• The Korean Journal of Physiology and Pharmacology
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• v.4 no.1
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• pp.25-31
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• 2000

ATP-sensitive potassium channels ($K_{ATP}$ channels) play an important role in insulin secretion from pancreatic beta cells. We have investigated the effect of propofol on $K_{ATP}$ channels in cultured single pancreatic beta cells of rats. Channel activity was recorded from membrane patches using the patch-clamp technique. In the inside-out configuration bath-applied propofol inhibited the $K_{ATP}$ channel activities in a dose-dependent manner. The half-maximal inhibition dose (ED50) was $48.6{\pm}8.4\;{\mu}M$ and the Hill coefficient was $0.73{\pm}0.11.$ Single channel conductance calculated from the slope of the relationship between single channel current and pipette potential $(+20{\sim}+100\;mV)$ was not significantly altered by propofol $(control:\;60.0{\pm}2.7\;pS,\;0.1\;mM\;propofol:\;58.7{\pm}3.5\;pS).$ However, mean closed time was surely increased. Above results indicate that propofol blocks the $K_{ATP}$ channels in the pancreatic beta cells in the range of its blood concentrations during anesthesia, suggesting a possible effect on insulin secretion and blood glucose level.

• Structural Engineering and Mechanics
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• v.74 no.3
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• pp.445-455
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• 2020

Shear lag phenomenon has long been taken into consideration in various structural codes; however, the AISC provisions have not proposed any specific equation to calculate the shear lag ratio in some cases such as fillet-welded connections of front-to-front double channel sections. Moreover, those equations and formulas proposed by structural codes are based on the studies that were conducted on riveted and bolted connections, and can be applied to single channel sections whilst using them for fillet-welded double channels would be extremely conservative due to the symmetrical shape and the fact that bending moments will not develop in the gusset plate, resulting in less stress concentration. Numerical models are used in the present study to focus on parametric investigation of the shear lag effect on fillet-welded tension connection of double channel section to a gusset plate. The connection length, the eccentricity of axial load, the free length and the thickness of gusset plate are considered as the key factors in this study. The results are then compared to the estimates driven from the AISC-LRFD provisions and alternative equations are proposed.

• The Korean Journal of Physiology and Pharmacology
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• v.7 no.1
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• pp.15-23
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• 2003

Cellular redox state is known to be perturbed during ischemia and that $Ca^{2+}$ and $K^2$ channels have been shown to have functional thiol groups. In this study, the properties of thiol redox modulation of the ATP-sensitive $K^2$ ($K_{ATP}$) channel were examined in rabbit ventricular myocytes. Rabbit ventricular myocytes were isolated using a Langendorff column for coronary perfusion and collagenase. Single-channel currents were measured in excised membrane patch configuration of patch-clamp technique. The thiol oxidizing agent 5,5'-dithio-bis-(2-nitro-benzoic acid) (DTNB) inhibited the channel activity, and the inhibitory effect of DTNB was reversed by dithiothreitol (disulfide reducing agent; DTT). DTT itself did not have any effect on the channel activity. However, in the patches excised from the metabolically compromised cells, DTT increased the channel activity. DTT had no effect on the inhibitory action by ATP, showing that thiol oxidation was not involved in the blocking mechanism of ATP. There were no statistical difference in the single channel conductance for the oxidized and reduced states of the channel. Analysis of the open and closed time distributions showed that DTNB had no effect on open and closed time distributions shorter than 4 ms. On the other hand, DTNB decreased the life time of bursts and increased the interburst interval. N-ethylmaleimide (NEM), a substance that reacts with thiol groups of cystein residues in proteins, induced irreversible closure of the channel. The thiol oxidizing agents (DTNB, NEM) inhibited of the $K_{ATP}$ channel only, when added to the cytoplasmic side. The results suggested that metabolism-induced changes in the thiol redox can also modulate $K_{ATP}$ channel activity and that a modulatory site of thiol redox may be located on the cytoplasmic side of the $K_{ATP}$ channel in rabbit ventricular myocytes.

