improvements in the soft tissue profile. However, insufficient evidence was obtained for the preference of any anchorage method in terms of the duration of treatment, number of appointments, quality of treatment, patient perception, or adverse effects. The effectiveness of skeletal anchorage can be enhanced when: directly loaded, used in the mandible rather than the maxilla, used buccally rather than palatally, using dual rather than single miniscrews, used for en-masse retraction, and in adults. Conclusions: The level of evidence regarding anchorage effectiveness is moderate. Nevertheless, compared to conventional anchorage, skeletal anchorage can be used with more anchorage preservation. Further high-quality randomized clinical trials are required to confirm these findings.

Long Song Type Classification based on Lyrics

  • Namjil, Bayarsaikhan;Ganbaatar, Nandinbilig;Batsuuri, Suvdaa
    • Journal of Multimedia Information System
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    • v.9 no.2
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    • pp.113-120
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    • 2022
  • Mongolian folk songs are inspired by Mongolian labor songs and are classified into long and short songs. Mongolian long songs have ancient origins, are rich in legends, and are a great source of folklore. So it was inscribed by UNESCO in 2008. Mongolian written literature is formed under the direct influence of oral literature. Mongolian long song has 3 classes: ayzam, suman, and besreg by their lyrics and structure. In ayzam long song, the world perfectly embodies the philosophical nature of world phenomena and the nature of human life. Suman long song has a wide range of topics such as the common way of life, respect for ancestors, respect for fathers, respect for mountains and water, livestock and animal husbandry, as well as the history of Mongolia. Besreg long songs are dominated by commanded and trained characters. In this paper, we proposed a method to classify their 3 types of long songs using machine learning, based on their lyrics structures without semantic information. We collected lyrics of over 80 long songs and extracted 11 features from every single song. The features are the name of a song, number of the verse, number of lines, number of words, general value, double value, elapsed time of verse, elapsed time of 5 words, and the longest elapsed time of 1 word, full text, and type label. In experimental results, our proposed features show on average 78% recognition rates in function type machine learning methods, to classify the ayzam, suman, and besreg classes.

Pharmacokinetic profiles of levofloxacin after intravenous, intramuscular and subcutaneous administration to rabbits (Oryctolagus cuniculus)

  • Sitovs, Andrejs;Voiko, Laura;Kustovs, Dmitrijs;Kovalcuka, Liga;Bandere, Dace;Purvina, Santa;Giorgi, Mario
    • Journal of Veterinary Science
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    • v.21 no.2
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    • pp.32.1-32.13
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    • 2020
  • Levofloxacin pharmacokinetic profiles were evaluated in 6 healthy female rabbits after intravenous (I/V), intramuscular (I/M), or subcutaneous (S/C) administration routes at a single dose of 5 mg/kg in a 3 × 3 cross-over study. Plasma levofloxacin concentrations were detected using a validated Ultra Performance Liquid Chromatography method with a fluorescence detector. Levofloxacin was quantifiable up to 10 h post-drug administration. Mean AUC0-last values of 9.03 ± 2.66, 9.07 ± 1.80, and 9.28 ± 1.56 mg/h*L were obtained via I/V, I/M, and S/C, respectively. Plasma clearance was 0.6 mL/g*h after I/V administration. Peak plasma concentrations using the I/M and S/C routes were 3.33 ± 0.39 and 2.91 ± 0.56 ㎍/mL. Bioavailability values, after extravascular administration were complete, - 105% ± 27% (I/M) and 118% ± 40% (S/C). Average extraction ratio of levofloxacin after I/V administration was 7%. Additionally, levofloxacin administration effects on tear production and osmolarity were evaluated. Tear osmolarity decreased within 48 h post-drug administration. All 3 levofloxacin administration routes produced similar pharmacokinetic profiles. The studied dose is unlikely to be effective in rabbits; however, it was calculated that a daily dose of 29 mg/kg appears effective for I/V administration for pathogens with MIC < 0.5 ㎍/mL.

Effects of Horsetail, Alfalfa, Ortie, Chêne and Aleppo oak as Potential Hemostatic Agents on Laboratory Coagulation Tests

  • Sina Ahmadianfar;Nahid Mehrabi;Saeed Mohammadi;Ali Sobhanizadeh;Alireza Moradabadi;Ali Noroozi-Aghideh
    • Natural Product Sciences
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    • v.29 no.1
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    • pp.42-49
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    • 2023
  • This study investigated the effect of ethanol extracts of horsetail, alfalfa, ortie, chêne and aleppo oak on blood coagulation in vitro. Extraction was performed by the maceration method. Extracts were mixed with platelet and plasma, then prothrombin time (PT), activated partial thromboplastin time (APTT) and platelet aggregation tests were conducted. Alfalfa extract had a dose-dependent effect on the PT. Ortie, and horsetail, reduced the PT significantly compared to control group. Alfalfa, horsetail, and ortie reduced the APTT, but their effect was insignificant compared to the control group. The pooled extract showed the highest effect compared to the single extracts in a dose-dependent manner. Horsetail and alfalfa induced platelet aggregation in response to arachidonic acid but not in response to collagen. In the case of ortie, no aggregation occurred regarding the arachidonic acid, and incomplete was observed in response to collagen. Interestingly, blood clotting occurred immediately after adding the chêne, aleppo oak and the pooled extract, and therefore platelet poor plasma (PPP) and platelet rich plasma (PRP) became jelly. Generally, chêne and aleppo oak, as well as pooled extract, were more effective in inducing both primary and secondary coagulation pathways via shortening the PT and APTT, and induction of platelet aggregation.

Quantitative Analysis and Enantiomeric Separation of Ephedra Alkaloids in Ma Huang Related Products by HPLC-DAD and UPLC-MS/MS

  • Kyoung-Moon Han;Jinwoo Hwang;Sun Hee Lee;Boreum Park;Hyungil Kim;Sun Young Baek
    • Natural Product Sciences
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    • v.28 no.4
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    • pp.168-180
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    • 2022
  • Ephedra is a genus of the Ephedraceae family and is found in temperate regions, such as Central Asia and Europe. Among the various ephedra species, Ma Huang (Ephedra herb) is derived from the aerial parts of Ephedra sinica S tapf, Ephedra equisetina Bunge, and Ephedra intermedia Schrenk & C.A. Mey. Ma Huang contains various ephedra alkaloids, including (-)-ephedrine, (+)-pseudoephedrine, (-)-norephedrine, (+)-norpseudoephedrine, (-)-methylephedrine, and (+)-methylpseudoephedrine, which are found naturally as single enantiomers, although they can be prepared as racemates. Although the use of Ma Huang in foods is prohibited in Korea, products containing Ma Huang can be imported, and so it is necessary to develop a suitable analytical technique for the detection of Ma Huang in foods. Herein, we report the development of analytical methods for the detection of ephedra alkaloids in products containing Ma Huang. Following sample purification by solid phase extraction, quantitative analysis was performed using ultra-performance liquid chromatography-triple quadrupole mass spectrometry (UPLC-MS/MS). Additionally, the enantiomers were successfully separated using HPLC-DAD. We successfully analyzed various food samples, where the ephedra alkaloids were qualitatively and quantitatively determined, and the enantiomers were separated. It is expected that these methods may contribute toward preventing the distribution of illegal products containing Ma Huang.