• Proceedings of the KSME Conference
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• 2001.06d
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• pp.337-342
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• 2001

A numerical study of three-dimensional fluid flow and heat transfer in the metering section of a single screw extruder has been performed. The mathematical model for the screw channel is simplified by unwound channel and fixing the coordinate system to the screw. The pressure boundary and the prescribed mass flow rate conditions are imposed on the inlet and outlet, respectively. The commercial code STAR-CD based on the finite volume method is used to obtain the results of the present work. The computation of the reverse flow, which cannot be computed by the marching-type 3-D model, is performed in the present study.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.29 no.8
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• pp.408-418
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• 2017

Supercritical carbon dioxide ($sCO_2$) power system is emerging technology because of its high cycle efficiency and compactness. Meanwhile, PCHE (Printed Circuit Heat Exchanger) is gaining attention in $sCO_2$ power system technology because PCHE with high pressure-resistance and larger heat transfer surface per unit volume is fundamentally needed. Thermo-fluidic characteristics of $sCO_2$ in the micro channel of PCHE should be investigated. In this study, heat transfer characteristics of $sCO_2$ of various inlet conditions and cross-sectional shapes of single micro channel were investigated experimentally. Experiment was conducted at supercritical state of higher than critical temperature and pressure. Test sections were made of copper and hydraulic diameter was 1 mm. Convective heat transfer coefficients were measured according to each interval of the channel and pressure drop was also measured. Convective heat transfer coefficients from experimental data were compared with existing correlation. In this study, using measured data, a new empirical correlation to predict near critical region heat transfer coefficient is developed and suggested. Test results of single channel will be used for design of PCHE.

• The Korean Journal of Physiology and Pharmacology
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• v.12 no.2
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• pp.73-77
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• 2008

Recent studies have documented that testosterone relaxes several smooth muscles by modulating $K^+$ channel activities. Smooth muscles of seminal vesicles playa fundamental role in ejaculation, which might involve testosterone. This study was aimed to assess the role of testosterone in seminal vesicular motility by studying its effects on contractile agents and on the ion channels of single vesicular myocytes in a rabbit model. The contractile responses of circular smooth muscle strips of rabbit seminal vesicles to norepinephrine ($10{\mu}M$), a high concentration of KCI (70 mM), and testosterone ($10{\mu}M$) were observed. Single vesicular myocytes of rabbit were isolated using proteolytic enzymes including collagenase and papain. Inside-out, attached, and whole-cell configurations were examined using the patch clamp technique. The applications of $10{\mu}M$ norepinephrine or 70 mM KCl induced tonic contractions, and $10{\mu}M$ testosterone (pharmacological concentration) evoked dose-dependent relaxations of these precontracted strips. Various $K^+$ channel blockers, such as tetraethylammonium (TEA; $10{\mu}M$), iberiotoxin ($0.1{\mu}M$), 4-aminopyridine (4-AP, $10{\mu}M$), or glibenclamide ($10{\mu}M$) rarely affected these relaxations. Single channel data (of inside-out and attached configurations) of BK channel activity were also hardly affected by testosterone ($10{\mu}M$). On the other hand, however, testosterone reduced L-type $Ca^{2+}$ currents significantly, and found to induce acute relaxation of seminal vesicular smooth muscle and this was mediated, at least in part, by $Ca^{2+}$ current inhibition in rabbit.

• Journal of the Korean Society for Precision Engineering
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• v.18 no.1
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• pp.117-122
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• 2001

Many kinds of channels are used in industrial equipment and production machinery. Although mechanical saw has been used to cut many sorts of channels, there is cost rise problem because of low productivity. Shearing of channel has a special place because it helps to cut expected shape and size easily. A channel cutting die set which can be mounted and used on a hydraulic press is developed to improve the productivity of channel cutting process. Mode for the channel cutting is divided into single cut and double cut method. This study use double cut method, and the developed channel cutting die set is composed of upper and lower die set. Shearing time can be reduced from 40 minutes to 20 seconds using the developed channel cutting die set. The productivity of channel cutting process can be increased with shearing time reduction as well as cost reduction.

• Nuclear Engineering and Technology
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• v.51 no.5
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• pp.1218-1230
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• 2019

A sub-channel solver, named ${\underline{S}}teady$ and ${\underline{T}}ransient$ ${\underline{A}}nalyzer$ for ${\underline{R}}eactor$ ${\underline{T}}hermal$ hydraulics (START), has been developed using the homogenous model for two-phase conditions of light water reactors. The code is developed as a fast and accurate TH-solver for coupled and multi-physics calculations. START has been validated against the NUPEC PWR Sub-channel and Bundle Test (PSBT) database. Tests like single-channel quality and void-fraction for steady state, outlet fluid temperature for steady state, rod-bundle quality and void-fraction for both steady state and transient conditions have been analyzed and compared with experimental values. Results reveal a good accuracy of solution for both steady state and transient scenarios. Axially different values for turbulent mixing coefficient are used based on different grid-spacer types. This provides better results as compared to using a single value of turbulent mixing coefficient. Code-to-code evaluation of PSBT results by the START code compares well with other industrial codes. The START code has been parallelized with the OpenMP algorithm and its numerical performance is evaluated with a large whole PWR core. Scaling study of START shows a good parallel performance.

• Geophysics and Geophysical Exploration
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• v.27 no.2
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• pp.91-107
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• 2024

Single-channel seismic exploration has proven effective in delineating subsurface geological structures using small-scale survey systems. The seismic data acquired through zero- or near-offset methods directly capture subsurface features along the vertical axis, facilitating the construction of corresponding seismic sections. However, substantial noise in single-channel seismic data hampers precise interpretation because of the low signal-to-noise ratio. This study introduces a novel approach that integrate noise reduction and signal enhancement via matrix rank optimization to address this issue. Unlike conventional rank-reduction methods, which retain selected singular values to mitigate random noise, our method optimizes the entire singular value spectrum, thus effectively tackling both random and erratic noises commonly found in environments with low signal-to-noise ratio. Additionally, to enhance the horizontal continuity of seismic events and mitigate signal loss during noise reduction, we introduced an adaptive weighting factor computed from the eigenimage of the seismic section. To access the robustness of the proposed method, we conducted numerical experiments using single-channel Sparker seismic data from the Chukchi Plateau in the Arctic Ocean. The results demonstrated that the seismic sections had significantly improved signal-to-noise ratios and minimal signal loss. These advancements hold promise for enhancing single-channel and high-resolution seismic surveys and aiding in the identification of marine development and submarine geological hazards in domestic coastal areas.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.18 no.12
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• pp.1007-1016
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• 2006

Although the advance in electronic technology enables a large number of circuity to be packed in a small volume, it is simultaneously required to remove the high heat load produced by them. In this study, the heat transfer and pressure drop characteristics of a mini-channel heat exchanger, which is designed for liquid cooling of electronic components, are investigated by varying operating conditions. Water and FC-72 were used as working fluids. The mini-channel heat exchanger was made with circular shape channels having din-meters of 2, 3, and 4 mm in regular intervals, and the channel length was 100 mm. The header and inlet guide pathway to provide uniform inflow were attached at the inlet of the test section. Copper block including the heaters was attached at the sidewall of the test section as a heat source, which provided the heat flux from 5 to $15W/cm^2$. The entrance effects enhanced the heat transfer coefficient in the mini-channel significantly. In addition, the single-phase pressure drop in the mini-channel was very similar to that predicted by the laminar flow correlation except that the transition Re decreased due to flow instability in the entrance region.