Communication performance of selective combining frequency diversity with maximum likelihood estimation in underwater multipath frequency selective channels (수중 다중경로 주파수 선택적 채널에서 최대우도추정을 적용한 선택적합성 주파수 다이버시티의 통신 성능)

  • Lee, Chaehui;Park, Kyu-Chil;Park, Jihyun
    • The Journal of the Acoustical Society of Korea
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    • v.41 no.2
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    • pp.143-149
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    • 2022
  • In this paper, we evaluate the underwater frequency diversity communication performance of Selective Combination (SC) using Maximum Likelihood Estimation (MLE). In an underwater multipath frequency selective channel, destructive interference fading due to delay spread of a received signal affects the increase in error and Signal to Noise Ratio (SNR) variability of an underwater acoustic communication. Selective Combination frequency diversity using a single sensor is applied as a transmission performance improvement technique according to the frequency selectivity of a channel. In the sea experiment applying MLE for SC decision value extraction, we evaluate the performance of SC frequency diversity and MLE-SC frequency diversity. In experiment result, we confirm through experiment that the Bit Error Rate (BER) is relatively lower when the decision value extracted through MLE-SC is applied than when the SC decision value is fixed.

Classification of Fall Crops Using Unmanned Aerial Vehicle Based Image and Support Vector Machine Model - Focusing on Idam-ri, Goesan-gun, Chungcheongbuk-do - (무인기 기반 영상과 SVM 모델을 이용한 가을수확 작물 분류 - 충북 괴산군 이담리 지역을 중심으로 -)

  • Jeong, Chan-Hee;Go, Seung-Hwan;Park, Jong-Hwa
    • Journal of Korean Society of Rural Planning
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    • v.28 no.1
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    • pp.57-69
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    • 2022
  • Crop classification is very important for estimating crop yield and figuring out accurate cultivation area. The purpose of this study is to classify crops harvested in fall in Idam-ri, Goesan-gun, Chungcheongbuk-do by using unmanned aerial vehicle (UAV) images and support vector machine (SVM) model. The study proceeded in the order of image acquisition, variable extraction, model building, and evaluation. First, RGB and multispectral image were acquired on September 13, 2021. Independent variables which were applied to Farm-Map, consisted gray level co-occurrence matrix (GLCM)-based texture characteristics by using RGB images, and multispectral reflectance data. The crop classification model was built using texture characteristics and reflectance data, and finally, accuracy evaluation was performed using the error matrix. As a result of the study, the classification model consisted of four types to compare the classification accuracy according to the combination of independent variables. The result of four types of model analysis, recursive feature elimination (RFE) model showed the highest accuracy with an overall accuracy (OA) of 88.64%, Kappa coefficient of 0.84. UAV-based RGB and multispectral images effectively classified cabbage, rice and soybean when the SVM model was applied. The results of this study provided capacity usefully in classifying crops using single-period images. These technologies are expected to improve the accuracy and efficiency of crop cultivation area surveys by supplementing additional data learning, and to provide basic data for estimating crop yields.

Multi-classification Sensitive Image Detection Method Based on Lightweight Convolutional Neural Network

  • Yueheng Mao;Bin Song;Zhiyong Zhang;Wenhou Yang;Yu Lan
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.17 no.5
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    • pp.1433-1449
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    • 2023
  • In recent years, the rapid development of social networks has led to a rapid increase in the amount of information available on the Internet, which contains a large amount of sensitive information related to pornography, politics, and terrorism. In the aspect of sensitive image detection, the existing machine learning algorithms are confronted with problems such as large model size, long training time, and slow detection speed when auditing and supervising. In order to detect sensitive images more accurately and quickly, this paper proposes a multiclassification sensitive image detection method based on lightweight Convolutional Neural Network. On the basis of the EfficientNet model, this method combines the Ghost Module idea of the GhostNet model and adds the SE channel attention mechanism in the Ghost Module for feature extraction training. The experimental results on the sensitive image data set constructed in this paper show that the accuracy of the proposed method in sensitive information detection is 94.46% higher than that of the similar methods. Then, the model is pruned through an ablation experiment, and the activation function is replaced by Hard-Swish, which reduces the parameters of the original model by 54.67%. Under the condition of ensuring accuracy, the detection time of a single image is reduced from 8.88ms to 6.37ms. The results of the experiment demonstrate that the method put forward has successfully enhanced the precision of identifying multi-class sensitive images, significantly decreased the number of parameters in the model, and achieved higher accuracy than comparable algorithms while using a more lightweight model design.